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The map label for this gene is hisF [H]

Identifier: 227081311

GI number: 227081311

Start: 1164454

End: 1165227

Strand: Direct

Name: hisF [H]

Synonym: VCM66_1094

Alternate gene names: 227081311

Gene position: 1164454-1165227 (Clockwise)

Preceding gene: 227081310

Following gene: 227081312

Centisome position: 40.26

GC content: 51.03

Gene sequence:

>774_bases
ATGTTGGCAAAACGGATAATTCCTTGTCTGGATGTACGTGATGGACAGGTAGTGAAAGGCGTTCAGTTTCGTAACCACGA
AATCATTGGAGACATTGTGCCACTGGCCAAACGTTACGCCGAAGAAGGCGCGGATGAGCTGGTGTTTTACGATATTACCG
CTTCCAGTGATGGCCGCGTTGTCGATAAAAGCTGGGTCGCGCGAGTCGCGGAAGTGATCGACATCCCTTTCTGTGTCGCG
GGCGGGATCAAGTCGGCGCAAGATGCTGCGCGAATCTTGGAGTTCGGTGCAGATAAAGTGTCAATTAACTCGCCTGCACT
GGCGAATCCACAACTGATCACCGATCTTGCGGATCGCTTTGGCGTGCAATGTATCGTGGTTGGCATCGACTCTTATTTTG
ATAAAGAAACAGGTCAATATCAGGTTTATCAGTTTACCGGTGACGAAAGCCGCACCCGTGCCACGCAGTGGCAAACTTGC
GATTGGGTGCAAGAGGTGCAAAAGCGCGGCGCTGGGGAAATTGTACTGAACATGATGAACCAAGATGGTGTGCGCAATGG
TTACGATCTTGAGCAGCTCAACTTAGTGCGCTCGGTGTGCCGAGTTCCGCTGATCGCCTCCGGTGGTGCAGGGGCGATGG
AACATTTCGCGCAAGCTTTTACTCAAGCGAACGTCGATGGTGCTTTAGCCGCTTCGGTGTTTCACAAGCAGATCATCAAT
ATCGGTGAACTCAAGCAGTATCTAAAACAACAAGGAATTGAGGTGAGACGATGA

Upstream 100 bases:

>100_bases
CACTTGCCGATATTGAGGCATTGAAAGGCACAGGCGTTGCGGGTGTGATTGTCGGTCGCGCGCTATTGGATGGCAAATTT
ACCGCGCAGGAGGCATTCGC

Downstream 100 bases:

>100_bases
ATCCAGCAAGCCCATTTGCCACGCTAACCGACCGTATTGATTGGCAGAAAGTCGATGGACTTGTGCCCGCCATTGTGCAA
GATTTTCAGTCCAGCCAAGT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF1; ImGP synthase subunit hisF1; IGPS subunit hisF1 [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRVVDKSWVARVAEVIDIPFCVA
GGIKSAQDAARILEFGADKVSINSPALANPQLITDLADRFGVQCIVVGIDSYFDKETGQYQVYQFTGDESRTRATQWQTC
DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLNLVRSVCRVPLIASGGAGAMEHFAQAFTQANVDGALAASVFHKQIIN
IGELKQYLKQQGIEVRR

Sequences:

>Translated_257_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRVVDKSWVARVAEVIDIPFCVA
GGIKSAQDAARILEFGADKVSINSPALANPQLITDLADRFGVQCIVVGIDSYFDKETGQYQVYQFTGDESRTRATQWQTC
DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLNLVRSVCRVPLIASGGAGAMEHFAQAFTQANVDGALAASVFHKQIIN
IGELKQYLKQQGIEVRR
>Mature_257_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRVVDKSWVARVAEVIDIPFCVA
GGIKSAQDAARILEFGADKVSINSPALANPQLITDLADRFGVQCIVVGIDSYFDKETGQYQVYQFTGDESRTRATQWQTC
DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLNLVRSVCRVPLIASGGAGAMEHFAQAFTQANVDGALAASVFHKQIIN
IGELKQYLKQQGIEVRR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=86.328125, Blast_Score=460, Evalue=1e-131,
Organism=Escherichia coli, GI87082028, Length=219, Percent_Identity=27.8538812785388, Blast_Score=70, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6319725, Length=317, Percent_Identity=31.5457413249211, Blast_Score=135, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 28339; Mature: 28339

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVARVAEVIDIPFCVAGGIKSAQDAARILEFGADKVSINSPALANPQLITDLADRF
ECHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHH
GVQCIVVGIDSYFDKETGQYQVYQFTGDESRTRATQWQTCDWVQEVQKRGAGEIVLNMMN
CCEEEEEECHHHHCCCCCCEEEEEEECCCCHHHHHCCCCHHHHHHHHHCCCCCEEHHHHH
QDGVRNGYDLEQLNLVRSVCRVPLIASGGAGAMEHFAQAFTQANVDGALAASVFHKQIIN
CCCCCCCCCHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
IGELKQYLKQQGIEVRR
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVARVAEVIDIPFCVAGGIKSAQDAARILEFGADKVSINSPALANPQLITDLADRF
ECHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHH
GVQCIVVGIDSYFDKETGQYQVYQFTGDESRTRATQWQTCDWVQEVQKRGAGEIVLNMMN
CCEEEEEECHHHHCCCCCCEEEEEEECCCCHHHHHCCCCHHHHHHHHHCCCCCEEHHHHH
QDGVRNGYDLEQLNLVRSVCRVPLIASGGAGAMEHFAQAFTQANVDGALAASVFHKQIIN
CCCCCCCCCHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
IGELKQYLKQQGIEVRR
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA