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The map label for this gene is sfcA [H]

Identifier: 227081360

GI number: 227081360

Start: 1219169

End: 1220935

Strand: Reverse

Name: sfcA [H]

Synonym: VCM66_1143

Alternate gene names: 227081360

Gene position: 1220935-1219169 (Counterclockwise)

Preceding gene: 227081362

Following gene: 227081359

Centisome position: 42.21

GC content: 50.37

Gene sequence:

>1767_bases
TTGTCTATTTACAATCCAACCAGCAAGTCGGTATCATTTTCGCGTTTCACTTCTACCCTACCAATCAATAGGCCGAACAT
GAATAACGATAAACGTCCGCTGTATATTTCCTACGCAGGCCCAGCTCTTCTCAGTACTCCTCTGCTCAACAAAGGTAGTG
CCTTTAGTGCGGAAGAACGTGCTTCTTTTAACCTAGAAGGTTTACTGCCCGAAGCGACGGAAACCATTCAAGAACAAGTG
GTACGCGCATACCAACAGTACCGTGGTTTTGAAAGCGACATGGATAAGCATATTTATCTGCGCAACATTCAAGACACCAA
CGAGACCCTATTCTACCGCTTGGTGCAGAACCATATTTCCGAGATGATGCCAATCATCTATACCCCGACAGTGGGTGCGG
CGTGTGAGAACTTCTCCAACATTTATCGCCGTGGCCGTGGCTTGTTTATCTCTTACGCTAACCGTGATCGTATTGATGAC
TTGTTGAACAATGCCGCCAACCACAACGTCAAAGTGATTGTGGTGACCGATGGCGAGCGTATTCTCGGCCTTGGCGACCA
AGGTATCGGCGGTATGGGGATTCCTATCGGTAAACTGTCACTTTACACCGCTTGTGGTGGCATTAGCCCAGCGTATACGC
TTCCTGTGGTTCTGGATGTGGGTACGAATAACCCGCAACGTCTTGCTGACCCTATGTACATGGGCTGGCGTCATCCACGT
ATCACTGGACCTGACTACGACAACTTTGTTGAAGAGTTTATGCAAGCCGTGCAACGTCGCTGGCCAGATGCATTGATCCA
GTTTGAAGACTTTGCCCAAAAGAACGCCATGCCACTGCTTGAGCGTTACAAAAATCGCGTGTGCTGTTTTAACGACGACA
TTCAGGGCACAGCGGCGGTCACTGTCGGCTCACTGCTTGCTGCCTGTAAAGCGGCGGGTAGCCAGCTTTCACAACAACGT
ATTACCTTCTTGGGCGCGGGCTCTGCAGGCTGCGGTATCGCGGAAGCGATCATTGCGCAAATGGTGTCTGAAGGCATCAG
CGATGAGCAAGCGCGCTCACAAGTTTACATGGTGGATCGCTGGGGCCTGCTCGAAGAAGGCATGCCAAATTTGCTCGACT
TCCAACAGCGCCTAGTGCAGAAAAAAGCCAACACGCAACATTGGACAACGGAAAACAACGGCTACTCGCTGCATGATGTG
ATCCGCAATGCCAAACCGACTGTACTGGTTGGTGTGTCTGGTGCGCCCGGTCTGTTTAGTGAAGAGATCATCAAAGAGAT
GCATCAGCACTGTCCACGCCCTATTGTCTTCCCGCTATCGAACCCAACAAGCCGTGTTGAAGCTCTGCCGAGCGATATTA
TCCGTTGGACTAACGGTGAGGCTTTGGTTGCAACAGGTAGCCCGTTTGATCCTGTGCTGCATGAAGGCAAAACTTACCCA
ATTGTGCAATGTAACAACAGCTACATTTTCCCGGGTATCGGTTTAGGAGTTTTGGCAGCAAACGCACGCCGCGTTACCGA
CGAAATGCTGATGGAGTCGAGCCGTGCACTGGCAAGTTGCTCACCGCTTGCCATCAATGGTCATGGACCATTATTGCCGC
CTCTGGAATCTATCCACTCTGTTTCGAAGAAGATTGCTTTCGCAGTAGCGAAAAAAGCGATTGAACAGGGTGTGGCTCCA
GAAGTCACCGATGAAGCGCTGGAAGCCTCAATTGAACAACATTTCTGGCAACCAGTTTACCGCCGTTATAAGCGCACTGC
GTTTTAA

Upstream 100 bases:

>100_bases
CTTAACTCGTTGCACATAAATCCCCCACCCTAAACCGATAAACCTGTTTTAAACACTGTGCTTGCGCACAGAAGCAGGAT
AATCAACAGAGGCAAGCCGT

Downstream 100 bases:

>100_bases
TTTATGCGGAGAAGCCCCTGCAATCAGGGGCTTTCTAATCTCATGCAACTATTTACTTATCTTGCTCCTGCAGGTCAGGC
TCTACTCCCCTACCTGAGCG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 588; Mature: 587

Protein sequence:

>588_residues
MSIYNPTSKSVSFSRFTSTLPINRPNMNNDKRPLYISYAGPALLSTPLLNKGSAFSAEERASFNLEGLLPEATETIQEQV
VRAYQQYRGFESDMDKHIYLRNIQDTNETLFYRLVQNHISEMMPIIYTPTVGAACENFSNIYRRGRGLFISYANRDRIDD
LLNNAANHNVKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNPQRLADPMYMGWRHPR
ITGPDYDNFVEEFMQAVQRRWPDALIQFEDFAQKNAMPLLERYKNRVCCFNDDIQGTAAVTVGSLLAACKAAGSQLSQQR
ITFLGAGSAGCGIAEAIIAQMVSEGISDEQARSQVYMVDRWGLLEEGMPNLLDFQQRLVQKKANTQHWTTENNGYSLHDV
IRNAKPTVLVGVSGAPGLFSEEIIKEMHQHCPRPIVFPLSNPTSRVEALPSDIIRWTNGEALVATGSPFDPVLHEGKTYP
IVQCNNSYIFPGIGLGVLAANARRVTDEMLMESSRALASCSPLAINGHGPLLPPLESIHSVSKKIAFAVAKKAIEQGVAP
EVTDEALEASIEQHFWQPVYRRYKRTAF

Sequences:

>Translated_588_residues
MSIYNPTSKSVSFSRFTSTLPINRPNMNNDKRPLYISYAGPALLSTPLLNKGSAFSAEERASFNLEGLLPEATETIQEQV
VRAYQQYRGFESDMDKHIYLRNIQDTNETLFYRLVQNHISEMMPIIYTPTVGAACENFSNIYRRGRGLFISYANRDRIDD
LLNNAANHNVKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNPQRLADPMYMGWRHPR
ITGPDYDNFVEEFMQAVQRRWPDALIQFEDFAQKNAMPLLERYKNRVCCFNDDIQGTAAVTVGSLLAACKAAGSQLSQQR
ITFLGAGSAGCGIAEAIIAQMVSEGISDEQARSQVYMVDRWGLLEEGMPNLLDFQQRLVQKKANTQHWTTENNGYSLHDV
IRNAKPTVLVGVSGAPGLFSEEIIKEMHQHCPRPIVFPLSNPTSRVEALPSDIIRWTNGEALVATGSPFDPVLHEGKTYP
IVQCNNSYIFPGIGLGVLAANARRVTDEMLMESSRALASCSPLAINGHGPLLPPLESIHSVSKKIAFAVAKKAIEQGVAP
EVTDEALEASIEQHFWQPVYRRYKRTAF
>Mature_587_residues
SIYNPTSKSVSFSRFTSTLPINRPNMNNDKRPLYISYAGPALLSTPLLNKGSAFSAEERASFNLEGLLPEATETIQEQVV
RAYQQYRGFESDMDKHIYLRNIQDTNETLFYRLVQNHISEMMPIIYTPTVGAACENFSNIYRRGRGLFISYANRDRIDDL
LNNAANHNVKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNPQRLADPMYMGWRHPRI
TGPDYDNFVEEFMQAVQRRWPDALIQFEDFAQKNAMPLLERYKNRVCCFNDDIQGTAAVTVGSLLAACKAAGSQLSQQRI
TFLGAGSAGCGIAEAIIAQMVSEGISDEQARSQVYMVDRWGLLEEGMPNLLDFQQRLVQKKANTQHWTTENNGYSLHDVI
RNAKPTVLVGVSGAPGLFSEEIIKEMHQHCPRPIVFPLSNPTSRVEALPSDIIRWTNGEALVATGSPFDPVLHEGKTYPI
VQCNNSYIFPGIGLGVLAANARRVTDEMLMESSRALASCSPLAINGHGPLLPPLESIHSVSKKIAFAVAKKAIEQGVAPE
VTDEALEASIEQHFWQPVYRRYKRTAF

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=522, Percent_Identity=45.4022988505747, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI62420882, Length=562, Percent_Identity=41.6370106761566, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI62420880, Length=562, Percent_Identity=41.6370106761566, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI239049447, Length=562, Percent_Identity=41.6370106761566, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI4505145, Length=554, Percent_Identity=42.057761732852, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI270265879, Length=463, Percent_Identity=44.4924406047516, Blast_Score=395, Evalue=1e-110,
Organism=Escherichia coli, GI87081919, Length=558, Percent_Identity=69.8924731182796, Blast_Score=810, Evalue=0.0,
Organism=Escherichia coli, GI1788806, Length=407, Percent_Identity=25.3071253071253, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17537199, Length=549, Percent_Identity=43.5336976320583, Blast_Score=430, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6322823, Length=559, Percent_Identity=44.0071556350626, Blast_Score=467, Evalue=1e-132,
Organism=Drosophila melanogaster, GI281362672, Length=527, Percent_Identity=43.4535104364326, Blast_Score=437, Evalue=1e-122,
Organism=Drosophila melanogaster, GI281362674, Length=527, Percent_Identity=43.4535104364326, Blast_Score=437, Evalue=1e-122,
Organism=Drosophila melanogaster, GI21356279, Length=527, Percent_Identity=43.4535104364326, Blast_Score=437, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24646386, Length=526, Percent_Identity=41.6349809885932, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24646388, Length=524, Percent_Identity=41.793893129771, Blast_Score=414, Evalue=1e-115,
Organism=Drosophila melanogaster, GI78707232, Length=509, Percent_Identity=37.721021611002, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI281363505, Length=511, Percent_Identity=37.3776908023483, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI78707236, Length=511, Percent_Identity=37.3776908023483, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI78707242, Length=534, Percent_Identity=37.8277153558052, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI78707238, Length=534, Percent_Identity=37.8277153558052, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI281363503, Length=509, Percent_Identity=37.1316306483301, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI78707240, Length=510, Percent_Identity=38.4313725490196, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI19922384, Length=553, Percent_Identity=30.0180831826401, Blast_Score=246, Evalue=3e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 65081; Mature: 64950

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIYNPTSKSVSFSRFTSTLPINRPNMNNDKRPLYISYAGPALLSTPLLNKGSAFSAEER
CCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCEEEEEECCCHHHHCCCCCCCCCCCCHHH
ASFNLEGLLPEATETIQEQVVRAYQQYRGFESDMDKHIYLRNIQDTNETLFYRLVQNHIS
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCEEEEECCCCCHHHHHHHHHHHHHH
EMMPIIYTPTVGAACENFSNIYRRGRGLFISYANRDRIDDLLNNAANHNVKVIVVTDGER
HHCCEEECCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCEEEEEEECCCE
ILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNPQRLADPMYMGWRHPR
EEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHCCHHHHCCCCCC
ITGPDYDNFVEEFMQAVQRRWPDALIQFEDFAQKNAMPLLERYKNRVCCFNDDIQGTAAV
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHCCCEEEECCCCCCHHHH
TVGSLLAACKAAGSQLSQQRITFLGAGSAGCGIAEAIIAQMVSEGISDEQARSQVYMVDR
HHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHCEEEEEEC
WGLLEEGMPNLLDFQQRLVQKKANTQHWTTENNGYSLHDVIRNAKPTVLVGVSGAPGLFS
CCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHCCCCEEEEECCCCCCCHH
EEIIKEMHQHCPRPIVFPLSNPTSRVEALPSDIIRWTNGEALVATGSPFDPVLHEGKTYP
HHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHEECCCCEEEECCCCCCHHHHCCCCCE
IVQCNNSYIFPGIGLGVLAANARRVTDEMLMESSRALASCSPLAINGHGPLLPPLESIHS
EEEECCCEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHH
VSKKIAFAVAKKAIEQGVAPEVTDEALEASIEQHFWQPVYRRYKRTAF
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIYNPTSKSVSFSRFTSTLPINRPNMNNDKRPLYISYAGPALLSTPLLNKGSAFSAEER
CCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCEEEEEECCCHHHHCCCCCCCCCCCCHHH
ASFNLEGLLPEATETIQEQVVRAYQQYRGFESDMDKHIYLRNIQDTNETLFYRLVQNHIS
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCEEEEECCCCCHHHHHHHHHHHHHH
EMMPIIYTPTVGAACENFSNIYRRGRGLFISYANRDRIDDLLNNAANHNVKVIVVTDGER
HHCCEEECCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCEEEEEEECCCE
ILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNPQRLADPMYMGWRHPR
EEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHCCHHHHCCCCCC
ITGPDYDNFVEEFMQAVQRRWPDALIQFEDFAQKNAMPLLERYKNRVCCFNDDIQGTAAV
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHCCCEEEECCCCCCHHHH
TVGSLLAACKAAGSQLSQQRITFLGAGSAGCGIAEAIIAQMVSEGISDEQARSQVYMVDR
HHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHCEEEEEEC
WGLLEEGMPNLLDFQQRLVQKKANTQHWTTENNGYSLHDVIRNAKPTVLVGVSGAPGLFS
CCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHCCCCEEEEECCCCCCCHH
EEIIKEMHQHCPRPIVFPLSNPTSRVEALPSDIIRWTNGEALVATGSPFDPVLHEGKTYP
HHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHEECCCCEEEECCCCCCHHHHCCCCCE
IVQCNNSYIFPGIGLGVLAANARRVTDEMLMESSRALASCSPLAINGHGPLLPPLESIHS
EEEECCCEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHH
VSKKIAFAVAKKAIEQGVAPEVTDEALEASIEQHFWQPVYRRYKRTAF
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA