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The map label for this gene is fumB [H]

Identifier: 227081475

GI number: 227081475

Start: 1342835

End: 1344352

Strand: Direct

Name: fumB [H]

Synonym: VCM66_1259

Alternate gene names: 227081475

Gene position: 1342835-1344352 (Clockwise)

Preceding gene: 227081470

Following gene: 227081476

Centisome position: 46.42

GC content: 51.12

Gene sequence:

>1518_bases
ATGACGGTAATACGCAAGCAAGATGTGATCAGCAGTGTCGCTGATGCGCTGCAGTACATCTCCTACTACCACCCATTAGA
CTTTGTAAAAGCCCTAGAAAAAGCCTACCAACGTGAAGAAAGCCAAGCGGCGAAAGATTCGATAGCGCAGATTTTGATTA
ACTCGCGCATGTCTGCAGAAGGCCACCGCCCCATTTGTCAGGATACGGGTATTGTCACTTGTTTCGTCAATATTGGTATG
GGCGTGCAATGGGATTCGACCGACATGACGGTGCAGCAGATGGTGGATGAGGGCGTGCGCCAAGCGTACACCAACCCAGA
TAACCCACTGCGTGCCTCAGTATTGATGGATCCTGCGGGCAAGCGCATCAATACCAAAGACAATACCCCAGCTGTGGTGC
ACATCAATATGGTGCCTGGCGATAAAGTGGATATTCAAATTGCGGCCAAAGGCGGCGGCAGTGAAAACAAAACCAAGATG
GTGATGCTCAATCCGTCCGATGATATTGCGGAATGGGTAGAAAAAACCGTACCCTTGATGGGCGCGGGCTGGTGTCCACC
GGGCATGCTCGGCATTGGTATTGGCGGTACGGCGGAAAAAGCGGCCGTCCTCGCCAAAGAAGCTTTGATGGAACACATTG
ATATTCATGAGCTTATCGAGCGTGGTCCGCAATATGCGGAAGAAGAGCTGCGTTTAGACATTTTCAACCGTGTGAATAAG
CTCGGTATTGGCGCGCAAGGCCTTGGTGGTTTGACCACAGTGGTTGATGTGAAAATCAAATCGGCACCGACACATGCCGC
TTCTAAGCCTGTCTGCATGATCCCCAACTGTGCGGCGACACGCCACGTGCATTTCACACTCGATGGCAGCGGCCCTGCGC
AGTTAACACCGCCTAAGTTAGAAGATTGGCCACAAATCACTTGGGAAGCGGGCGCGAATACGCGTCGTGTCAATCTTGAT
AACATCACCCGTGAAGAGATCCAAACGTGGAAAACCGGTGAAACGGTTCTGCTGTCTGGCAAGATACTCACTGGTCGTGA
CGCCGCCCATAAACGCATTCAAACCATGCTGCAAAAAGGCGAAAAATTGCCTGACAGTGTCGATTTGAAAGACAAGTTCA
TTTACTACGTAGGTCCTGTGGACGCAGTAGGCGATGAAGTGGTTGGCCCTGCAGGCCCTACAACTTCAACTCGTATGGAT
AAGTTTACCGACATGATGCTGGAAGAGGTCGGCGTCATGGGCATGATTGGTAAAGCTGAGCGCGGCCCAGCTACCGTTGA
ATCGATCAAAAAACACAAAGCTGTTTATTTGATGGCAGTCGGTGGTGCGGCTTACTTAGTGGCGAAAGCGATCAAGAAAG
CTCGCGTAGTCGCGTTTGAAGACTTAGGCATGGAGGCAATCTACGAATTTGAAGTCGAAGATATGCCAGTCACTGTTGCT
GTGGATTCAACGGGCGCGAATGCGCACCAAATCGGTCCAGATACTTGGCGGGTCAAAATCGCTGAAGCGGCCAAGTAA

Upstream 100 bases:

>100_bases
ACAAAGCTGCTACTTAAAGAGTGAGCGTCTCCTTCGGTTTTGGAAGCAGTAATTCACCCAAGTCGCAGTACACGGAAGCA
TAACAATAACGAGGCGAGCA

Downstream 100 bases:

>100_bases
AGGCTTGCCTCTTCTCAGCGAGGAACTTGGCAAAAAATGACAAAGGTGCACGTAAGTTGCACCTTTTTAATAGGATACCT
TTCCTACTCGAAGCTGTAGT

Product: putative fumarate hydratase, class I

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MTVIRKQDVISSVADALQYISYYHPLDFVKALEKAYQREESQAAKDSIAQILINSRMSAEGHRPICQDTGIVTCFVNIGM
GVQWDSTDMTVQQMVDEGVRQAYTNPDNPLRASVLMDPAGKRINTKDNTPAVVHINMVPGDKVDIQIAAKGGGSENKTKM
VMLNPSDDIAEWVEKTVPLMGAGWCPPGMLGIGIGGTAEKAAVLAKEALMEHIDIHELIERGPQYAEEELRLDIFNRVNK
LGIGAQGLGGLTTVVDVKIKSAPTHAASKPVCMIPNCAATRHVHFTLDGSGPAQLTPPKLEDWPQITWEAGANTRRVNLD
NITREEIQTWKTGETVLLSGKILTGRDAAHKRIQTMLQKGEKLPDSVDLKDKFIYYVGPVDAVGDEVVGPAGPTTSTRMD
KFTDMMLEEVGVMGMIGKAERGPATVESIKKHKAVYLMAVGGAAYLVAKAIKKARVVAFEDLGMEAIYEFEVEDMPVTVA
VDSTGANAHQIGPDTWRVKIAEAAK

Sequences:

>Translated_505_residues
MTVIRKQDVISSVADALQYISYYHPLDFVKALEKAYQREESQAAKDSIAQILINSRMSAEGHRPICQDTGIVTCFVNIGM
GVQWDSTDMTVQQMVDEGVRQAYTNPDNPLRASVLMDPAGKRINTKDNTPAVVHINMVPGDKVDIQIAAKGGGSENKTKM
VMLNPSDDIAEWVEKTVPLMGAGWCPPGMLGIGIGGTAEKAAVLAKEALMEHIDIHELIERGPQYAEEELRLDIFNRVNK
LGIGAQGLGGLTTVVDVKIKSAPTHAASKPVCMIPNCAATRHVHFTLDGSGPAQLTPPKLEDWPQITWEAGANTRRVNLD
NITREEIQTWKTGETVLLSGKILTGRDAAHKRIQTMLQKGEKLPDSVDLKDKFIYYVGPVDAVGDEVVGPAGPTTSTRMD
KFTDMMLEEVGVMGMIGKAERGPATVESIKKHKAVYLMAVGGAAYLVAKAIKKARVVAFEDLGMEAIYEFEVEDMPVTVA
VDSTGANAHQIGPDTWRVKIAEAAK
>Mature_504_residues
TVIRKQDVISSVADALQYISYYHPLDFVKALEKAYQREESQAAKDSIAQILINSRMSAEGHRPICQDTGIVTCFVNIGMG
VQWDSTDMTVQQMVDEGVRQAYTNPDNPLRASVLMDPAGKRINTKDNTPAVVHINMVPGDKVDIQIAAKGGGSENKTKMV
MLNPSDDIAEWVEKTVPLMGAGWCPPGMLGIGIGGTAEKAAVLAKEALMEHIDIHELIERGPQYAEEELRLDIFNRVNKL
GIGAQGLGGLTTVVDVKIKSAPTHAASKPVCMIPNCAATRHVHFTLDGSGPAQLTPPKLEDWPQITWEAGANTRRVNLDN
ITREEIQTWKTGETVLLSGKILTGRDAAHKRIQTMLQKGEKLPDSVDLKDKFIYYVGPVDAVGDEVVGPAGPTTSTRMDK
FTDMMLEEVGVMGMIGKAERGPATVESIKKHKAVYLMAVGGAAYLVAKAIKKARVVAFEDLGMEAIYEFEVEDMPVTVAV
DSTGANAHQIGPDTWRVKIAEAAK

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=474, Percent_Identity=32.0675105485232, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1787897, Length=517, Percent_Identity=30.9477756286267, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1789442, Length=275, Percent_Identity=29.4545454545455, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1789443, Length=171, Percent_Identity=34.5029239766082, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54828; Mature: 54696

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIRKQDVISSVADALQYISYYHPLDFVKALEKAYQREESQAAKDSIAQILINSRMSAE
CCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GHRPICQDTGIVTCFVNIGMGVQWDSTDMTVQQMVDEGVRQAYTNPDNPLRASVLMDPAG
CCCCCCCCCCEEEEEEECCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCC
KRINTKDNTPAVVHINMVPGDKVDIQIAAKGGGSENKTKMVMLNPSDDIAEWVEKTVPLM
CCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHCCCC
GAGWCPPGMLGIGIGGTAEKAAVLAKEALMEHIDIHELIERGPQYAEEELRLDIFNRVNK
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHH
LGIGAQGLGGLTTVVDVKIKSAPTHAASKPVCMIPNCAATRHVHFTLDGSGPAQLTPPKL
HCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCEEEEEEEECCCCCCCCCCCCC
EDWPQITWEAGANTRRVNLDNITREEIQTWKTGETVLLSGKILTGRDAAHKRIQTMLQKG
CCCCCEEEECCCCEEEEECCCCCHHHHHHCCCCCEEEEECEEEECCHHHHHHHHHHHHCC
EKLPDSVDLKDKFIYYVGPVDAVGDEVVGPAGPTTSTRMDKFTDMMLEEVGVMGMIGKAE
CCCCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHCCCCCCC
RGPATVESIKKHKAVYLMAVGGAAYLVAKAIKKARVVAFEDLGMEAIYEFEVEDMPVTVA
CCCHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHEEEEHHCCCEEEEEEEECCCEEEEE
VDSTGANAHQIGPDTWRVKIAEAAK
EECCCCCCCCCCCCCEEEEEECCCC
>Mature Secondary Structure 
TVIRKQDVISSVADALQYISYYHPLDFVKALEKAYQREESQAAKDSIAQILINSRMSAE
CCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GHRPICQDTGIVTCFVNIGMGVQWDSTDMTVQQMVDEGVRQAYTNPDNPLRASVLMDPAG
CCCCCCCCCCEEEEEEECCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCC
KRINTKDNTPAVVHINMVPGDKVDIQIAAKGGGSENKTKMVMLNPSDDIAEWVEKTVPLM
CCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHCCCC
GAGWCPPGMLGIGIGGTAEKAAVLAKEALMEHIDIHELIERGPQYAEEELRLDIFNRVNK
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHH
LGIGAQGLGGLTTVVDVKIKSAPTHAASKPVCMIPNCAATRHVHFTLDGSGPAQLTPPKL
HCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCEEEEEEEECCCCCCCCCCCCC
EDWPQITWEAGANTRRVNLDNITREEIQTWKTGETVLLSGKILTGRDAAHKRIQTMLQKG
CCCCCEEEECCCCEEEEECCCCCHHHHHHCCCCCEEEEECEEEECCHHHHHHHHHHHHCC
EKLPDSVDLKDKFIYYVGPVDAVGDEVVGPAGPTTSTRMDKFTDMMLEEVGVMGMIGKAE
CCCCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHCCCCCCC
RGPATVESIKKHKAVYLMAVGGAAYLVAKAIKKARVVAFEDLGMEAIYEFEVEDMPVTVA
CCCHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHEEEEHHCCCEEEEEEEECCCEEEEE
VDSTGANAHQIGPDTWRVKIAEAAK
EECCCCCCCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]