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The map label for this gene is aaeR [H]

Identifier: 227081222

GI number: 227081222

Start: 1076517

End: 1077416

Strand: Reverse

Name: aaeR [H]

Synonym: VCM66_1004

Alternate gene names: 227081222

Gene position: 1077416-1076517 (Counterclockwise)

Preceding gene: 227081230

Following gene: 227081218

Centisome position: 37.25

GC content: 47.0

Gene sequence:

>900_bases
TTGCAACATAATGTGTCAGAAACGATGAAACTAGATGACCTAAACCTCTTTCGACTGGTTGTCGAAAATGGAAGCTATAC
CTCAACTTCAAAAAAAACCATGATCCCTGTGGCAACGATCACAAGACGGATCCAAGCCTTGGAGGACTCCCTAAACCTTA
GGTTACTCAATCGTCACGCTCGTAAACTGACTCTGACCGAAGCGGGCGAACGTTTCTACAAAGATTGCTCTCCCCTGCTG
GAGCGTCTCGCTTCAATGACAGAAGAGATCACCGATGAATGTCGTGGTGCGTCGGGGCGCATTCGTATTTCTGCACCATC
CAATCTGACAAAACGAATGATGATGCCGATGTTCAATGCCTTTATGGAAAAATATCCCGATATTCATATTGAGCTGATGA
TGAGTAACCAAGCCGACGATCTCGACCCCACTGAATGGGATGTGATTTTCCGAGTTGGCCCACAGCGTGATTCCAGCTTG
ATAGCTCGTAAAATCGGTGAAGTGAAAGACATTTTGGTTGCCAGCCCTCAATACTTGTCGAGTCATCCGCAACCGACTCA
TGCTGAAGAGTTGCATCAACATCAGTTGCTCAAAGGTTATCCATTGCTCAAATGGCAACTGACCAACAGCCAAGGTGAAA
CTGTCGTCAACAGTGATCGCGGCCGTTTTCAAGCCAGCGCACTGAATGTTGTTCGCAGCGCATGTTCAGAAGGTTTAGGC
ATTACGCTCATGCCCGATGTGATGCTGCGTGAATTTCTTGAGGATGGCAGTTTGGTACAAGTACTGTCGGATTGGAGCTC
AAACCCGCGGGATATCTACATGCTGTATAACCACAAAGATCACCAGCCGGAAAAAGTGCGCCTGTTTATTGATTTTGTGA
TTGGCTACCACCTGCAATAA

Upstream 100 bases:

>100_bases
AAATTTAATCAATCACAAGATGTATGGTTTTCAATAAATCATCTTACAAGTTGACATCACGCAAAATTTATCGGCTTATA
TGTGTATAAATTAGCGATAG

Downstream 100 bases:

>100_bases
ACACTCGTCTTTTTATAAAGCATTTCAATAGAAAAAGGGCTTCGCGCCCTTTTTCTTACTGTTGTACGTACCCAATATTT
AGAGATACTCCACATACTTG

Product: transcriptional regulator, LysR family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MQHNVSETMKLDDLNLFRLVVENGSYTSTSKKTMIPVATITRRIQALEDSLNLRLLNRHARKLTLTEAGERFYKDCSPLL
ERLASMTEEITDECRGASGRIRISAPSNLTKRMMMPMFNAFMEKYPDIHIELMMSNQADDLDPTEWDVIFRVGPQRDSSL
IARKIGEVKDILVASPQYLSSHPQPTHAEELHQHQLLKGYPLLKWQLTNSQGETVVNSDRGRFQASALNVVRSACSEGLG
ITLMPDVMLREFLEDGSLVQVLSDWSSNPRDIYMLYNHKDHQPEKVRLFIDFVIGYHLQ

Sequences:

>Translated_299_residues
MQHNVSETMKLDDLNLFRLVVENGSYTSTSKKTMIPVATITRRIQALEDSLNLRLLNRHARKLTLTEAGERFYKDCSPLL
ERLASMTEEITDECRGASGRIRISAPSNLTKRMMMPMFNAFMEKYPDIHIELMMSNQADDLDPTEWDVIFRVGPQRDSSL
IARKIGEVKDILVASPQYLSSHPQPTHAEELHQHQLLKGYPLLKWQLTNSQGETVVNSDRGRFQASALNVVRSACSEGLG
ITLMPDVMLREFLEDGSLVQVLSDWSSNPRDIYMLYNHKDHQPEKVRLFIDFVIGYHLQ
>Mature_299_residues
MQHNVSETMKLDDLNLFRLVVENGSYTSTSKKTMIPVATITRRIQALEDSLNLRLLNRHARKLTLTEAGERFYKDCSPLL
ERLASMTEEITDECRGASGRIRISAPSNLTKRMMMPMFNAFMEKYPDIHIELMMSNQADDLDPTEWDVIFRVGPQRDSSL
IARKIGEVKDILVASPQYLSSHPQPTHAEELHQHQLLKGYPLLKWQLTNSQGETVVNSDRGRFQASALNVVRSACSEGLG
ITLMPDVMLREFLEDGSLVQVLSDWSSNPRDIYMLYNHKDHQPEKVRLFIDFVIGYHLQ

Specific function: Activates transcription of the aaeXAB operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789639, Length=292, Percent_Identity=28.0821917808219, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1786401, Length=287, Percent_Identity=28.2229965156794, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI87081978, Length=290, Percent_Identity=28.2758620689655, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI1789440, Length=291, Percent_Identity=28.1786941580756, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI145693193, Length=291, Percent_Identity=26.1168384879725, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787589, Length=288, Percent_Identity=26.0416666666667, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=26.4604810996564, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789173, Length=287, Percent_Identity=24.7386759581882, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790011, Length=300, Percent_Identity=22.6666666666667, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788481, Length=303, Percent_Identity=22.4422442244224, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI157672245, Length=175, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787601, Length=125, Percent_Identity=27.2, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082132, Length=174, Percent_Identity=28.1609195402299, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34249; Mature: 34249

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHNVSETMKLDDLNLFRLVVENGSYTSTSKKTMIPVATITRRIQALEDSLNLRLLNRHA
CCCCHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
RKLTLTEAGERFYKDCSPLLERLASMTEEITDECRGASGRIRISAPSNLTKRMMMPMFNA
HEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHH
FMEKYPDIHIELMMSNQADDLDPTEWDVIFRVGPQRDSSLIARKIGEVKDILVASPQYLS
HHHHCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHH
SHPQPTHAEELHQHQLLKGYPLLKWQLTNSQGETVVNSDRGRFQASALNVVRSACSEGLG
CCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHCCCC
ITLMPDVMLREFLEDGSLVQVLSDWSSNPRDIYMLYNHKDHQPEKVRLFIDFVIGYHLQ
EEECHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHEEEHHHHCCC
>Mature Secondary Structure
MQHNVSETMKLDDLNLFRLVVENGSYTSTSKKTMIPVATITRRIQALEDSLNLRLLNRHA
CCCCHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
RKLTLTEAGERFYKDCSPLLERLASMTEEITDECRGASGRIRISAPSNLTKRMMMPMFNA
HEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHH
FMEKYPDIHIELMMSNQADDLDPTEWDVIFRVGPQRDSSLIARKIGEVKDILVASPQYLS
HHHHCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHH
SHPQPTHAEELHQHQLLKGYPLLKWQLTNSQGETVVNSDRGRFQASALNVVRSACSEGLG
CCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHCCCC
ITLMPDVMLREFLEDGSLVQVLSDWSSNPRDIYMLYNHKDHQPEKVRLFIDFVIGYHLQ
EEECHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]