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The map label for this gene is sbcB [H]

Identifier: 227081406

GI number: 227081406

Start: 1264628

End: 1266052

Strand: Reverse

Name: sbcB [H]

Synonym: VCM66_1189

Alternate gene names: 227081406

Gene position: 1266052-1264628 (Counterclockwise)

Preceding gene: 227081407

Following gene: 227081405

Centisome position: 43.77

GC content: 50.81

Gene sequence:

>1425_bases
ATGCAGCAAGAACACCAGCCCACATTCTTCTTTTTCGACTACGAGACTTGGGGAGTCAATCCCGCAAAAGATCGTCCTAG
TCAGTTTGCGGGTGTGCGTACCGATATGGACTTCAACATCATCGGCGAGCCTTTGGTAATTTACTGTCAGCTGCCCAACG
ACTATTTGCCCGCCCCTGAGGCCGCATTGATCACAGGGATCACCCCTCAAAAAGCCAATAGCCAAGGTCTCGCGGAGCCC
GAATTTATCGCGCGTATTCATGCTGAGCTCTCCACACCCAATACCATCAACCTAGGTTATAACAGCATACGTTTTGATGA
TGAAGTGACGCGCTACACCTGCTATCGCAACTTTATCGACCCCTATGCTTGGAGCTGGCAACACGGCAATTCCCGTTGGG
ATCTGCTGGATGTGCTTCGCGCGTGTCATGCTTTGCGTCCTGAAGGTATCGAGTGGCCGGAAAATGATGACGGCTTCACC
AGCTTTAAACTGGAACATCTTTCTGTTGCCAACGGCATTGAGCACAGCAACGCGCACGATGCGATGGCCGATGTGATTGC
CACCATTGAACTGGCGAAAAAAGTCAAAGCCGCGCAGCCGAAATTGTTCGACTACTTCTTCACTATGCGCCACAAACGAA
AGCTGACAGAACTGATTGATATTGTGAACCAAACTCCGCTGATGCATGTTTCTGGCATGCTTGGGCGTGAGCGTCACTAC
ACTAGCTGGATTGTGCCTATTGCTTGGCATCCAACCAATCAAAATGCGGTGATTGTGGTGGATTTGGGTAAAGACCCTCA
GCCGCTGCTGGAGCTCGATGCCGAAACGCTGCAAGCGCGTCTCTATACGCGGTATGAAGACCTAGCGCCGGATGAGCTAC
CAGTACCCGTGAAATTGGTGCATCTCAATAAATGCCCTATTCTCGCTCCGGCCAAAACCTTGACCGCAGAAAATGCACAA
AAGATTGGTATTGACCGCGAACAGTGTCTCGCGCACTTGAATGTGATTCGTCAACACCCCGAGATTCGTGAAAAGCTGGT
GACGGTATTTAGCCAAGAGCGCGAATTTAACCAAGAGAGTGATGTGGATAGCCAACTCTATGCCGGCTTTTTCTCCCCAG
CCGATCGCGCGGCGATGGAGATCATCCGCACCACTGCACCGGAGAATCTGGGCAGCTTAGCGATCCGGGTGGCCGATAAG
CGCATTGAACCGCTGCTGTTTCGTTATCGTGCGCGCCATTATCCCCACACCTTAACCGATACCGAGCAACGCCGCTGGGC
AGCGCATTGTCGTGACTATTTCGAGCGCAATCTAGAAGGTTATATGTTGAATTTGGAAAATCTGGTGCATGAACATCAAG
CTGATCCAAAGAAAATGGCGATTTTAAAATCCGTCTACCATTACGTGGAAAAACTCGCTTGCTAA

Upstream 100 bases:

>100_bases
GAAAGGCTGAACAAATAATGGGATAGCACAAGGGCAGCAATTGCTATGTCAGGGTCAGGATGGTCTAATAACGGCAATTT
TTAGCGATTTGGTAAGCCAC

Downstream 100 bases:

>100_bases
CATCGTTTATTTTTACTTGCTGATTTTATTGATGATAAAAAAAATAGCTCAATACTGTGTCTCCATGGGACTGATTTTTC
TCTGTCTGTTGGCGGGCATC

Product: exonuclease I

Products: NA

Alternate protein names: Exonuclease I; DNA deoxyribophosphodiesterase; dRPase [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MQQEHQPTFFFFDYETWGVNPAKDRPSQFAGVRTDMDFNIIGEPLVIYCQLPNDYLPAPEAALITGITPQKANSQGLAEP
EFIARIHAELSTPNTINLGYNSIRFDDEVTRYTCYRNFIDPYAWSWQHGNSRWDLLDVLRACHALRPEGIEWPENDDGFT
SFKLEHLSVANGIEHSNAHDAMADVIATIELAKKVKAAQPKLFDYFFTMRHKRKLTELIDIVNQTPLMHVSGMLGRERHY
TSWIVPIAWHPTNQNAVIVVDLGKDPQPLLELDAETLQARLYTRYEDLAPDELPVPVKLVHLNKCPILAPAKTLTAENAQ
KIGIDREQCLAHLNVIRQHPEIREKLVTVFSQEREFNQESDVDSQLYAGFFSPADRAAMEIIRTTAPENLGSLAIRVADK
RIEPLLFRYRARHYPHTLTDTEQRRWAAHCRDYFERNLEGYMLNLENLVHEHQADPKKMAILKSVYHYVEKLAC

Sequences:

>Translated_474_residues
MQQEHQPTFFFFDYETWGVNPAKDRPSQFAGVRTDMDFNIIGEPLVIYCQLPNDYLPAPEAALITGITPQKANSQGLAEP
EFIARIHAELSTPNTINLGYNSIRFDDEVTRYTCYRNFIDPYAWSWQHGNSRWDLLDVLRACHALRPEGIEWPENDDGFT
SFKLEHLSVANGIEHSNAHDAMADVIATIELAKKVKAAQPKLFDYFFTMRHKRKLTELIDIVNQTPLMHVSGMLGRERHY
TSWIVPIAWHPTNQNAVIVVDLGKDPQPLLELDAETLQARLYTRYEDLAPDELPVPVKLVHLNKCPILAPAKTLTAENAQ
KIGIDREQCLAHLNVIRQHPEIREKLVTVFSQEREFNQESDVDSQLYAGFFSPADRAAMEIIRTTAPENLGSLAIRVADK
RIEPLLFRYRARHYPHTLTDTEQRRWAAHCRDYFERNLEGYMLNLENLVHEHQADPKKMAILKSVYHYVEKLAC
>Mature_474_residues
MQQEHQPTFFFFDYETWGVNPAKDRPSQFAGVRTDMDFNIIGEPLVIYCQLPNDYLPAPEAALITGITPQKANSQGLAEP
EFIARIHAELSTPNTINLGYNSIRFDDEVTRYTCYRNFIDPYAWSWQHGNSRWDLLDVLRACHALRPEGIEWPENDDGFT
SFKLEHLSVANGIEHSNAHDAMADVIATIELAKKVKAAQPKLFDYFFTMRHKRKLTELIDIVNQTPLMHVSGMLGRERHY
TSWIVPIAWHPTNQNAVIVVDLGKDPQPLLELDAETLQARLYTRYEDLAPDELPVPVKLVHLNKCPILAPAKTLTAENAQ
KIGIDREQCLAHLNVIRQHPEIREKLVTVFSQEREFNQESDVDSQLYAGFFSPADRAAMEIIRTTAPENLGSLAIRVADK
RIEPLLFRYRARHYPHTLTDTEQRRWAAHCRDYFERNLEGYMLNLENLVHEHQADPKKMAILKSVYHYVEKLAC

Specific function: Also functions as a DNA deoxyribophosphodiesterase that releases deoxyribose-phosphate moieties following the cleavage DNA at an apurinic/apyrimidinic (AP) site by either an AP endonuclease AP lyase [H]

COG id: COG2925

COG function: function code L; Exonuclease I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788321, Length=470, Percent_Identity=59.1489361702128, Blast_Score=580, Evalue=1e-166,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013620
- InterPro:   IPR013520
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T; PF08411 Exonuc_X-T_C [H]

EC number: =3.1.11.1 [H]

Molecular weight: Translated: 54599; Mature: 54599

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQEHQPTFFFFDYETWGVNPAKDRPSQFAGVRTDMDFNIIGEPLVIYCQLPNDYLPAPE
CCCCCCCEEEEEEEEECCCCCCCCCCHHHCCCCCCCCCEECCCCEEEEEECCCCCCCCCC
AALITGITPQKANSQGLAEPEFIARIHAELSTPNTINLGYNSIRFDDEVTRYTCYRNFID
CCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCEECCCHHHHHHHHHHHCC
PYAWSWQHGNSRWDLLDVLRACHALRPEGIEWPENDDGFTSFKLEHLSVANGIEHSNAHD
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHCCCCCCCHHH
AMADVIATIELAKKVKAAQPKLFDYFFTMRHKRKLTELIDIVNQTPLMHVSGMLGRERHY
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
TSWIVPIAWHPTNQNAVIVVDLGKDPQPLLELDAETLQARLYTRYEDLAPDELPVPVKLV
CEEEEEEEEECCCCCEEEEEECCCCCCHHHHHCHHHHHHHHHHHHHHCCCCCCCCCEEEE
HLNKCPILAPAKTLTAENAQKIGIDREQCLAHLNVIRQHPEIREKLVTVFSQEREFNQES
EECCCCCCCCHHHHHHCCHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
DVDSQLYAGFFSPADRAAMEIIRTTAPENLGSLAIRVADKRIEPLLFRYRARHYPHTLTD
CCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCC
TEQRRWAAHCRDYFERNLEGYMLNLENLVHEHQADPKKMAILKSVYHYVEKLAC
CHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQQEHQPTFFFFDYETWGVNPAKDRPSQFAGVRTDMDFNIIGEPLVIYCQLPNDYLPAPE
CCCCCCCEEEEEEEEECCCCCCCCCCHHHCCCCCCCCCEECCCCEEEEEECCCCCCCCCC
AALITGITPQKANSQGLAEPEFIARIHAELSTPNTINLGYNSIRFDDEVTRYTCYRNFID
CCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCEECCCHHHHHHHHHHHCC
PYAWSWQHGNSRWDLLDVLRACHALRPEGIEWPENDDGFTSFKLEHLSVANGIEHSNAHD
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHCCCCCCCHHH
AMADVIATIELAKKVKAAQPKLFDYFFTMRHKRKLTELIDIVNQTPLMHVSGMLGRERHY
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
TSWIVPIAWHPTNQNAVIVVDLGKDPQPLLELDAETLQARLYTRYEDLAPDELPVPVKLV
CEEEEEEEEECCCCCEEEEEECCCCCCHHHHHCHHHHHHHHHHHHHHCCCCCCCCCEEEE
HLNKCPILAPAKTLTAENAQKIGIDREQCLAHLNVIRQHPEIREKLVTVFSQEREFNQES
EECCCCCCCCHHHHHHCCHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
DVDSQLYAGFFSPADRAAMEIIRTTAPENLGSLAIRVADKRIEPLLFRYRARHYPHTLTD
CCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCC
TEQRRWAAHCRDYFERNLEGYMLNLENLVHEHQADPKKMAILKSVYHYVEKLAC
CHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3539937; 9097040; 9278503; 1329027 [H]