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The map label for this gene is purT

Identifier: 227081400

GI number: 227081400

Start: 1260137

End: 1261318

Strand: Reverse

Name: purT

Synonym: VCM66_1183

Alternate gene names: 227081400

Gene position: 1261318-1260137 (Counterclockwise)

Preceding gene: 227081401

Following gene: 227081399

Centisome position: 43.61

GC content: 51.69

Gene sequence:

>1182_bases
TTGTCTATGTTTGGGACCGCGACTCGGGATAACGCAACGCGAGTACTTTTGCTTGGCTCTGGTGAACTTGGTAAGGAAGT
CGCGATTGAGTGTCAGCGCCTTGGCCTCGAAGTCATCGCCTGTGATCGTTACGCCGATGCGCCTGCCATGCAGGTCGCCC
ATCGCAGCCACGTGTTTGATATGCTCGATGCCGATGCACTACAACAAGTGATTGATTTAGAAAAGCCACATTTCGTGGTT
CCAGAAATTGAAGCGATTGCTACCAGCAAGTTAGTGGAGTTAGAAGCGCAAGGCTTAAACGTTGTGCCTACCGCCAATGC
AACTCGCCTCACCATGAACCGTGAAGGCATTCGCCGTTTAGCCGCTGAAGAGTTAGGGTTGCCCACTTCCGCGTATCAAT
TCGCAGACAGCTATGAGAGCTTTGCCGCGGCAGTAGAAACCATCGGTTTACCTTGTGTCTGTAAGCCTGTCATGAGTTCA
TCGGGCAAAGGCCAAAGTGTTATTCGCACACCAGAGCAGATCGAAGCGGCTTGGCAATACGCACAACAAGGTGGACGCAG
TGGCGCTGGCCGAGTGATTGTGGAAGGCTTCGTGGATTTTGATTACGAAATCACACTGCTGACCGTACGCGCTGTGGATG
GCGTCCATTTCTGCGCTCCAATTGGCCATCGCCAAGAAGATGGCGATTACCGCGAGTCATGGCAACCACAAGTGATGTCA
GAAAATGCCATTAAAGCGGCTGAATATGTGGCAGAACAGGTGGTTAACGCGCTTGGTGGTTATGGCTTGTTTGGAGTTGA
ACTGTTTGTGAAAGGTGACAAGGTCATCTTCAACGAAGTTTCCCCTCGCCCACACGATACCGGAATGGTGACGCTCATTT
CTCAAGAGCTCTCTGAATTCGCTTTGCATGTGCGCGCGTTTACTGGTCTACCGATTGGTCAAATCGTGCAATATGGCCCA
TCAGCCTCTGCGGCGATCCTCGGCCAAGGCCAGTCACAGAACATCCAGTTCAATGGACTTGATGACGCCTTAAGCATTCC
TCACACCCAAGTACGTTTATTTGGCAAGCCAGACATCGCGGGTCGTCGTCGCTTAGGTGTTGCCCTGAGCCGAGGAAAAA
CGACGCAAGAAGCCACTGATCGCGCGATTGAGTGTGCGAAAGCTGTGAAGATTCATTACTAA

Upstream 100 bases:

>100_bases
ATCTGACTGGCTACAGCGGGAAGCAAACGTTTTCTTTTGTGCTTTGTTCCAGTATCATGGCCGCTGTTTTTTGCTAATGT
TGACTGAATGACTATAAGGA

Downstream 100 bases:

>100_bases
TTCAGCTCTTTATCTTCATCAGCACTATCAGAAAGCGCGCTCTTTCAGCGCGCTTTTTTCATTTCTACTTAGTGGCGTTC
AAAGAGTCAGAGTCACTCGC

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MSMFGTATRDNATRVLLLGSGELGKEVAIECQRLGLEVIACDRYADAPAMQVAHRSHVFDMLDADALQQVIDLEKPHFVV
PEIEAIATSKLVELEAQGLNVVPTANATRLTMNREGIRRLAAEELGLPTSAYQFADSYESFAAAVETIGLPCVCKPVMSS
SGKGQSVIRTPEQIEAAWQYAQQGGRSGAGRVIVEGFVDFDYEITLLTVRAVDGVHFCAPIGHRQEDGDYRESWQPQVMS
ENAIKAAEYVAEQVVNALGGYGLFGVELFVKGDKVIFNEVSPRPHDTGMVTLISQELSEFALHVRAFTGLPIGQIVQYGP
SASAAILGQGQSQNIQFNGLDDALSIPHTQVRLFGKPDIAGRRRLGVALSRGKTTQEATDRAIECAKAVKIHY

Sequences:

>Translated_393_residues
MSMFGTATRDNATRVLLLGSGELGKEVAIECQRLGLEVIACDRYADAPAMQVAHRSHVFDMLDADALQQVIDLEKPHFVV
PEIEAIATSKLVELEAQGLNVVPTANATRLTMNREGIRRLAAEELGLPTSAYQFADSYESFAAAVETIGLPCVCKPVMSS
SGKGQSVIRTPEQIEAAWQYAQQGGRSGAGRVIVEGFVDFDYEITLLTVRAVDGVHFCAPIGHRQEDGDYRESWQPQVMS
ENAIKAAEYVAEQVVNALGGYGLFGVELFVKGDKVIFNEVSPRPHDTGMVTLISQELSEFALHVRAFTGLPIGQIVQYGP
SASAAILGQGQSQNIQFNGLDDALSIPHTQVRLFGKPDIAGRRRLGVALSRGKTTQEATDRAIECAKAVKIHY
>Mature_392_residues
SMFGTATRDNATRVLLLGSGELGKEVAIECQRLGLEVIACDRYADAPAMQVAHRSHVFDMLDADALQQVIDLEKPHFVVP
EIEAIATSKLVELEAQGLNVVPTANATRLTMNREGIRRLAAEELGLPTSAYQFADSYESFAAAVETIGLPCVCKPVMSSS
GKGQSVIRTPEQIEAAWQYAQQGGRSGAGRVIVEGFVDFDYEITLLTVRAVDGVHFCAPIGHRQEDGDYRESWQPQVMSE
NAIKAAEYVAEQVVNALGGYGLFGVELFVKGDKVIFNEVSPRPHDTGMVTLISQELSEFALHVRAFTGLPIGQIVQYGPS
ASAAILGQGQSQNIQFNGLDDALSIPHTQVRLFGKPDIAGRRRLGVALSRGKTTQEATDRAIECAKAVKIHY

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=392, Percent_Identity=70.9183673469388, Blast_Score=569, Evalue=1e-164,
Organism=Escherichia coli, GI1786733, Length=256, Percent_Identity=26.5625, Blast_Score=87, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324702, Length=364, Percent_Identity=24.4505494505494, Blast_Score=103, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_VIBCM (C3LLS4)

Other databases:

- EMBL:   CP001233
- RefSeq:   YP_002809951.1
- ProteinModelPortal:   C3LLS4
- SMR:   C3LLS4
- GeneID:   7772461
- GenomeReviews:   CP001233_GR
- KEGG:   vcm:VCM66_1183
- OMA:   PDYIVPE
- ProtClustDB:   PRK09288
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42576; Mature: 42445

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 82-82 BINDING 114-114 BINDING 155-155 BINDING 203-203 BINDING 267-267 BINDING 279-279 BINDING 286-286 BINDING 356-356

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMFGTATRDNATRVLLLGSGELGKEVAIECQRLGLEVIACDRYADAPAMQVAHRSHVFD
CCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHH
MLDADALQQVIDLEKPHFVVPEIEAIATSKLVELEAQGLNVVPTANATRLTMNREGIRRL
HHCHHHHHHHHCCCCCCEECCCHHHHHHHHHHEEECCCCEEEECCCCEEEEECHHHHHHH
AAEELGLPTSAYQFADSYESFAAAVETIGLPCVCKPVMSSSGKGQSVIRTPEQIEAAWQY
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCCEECCHHHHHHHHHH
AQQGGRSGAGRVIVEGFVDFDYEITLLTVRAVDGVHFCAPIGHRQEDGDYRESWQPQVMS
HHHCCCCCCCEEEEEEEECCCEEEEEEEEEECCCEEEECCCCCCCCCCCHHHCCCCCHHC
ENAIKAAEYVAEQVVNALGGYGLFGVELFVKGDKVIFNEVSPRPHDTGMVTLISQELSEF
CHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEHHHHHHHHH
ALHVRAFTGLPIGQIVQYGPSASAAILGQGQSQNIQFNGLDDALSIPHTQVRLFGKPDIA
HHHHHHHCCCCHHHHHHCCCCCCEEEEECCCCCCEEECCCCHHHCCCCEEEEEECCCCCC
GRRRLGVALSRGKTTQEATDRAIECAKAVKIHY
CCHHHCEEEECCCCHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure 
SMFGTATRDNATRVLLLGSGELGKEVAIECQRLGLEVIACDRYADAPAMQVAHRSHVFD
CCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHH
MLDADALQQVIDLEKPHFVVPEIEAIATSKLVELEAQGLNVVPTANATRLTMNREGIRRL
HHCHHHHHHHHCCCCCCEECCCHHHHHHHHHHEEECCCCEEEECCCCEEEEECHHHHHHH
AAEELGLPTSAYQFADSYESFAAAVETIGLPCVCKPVMSSSGKGQSVIRTPEQIEAAWQY
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCCEECCHHHHHHHHHH
AQQGGRSGAGRVIVEGFVDFDYEITLLTVRAVDGVHFCAPIGHRQEDGDYRESWQPQVMS
HHHCCCCCCCEEEEEEEECCCEEEEEEEEEECCCEEEECCCCCCCCCCCHHHCCCCCHHC
ENAIKAAEYVAEQVVNALGGYGLFGVELFVKGDKVIFNEVSPRPHDTGMVTLISQELSEF
CHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEHHHHHHHHH
ALHVRAFTGLPIGQIVQYGPSASAAILGQGQSQNIQFNGLDDALSIPHTQVRLFGKPDIA
HHHHHHHCCCCHHHHHHCCCCCCEEEEECCCCCCEEECCCCHHHCCCCEEEEEECCCCCC
GRRRLGVALSRGKTTQEATDRAIECAKAVKIHY
CCHHHCEEEECCCCHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA