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The map label for this gene is ttcA [H]

Identifier: 227081604

GI number: 227081604

Start: 1487023

End: 1487955

Strand: Reverse

Name: ttcA [H]

Synonym: VCM66_1388

Alternate gene names: 227081604

Gene position: 1487955-1487023 (Counterclockwise)

Preceding gene: 227081605

Following gene: 227081600

Centisome position: 51.44

GC content: 44.37

Gene sequence:

>933_bases
ATGACAGCGCAAACTCAAGAGTTGACCAAAGCTCAACAATACAATTTCAATAAGTTGCAAAAACGTATTCGTCGTAATAC
TGGTCAAGCAATTGCAGACTTTAATATGATCGAAGATGGCGACCGGATTATGGTATGTCTATCTGGCGGTAAAGACAGCT
TTACCATGCTGGATATTTTGATGAGCCTGCAGAAAAGTGCGCCTATCTCTTTTGAATTGGTTGCTGTCAATCTCGATCAA
AAACAACCGGGATTTCCTGAGCATGTCTTGCCTGAATACCTAGAGAGTTTGGGGGTTGAGTACAAGATCGTAGAAGAAGA
CACCTACGCCATCGTGCAAGATAAAGTGCCTGAAGGCAAAACTACCTGCGCACTCTGCTCTCGTCTGCGTCGCGGTATCT
TATACCGTACCGCCAAAGAGTTAGGAGCAACCAAAATTGCTTTGGGTCACCATCGTGATGACATCTTAGAAACTCTGTTC
TTGAATATGTTCTATGGCGGCAAAATGAAAGGCATGCCACCTAAGCTAGTATCAGACAACGGCGAACATGTGGTTATTCG
CCCGTTGGCCTACTGCCGCGAGAAAGACATCATCAAATATTCAGATATGCGCGGCTACCCGATCATTCCATGTAACTTGT
GTGGGTCACAGCCTAACTTACAGCGCCAAGCGGTAAAACAGATGCTTAATGACTGGGATAAGCGCTTCCCTGGCCGTATT
GAAACCATGTTTAGAGCCATGCAAAACGTGGTGCCAAGCCACCTTGCAGACTTCTCTTTGTTCGATTTTAAATCGATTGA
TAAAAACTCTGGCGTCATTAATGGTGGTGATATTGGCTTTGATAAAGAAGAAATTGCACCGCAAGTCGTGGAAGATGAAG
ACTTAGTGATGGAGTTTGACCCATCACTCCAGTTAAATGTCACCAATATCTAG

Upstream 100 bases:

>100_bases
GCAGGGATTTTTTATCTCTTCACACTATAGATCTGGCATCTCTGCTATTTATCCGTATCATACGCACTTCATTTTGTTCT
GAGATTTAAGATAGCAAGCA

Downstream 100 bases:

>100_bases
GTGGCTTCAAAAGCATAAAAACAAACAAAGGCTGTCTACTGACAGCCTTTGTTTTATGTCTTTCAATCGCTAAAGCTTAC
TTTTTTGCAGAAGGTAAGTA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MTAQTQELTKAQQYNFNKLQKRIRRNTGQAIADFNMIEDGDRIMVCLSGGKDSFTMLDILMSLQKSAPISFELVAVNLDQ
KQPGFPEHVLPEYLESLGVEYKIVEEDTYAIVQDKVPEGKTTCALCSRLRRGILYRTAKELGATKIALGHHRDDILETLF
LNMFYGGKMKGMPPKLVSDNGEHVVIRPLAYCREKDIIKYSDMRGYPIIPCNLCGSQPNLQRQAVKQMLNDWDKRFPGRI
ETMFRAMQNVVPSHLADFSLFDFKSIDKNSGVINGGDIGFDKEEIAPQVVEDEDLVMEFDPSLQLNVTNI

Sequences:

>Translated_310_residues
MTAQTQELTKAQQYNFNKLQKRIRRNTGQAIADFNMIEDGDRIMVCLSGGKDSFTMLDILMSLQKSAPISFELVAVNLDQ
KQPGFPEHVLPEYLESLGVEYKIVEEDTYAIVQDKVPEGKTTCALCSRLRRGILYRTAKELGATKIALGHHRDDILETLF
LNMFYGGKMKGMPPKLVSDNGEHVVIRPLAYCREKDIIKYSDMRGYPIIPCNLCGSQPNLQRQAVKQMLNDWDKRFPGRI
ETMFRAMQNVVPSHLADFSLFDFKSIDKNSGVINGGDIGFDKEEIAPQVVEDEDLVMEFDPSLQLNVTNI
>Mature_309_residues
TAQTQELTKAQQYNFNKLQKRIRRNTGQAIADFNMIEDGDRIMVCLSGGKDSFTMLDILMSLQKSAPISFELVAVNLDQK
QPGFPEHVLPEYLESLGVEYKIVEEDTYAIVQDKVPEGKTTCALCSRLRRGILYRTAKELGATKIALGHHRDDILETLFL
NMFYGGKMKGMPPKLVSDNGEHVVIRPLAYCREKDIIKYSDMRGYPIIPCNLCGSQPNLQRQAVKQMLNDWDKRFPGRIE
TMFRAMQNVVPSHLADFSLFDFKSIDKNSGVINGGDIGFDKEEIAPQVVEDEDLVMEFDPSLQLNVTNI

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Escherichia coli, GI1787606, Length=312, Percent_Identity=74.6794871794872, Blast_Score=495, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI193211090, Length=250, Percent_Identity=31.6, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17540090, Length=232, Percent_Identity=26.7241379310345, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24651972, Length=222, Percent_Identity=28.3783783783784, Blast_Score=84, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 35121; Mature: 34990

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQTQELTKAQQYNFNKLQKRIRRNTGQAIADFNMIEDGDRIMVCLSGGKDSFTMLDIL
CCCCHHHHHHHHHCCHHHHHHHHHHCCCCEEECCCEECCCCEEEEEEECCCCHHHHHHHH
MSLQKSAPISFELVAVNLDQKQPGFPEHVLPEYLESLGVEYKIVEEDTYAIVQDKVPEGK
HHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCC
TTCALCSRLRRGILYRTAKELGATKIALGHHRDDILETLFLNMFYGGKMKGMPPKLVSDN
HHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
GEHVVIRPLAYCREKDIIKYSDMRGYPIIPCNLCGSQPNLQRQAVKQMLNDWDKRFPGRI
CCEEEECCHHHHCCCCCEEECCCCCCCEEEHHCCCCCCCHHHHHHHHHHHHHHHHCCHHH
ETMFRAMQNVVPSHLADFSLFDFKSIDKNSGVINGGDIGFDKEEIAPQVVEDEDLVMEFD
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEECCCCCCCCHHHHCCCCCCCCCEEEEEC
PSLQLNVTNI
CCEEEEECCC
>Mature Secondary Structure 
TAQTQELTKAQQYNFNKLQKRIRRNTGQAIADFNMIEDGDRIMVCLSGGKDSFTMLDIL
CCCHHHHHHHHHCCHHHHHHHHHHCCCCEEECCCEECCCCEEEEEEECCCCHHHHHHHH
MSLQKSAPISFELVAVNLDQKQPGFPEHVLPEYLESLGVEYKIVEEDTYAIVQDKVPEGK
HHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCC
TTCALCSRLRRGILYRTAKELGATKIALGHHRDDILETLFLNMFYGGKMKGMPPKLVSDN
HHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
GEHVVIRPLAYCREKDIIKYSDMRGYPIIPCNLCGSQPNLQRQAVKQMLNDWDKRFPGRI
CCEEEECCHHHHCCCCCEEECCCCCCCEEEHHCCCCCCCHHHHHHHHHHHHHHHHCCHHH
ETMFRAMQNVVPSHLADFSLFDFKSIDKNSGVINGGDIGFDKEEIAPQVVEDEDLVMEFD
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEECCCCCCCCHHHHCCCCCCCCCEEEEEC
PSLQLNVTNI
CCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA