Click here to switch to the map view.

The map label for this gene is pheT

Identifier: 227081392

GI number: 227081392

Start: 1254889

End: 1257276

Strand: Direct

Name: pheT

Synonym: VCM66_1175

Alternate gene names: 227081392

Gene position: 1254889-1257276 (Clockwise)

Preceding gene: 227081391

Following gene: 227081393

Centisome position: 43.38

GC content: 52.18

Gene sequence:

>2388_bases
ATGAAATTCAGCGAATCATGGCTTCGTGAGTGGGTTAACCCGGCAATTACCACTGACGAATTAACTCACCAAATTACAAT
GGCGGGTCTGGAAGTGGATGACGTACTGGCCGTAGCTGGTGTGTTCGACGGCGTAAAAGTGGGTCACGTGGTTGAGTGTG
CTCAACATCCAGATGCTGACAAACTGCGCGTCACCAAAGTCGATGTAGGCGAAGAAGAGCTTCTGGACATCGTGTGTGGT
GCAGCCAACTGTCGTCAAGGTCTGAAAGTTGCGGTTGCAACCGTAGGCGCGACGCTGCCAGGCGATTTCAAGATTAAAAA
AGCTAAACTGCGTGGCCAACCGTCACACGGCATGCTGTGTTCTTTCTCTGAGCTGGGTATCGATGTTGAATCGAACGGCA
TCATGGAACTGGCTGAAAACGCACCGATTGGTATGGATTTCCGCGATTTTCTGTCACTGAACGATGTGACTATCGATGTG
GATTTGACGTCTAACCGCGCGGACTGTTTCAGCATCCGTGGTCTGGCTCGTGAAGTGGGCGTATTGAACCGTGCGGACGT
GACTGCTCCTGCGGTGAATGCGATTGTAGCAACCATCAATGATACGATTTCAATTGACGTGAAAGCGCCAGCGGCGTGTC
CGCGTTACCTTGGTCGTATCGTGAAGAACGTGAATGTTCAAGCGCAAACGCCACTCTGGATGCAAGAAAAACTGCGTCGT
TGTGGTATCCGCTCTATCGATCCGGTGGTTGATATCACTAACTTCGTGATGCTGGAGCAAGGCCAACCAATGCACGCGTT
TGATCTGGCGAAGATTGAAGGCGGAATCGTGGTTCGTCTGGCTGAGCAAGATGAAAAACTGACGCTGCTTGATGGCTCAG
AAGCCAAATTAAACGCAGATACGCTAGTGATTGCTGACCAACAAAAAGCGCTGGCGATTGCTGGTGTATTCGGTGGCGAG
CATTCTGGCGTCAGCACTGACACCAAAGATGTATTACTTGAGTGTGCGTTCTTCGCACCAGACCACATTCGTGGTCGTGC
ACGTAGCTACGGCCTACACACCGATTCTTCAATGCGTTTTGAACGTGGTGTGGATTATGCTTTGCAACACGCCGCGATGG
AGCGTGCAACGCAACTGCTGGTTGAGATCTGTGGTGGTGACGTTGCGCCAGTGGTAGCTGCAGAATCTGCGGCCGATTTA
CCAAAACCAAACCAAGTTGCGCTGCGTCGTAGCAAGCTCGACAAACTGCTGGGTCATGCGATTCCGGATGCGGACGTCGT
GGAGATTCTAGAGCGTCTAGGCATGCAGGTCGAGACCACCGCAGAAGGCTGGCAAGCGACCGCGCCAACATGGCGTTTTG
ATATTGCTATCGAGCAAGATCTGGTGGAAGAAGTTGGCCGTATCTATGGTTACAACAACATTCCTAATCAAGCGCCAGTG
GCCGCGTTGAACATGAACCTGCACAACGAAGCGAAATTACCGCTGAAGCGTGTACGCGATCTGCTGGTTGACCGTGGTTA
CCAAGAAGCGATCACCTATAGCTTCGTTGAGCCTGAGCAACAGAAACTGGTTGTACCGGGTGTTGATGCGCTGATCCTGC
CAAACCCAATTTCTGCTGAAATGTCAGCGATGCGTTTGAGCTTGATCCAAGGTCTGCTGAACACTGTGGTTCACAACCAG
AAACGTCAGCAACCTCGTGTTCGTTTGTTTGAATACGGTTTGCGCTTCATTCCTGATGCGGCGGCAGAAAATGGCATGCG
CCAAGAGCCTATGCTGGCGGGTGTGATCTCTGGCGCTCGTGGTGAAGAGCACTGGAATATGGAAACGGCAACCGTTGATT
TCTTCGATATGAAGGGCGATCTGGAAGCAGTTCTTGAACTGACCGCGAAAGGCAAAGCCTATAGCTTCGCCGCGACTAAG
CACCCAGCGCTGCATCCAGGTCAAGCTGCAGCGATTATGGTGGATGGCAAAGCGATCGGTGTTATTGGTACGGTTCACCC
AGAACTTGAGCGTAAGTTTGGCCTCAATGGTCGCACTATTGTGTTCGAGATCGAGTGGAATGCGATCAACACACGCGTGA
TCCCAGAAGCCGCTGCGATTTCTAAGTTCCCAGCCAACCGTCGTGATATCGCGTTGGTGGTCGATGGCAACATCGCTTCT
GGCGACATCGTTGAAGCTTGCCGTGTGGCGGGTGGTGAGCTACTGAAAGACGCAAAACTGTTTGACGTTTATGTCGGTAA
AGGCGTGGAAGAAGGCAAGAAGAGCTTGGCGATTGCGCTGACGTTGCAATCGGTTGAACGCACACTTGAAGAAGCGGATA
TCGCAGCAGCGGTAGAGGCGATTGTGCAAGCCGTATCTGCCCAATTCGGCGCGGCTCTGCGCGACTAA

Upstream 100 bases:

>100_bases
TGCTCCGCTATGGCGTGAATGACCTGCGTGCATTCTTCGAAAACGATCTGCGTTTCCTAAAACAGTTCAAATAATTCAGG
GTTCCCAAGAGGTATATACA

Downstream 100 bases:

>100_bases
GCTTAGGTCTATCTTCGCTTTAAATCAGATGAAAACAGGCTCTTCGGAGCCTGTTTTTTTATCGTGATAATCTGGAACTG
AGCCAACAACTCTCTGATTT

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS

Number of amino acids: Translated: 795; Mature: 795

Protein sequence:

>795_residues
MKFSESWLREWVNPAITTDELTHQITMAGLEVDDVLAVAGVFDGVKVGHVVECAQHPDADKLRVTKVDVGEEELLDIVCG
AANCRQGLKVAVATVGATLPGDFKIKKAKLRGQPSHGMLCSFSELGIDVESNGIMELAENAPIGMDFRDFLSLNDVTIDV
DLTSNRADCFSIRGLAREVGVLNRADVTAPAVNAIVATINDTISIDVKAPAACPRYLGRIVKNVNVQAQTPLWMQEKLRR
CGIRSIDPVVDITNFVMLEQGQPMHAFDLAKIEGGIVVRLAEQDEKLTLLDGSEAKLNADTLVIADQQKALAIAGVFGGE
HSGVSTDTKDVLLECAFFAPDHIRGRARSYGLHTDSSMRFERGVDYALQHAAMERATQLLVEICGGDVAPVVAAESAADL
PKPNQVALRRSKLDKLLGHAIPDADVVEILERLGMQVETTAEGWQATAPTWRFDIAIEQDLVEEVGRIYGYNNIPNQAPV
AALNMNLHNEAKLPLKRVRDLLVDRGYQEAITYSFVEPEQQKLVVPGVDALILPNPISAEMSAMRLSLIQGLLNTVVHNQ
KRQQPRVRLFEYGLRFIPDAAAENGMRQEPMLAGVISGARGEEHWNMETATVDFFDMKGDLEAVLELTAKGKAYSFAATK
HPALHPGQAAAIMVDGKAIGVIGTVHPELERKFGLNGRTIVFEIEWNAINTRVIPEAAAISKFPANRRDIALVVDGNIAS
GDIVEACRVAGGELLKDAKLFDVYVGKGVEEGKKSLAIALTLQSVERTLEEADIAAAVEAIVQAVSAQFGAALRD

Sequences:

>Translated_795_residues
MKFSESWLREWVNPAITTDELTHQITMAGLEVDDVLAVAGVFDGVKVGHVVECAQHPDADKLRVTKVDVGEEELLDIVCG
AANCRQGLKVAVATVGATLPGDFKIKKAKLRGQPSHGMLCSFSELGIDVESNGIMELAENAPIGMDFRDFLSLNDVTIDV
DLTSNRADCFSIRGLAREVGVLNRADVTAPAVNAIVATINDTISIDVKAPAACPRYLGRIVKNVNVQAQTPLWMQEKLRR
CGIRSIDPVVDITNFVMLEQGQPMHAFDLAKIEGGIVVRLAEQDEKLTLLDGSEAKLNADTLVIADQQKALAIAGVFGGE
HSGVSTDTKDVLLECAFFAPDHIRGRARSYGLHTDSSMRFERGVDYALQHAAMERATQLLVEICGGDVAPVVAAESAADL
PKPNQVALRRSKLDKLLGHAIPDADVVEILERLGMQVETTAEGWQATAPTWRFDIAIEQDLVEEVGRIYGYNNIPNQAPV
AALNMNLHNEAKLPLKRVRDLLVDRGYQEAITYSFVEPEQQKLVVPGVDALILPNPISAEMSAMRLSLIQGLLNTVVHNQ
KRQQPRVRLFEYGLRFIPDAAAENGMRQEPMLAGVISGARGEEHWNMETATVDFFDMKGDLEAVLELTAKGKAYSFAATK
HPALHPGQAAAIMVDGKAIGVIGTVHPELERKFGLNGRTIVFEIEWNAINTRVIPEAAAISKFPANRRDIALVVDGNIAS
GDIVEACRVAGGELLKDAKLFDVYVGKGVEEGKKSLAIALTLQSVERTLEEADIAAAVEAIVQAVSAQFGAALRD
>Mature_795_residues
MKFSESWLREWVNPAITTDELTHQITMAGLEVDDVLAVAGVFDGVKVGHVVECAQHPDADKLRVTKVDVGEEELLDIVCG
AANCRQGLKVAVATVGATLPGDFKIKKAKLRGQPSHGMLCSFSELGIDVESNGIMELAENAPIGMDFRDFLSLNDVTIDV
DLTSNRADCFSIRGLAREVGVLNRADVTAPAVNAIVATINDTISIDVKAPAACPRYLGRIVKNVNVQAQTPLWMQEKLRR
CGIRSIDPVVDITNFVMLEQGQPMHAFDLAKIEGGIVVRLAEQDEKLTLLDGSEAKLNADTLVIADQQKALAIAGVFGGE
HSGVSTDTKDVLLECAFFAPDHIRGRARSYGLHTDSSMRFERGVDYALQHAAMERATQLLVEICGGDVAPVVAAESAADL
PKPNQVALRRSKLDKLLGHAIPDADVVEILERLGMQVETTAEGWQATAPTWRFDIAIEQDLVEEVGRIYGYNNIPNQAPV
AALNMNLHNEAKLPLKRVRDLLVDRGYQEAITYSFVEPEQQKLVVPGVDALILPNPISAEMSAMRLSLIQGLLNTVVHNQ
KRQQPRVRLFEYGLRFIPDAAAENGMRQEPMLAGVISGARGEEHWNMETATVDFFDMKGDLEAVLELTAKGKAYSFAATK
HPALHPGQAAAIMVDGKAIGVIGTVHPELERKFGLNGRTIVFEIEWNAINTRVIPEAAAISKFPANRRDIALVVDGNIAS
GDIVEACRVAGGELLKDAKLFDVYVGKGVEEGKKSLAIALTLQSVERTLEEADIAAAVEAIVQAVSAQFGAALRD

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI124028525, Length=396, Percent_Identity=24.7474747474747, Blast_Score=97, Evalue=6e-20,
Organism=Escherichia coli, GI1788006, Length=795, Percent_Identity=64.4025157232704, Blast_Score=1038, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17533403, Length=556, Percent_Identity=22.4820143884892, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21355211, Length=391, Percent_Identity=26.0869565217391, Blast_Score=105, Evalue=2e-22,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFB_VIBCH (Q9KSN6)

Other databases:

- EMBL:   AE003852
- PIR:   D82225
- RefSeq:   NP_230865.1
- ProteinModelPortal:   Q9KSN6
- SMR:   Q9KSN6
- GeneID:   2614657
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC1220
- TIGR:   VC_1220
- HOGENOM:   HBG399638
- OMA:   ADKLRVC
- ProtClustDB:   PRK00629
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00283
- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547
- Gene3D:   G3DSA:3.50.40.10
- Gene3D:   G3DSA:3.30.56.20
- Gene3D:   G3DSA:3.30.70.380
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00873
- SMART:   SM00874
- SMART:   SM00896
- TIGRFAMs:   TIGR00472

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind; SSF56037 B3_4; SSF54991 Fdx_AntiC_bd; SSF50249 Nucleic_acid_OB; SSF46955 Putativ_DNA_bind

EC number: =6.1.1.20

Molecular weight: Translated: 86135; Mature: 86135

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS51483 B5; PS51447 FDX_ACB; PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSESWLREWVNPAITTDELTHQITMAGLEVDDVLAVAGVFDGVKVGHVVECAQHPDAD
CCCCHHHHHHHCCCCCCHHHHHHHHEEECCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
KLRVTKVDVGEEELLDIVCGAANCRQGLKVAVATVGATLPGDFKIKKAKLRGQPSHGMLC
CEEEEEECCCHHHHHHHHHCCHHHHCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCEE
SFSELGIDVESNGIMELAENAPIGMDFRDFLSLNDVTIDVDLTSNRADCFSIRGLAREVG
EHHHHCCEECCCCHHHHHCCCCCCCCHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHC
VLNRADVTAPAVNAIVATINDTISIDVKAPAACPRYLGRIVKNVNVQAQTPLWMQEKLRR
CCCCCCCCHHHHHHEEEEECCEEEEEEECCCCCHHHHHHHHHCCCCEECCCHHHHHHHHH
CGIRSIDPVVDITNFVMLEQGQPMHAFDLAKIEGGIVVRLAEQDEKLTLLDGSEAKLNAD
CCCCCCCHHHHHHHEEEECCCCCCCCEEHEEECCCEEEEEECCCCEEEEEECCCCEECCC
TLVIADQQKALAIAGVFGGEHSGVSTDTKDVLLECAFFAPDHIRGRARSYGLHTDSSMRF
EEEEECCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHCCCHHHCCCCCCCCCHH
ERGVDYALQHAAMERATQLLVEICGGDVAPVVAAESAADLPKPNQVALRRSKLDKLLGHA
HHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHEECCCCCCCCCCHHHHHHHHHHHHHHHHC
IPDADVVEILERLGMQVETTAEGWQATAPTWRFDIAIEQDLVEEVGRIYGYNNIPNQAPV
CCCHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCCE
AALNMNLHNEAKLPLKRVRDLLVDRGYQEAITYSFVEPEQQKLVVPGVDALILPNPISAE
EEEECCCCCCCCCCHHHHHHHHHCCCHHHHHEEEECCCCCCEEEECCCCEEEECCCCCHH
MSAMRLSLIQGLLNTVVHNQKRQQPRVRLFEYGLRFIPDAAAENGMRQEPMLAGVISGAR
HHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHCCCC
GEEHWNMETATVDFFDMKGDLEAVLELTAKGKAYSFAATKHPALHPGQAAAIMVDGKAIG
CCCCCCCEEEEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEECCCEEE
VIGTVHPELERKFGLNGRTIVFEIEWNAINTRVIPEAAAISKFPANRRDIALVVDGNIAS
EEEECCHHHHHHHCCCCCEEEEEEEECEECCEECCCHHHHHCCCCCCCCEEEEEECCCCC
GDIVEACRVAGGELLKDAKLFDVYVGKGVEEGKKSLAIALTLQSVERTLEEADIAAAVEA
CHHHHHHHHCCHHHHHCCCEEEEEECCCHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHH
IVQAVSAQFGAALRD
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFSESWLREWVNPAITTDELTHQITMAGLEVDDVLAVAGVFDGVKVGHVVECAQHPDAD
CCCCHHHHHHHCCCCCCHHHHHHHHEEECCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
KLRVTKVDVGEEELLDIVCGAANCRQGLKVAVATVGATLPGDFKIKKAKLRGQPSHGMLC
CEEEEEECCCHHHHHHHHHCCHHHHCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCEE
SFSELGIDVESNGIMELAENAPIGMDFRDFLSLNDVTIDVDLTSNRADCFSIRGLAREVG
EHHHHCCEECCCCHHHHHCCCCCCCCHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHC
VLNRADVTAPAVNAIVATINDTISIDVKAPAACPRYLGRIVKNVNVQAQTPLWMQEKLRR
CCCCCCCCHHHHHHEEEEECCEEEEEEECCCCCHHHHHHHHHCCCCEECCCHHHHHHHHH
CGIRSIDPVVDITNFVMLEQGQPMHAFDLAKIEGGIVVRLAEQDEKLTLLDGSEAKLNAD
CCCCCCCHHHHHHHEEEECCCCCCCCEEHEEECCCEEEEEECCCCEEEEEECCCCEECCC
TLVIADQQKALAIAGVFGGEHSGVSTDTKDVLLECAFFAPDHIRGRARSYGLHTDSSMRF
EEEEECCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHCCCHHHCCCCCCCCCHH
ERGVDYALQHAAMERATQLLVEICGGDVAPVVAAESAADLPKPNQVALRRSKLDKLLGHA
HHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHEECCCCCCCCCCHHHHHHHHHHHHHHHHC
IPDADVVEILERLGMQVETTAEGWQATAPTWRFDIAIEQDLVEEVGRIYGYNNIPNQAPV
CCCHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCCE
AALNMNLHNEAKLPLKRVRDLLVDRGYQEAITYSFVEPEQQKLVVPGVDALILPNPISAE
EEEECCCCCCCCCCHHHHHHHHHCCCHHHHHEEEECCCCCCEEEECCCCEEEECCCCCHH
MSAMRLSLIQGLLNTVVHNQKRQQPRVRLFEYGLRFIPDAAAENGMRQEPMLAGVISGAR
HHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHCCCC
GEEHWNMETATVDFFDMKGDLEAVLELTAKGKAYSFAATKHPALHPGQAAAIMVDGKAIG
CCCCCCCEEEEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEECCCEEE
VIGTVHPELERKFGLNGRTIVFEIEWNAINTRVIPEAAAISKFPANRRDIALVVDGNIAS
EEEECCHHHHHHHCCCCCEEEEEEEECEECCEECCCHHHHHCCCCCCCCEEEEEECCCCC
GDIVEACRVAGGELLKDAKLFDVYVGKGVEEGKKSLAIALTLQSVERTLEEADIAAAVEA
CHHHHHHHHCCHHHHHCCCEEEEEECCCHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHH
IVQAVSAQFGAALRD
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301