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The map label for this gene is ykoW [H]

Identifier: 227081240

GI number: 227081240

Start: 1090671

End: 1092752

Strand: Direct

Name: ykoW [H]

Synonym: VCM66_1022

Alternate gene names: 227081240

Gene position: 1090671-1092752 (Clockwise)

Preceding gene: 227081239

Following gene: 227081241

Centisome position: 37.71

GC content: 45.24

Gene sequence:

>2082_bases
GTGGGGGTATTTACGATAAAATGCGCAGCAAAAAATACAACATACCATTATTCAACGTCTATGCTACTTAACGCTTTTTC
ACGCCGAGTCTTCCTTTGGCTAGGTTGGCTATTGATTTCCACCAGCAGTTTAGCCGCTACATCTACGACGTATAAGGTCG
CCACCGAAGCGGATGACGTGGTGACTCGTGTGCTTTTTGATTCGATTGCTCACCACTTCAACCTTGAAATTGAATACGTC
AACTACCCCAGTTTTAACGATATTCTGGTGGCGATAGAGACTGGCAACGCCGATTTTGCTGCCAACATTACTTACACTGA
TTTGCGTGCTCAACGTTTTGATTTTTCAAGACCAACCAACATCGAGTACACCTATCTCTACAGTTATGGTGGCCTACGTT
TACCCGAGTTGCGCCTCGTGGGTATCCCGAAAGGAACCACCTACGGGACCCTACTAAAAGAACACTATCCCTATATCCAG
CAAGTTGAGTATGAAGGGCATTTAGAAGCGCTCACTTTGCTGGAAAGTGGCCGAGTAGACGGAGTGGTTGATGCGATCAA
TCAGCTCAAACCTATGCTACTGAAAGGGCTTGATGTACAACTCCTTAACGACCAATTACCGATTCAGCCTGTTTCTATTG
TGACGCCTAAAGGCAAACACTCAGCGCTATTGGGCAAGATTGAAAAATACGCGCATTCGGCTCACGTACAACGTTTATTG
CGTGAATCGATCCAAAAGTATCAATTGGACATCCGTAAGCAAGCTCTGCGTCAATCCGTGGTTGAGAGCGGACTCAACGT
GCAGCGTGTATTGCGTGTTAAGCTAGAGAACAACCCGCAATATGCACTTTATCAGCCAGACGGTTCGGTTCGTGGGATCA
GTGCTGATGTTGTGTTTCAGGCCTGTGAGATGCTACTGCTGAAATGCGAATTGGTCAGTAATGGTCAAGAAACATGGGAG
AGCATGTTTGATGATTTACAGGATAAAAGCATCGATATTTTGGCTCCTATAACGGTTTCTCAGCAGCGTAAAAACCTCGC
TTACTTCAGTGAAAGCTACTACCACCCACAAGCGATTTTGGTCAAACGTGAACACTATAAAGACGATGTGTATAGCAATG
TGTCTGAGTTGGTGGCTGAACGTATTGGCGTCATCAAAGACGATTTTTTTGAAGAGCTGTTACAGCAGATGCTGCCGAAC
AAGATCTTGTTCAGCTACGCAAGTCAGGAAGAGAAAGTTCAAGCCTTACTGAATAAAGAGGTGGACTACATAGTGCTCAA
TAGAGCCAATTTTAATCTCTTGCTTCGCGAGTCAACGGAGATGTTACCGATTGTAGAAGACACCATGATTGGCAGTTTCT
ACCAATATGACATTGCGATAGGTTTTGCTAAAAATCCACTTGGTGCAACTCTGGCACCTCTTTTCTCTCGGGCAATTAAA
ATGCTCAATACCGAACAGATCATACATACCTATGATTATCAGCCAAATTGGCGAGCCACATTACTTGCGGAAAAGAAATA
TCAGCGCAGTACTCAATGGCTTTTTGCCATGGCTTTCATCGTTTTGTTTATGGTGGCGTTTTACCTCCATGGCATATCAC
ATACCGATAACCTTACTAAGTTGCGCAATCGTCGCGCTTTGTATAACCGATACCGCCGCGGGTTATCGCCTCGCCTAAGC
TTGGTTTATCTTGACGTGAATACGTTTAAATCAATCAACGATCAGTATGGACATGAAGTGGGTGACAAAGTCCTTAAGCA
GTTGGCTCAGCGCATCGAAGCGGTATGGCGTGGGCGCAGCTATCGGATTGGTGGGGATGAATTTATTTTAATCGGTGAAT
GTTCTGCTAAGCGGCTTGAACATGTGGTTGCGCAATGTGAACGTTTTATGTTTGTGGATGCAGAGCGCGATGTCAGTTTT
GAAGTGAGTGTGGCGATTGGTATTGCTAAGAATCGTGAGCGGACCGAATCACTCAATGAGGTGATGCACCAAGCGGATAT
TGCGATGTATCGCGCTAAGGCGGAATCGACGCAATCGCCATTTCAGGCTGCCAGCAAGGTAAAAGGATTACACATCGTTT
AA

Upstream 100 bases:

>100_bases
AAGCGCACGAATAGTCCATTGAGTGCATCAAATAGCCAGATGAGAGCCTAATAAAGCATCGGTGGCTCTATGAAATTTAT
TACCTAAAGTTGATAGTGGG

Downstream 100 bases:

>100_bases
TCAAACTGGTGAAATGCTGTTCGGTTGATGAGCAATGTGGGGGTAAATCAGGATTATCGCTTGCATTGCCTAAGTCGATG
TTTAATAATCGCACCCGCTT

Product: GGDEF family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 693; Mature: 692

Protein sequence:

>693_residues
MGVFTIKCAAKNTTYHYSTSMLLNAFSRRVFLWLGWLLISTSSLAATSTTYKVATEADDVVTRVLFDSIAHHFNLEIEYV
NYPSFNDILVAIETGNADFAANITYTDLRAQRFDFSRPTNIEYTYLYSYGGLRLPELRLVGIPKGTTYGTLLKEHYPYIQ
QVEYEGHLEALTLLESGRVDGVVDAINQLKPMLLKGLDVQLLNDQLPIQPVSIVTPKGKHSALLGKIEKYAHSAHVQRLL
RESIQKYQLDIRKQALRQSVVESGLNVQRVLRVKLENNPQYALYQPDGSVRGISADVVFQACEMLLLKCELVSNGQETWE
SMFDDLQDKSIDILAPITVSQQRKNLAYFSESYYHPQAILVKREHYKDDVYSNVSELVAERIGVIKDDFFEELLQQMLPN
KILFSYASQEEKVQALLNKEVDYIVLNRANFNLLLRESTEMLPIVEDTMIGSFYQYDIAIGFAKNPLGATLAPLFSRAIK
MLNTEQIIHTYDYQPNWRATLLAEKKYQRSTQWLFAMAFIVLFMVAFYLHGISHTDNLTKLRNRRALYNRYRRGLSPRLS
LVYLDVNTFKSINDQYGHEVGDKVLKQLAQRIEAVWRGRSYRIGGDEFILIGECSAKRLEHVVAQCERFMFVDAERDVSF
EVSVAIGIAKNRERTESLNEVMHQADIAMYRAKAESTQSPFQAASKVKGLHIV

Sequences:

>Translated_693_residues
MGVFTIKCAAKNTTYHYSTSMLLNAFSRRVFLWLGWLLISTSSLAATSTTYKVATEADDVVTRVLFDSIAHHFNLEIEYV
NYPSFNDILVAIETGNADFAANITYTDLRAQRFDFSRPTNIEYTYLYSYGGLRLPELRLVGIPKGTTYGTLLKEHYPYIQ
QVEYEGHLEALTLLESGRVDGVVDAINQLKPMLLKGLDVQLLNDQLPIQPVSIVTPKGKHSALLGKIEKYAHSAHVQRLL
RESIQKYQLDIRKQALRQSVVESGLNVQRVLRVKLENNPQYALYQPDGSVRGISADVVFQACEMLLLKCELVSNGQETWE
SMFDDLQDKSIDILAPITVSQQRKNLAYFSESYYHPQAILVKREHYKDDVYSNVSELVAERIGVIKDDFFEELLQQMLPN
KILFSYASQEEKVQALLNKEVDYIVLNRANFNLLLRESTEMLPIVEDTMIGSFYQYDIAIGFAKNPLGATLAPLFSRAIK
MLNTEQIIHTYDYQPNWRATLLAEKKYQRSTQWLFAMAFIVLFMVAFYLHGISHTDNLTKLRNRRALYNRYRRGLSPRLS
LVYLDVNTFKSINDQYGHEVGDKVLKQLAQRIEAVWRGRSYRIGGDEFILIGECSAKRLEHVVAQCERFMFVDAERDVSF
EVSVAIGIAKNRERTESLNEVMHQADIAMYRAKAESTQSPFQAASKVKGLHIV
>Mature_692_residues
GVFTIKCAAKNTTYHYSTSMLLNAFSRRVFLWLGWLLISTSSLAATSTTYKVATEADDVVTRVLFDSIAHHFNLEIEYVN
YPSFNDILVAIETGNADFAANITYTDLRAQRFDFSRPTNIEYTYLYSYGGLRLPELRLVGIPKGTTYGTLLKEHYPYIQQ
VEYEGHLEALTLLESGRVDGVVDAINQLKPMLLKGLDVQLLNDQLPIQPVSIVTPKGKHSALLGKIEKYAHSAHVQRLLR
ESIQKYQLDIRKQALRQSVVESGLNVQRVLRVKLENNPQYALYQPDGSVRGISADVVFQACEMLLLKCELVSNGQETWES
MFDDLQDKSIDILAPITVSQQRKNLAYFSESYYHPQAILVKREHYKDDVYSNVSELVAERIGVIKDDFFEELLQQMLPNK
ILFSYASQEEKVQALLNKEVDYIVLNRANFNLLLRESTEMLPIVEDTMIGSFYQYDIAIGFAKNPLGATLAPLFSRAIKM
LNTEQIIHTYDYQPNWRATLLAEKKYQRSTQWLFAMAFIVLFMVAFYLHGISHTDNLTKLRNRRALYNRYRRGLSPRLSL
VYLDVNTFKSINDQYGHEVGDKVLKQLAQRIEAVWRGRSYRIGGDEFILIGECSAKRLEHVVAQCERFMFVDAERDVSFE
VSVAIGIAKNRERTESLNEVMHQADIAMYRAKAESTQSPFQAASKVKGLHIV

Specific function: Probable signaling protein whose physiological role is not yet known [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI87081977, Length=163, Percent_Identity=30.0613496932515, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI87081881, Length=154, Percent_Identity=34.4155844155844, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787802, Length=157, Percent_Identity=31.2101910828025, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788381, Length=157, Percent_Identity=31.8471337579618, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1787262, Length=182, Percent_Identity=26.9230769230769, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1786584, Length=188, Percent_Identity=25, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788956, Length=156, Percent_Identity=32.0512820512821, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1787816, Length=150, Percent_Identity=35.3333333333333, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1787541, Length=150, Percent_Identity=30, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI145693134, Length=152, Percent_Identity=32.2368421052632, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 79310; Mature: 79179

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVFTIKCAAKNTTYHYSTSMLLNAFSRRVFLWLGWLLISTSSLAATSTTYKVATEADDV
CCEEEEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEEECCHHHH
VTRVLFDSIAHHFNLEIEYVNYPSFNDILVAIETGNADFAANITYTDLRAQRFDFSRPTN
HHHHHHHHHHHHEEEEEEEECCCCCCCEEEEEEECCCCEEEEEEEEHHHHHHCCCCCCCC
IEYTYLYSYGGLRLPELRLVGIPKGTTYGTLLKEHYPYIQQVEYEGHLEALTLLESGRVD
CEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHCCHHHEECCCCCHHHHHHHHCCCCC
GVVDAINQLKPMLLKGLDVQLLNDQLPIQPVSIVTPKGKHSALLGKIEKYAHSAHVQRLL
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
RESIQKYQLDIRKQALRQSVVESGLNVQRVLRVKLENNPQYALYQPDGSVRGISADVVFQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEECCCCCCCCCHHHHHHH
ACEMLLLKCELVSNGQETWESMFDDLQDKSIDILAPITVSQQRKNLAYFSESYYHPQAIL
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEE
VKREHYKDDVYSNVSELVAERIGVIKDDFFEELLQQMLPNKILFSYASQEEKVQALLNKE
EEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHCCC
VDYIVLNRANFNLLLRESTEMLPIVEDTMIGSFYQYDIAIGFAKNPLGATLAPLFSRAIK
CCEEEEECCCCEEEEECCCCCCCCHHHHHHCCEEEEEEEEEECCCCCCHHHHHHHHHHHH
MLNTEQIIHTYDYQPNWRATLLAEKKYQRSTQWLFAMAFIVLFMVAFYLHGISHTDNLTK
HHCHHHHHEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LRNRRALYNRYRRGLSPRLSLVYLDVNTFKSINDQYGHEVGDKVLKQLAQRIEAVWRGRS
HHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YRIGGDEFILIGECSAKRLEHVVAQCERFMFVDAERDVSFEVSVAIGIAKNRERTESLNE
EEECCCCEEEEECCCHHHHHHHHHHHHHEEEEECCCCCEEEEEEEEEECCCHHHHHHHHH
VMHQADIAMYRAKAESTQSPFQAASKVKGLHIV
HHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
GVFTIKCAAKNTTYHYSTSMLLNAFSRRVFLWLGWLLISTSSLAATSTTYKVATEADDV
CEEEEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEEECCHHHH
VTRVLFDSIAHHFNLEIEYVNYPSFNDILVAIETGNADFAANITYTDLRAQRFDFSRPTN
HHHHHHHHHHHHEEEEEEEECCCCCCCEEEEEEECCCCEEEEEEEEHHHHHHCCCCCCCC
IEYTYLYSYGGLRLPELRLVGIPKGTTYGTLLKEHYPYIQQVEYEGHLEALTLLESGRVD
CEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHCCHHHEECCCCCHHHHHHHHCCCCC
GVVDAINQLKPMLLKGLDVQLLNDQLPIQPVSIVTPKGKHSALLGKIEKYAHSAHVQRLL
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
RESIQKYQLDIRKQALRQSVVESGLNVQRVLRVKLENNPQYALYQPDGSVRGISADVVFQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEECCCCCCCCCHHHHHHH
ACEMLLLKCELVSNGQETWESMFDDLQDKSIDILAPITVSQQRKNLAYFSESYYHPQAIL
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEE
VKREHYKDDVYSNVSELVAERIGVIKDDFFEELLQQMLPNKILFSYASQEEKVQALLNKE
EEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHCCC
VDYIVLNRANFNLLLRESTEMLPIVEDTMIGSFYQYDIAIGFAKNPLGATLAPLFSRAIK
CCEEEEECCCCEEEEECCCCCCCCHHHHHHCCEEEEEEEEEECCCCCCHHHHHHHHHHHH
MLNTEQIIHTYDYQPNWRATLLAEKKYQRSTQWLFAMAFIVLFMVAFYLHGISHTDNLTK
HHCHHHHHEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LRNRRALYNRYRRGLSPRLSLVYLDVNTFKSINDQYGHEVGDKVLKQLAQRIEAVWRGRS
HHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YRIGGDEFILIGECSAKRLEHVVAQCERFMFVDAERDVSFEVSVAIGIAKNRERTESLNE
EEECCCCEEEEECCCHHHHHHHHHHHHHEEEEECCCCCEEEEEEEEEECCCHHHHHHHHH
VMHQADIAMYRAKAESTQSPFQAASKVKGLHIV
HHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11728710 [H]