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The map label for this gene is ycdT [H]

Identifier: 227081543

GI number: 227081543

Start: 1419628

End: 1420806

Strand: Direct

Name: ycdT [H]

Synonym: VCM66_1327

Alternate gene names: 227081543

Gene position: 1419628-1420806 (Clockwise)

Preceding gene: 227081541

Following gene: 227081547

Centisome position: 49.08

GC content: 43.77

Gene sequence:

>1179_bases
GTGCATTCAGGACGACCAAGCATGATGGATCTTTCTTTTTTAGAAATTGCCACAGTGATAAATAACATCGCATGCAGTGC
AATAGCCGCGCTCTTTCTTTATATGTTAAGAAGCAAGCTGATTGAACATAAATTGGATACGGCCACTTGGTTTGTGCTGC
TGTTTTTGGCTTACGGCTTGGCTTATACCACCTCTTCGCTGCGTTTTATTTTACCGATGGGATTTGCCATCTTTGTCAAT
AACTTGCTCTATCAACTTGGCGGTTATTGCATGCTATTTGCCGTATTGCGCTGGTATCAAAAGCCGATCAAAAGTGGCTT
ATACCTACTGATGATGGCGCACTCCTTGTTGTTTGCTTTGTTGTTATATCTCTTGTTCCGTTTGAATCCTGAAGACATAG
GACTGCGCATCTTGATTGCTTCGTTAAGCTTGTCTTCGGTGTATCTGATTTCTGCAATCGTTGCGGCAAAAAATGGTCAG
CGTGGGCGGCCAGAGCAAACTCTTTTCGCTGTTGTATTGGCAATGATGATGGTCATGCTCTACGTTCCTTTCGTCGCTTA
TCAAACTTTACCTTTGTTGGCGGTATTTCGCGCCTCGACCATAGTGGTGCAGAACCTGTTCTTTTTTATCATTCTTGGCA
CCATGCTAATGCTGTTTTTGTTCGAGGAAATAGAATGGCATCGCTTACGCTCTATTCAAGATGAACTGACAGGCGCGTTT
AACCGTCGTCATTTTAAAGAGCAAGTACAGCAAAAATTGAAGCAGTCTAAGCAGAAATCTATGGTGGCTTTAGTCGATAT
TGACCATTTCAAGCAGATCAATGATTCCTATGGACATGATATTGGTGATTCAGTGATCACTTATGTGGTTCGCCATTTAG
AGTCACTGGCATTAACGGAATGCTTGGTCGCTCGATATGGCGGTGAAGAGTTCGCGATCTTTGCCCGTGAAAATGATTTT
GAGCGAGTATCGCAAGCTCTTGATTCGGTACGTGATGCGATTTCGGTAGGTTTTCATGTTGAACAGCAACCACTCAAAGT
ATCGGTCAGTATCGGCGCGGTGATTTTCGAAAGTTCACATGAATATGGCGAAGTATTACGCCAAGCGGATAAGGCGTTAT
ACCAAGCGAAGCAACAAGGACGAAACCGAATCATGCTGCAAGACGTGAGCGCGAGTTAA

Upstream 100 bases:

>100_bases
TCAGGTCGTTAATGCGAGCTTAAGTTGTTAGATAATTTATATTTTAGTAGTTATTTGGGGAGAAGAGCGCCAAAATGTTG
TTTAAGTTGATTTTATTTTT

Downstream 100 bases:

>100_bases
CTCAGATTGTTTATAGGATGGGAGCCTAACGGCTCCTAATTTCAATACCTGCAACTCCCAGTAGTTTTGGTATATCTGCG
ACTTCAAGTTGTTTGGGCTT

Product: GGDEF family protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MHSGRPSMMDLSFLEIATVINNIACSAIAALFLYMLRSKLIEHKLDTATWFVLLFLAYGLAYTTSSLRFILPMGFAIFVN
NLLYQLGGYCMLFAVLRWYQKPIKSGLYLLMMAHSLLFALLLYLLFRLNPEDIGLRILIASLSLSSVYLISAIVAAKNGQ
RGRPEQTLFAVVLAMMMVMLYVPFVAYQTLPLLAVFRASTIVVQNLFFFIILGTMLMLFLFEEIEWHRLRSIQDELTGAF
NRRHFKEQVQQKLKQSKQKSMVALVDIDHFKQINDSYGHDIGDSVITYVVRHLESLALTECLVARYGGEEFAIFARENDF
ERVSQALDSVRDAISVGFHVEQQPLKVSVSIGAVIFESSHEYGEVLRQADKALYQAKQQGRNRIMLQDVSAS

Sequences:

>Translated_392_residues
MHSGRPSMMDLSFLEIATVINNIACSAIAALFLYMLRSKLIEHKLDTATWFVLLFLAYGLAYTTSSLRFILPMGFAIFVN
NLLYQLGGYCMLFAVLRWYQKPIKSGLYLLMMAHSLLFALLLYLLFRLNPEDIGLRILIASLSLSSVYLISAIVAAKNGQ
RGRPEQTLFAVVLAMMMVMLYVPFVAYQTLPLLAVFRASTIVVQNLFFFIILGTMLMLFLFEEIEWHRLRSIQDELTGAF
NRRHFKEQVQQKLKQSKQKSMVALVDIDHFKQINDSYGHDIGDSVITYVVRHLESLALTECLVARYGGEEFAIFARENDF
ERVSQALDSVRDAISVGFHVEQQPLKVSVSIGAVIFESSHEYGEVLRQADKALYQAKQQGRNRIMLQDVSAS
>Mature_392_residues
MHSGRPSMMDLSFLEIATVINNIACSAIAALFLYMLRSKLIEHKLDTATWFVLLFLAYGLAYTTSSLRFILPMGFAIFVN
NLLYQLGGYCMLFAVLRWYQKPIKSGLYLLMMAHSLLFALLLYLLFRLNPEDIGLRILIASLSLSSVYLISAIVAAKNGQ
RGRPEQTLFAVVLAMMMVMLYVPFVAYQTLPLLAVFRASTIVVQNLFFFIILGTMLMLFLFEEIEWHRLRSIQDELTGAF
NRRHFKEQVQQKLKQSKQKSMVALVDIDHFKQINDSYGHDIGDSVITYVVRHLESLALTECLVARYGGEEFAIFARENDF
ERVSQALDSVRDAISVGFHVEQQPLKVSVSIGAVIFESSHEYGEVLRQADKALYQAKQQGRNRIMLQDVSAS

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=162, Percent_Identity=39.5061728395062, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1786584, Length=163, Percent_Identity=34.9693251533742, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1787802, Length=162, Percent_Identity=34.5679012345679, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081881, Length=185, Percent_Identity=34.5945945945946, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI145693134, Length=186, Percent_Identity=32.258064516129, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788085, Length=175, Percent_Identity=29.1428571428571, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=34.7560975609756, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788381, Length=160, Percent_Identity=30, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082007, Length=179, Percent_Identity=30.7262569832402, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787056, Length=158, Percent_Identity=32.2784810126582, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1787541, Length=215, Percent_Identity=28.3720930232558, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788956, Length=157, Percent_Identity=31.2101910828025, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI87081977, Length=154, Percent_Identity=29.8701298701299, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI87081974, Length=156, Percent_Identity=28.2051282051282, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 44575; Mature: 44575

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]