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The map label for this gene is purF [H]

Identifier: 227081178

GI number: 227081178

Start: 1031788

End: 1033302

Strand: Direct

Name: purF [H]

Synonym: VCM66_0960

Alternate gene names: 227081178

Gene position: 1031788-1033302 (Clockwise)

Preceding gene: 227081177

Following gene: 227081182

Centisome position: 35.67

GC content: 48.65

Gene sequence:

>1515_bases
ATGTGTGGTATTGTTGGAATCGTGGGTACAACGCCTGTAAACCAGTCAATTTATGACGCTTTGACGGTCTTGCAGCATCG
TGGCCAAGATGCCGCGGGTATTTGTACCATAGAAAGCAATCGTTTTCGTCTGCGTAAGGCAAACGGATTAGTCAGGGATG
TCTTTGAAGCGAAACACATGCAACGCCTGCAAGGCAACGTCGGGATCGGCCATGTGCGTTACCCCACCGCAGGTAGCTCT
AGCGCCTCAGAAGCTCAACCTTTCTATGTCAACTCTCCTTTCGGTATCACACTTGCTCACAACGGTAATCTGACCAACGC
GAACCAAGTGCGTCAAAAGCTGTTTGAAAAAGACCGTCGTCATGTGAATACCACTTCGGATTCAGAGGTGTTGCTCAACG
TACTGGCTCATGAAATTGATACCGTAAAGGGCAATGTCACCGCCGACGATGTGTTCCGTGCAATCAGCAATGTTCACCGT
ACCATTCGAGGTGCTTATGCGGTGGCGGCGATGATCATTGGTCACGGTATGATTGCGTTTCGTGACCCACACGGTATTCG
ACCATTGTGTCTCGGTAAGCGTGAAGTCAATGGTCAGCTCGAATATATGGTCGCCTCCGAATCGGTCGCACTGGATGCGG
TTGGGTTCGACTTCGTGCGAGATGTCGCGCCCGGTGAAGCGATTTATGCCACTTTCGACGGTGAGCTCTACACCAAACAG
TGTGCCGATAATCCAGCACTCAACCCATGCATCTTTGAATTTGTCTACTTTGCGCGCCCTGATTCGTTTATCGACAAGAT
TTCTGTTTATAGCGCTCGTGTAGAGATGGGTAAGCGTTTAGGTGAGCGCATCAAAAATGATTACAGCGATCTCGATATCG
ATGTAGTCATCCCGATTCCTGAAACCTCTTGTGATATCGCCCTACAAATTGCACAGGCGATTGATAAACCATATCGCCAA
GGCTTTGTTAAAAACCGCTATGTTGGCCGAACCTTTATCATGCCGGGTCAGCAGCAACGTAAGAAATCGGTACGCCGCAA
ACTCAATGCGATTCGCTCAGAGTTTAAAGATAAAAACGTACTACTGGTGGATGATTCTATCGTGCGTGGCACTACCTCAG
AGCAGATCATTGAGATGGCTCGTGATTCTGGGGCGAAGAAAGTCTACATCGTCTCTGCTGCCCCTGAGATTCGCTTCCCG
AACGTGTATGGTATTGATATGCCAAGTGCCAATGAGCTGATTGCTCATGGTCGTGATAACGATGCTATCTGTAAGCAGAT
TGGTGCCGATGCCTTGATATTCCAAACTTTAGAGGATTTGGTGGAAGCGGTTCGCTGTGGCAACCCTGATATCGTCAAGT
TTGAAGCTTCGGTCTTTAATGGCGAATACGTGACGGGTGACATCGATCAGCAGTACTTAGATTTTCTCGAGTCGATGCGC
AGTGATGACGCGAAAGTGCAGCGTGAAATTCAACAAGATCTGGCTAACCTTGAGCTGCATAACGAAGGCGCTTAA

Upstream 100 bases:

>100_bases
GTCGCGTTATTGCTCCGTTCTTTGAACACCTAAAAGCAACATCAAGTTTTCTCTCTGGCGCGATTTAGCGCCGGAGACGG
TTGAAATCGAGGATTAGGAC

Downstream 100 bases:

>100_bases
GCAAACAAACGGGTTCGGATAATCATCAAAAAGGCTTCCTGCGGGAAGCCTTTTTCGTTATCCCCCTCACGACCGCTACG
CTTGGGTGAGGGGATGAGGT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MCGIVGIVGTTPVNQSIYDALTVLQHRGQDAAGICTIESNRFRLRKANGLVRDVFEAKHMQRLQGNVGIGHVRYPTAGSS
SASEAQPFYVNSPFGITLAHNGNLTNANQVRQKLFEKDRRHVNTTSDSEVLLNVLAHEIDTVKGNVTADDVFRAISNVHR
TIRGAYAVAAMIIGHGMIAFRDPHGIRPLCLGKREVNGQLEYMVASESVALDAVGFDFVRDVAPGEAIYATFDGELYTKQ
CADNPALNPCIFEFVYFARPDSFIDKISVYSARVEMGKRLGERIKNDYSDLDIDVVIPIPETSCDIALQIAQAIDKPYRQ
GFVKNRYVGRTFIMPGQQQRKKSVRRKLNAIRSEFKDKNVLLVDDSIVRGTTSEQIIEMARDSGAKKVYIVSAAPEIRFP
NVYGIDMPSANELIAHGRDNDAICKQIGADALIFQTLEDLVEAVRCGNPDIVKFEASVFNGEYVTGDIDQQYLDFLESMR
SDDAKVQREIQQDLANLELHNEGA

Sequences:

>Translated_504_residues
MCGIVGIVGTTPVNQSIYDALTVLQHRGQDAAGICTIESNRFRLRKANGLVRDVFEAKHMQRLQGNVGIGHVRYPTAGSS
SASEAQPFYVNSPFGITLAHNGNLTNANQVRQKLFEKDRRHVNTTSDSEVLLNVLAHEIDTVKGNVTADDVFRAISNVHR
TIRGAYAVAAMIIGHGMIAFRDPHGIRPLCLGKREVNGQLEYMVASESVALDAVGFDFVRDVAPGEAIYATFDGELYTKQ
CADNPALNPCIFEFVYFARPDSFIDKISVYSARVEMGKRLGERIKNDYSDLDIDVVIPIPETSCDIALQIAQAIDKPYRQ
GFVKNRYVGRTFIMPGQQQRKKSVRRKLNAIRSEFKDKNVLLVDDSIVRGTTSEQIIEMARDSGAKKVYIVSAAPEIRFP
NVYGIDMPSANELIAHGRDNDAICKQIGADALIFQTLEDLVEAVRCGNPDIVKFEASVFNGEYVTGDIDQQYLDFLESMR
SDDAKVQREIQQDLANLELHNEGA
>Mature_504_residues
MCGIVGIVGTTPVNQSIYDALTVLQHRGQDAAGICTIESNRFRLRKANGLVRDVFEAKHMQRLQGNVGIGHVRYPTAGSS
SASEAQPFYVNSPFGITLAHNGNLTNANQVRQKLFEKDRRHVNTTSDSEVLLNVLAHEIDTVKGNVTADDVFRAISNVHR
TIRGAYAVAAMIIGHGMIAFRDPHGIRPLCLGKREVNGQLEYMVASESVALDAVGFDFVRDVAPGEAIYATFDGELYTKQ
CADNPALNPCIFEFVYFARPDSFIDKISVYSARVEMGKRLGERIKNDYSDLDIDVVIPIPETSCDIALQIAQAIDKPYRQ
GFVKNRYVGRTFIMPGQQQRKKSVRRKLNAIRSEFKDKNVLLVDDSIVRGTTSEQIIEMARDSGAKKVYIVSAAPEIRFP
NVYGIDMPSANELIAHGRDNDAICKQIGADALIFQTLEDLVEAVRCGNPDIVKFEASVFNGEYVTGDIDQQYLDFLESMR
SDDAKVQREIQQDLANLELHNEGA

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=475, Percent_Identity=36.6315789473684, Blast_Score=271, Evalue=1e-72,
Organism=Escherichia coli, GI1788651, Length=507, Percent_Identity=76.1341222879684, Blast_Score=795, Evalue=0.0,
Organism=Escherichia coli, GI1790167, Length=223, Percent_Identity=30.9417040358744, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=463, Percent_Identity=34.341252699784, Blast_Score=242, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6323958, Length=510, Percent_Identity=50.1960784313725, Blast_Score=481, Evalue=1e-137,
Organism=Drosophila melanogaster, GI24659598, Length=494, Percent_Identity=35.2226720647773, Blast_Score=273, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24659604, Length=470, Percent_Identity=35.7446808510638, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI28573187, Length=499, Percent_Identity=33.4669338677355, Blast_Score=253, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55898; Mature: 55898

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIVGTTPVNQSIYDALTVLQHRGQDAAGICTIESNRFRLRKANGLVRDVFEAKHM
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEHHCCHHHHHHHHHHH
QRLQGNVGIGHVRYPTAGSSSASEAQPFYVNSPFGITLAHNGNLTNANQVRQKLFEKDRR
HHHCCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHH
HVNTTSDSEVLLNVLAHEIDTVKGNVTADDVFRAISNVHRTIRGAYAVAAMIIGHGMIAF
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
RDPHGIRPLCLGKREVNGQLEYMVASESVALDAVGFDFVRDVAPGEAIYATFDGELYTKQ
ECCCCCCCCCCCCCCCCCEEEEEEECCCEEEEHHHHHHHHHCCCCCEEEEEECCCCHHHH
CADNPALNPCIFEFVYFARPDSFIDKISVYSARVEMGKRLGERIKNDYSDLDIDVVIPIP
HCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
ETSCDIALQIAQAIDKPYRQGFVKNRYVGRTFIMPGQQQRKKSVRRKLNAIRSEFKDKNV
CCCHHHHHHHHHHHCHHHHCCHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCE
LLVDDSIVRGTTSEQIIEMARDSGAKKVYIVSAAPEIRFPNVYGIDMPSANELIAHGRDN
EEECCCHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEECCCCCHHHHHHCCCCC
DAICKQIGADALIFQTLEDLVEAVRCGNPDIVKFEASVFNGEYVTGDIDQQYLDFLESMR
HHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCEEECCCCHHHHHHHHHHC
SDDAKVQREIQQDLANLELHNEGA
CCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MCGIVGIVGTTPVNQSIYDALTVLQHRGQDAAGICTIESNRFRLRKANGLVRDVFEAKHM
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEHHCCHHHHHHHHHHH
QRLQGNVGIGHVRYPTAGSSSASEAQPFYVNSPFGITLAHNGNLTNANQVRQKLFEKDRR
HHHCCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHH
HVNTTSDSEVLLNVLAHEIDTVKGNVTADDVFRAISNVHRTIRGAYAVAAMIIGHGMIAF
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
RDPHGIRPLCLGKREVNGQLEYMVASESVALDAVGFDFVRDVAPGEAIYATFDGELYTKQ
ECCCCCCCCCCCCCCCCCEEEEEEECCCEEEEHHHHHHHHHCCCCCEEEEEECCCCHHHH
CADNPALNPCIFEFVYFARPDSFIDKISVYSARVEMGKRLGERIKNDYSDLDIDVVIPIP
HCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
ETSCDIALQIAQAIDKPYRQGFVKNRYVGRTFIMPGQQQRKKSVRRKLNAIRSEFKDKNV
CCCHHHHHHHHHHHCHHHHCCHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCE
LLVDDSIVRGTTSEQIIEMARDSGAKKVYIVSAAPEIRFPNVYGIDMPSANELIAHGRDN
EEECCCHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEECCCCCHHHHHHCCCCC
DAICKQIGADALIFQTLEDLVEAVRCGNPDIVKFEASVFNGEYVTGDIDQQYLDFLESMR
HHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCEEECCCCHHHHHHHHHHC
SDDAKVQREIQQDLANLELHNEGA
CCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3047685; 6443594; 6277938; 9205837; 9278503; 3040734; 7037784; 9333323; 9514258 [H]