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The map label for this gene is hutU

Identifier: 227081375

GI number: 227081375

Start: 1235277

End: 1236974

Strand: Reverse

Name: hutU

Synonym: VCM66_1158

Alternate gene names: 227081375

Gene position: 1236974-1235277 (Counterclockwise)

Preceding gene: 227081376

Following gene: 227081374

Centisome position: 42.76

GC content: 53.89

Gene sequence:

>1698_bases
ATGACACAGTCATCTGCACAAGGAACAAGACTGGACACTCAGCGCACCATTCGCGCCCCTCGTGGCACACAGCTGCGCGC
CAAATCTTGGCTCACTGAAGCCCCACTTCGCATGCTGATGAATAACCTCGACCCCGATGTGGCCGAACACCCTCATGCAC
TGGTGGTGTATGGTGGCATTGGCCGCGCGGCGCGTAACTGGGAGTGCTTTGACAAAATCGTCGAAGTGCTGGAGCGCCTC
GAAGATGACCAGACTTTACTGGTGCAATCGGGTAAACCGGTGGGGGTTTTCCCCACCCACAAAAATGCGCCACGCGTGTT
GATTGCCAACTCCAACTTAGTACCACACTGGGCAAACTGGGAGCATTTCAACGAGCTCGATAAACAAGGCTTGATGATGT
ACGGCCAGATGACGGCGGGATCTTGGATTTACATTGGCTCACAAGGCATAGTGCAAGGCACTTACGAAACCTTTGTCGCG
GTTGCGAAAAAGCATTTCAATGGCGATGCCAAAGGCCGTTGGGTTTTGACTGGCGGATTGGGCGGCATGGGCGGCGCGCA
ACCTTTAGCGGCGACGATGGCAGGATTCTCGATGATTGCGGTGGAATGTGATGAATCGCGCATCGACTATCGTCTGCGCA
CCGGTTATGTCGACAAAAAAGCCAACACGCTTGATGAAGCGCTGGCGATGATCGCCGATACCGATCGCCCAATTTCTGTC
GGCTTACTGGGTAATGCCGCTGACATCTTCCCCGAATTAGTCAAACGCAACATCACCCCTGATGTGGTGACAGATCAAAC
GTCGGCACACGATCCACTCAATGGCTATTTGCCACTCGGCTGGAGCATGGAAAAAGCTGCACAGATGCGTCAACAAAATG
AAGCTGAAGTCGTCAAAGCTGCCAAAGCTTCGATGGCGATCCAAGTGCGCGCCATGCTTGATTTGCAAACTCGCGGCGCT
GCGACGCTGGACTATGGCAATAACATTCGCCAAATGGCGCTGGAAGAAGGTGTTGCCAATGCGTTCGACTTCCCCGGTTT
TGTGCCGGCCTATATTCGTCCACTCTTCTGTGAAGGGATAGGTCCCTTCCGTTGGGCGGCACTCTCTGGCGATCCGGAAG
ACATTTACAAAACCGATCAAAAAGTCAAAGAGCTGATCCCTGACAACCCACATCTGCATAACTGGCTGGATATGGCGCGT
GAGCGAATCCACTTCCAAGGTTTACCTGCCCGTATTTGCTGGGTCGGTTTAAAAGATCGCGCTCGCTTAGGCTTAGCTTT
TAACGAAATGGTGAAAAATGGCGAGCTCAAAGCGCCAATCGTGATTGGTCGTGATCACCTCGATTCAGGCTCAGTCGCCA
GCCCGAACCGCGAAACCGAAGGCATGTTGGATGGTTCAGATGCAGTCTCTGATTGGCCACTGCTCAATGCCCTACTCAAC
ACCGCAGGCGGAGCCACTTGGGTTTCTCTGCACCACGGTGGTGGCGTTGGTATGGGGTTCTCACAGCATTCCGGTATGGT
GATTTGCTGCGATGGCAGTGATGATGCCGCCGAACGTATCGCTCGTGTACTGCACAATGACCCAGCCACAGGCGTAATGC
GCCACGCTGATGCGGGCTATGAGATTGCCAAACGCTGCGCGCAGCAACAAAAACTCGACTTACCTATGCTCAACGCTGAG
CTGGCCAAACTCAAGTGA

Upstream 100 bases:

>100_bases
CACCGCGCGCTTAGCCGCTCGTTTGTGTTGGGACATTGCTAACGCCATGGCCGAACAAGTGCAATCCATCCGTCACCCGT
GAATCAAAAGGAGTCTCAAC

Downstream 100 bases:

>100_bases
GGAAAGAAACCATGTTGCACCTGATGATCAAACCCGGCCAACTCAGCTTAAAACAGTTGCGTCAAGTGAGCCGCTCACCC
GTGGTGTTATCCCTCGATCC

Product: urocanate hydratase

Products: NA

Alternate protein names: Urocanase; Imidazolonepropionate hydrolase

Number of amino acids: Translated: 565; Mature: 564

Protein sequence:

>565_residues
MTQSSAQGTRLDTQRTIRAPRGTQLRAKSWLTEAPLRMLMNNLDPDVAEHPHALVVYGGIGRAARNWECFDKIVEVLERL
EDDQTLLVQSGKPVGVFPTHKNAPRVLIANSNLVPHWANWEHFNELDKQGLMMYGQMTAGSWIYIGSQGIVQGTYETFVA
VAKKHFNGDAKGRWVLTGGLGGMGGAQPLAATMAGFSMIAVECDESRIDYRLRTGYVDKKANTLDEALAMIADTDRPISV
GLLGNAADIFPELVKRNITPDVVTDQTSAHDPLNGYLPLGWSMEKAAQMRQQNEAEVVKAAKASMAIQVRAMLDLQTRGA
ATLDYGNNIRQMALEEGVANAFDFPGFVPAYIRPLFCEGIGPFRWAALSGDPEDIYKTDQKVKELIPDNPHLHNWLDMAR
ERIHFQGLPARICWVGLKDRARLGLAFNEMVKNGELKAPIVIGRDHLDSGSVASPNRETEGMLDGSDAVSDWPLLNALLN
TAGGATWVSLHHGGGVGMGFSQHSGMVICCDGSDDAAERIARVLHNDPATGVMRHADAGYEIAKRCAQQQKLDLPMLNAE
LAKLK

Sequences:

>Translated_565_residues
MTQSSAQGTRLDTQRTIRAPRGTQLRAKSWLTEAPLRMLMNNLDPDVAEHPHALVVYGGIGRAARNWECFDKIVEVLERL
EDDQTLLVQSGKPVGVFPTHKNAPRVLIANSNLVPHWANWEHFNELDKQGLMMYGQMTAGSWIYIGSQGIVQGTYETFVA
VAKKHFNGDAKGRWVLTGGLGGMGGAQPLAATMAGFSMIAVECDESRIDYRLRTGYVDKKANTLDEALAMIADTDRPISV
GLLGNAADIFPELVKRNITPDVVTDQTSAHDPLNGYLPLGWSMEKAAQMRQQNEAEVVKAAKASMAIQVRAMLDLQTRGA
ATLDYGNNIRQMALEEGVANAFDFPGFVPAYIRPLFCEGIGPFRWAALSGDPEDIYKTDQKVKELIPDNPHLHNWLDMAR
ERIHFQGLPARICWVGLKDRARLGLAFNEMVKNGELKAPIVIGRDHLDSGSVASPNRETEGMLDGSDAVSDWPLLNALLN
TAGGATWVSLHHGGGVGMGFSQHSGMVICCDGSDDAAERIARVLHNDPATGVMRHADAGYEIAKRCAQQQKLDLPMLNAE
LAKLK
>Mature_564_residues
TQSSAQGTRLDTQRTIRAPRGTQLRAKSWLTEAPLRMLMNNLDPDVAEHPHALVVYGGIGRAARNWECFDKIVEVLERLE
DDQTLLVQSGKPVGVFPTHKNAPRVLIANSNLVPHWANWEHFNELDKQGLMMYGQMTAGSWIYIGSQGIVQGTYETFVAV
AKKHFNGDAKGRWVLTGGLGGMGGAQPLAATMAGFSMIAVECDESRIDYRLRTGYVDKKANTLDEALAMIADTDRPISVG
LLGNAADIFPELVKRNITPDVVTDQTSAHDPLNGYLPLGWSMEKAAQMRQQNEAEVVKAAKASMAIQVRAMLDLQTRGAA
TLDYGNNIRQMALEEGVANAFDFPGFVPAYIRPLFCEGIGPFRWAALSGDPEDIYKTDQKVKELIPDNPHLHNWLDMARE
RIHFQGLPARICWVGLKDRARLGLAFNEMVKNGELKAPIVIGRDHLDSGSVASPNRETEGMLDGSDAVSDWPLLNALLNT
AGGATWVSLHHGGGVGMGFSQHSGMVICCDGSDDAAERIARVLHNDPATGVMRHADAGYEIAKRCAQQQKLDLPMLNAEL
AKLK

Specific function: Unknown

COG id: COG2987

COG function: function code E; Urocanate hydratase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the urocanase family

Homologues:

Organism=Homo sapiens, GI21389467, Length=569, Percent_Identity=33.9191564147627, Blast_Score=305, Evalue=1e-82,
Organism=Homo sapiens, GI260306182, Length=213, Percent_Identity=38.4976525821596, Blast_Score=162, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI71997891, Length=587, Percent_Identity=33.9011925042589, Blast_Score=316, Evalue=3e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HUTU_VIBC3 (A5F1X6)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F1X6
- SMR:   A5F1X6
- STRING:   A5F1X6
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0823
- eggNOG:   COG2987
- HOGENOM:   HBG305285
- OMA:   IRQMAFE
- ProtClustDB:   PRK05414
- BioCyc:   VCHO345073:VC0395_A0823-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00577
- InterPro:   IPR000193
- PIRSF:   PIRSF001423
- TIGRFAMs:   TIGR01228

Pfam domain/function: PF01175 Urocanase; SSF111326 Urocanase

EC number: =4.2.1.49

Molecular weight: Translated: 61825; Mature: 61694

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS01233 UROCANASE

Important sites: ACT_SITE 413-413

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSSAQGTRLDTQRTIRAPRGTQLRAKSWLTEAPLRMLMNNLDPDVAEHPHALVVYGGI
CCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEEECCC
GRAARNWECFDKIVEVLERLEDDQTLLVQSGKPVGVFPTHKNAPRVLIANSNLVPHWANW
CCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCCEEEEECCCCCCCCCCC
EHFNELDKQGLMMYGQMTAGSWIYIGSQGIVQGTYETFVAVAKKHFNGDAKGRWVLTGGL
HHHHHHHHCCEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCC
GGMGGAQPLAATMAGFSMIAVECDESRIDYRLRTGYVDKKANTLDEALAMIADTDRPISV
CCCCCCCHHHHHHCCCEEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEE
GLLGNAADIFPELVKRNITPDVVTDQTSAHDPLNGYLPLGWSMEKAAQMRQQNEAEVVKA
EEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHCCHHHHHHH
AKASMAIQVRAMLDLQTRGAATLDYGNNIRQMALEEGVANAFDFPGFVPAYIRPLFCEGI
HHHHHEEEEEEEEEHHCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
GPFRWAALSGDPEDIYKTDQKVKELIPDNPHLHNWLDMARERIHFQGLPARICWVGLKDR
CCCEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCC
ARLGLAFNEMVKNGELKAPIVIGRDHLDSGSVASPNRETEGMLDGSDAVSDWPLLNALLN
HHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
TAGGATWVSLHHGGGVGMGFSQHSGMVICCDGSDDAAERIARVLHNDPATGVMRHADAGY
CCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHCCCCH
EIAKRCAQQQKLDLPMLNAELAKLK
HHHHHHHHHHCCCCCCCCCHHHHCC
>Mature Secondary Structure 
TQSSAQGTRLDTQRTIRAPRGTQLRAKSWLTEAPLRMLMNNLDPDVAEHPHALVVYGGI
CCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEEECCC
GRAARNWECFDKIVEVLERLEDDQTLLVQSGKPVGVFPTHKNAPRVLIANSNLVPHWANW
CCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCCEEEEECCCCCCCCCCC
EHFNELDKQGLMMYGQMTAGSWIYIGSQGIVQGTYETFVAVAKKHFNGDAKGRWVLTGGL
HHHHHHHHCCEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCC
GGMGGAQPLAATMAGFSMIAVECDESRIDYRLRTGYVDKKANTLDEALAMIADTDRPISV
CCCCCCCHHHHHHCCCEEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEE
GLLGNAADIFPELVKRNITPDVVTDQTSAHDPLNGYLPLGWSMEKAAQMRQQNEAEVVKA
EEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHCCHHHHHHH
AKASMAIQVRAMLDLQTRGAATLDYGNNIRQMALEEGVANAFDFPGFVPAYIRPLFCEGI
HHHHHEEEEEEEEEHHCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
GPFRWAALSGDPEDIYKTDQKVKELIPDNPHLHNWLDMARERIHFQGLPARICWVGLKDR
CCCEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCC
ARLGLAFNEMVKNGELKAPIVIGRDHLDSGSVASPNRETEGMLDGSDAVSDWPLLNALLN
HHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
TAGGATWVSLHHGGGVGMGFSQHSGMVICCDGSDDAAERIARVLHNDPATGVMRHADAGY
CCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHCCCCH
EIAKRCAQQQKLDLPMLNAELAKLK
HHHHHHHHHHCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA