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The map label for this gene is dnaK [H]

Identifier: 227081545

GI number: 227081545

Start: 1423806

End: 1425701

Strand: Reverse

Name: dnaK [H]

Synonym: VCM66_1329

Alternate gene names: 227081545

Gene position: 1425701-1423806 (Counterclockwise)

Preceding gene: 227081546

Following gene: 227081544

Centisome position: 49.29

GC content: 52.69

Gene sequence:

>1896_bases
ATGGAGATGCCAATTCATTCCGCCAAATACAGTGTAGGTATCGACCTTGGTACAACCCACTGCGTACTCGCTTATCAAGA
TGTACAGAGTGAAGAGAGCCGTGTTGAAGTGATGTCGATTGCGCAAATGACAGCGCCGGGCACTGTGGAAAACCTCAACC
AGCTCGGTTCTTTCGTTTATCAACCCCATGAACATGAAATGGCTGCCGCTTCACGCCGTTTGCCTTGGAGCAGTGAACCA
ACTGCACTGGTCGGTGCAATTGCGCGTAATTTGGGCTCAAAAACCCCGATTCGCCTAGTCGCAAGCGCCAAATCTTGGTT
ATGCCATGCGGGCGTTAATCGTCGCGAAGCCTTTTTGCCATCGGGCAGCCCTGAAGATGTCAGCAAAATTTCCCCTCTGC
GTGCCACTGAACTTTATTTAGAGCACTTACAAGCCGCGTGGGATCATGCGCATCCTGATGCCCCATTAGCTGAACAAGAT
GTCACCATTACCGTTCCTGCTTCGTTTGATCCTGCCGCCCGTGATTTAACCGCGGAAGCGGCACGTAATATTGGCCTGCA
GCACTTTACTCTGCTCGAAGAACCACAAGCGGCGCTGTACAGCTGGATTGATAACCGCCATGAGCAATGGCGCAGTGAAG
TGAGCGTTGGCGATGTGGTGTTAGTGATCGATATCGGCGGCGGTACCACTGACCTCTCACTGGTCGAAGTGACCGAAGAA
GAAGGCAACCTTGCCCTCAAGCGTATTGCCGTTGGCGAACATATTCTGCTCGGTGGCGATAACATGGATCTCGCCCTCGC
CTATCGTCTGAAAATGAAGTTGGCGCAGGAAGGCAAAGAGCTGCAAATGTGGCAAGTACAAGCCATGACACACGCATGCC
GTGATGCAAAAGAAGTACTCCTCAGCCAACCGGATTTGGCCGCAGTGCCGATCGTGGTACCAAGCCGCGGTTCTAAACTG
CTCGGCGCAACACTGAAAACCGAACTGACTCGTGAAGAAGTTCAGCAAACGCTAGTCGATGGTTTCTTCCCAGCAGTTTC
TATCAACGAACATCCACAACACAAAATGCGCGGCGCACTGACTCAGATGAGTTTGCCTTACGCGCAAGATGCCGGCATCA
CTCGCCACATCGCCGCATTTTTAAGTAAACAAGCCAACGCGCAAGGTCAAACCGAAAGCCTGCCTTTCGCGATGGCCGGC
ATTCCGGGCATGGCCGCTCCACAAGCCGATTTCATTAAGCCAAGTGCCATTTTACTTAACGGCGGTGTGCTTAAATCGAC
TCTGCTTGCCGATCGTTTGCTAGAAACCATCAATGCTTGGTTAACTCAGGCCAATGCGGCTCCTGCCAAACTCCTTGCCG
GAGTGGATTTGGATCTGGCAGTGGCACGTGGCGCGGCTTACTACGGCGTTGTGCGTCAAGGTCAAGGGATACGCATCCGT
GGTGGTATTGCTTCGGCTTACTATGTCGGGATTGAAAGTGCGATGCCAGCCATTCCGGGCATGTTACCGCCGATGGAAGC
ACTCTGCGTTGCTCCATTTGGTATGGAAGAAGGCTCGAGCGCGCAAGTCGCGGATCGCCAGTTTGGCCTAGTGATTGGTG
AGCCAGTGCACTTCCAATTCTATGGCTCCACCGTTCGCCGTGATGATGTTGCGGGGACTCACCTTGTCGATTGGGCCAAT
GATGAACTAGACGATCTGCCAACCATTCAGATCACCTTACCTGTTTCTGAAGGTCGCCGAGCAGGAGAAGTGGTTCCGGT
TACGCTGGCATCACGAGTGACTGAGCTCGGTACGCTCTATCTTGAAGCGATTGCGGCTGATAATGGCCAAAAATGGCATG
TGGAATTTGATGTTCGGGATAACCCACAGAACGAACCTACACCACAGCAACATTAA

Upstream 100 bases:

>100_bases
CTCTGATTCACAAAGGTTGGCGTGCAGATTCCATAACCCTGCCGAAACTGGCGGACAATTACGACACTTCTATCCTCGCG
CCTGCGGAGGTAGAACTGTA

Downstream 100 bases:

>100_bases
CAAGACAGTAGCGGCATCCTTCGGGGTGCCGCTCAACCTTTATGAACACGAGGTATCGCATGTCATCACCACGTTTTCTG
GTCGGCATTGATTTAGGCAC

Product: DnaK-related protein

Products: NA

Alternate protein names: HSP70; Heat shock 70 kDa protein; Heat shock protein 70 [H]

Number of amino acids: Translated: 631; Mature: 631

Protein sequence:

>631_residues
MEMPIHSAKYSVGIDLGTTHCVLAYQDVQSEESRVEVMSIAQMTAPGTVENLNQLGSFVYQPHEHEMAAASRRLPWSSEP
TALVGAIARNLGSKTPIRLVASAKSWLCHAGVNRREAFLPSGSPEDVSKISPLRATELYLEHLQAAWDHAHPDAPLAEQD
VTITVPASFDPAARDLTAEAARNIGLQHFTLLEEPQAALYSWIDNRHEQWRSEVSVGDVVLVIDIGGGTTDLSLVEVTEE
EGNLALKRIAVGEHILLGGDNMDLALAYRLKMKLAQEGKELQMWQVQAMTHACRDAKEVLLSQPDLAAVPIVVPSRGSKL
LGATLKTELTREEVQQTLVDGFFPAVSINEHPQHKMRGALTQMSLPYAQDAGITRHIAAFLSKQANAQGQTESLPFAMAG
IPGMAAPQADFIKPSAILLNGGVLKSTLLADRLLETINAWLTQANAAPAKLLAGVDLDLAVARGAAYYGVVRQGQGIRIR
GGIASAYYVGIESAMPAIPGMLPPMEALCVAPFGMEEGSSAQVADRQFGLVIGEPVHFQFYGSTVRRDDVAGTHLVDWAN
DELDDLPTIQITLPVSEGRRAGEVVPVTLASRVTELGTLYLEAIAADNGQKWHVEFDVRDNPQNEPTPQQH

Sequences:

>Translated_631_residues
MEMPIHSAKYSVGIDLGTTHCVLAYQDVQSEESRVEVMSIAQMTAPGTVENLNQLGSFVYQPHEHEMAAASRRLPWSSEP
TALVGAIARNLGSKTPIRLVASAKSWLCHAGVNRREAFLPSGSPEDVSKISPLRATELYLEHLQAAWDHAHPDAPLAEQD
VTITVPASFDPAARDLTAEAARNIGLQHFTLLEEPQAALYSWIDNRHEQWRSEVSVGDVVLVIDIGGGTTDLSLVEVTEE
EGNLALKRIAVGEHILLGGDNMDLALAYRLKMKLAQEGKELQMWQVQAMTHACRDAKEVLLSQPDLAAVPIVVPSRGSKL
LGATLKTELTREEVQQTLVDGFFPAVSINEHPQHKMRGALTQMSLPYAQDAGITRHIAAFLSKQANAQGQTESLPFAMAG
IPGMAAPQADFIKPSAILLNGGVLKSTLLADRLLETINAWLTQANAAPAKLLAGVDLDLAVARGAAYYGVVRQGQGIRIR
GGIASAYYVGIESAMPAIPGMLPPMEALCVAPFGMEEGSSAQVADRQFGLVIGEPVHFQFYGSTVRRDDVAGTHLVDWAN
DELDDLPTIQITLPVSEGRRAGEVVPVTLASRVTELGTLYLEAIAADNGQKWHVEFDVRDNPQNEPTPQQH
>Mature_631_residues
MEMPIHSAKYSVGIDLGTTHCVLAYQDVQSEESRVEVMSIAQMTAPGTVENLNQLGSFVYQPHEHEMAAASRRLPWSSEP
TALVGAIARNLGSKTPIRLVASAKSWLCHAGVNRREAFLPSGSPEDVSKISPLRATELYLEHLQAAWDHAHPDAPLAEQD
VTITVPASFDPAARDLTAEAARNIGLQHFTLLEEPQAALYSWIDNRHEQWRSEVSVGDVVLVIDIGGGTTDLSLVEVTEE
EGNLALKRIAVGEHILLGGDNMDLALAYRLKMKLAQEGKELQMWQVQAMTHACRDAKEVLLSQPDLAAVPIVVPSRGSKL
LGATLKTELTREEVQQTLVDGFFPAVSINEHPQHKMRGALTQMSLPYAQDAGITRHIAAFLSKQANAQGQTESLPFAMAG
IPGMAAPQADFIKPSAILLNGGVLKSTLLADRLLETINAWLTQANAAPAKLLAGVDLDLAVARGAAYYGVVRQGQGIRIR
GGIASAYYVGIESAMPAIPGMLPPMEALCVAPFGMEEGSSAQVADRQFGLVIGEPVHFQFYGSTVRRDDVAGTHLVDWAN
DELDDLPTIQITLPVSEGRRAGEVVPVTLASRVTELGTLYLEAIAADNGQKWHVEFDVRDNPQNEPTPQQH

Specific function: Acts as a chaperone [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Escherichia coli, GI1786196, Length=285, Percent_Identity=25.9649122807018, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stati

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012725
- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 68216; Mature: 68216

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00297 HSP70_1 ; PS00329 HSP70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMPIHSAKYSVGIDLGTTHCVLAYQDVQSEESRVEVMSIAQMTAPGTVENLNQLGSFVY
CCCCCCCCCEEEEEECCCCEEEEEEHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHC
QPHEHEMAAASRRLPWSSEPTALVGAIARNLGSKTPIRLVASAKSWLCHAGVNRREAFLP
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCCCCCEEECC
SGSPEDVSKISPLRATELYLEHLQAAWDHAHPDAPLAEQDVTITVPASFDPAARDLTAEA
CCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHH
ARNIGLQHFTLLEEPQAALYSWIDNRHEQWRSEVSVGDVVLVIDIGGGTTDLSLVEVTEE
HHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEEECC
EGNLALKRIAVGEHILLGGDNMDLALAYRLKMKLAQEGKELQMWQVQAMTHACRDAKEVL
CCCEEEEEEECCCEEEECCCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LSQPDLAAVPIVVPSRGSKLLGATLKTELTREEVQQTLVDGFFPAVSINEHPQHKMRGAL
HCCCCCEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHH
TQMSLPYAQDAGITRHIAAFLSKQANAQGQTESLPFAMAGIPGMAAPQADFIKPSAILLN
HHHCCCCHHCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEE
GGVLKSTLLADRLLETINAWLTQANAAPAKLLAGVDLDLAVARGAAYYGVVRQGQGIRIR
CCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHCCCCEEHEEECCCCEEEE
GGIASAYYVGIESAMPAIPGMLPPMEALCVAPFGMEEGSSAQVADRQFGLVIGEPVHFQF
CCCCEEEEEEHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCEEEEE
YGSTVRRDDVAGTHLVDWANDELDDLPTIQITLPVSEGRRAGEVVPVTLASRVTELGTLY
ECCEECCCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHH
LEAIAADNGQKWHVEFDVRDNPQNEPTPQQH
HHHEECCCCCEEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MEMPIHSAKYSVGIDLGTTHCVLAYQDVQSEESRVEVMSIAQMTAPGTVENLNQLGSFVY
CCCCCCCCCEEEEEECCCCEEEEEEHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHC
QPHEHEMAAASRRLPWSSEPTALVGAIARNLGSKTPIRLVASAKSWLCHAGVNRREAFLP
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCCCCCEEECC
SGSPEDVSKISPLRATELYLEHLQAAWDHAHPDAPLAEQDVTITVPASFDPAARDLTAEA
CCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHH
ARNIGLQHFTLLEEPQAALYSWIDNRHEQWRSEVSVGDVVLVIDIGGGTTDLSLVEVTEE
HHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEEECC
EGNLALKRIAVGEHILLGGDNMDLALAYRLKMKLAQEGKELQMWQVQAMTHACRDAKEVL
CCCEEEEEEECCCEEEECCCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LSQPDLAAVPIVVPSRGSKLLGATLKTELTREEVQQTLVDGFFPAVSINEHPQHKMRGAL
HCCCCCEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHH
TQMSLPYAQDAGITRHIAAFLSKQANAQGQTESLPFAMAGIPGMAAPQADFIKPSAILLN
HHHCCCCHHCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEE
GGVLKSTLLADRLLETINAWLTQANAAPAKLLAGVDLDLAVARGAAYYGVVRQGQGIRIR
CCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHCCCCEEHEEECCCCEEEE
GGIASAYYVGIESAMPAIPGMLPPMEALCVAPFGMEEGSSAQVADRQFGLVIGEPVHFQF
CCCCEEEEEEHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCEEEEE
YGSTVRRDDVAGTHLVDWANDELDDLPTIQITLPVSEGRRAGEVVPVTLASRVTELGTLY
ECCEECCCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHH
LEAIAADNGQKWHVEFDVRDNPQNEPTPQQH
HHHEECCCCCEEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12835416 [H]