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The map label for this gene is rnfE [H]

Identifier: 227081186

GI number: 227081186

Start: 1038461

End: 1039153

Strand: Reverse

Name: rnfE [H]

Synonym: VCM66_0968

Alternate gene names: 227081186

Gene position: 1039153-1038461 (Counterclockwise)

Preceding gene: 227081187

Following gene: 227081185

Centisome position: 35.93

GC content: 51.37

Gene sequence:

>693_bases
ATGAATGAAAACCGAACTCTGATGTTAAATGGCATGTGGAACAACAACCCAGCCTTAGTGCAATTACTGGGATTGTGTCC
GCTGCTCGCCGTTTCCTCCACCGTGACCAATGCCCTCGGATTAGGCATTGCTACGCTTCTTGTTCTCGTCGGCTCGAATG
TCACCGTCTCTCTCGTGCGTGATTATGTGCCGAAAGAAGTCCGAATTCCGGTGTTCGTGATGATCATCGCTTCGCTGGTG
ACTTGCGTACAGTTACTGATGAACGCCTACGCTTACGGGCTTTATCTCTCGCTCGGCATTTTTATTCCGCTGATTGTGAC
AAACTGCATCATCATAGGCCGTGCTGAAGCGTTTGCTTCAAAAAATGATGTGCTCCCAGCCGCGTTAGACGGATTTTGGA
TGGGATTGGGGATGACCTCAGTACTGGTTGTGCTCGGATCACTGCGCGAAATCATCGGCAATGGCACGCTGTTCGATGGC
GCGGACCTGCTACTCGGAGAATGGGCTAAAGTCCTACGCATCGAAGTGTTTCACTTTGACAGCGCCTTTTTGCTGGCACT
GCTGCCCCCCGGTGCCTTCATCGGCGTTGGCTTTTTGATCGCCGCTAAAAGCGTGATTGATAAACAGACTGCCGCTCGCC
AACCAAAACAACAAAAGCAAGCCATAGAGCGTGCACGCGTGACCAATGTCTAA

Upstream 100 bases:

>100_bases
CCTCGCGCTGTCGTACTGGCGGTCAAAAAAGCCGTGGAGTATGTCAACCAACATCAACAACAGCTGCACAATCAGCCGAA
TCCGTGTGAGGGTCAATAAC

Downstream 100 bases:

>100_bases
CCTGATCAAGGAAGCCAACCCGTCTAATGAATAACGCGAAACGGATTGAAATTTTAGAACGGCTGAGGGCTCACAACCCA
AAACCCGAAACCGAATTAAA

Product: electron transport complex RsxE subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MNENRTLMLNGMWNNNPALVQLLGLCPLLAVSSTVTNALGLGIATLLVLVGSNVTVSLVRDYVPKEVRIPVFVMIIASLV
TCVQLLMNAYAYGLYLSLGIFIPLIVTNCIIIGRAEAFASKNDVLPAALDGFWMGLGMTSVLVVLGSLREIIGNGTLFDG
ADLLLGEWAKVLRIEVFHFDSAFLLALLPPGAFIGVGFLIAAKSVIDKQTAARQPKQQKQAIERARVTNV

Sequences:

>Translated_230_residues
MNENRTLMLNGMWNNNPALVQLLGLCPLLAVSSTVTNALGLGIATLLVLVGSNVTVSLVRDYVPKEVRIPVFVMIIASLV
TCVQLLMNAYAYGLYLSLGIFIPLIVTNCIIIGRAEAFASKNDVLPAALDGFWMGLGMTSVLVVLGSLREIIGNGTLFDG
ADLLLGEWAKVLRIEVFHFDSAFLLALLPPGAFIGVGFLIAAKSVIDKQTAARQPKQQKQAIERARVTNV
>Mature_230_residues
MNENRTLMLNGMWNNNPALVQLLGLCPLLAVSSTVTNALGLGIATLLVLVGSNVTVSLVRDYVPKEVRIPVFVMIIASLV
TCVQLLMNAYAYGLYLSLGIFIPLIVTNCIIIGRAEAFASKNDVLPAALDGFWMGLGMTSVLVVLGSLREIIGNGTLFDG
ADLLLGEWAKVLRIEVFHFDSAFLLALLPPGAFIGVGFLIAAKSVIDKQTAARQPKQQKQAIERARVTNV

Specific function: May be part of a membrane complex involved in electron transport [H]

COG id: COG4660

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfE

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family [H]

Homologues:

Organism=Escherichia coli, GI1787919, Length=209, Percent_Identity=66.9856459330144, Blast_Score=272, Evalue=1e-74,
Organism=Escherichia coli, GI1787914, Length=156, Percent_Identity=32.0512820512821, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003667
- InterPro:   IPR010968 [H]

Pfam domain/function: PF02508 Rnf-Nqr [H]

EC number: NA

Molecular weight: Translated: 24711; Mature: 24711

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNENRTLMLNGMWNNNPALVQLLGLCPLLAVSSTVTNALGLGIATLLVLVGSNVTVSLVR
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
DYVPKEVRIPVFVMIIASLVTCVQLLMNAYAYGLYLSLGIFIPLIVTNCIIIGRAEAFAS
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHCC
KNDVLPAALDGFWMGLGMTSVLVVLGSLREIIGNGTLFDGADLLLGEWAKVLRIEVFHFD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHEECC
SAFLLALLPPGAFIGVGFLIAAKSVIDKQTAARQPKQQKQAIERARVTNV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNENRTLMLNGMWNNNPALVQLLGLCPLLAVSSTVTNALGLGIATLLVLVGSNVTVSLVR
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
DYVPKEVRIPVFVMIIASLVTCVQLLMNAYAYGLYLSLGIFIPLIVTNCIIIGRAEAFAS
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHCC
KNDVLPAALDGFWMGLGMTSVLVVLGSLREIIGNGTLFDGADLLLGEWAKVLRIEVFHFD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHEECC
SAFLLALLPPGAFIGVGFLIAAKSVIDKQTAARQPKQQKQAIERARVTNV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA