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The map label for this gene is setA [H]

Identifier: 227081433

GI number: 227081433

Start: 1292718

End: 1293932

Strand: Direct

Name: setA [H]

Synonym: VCM66_1216

Alternate gene names: 227081433

Gene position: 1292718-1293932 (Clockwise)

Preceding gene: 227081431

Following gene: 227081434

Centisome position: 44.69

GC content: 48.64

Gene sequence:

>1215_bases
ATGTATCGAGATAAAACCGCCTTACTGTTTATTATGTCGACACTGGTTACGGGTCTATGCGGAGCGTTTTTCTATCCACT
CTCCAGTCTGTTTATTGTTGAGGCGCTCGATGCTTCACCGATGCAACTGAGCCTGTATATGGTGTTGGCAGTCGTCAGCT
CAGTTATCGTGTCGCAGTGGCTTGCACGCCAATCAGATCGTCATTGGCAGCGTAAAACCATCTTATTGGTTTCGTTAATC
TGTTATCTCATCACTGTGGTCAGCTTTATGTTTATTCGCAGCTACAGTCTCGCCATCATGGTGGTGGTACTGTTTGCTAG
CGTGAGTGGCGCGTCATTTGGTCAGCTTTTTGCGCTTGGCCGCGAATACGCCGATCAGCATTTAACTGATAAAACGACCT
TCCTCTCTACCATGCGTGCCGGAATTGCGATTGCTTGGGTATTTGGCCCTCCGGCGGCGTTTATGCTTAAAGCCAGTTTC
GGCTTTAATGCCGCCTTTGCGGTGTCTGCTCTGATAGTATTTTTAGGCATAGTGCTGATTGCTCGCTTCTTGCCAGCAGG
GGTGAAAACCGCCTCAGCCGTTGGAACCACGCCAGTCGAACTTGGACAATATTCACCCCGTAAGCGTGCTTTGATCGTAC
TGTACTGCGTGACCTTGGTATGTACTTTTGGCTCAAACAATCTTTATATCACCAGTATGCCGCTCTATTTATCACGTGAG
TTGATGGTGCCTGAACATTGGCTAGGGTTGCTGTTTGGCACGGCCGCATTGTGTGAAATTCCGGTCATGTTAATGGCGGG
AAAACTTGCCGAGCGATGGGGAACAAATAAATTGCTGATGTTTGGGATCACCAGCGGCATCTTGTTTTACGGTGTGATGC
TGACCCACACTGGGTTTGTTGCCATGATGGCCGCGCAAGTGCTCAATGGCTTTTTTATCGGCGTATGCGCGACGCTAGGC
ATGGTGGTGCTGCAGGACATGATGCAAGACCGATTGGGCACGGCTTCCACCTTATTTTCGAGCATGCTCAGTATCAGTAC
TTTGGTGGCGAGCTTATCGGTTGGCGTAGTCGGTGAGTTTTATAACTATTTCAGTACCTTGTATGTTTCTTTAACTGGTG
CTTTCAGTGCTTTACTGCTGTTGGCGTTGTTTAGTCAACTGCAGCGCAAAGCAATACCAACCGAAGCGGTGCAAAGTGGA
GTCTCGGTTCATTAA

Upstream 100 bases:

>100_bases
AGCCGCCATAATCCCCCCTGATGCTCTAAGTCGTTTAGGGATAAAGCGACGCTGCCAAGTAGGAAGGGGATTCCATCGAA
TATTGCTATGAGGTTACTGC

Downstream 100 bases:

>100_bases
GGGCAAAGAAAAATGCGTTGCGTTGGGATCGCTTGAGGTATTCGCCAAGGTACTCGCTGCGTAATTATTCTCTAAAGTGT
AAAAATTATGTGACCATACT

Product: Sugar efflux transporter A

Products: lactose [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MYRDKTALLFIMSTLVTGLCGAFFYPLSSLFIVEALDASPMQLSLYMVLAVVSSVIVSQWLARQSDRHWQRKTILLVSLI
CYLITVVSFMFIRSYSLAIMVVVLFASVSGASFGQLFALGREYADQHLTDKTTFLSTMRAGIAIAWVFGPPAAFMLKASF
GFNAAFAVSALIVFLGIVLIARFLPAGVKTASAVGTTPVELGQYSPRKRALIVLYCVTLVCTFGSNNLYITSMPLYLSRE
LMVPEHWLGLLFGTAALCEIPVMLMAGKLAERWGTNKLLMFGITSGILFYGVMLTHTGFVAMMAAQVLNGFFIGVCATLG
MVVLQDMMQDRLGTASTLFSSMLSISTLVASLSVGVVGEFYNYFSTLYVSLTGAFSALLLLALFSQLQRKAIPTEAVQSG
VSVH

Sequences:

>Translated_404_residues
MYRDKTALLFIMSTLVTGLCGAFFYPLSSLFIVEALDASPMQLSLYMVLAVVSSVIVSQWLARQSDRHWQRKTILLVSLI
CYLITVVSFMFIRSYSLAIMVVVLFASVSGASFGQLFALGREYADQHLTDKTTFLSTMRAGIAIAWVFGPPAAFMLKASF
GFNAAFAVSALIVFLGIVLIARFLPAGVKTASAVGTTPVELGQYSPRKRALIVLYCVTLVCTFGSNNLYITSMPLYLSRE
LMVPEHWLGLLFGTAALCEIPVMLMAGKLAERWGTNKLLMFGITSGILFYGVMLTHTGFVAMMAAQVLNGFFIGVCATLG
MVVLQDMMQDRLGTASTLFSSMLSISTLVASLSVGVVGEFYNYFSTLYVSLTGAFSALLLLALFSQLQRKAIPTEAVQSG
VSVH
>Mature_404_residues
MYRDKTALLFIMSTLVTGLCGAFFYPLSSLFIVEALDASPMQLSLYMVLAVVSSVIVSQWLARQSDRHWQRKTILLVSLI
CYLITVVSFMFIRSYSLAIMVVVLFASVSGASFGQLFALGREYADQHLTDKTTFLSTMRAGIAIAWVFGPPAAFMLKASF
GFNAAFAVSALIVFLGIVLIARFLPAGVKTASAVGTTPVELGQYSPRKRALIVLYCVTLVCTFGSNNLYITSMPLYLSRE
LMVPEHWLGLLFGTAALCEIPVMLMAGKLAERWGTNKLLMFGITSGILFYGVMLTHTGFVAMMAAQVLNGFFIGVCATLG
MVVLQDMMQDRLGTASTLFSSMLSISTLVASLSVGVVGEFYNYFSTLYVSLTGAFSALLLLALFSQLQRKAIPTEAVQSG
VSVH

Specific function: Involved in the efflux of sugars. The physiological role may be the detoxification of non-metabolizable sugar analogs. Can transport IPTG, lactose and glucose. Has broad substrate specificity, with preferences for glucosides or galactosides with alkyl or

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Set transporter family [H]

Homologues:

Organism=Escherichia coli, GI48994875, Length=361, Percent_Identity=33.7950138504155, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1790091, Length=377, Percent_Identity=32.3607427055703, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1788495, Length=389, Percent_Identity=30.8483290488432, Blast_Score=190, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004750 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43896; Mature: 43896

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRDKTALLFIMSTLVTGLCGAFFYPLSSLFIVEALDASPMQLSLYMVLAVVSSVIVSQW
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LARQSDRHWQRKTILLVSLICYLITVVSFMFIRSYSLAIMVVVLFASVSGASFGQLFALG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
REYADQHLTDKTTFLSTMRAGIAIAWVFGPPAAFMLKASFGFNAAFAVSALIVFLGIVLI
HHHHHHHCCHHHHHHHHHHHCCEEEEEECCCHHHHEEECCCCCHHHHHHHHHHHHHHHHH
ARFLPAGVKTASAVGTTPVELGQYSPRKRALIVLYCVTLVCTFGSNNLYITSMPLYLSRE
HHHHCCCCCHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHH
LMVPEHWLGLLFGTAALCEIPVMLMAGKLAERWGTNKLLMFGITSGILFYGVMLTHTGFV
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHH
AMMAAQVLNGFFIGVCATLGMVVLQDMMQDRLGTASTLFSSMLSISTLVASLSVGVVGEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YNYFSTLYVSLTGAFSALLLLALFSQLQRKAIPTEAVQSGVSVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCC
>Mature Secondary Structure
MYRDKTALLFIMSTLVTGLCGAFFYPLSSLFIVEALDASPMQLSLYMVLAVVSSVIVSQW
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LARQSDRHWQRKTILLVSLICYLITVVSFMFIRSYSLAIMVVVLFASVSGASFGQLFALG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
REYADQHLTDKTTFLSTMRAGIAIAWVFGPPAAFMLKASFGFNAAFAVSALIVFLGIVLI
HHHHHHHCCHHHHHHHHHHHCCEEEEEECCCHHHHEEECCCCCHHHHHHHHHHHHHHHHH
ARFLPAGVKTASAVGTTPVELGQYSPRKRALIVLYCVTLVCTFGSNNLYITSMPLYLSRE
HHHHCCCCCHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHH
LMVPEHWLGLLFGTAALCEIPVMLMAGKLAERWGTNKLLMFGITSGILFYGVMLTHTGFV
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHH
AMMAAQVLNGFFIGVCATLGMVVLQDMMQDRLGTASTLFSSMLSISTLVASLSVGVVGEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YNYFSTLYVSLTGAFSALLLLALFSQLQRKAIPTEAVQSGVSVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactose [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: lactose [Cytoplasm] + Proton [Periplasm] = lactose [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 10209755; 10438463 [H]