Click here to switch to the map view.

The map label for this gene is glnS [H]

Identifier: 227081171

GI number: 227081171

Start: 1020578

End: 1022248

Strand: Direct

Name: glnS [H]

Synonym: VCM66_0953

Alternate gene names: 227081171

Gene position: 1020578-1022248 (Clockwise)

Preceding gene: 227081170

Following gene: 227081172

Centisome position: 35.28

GC content: 46.56

Gene sequence:

>1671_bases
ATGAGTGAAGCTGAGGCTCGTCCATCGAACTTCATCCGCCAAATTATCGATAAAGATCTTGCGGATGGTAAACACACGAC
CGTGCATACTCGTTTCCCGCCGGAGCCGAATGGCTACCTGCACATCGGTCACGCCAAGTCAATCTGTCTGAATTTCGGTA
TTGCTCAGGATTATCAAGGGCAGTGCAACCTGCGCTTTGATGATACAAACCCAGAAAAAGAGAACCTTGAATATGTTGAG
TCGATCAAAAAAGATGTGACTTGGCTAGGTTTTGATTGGTCAGGTGAGGTGTGTTACTCCTCTGACTATTTTGACAAGTT
GTATGAATACGCGATTGAGCTGATTCAAAAAGGCTTGGCGTATGTGGATGAACTGACGCCTGAGCAAATTCGTGAATACC
GTGGCACTCTAACTGAGCCTGGTAAGCACAGCCCATACCGCGATCGTAGCGTAGAAGAAAACCTAGCGCTGTTTGAAAAA
ATGCGTGCGGGTGAATTTGCAGAAGGTCAAGCTTGTCTACGTGCAAAAATCGATATGGCATCGTCTTTTATCGTAATGCG
TGATCCTGTTTTGTACCGTGTGCGTTTTGCTGAACACCACCAAACGGGTGACAAGTGGTGCATCTATCCGATGTACGATT
TCACACACTGTATTTCTGATGCGCTCGAAGGCATTACGCACTCTATTTGTACTTTGGAATTCCAAGACAACCGCCGTCTG
TATGATTGGGTTCTTGATAACATCACGATTCCATGCCATCCACGCCAGTATGAATTCAGCCGCTTAAATCTCGAATATAC
CGTGATGTCTAAGCGTAAACTGAACCAGCTAGTGACCGAAAAATTGGTCACAGGTTGGGATGATCCACGTATGCCGACCA
TTTCAGGCCTGCGTCGCCGTGGCTTCACGCCAAGCGCTATTCGTGAATTCTGTAAGCGCATTGGTGTGACTAAGCAAGAA
AACATGATTGAATACAGTGCATTAGAGTCTTGTATTCGTGATGATCTTAACGAAAATGCGCCACGTGCGATGGCCGTTCT
GGATCCGGTTAAACTGGTGATTGAGAACTTCGCCGCAGGCACAGTGGAAACTTTGACGCTGGCTAACCATCCGAACAAAC
CTGAGATGGGCGATCGCGAAGTGCCATTTACCCGTGAGTTGTGGATTGAACGCGAAGATTTCCGCGAAGAAGCCAACAAG
AAATACAAGCGTTTGGTGTTAGGCAAAGAAGTGCGTCTGCGTGGTGCTTACGTCATCAAAGCTGAGCGCATCGAAAAAGA
TGAACAAGGTAATATCACCACAATCTTCTGTTCTTACGATCCTGAAACCTTGGGTAAAAACCCAGCCGATGGTCGTAAAG
TGAAAGGTGTTATTCACTGGGTATCTGCAGAAAAAGGCGTTCCTGCTGAGTTTCGTCTCTACGAGCGTCTGTTCACTGTA
CCCAATCCAGGTGCGGCGGATAACTTTGCCGAAACCATTAACCCAGAATCTCTGGTAAAAGTTCAAGGTTATGTTGAGCC
AAGCTTAGTGGAAGCAAAACCTGAATTTGGTTATCAGTTTGAGCGTATGGGCTATTTCTGTGCCGATAACAAAGACTCTA
GCCCGCAGGCTTTGGTCTTTAACCGCACTGTTGGTTTGCGCGATAGTTTCGCCAAAATTGACGAAGAGTAA

Upstream 100 bases:

>100_bases
TGTGGATGATTAGCGGCAATATTTTCTGTTTTCTAGCCTTGATGGTACAAGAAGCAGAAACGCTTTCTGACAATACTAAA
TGAATTTTGAGGTGCTATAG

Downstream 100 bases:

>100_bases
GCCGAAAAACGAGAGTAAAATGCAAAGCCAGCCAACGTGCTGGCTTTGTTTTTAGGCGTGGAGAAAGCGGTGTTGCCTCA
CGCTAGGACTGAAATTTGTG

Product: glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamine--tRNA ligase; GlnRS [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MSEAEARPSNFIRQIIDKDLADGKHTTVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYQGQCNLRFDDTNPEKENLEYVE
SIKKDVTWLGFDWSGEVCYSSDYFDKLYEYAIELIQKGLAYVDELTPEQIREYRGTLTEPGKHSPYRDRSVEENLALFEK
MRAGEFAEGQACLRAKIDMASSFIVMRDPVLYRVRFAEHHQTGDKWCIYPMYDFTHCISDALEGITHSICTLEFQDNRRL
YDWVLDNITIPCHPRQYEFSRLNLEYTVMSKRKLNQLVTEKLVTGWDDPRMPTISGLRRRGFTPSAIREFCKRIGVTKQE
NMIEYSALESCIRDDLNENAPRAMAVLDPVKLVIENFAAGTVETLTLANHPNKPEMGDREVPFTRELWIEREDFREEANK
KYKRLVLGKEVRLRGAYVIKAERIEKDEQGNITTIFCSYDPETLGKNPADGRKVKGVIHWVSAEKGVPAEFRLYERLFTV
PNPGAADNFAETINPESLVKVQGYVEPSLVEAKPEFGYQFERMGYFCADNKDSSPQALVFNRTVGLRDSFAKIDEE

Sequences:

>Translated_556_residues
MSEAEARPSNFIRQIIDKDLADGKHTTVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYQGQCNLRFDDTNPEKENLEYVE
SIKKDVTWLGFDWSGEVCYSSDYFDKLYEYAIELIQKGLAYVDELTPEQIREYRGTLTEPGKHSPYRDRSVEENLALFEK
MRAGEFAEGQACLRAKIDMASSFIVMRDPVLYRVRFAEHHQTGDKWCIYPMYDFTHCISDALEGITHSICTLEFQDNRRL
YDWVLDNITIPCHPRQYEFSRLNLEYTVMSKRKLNQLVTEKLVTGWDDPRMPTISGLRRRGFTPSAIREFCKRIGVTKQE
NMIEYSALESCIRDDLNENAPRAMAVLDPVKLVIENFAAGTVETLTLANHPNKPEMGDREVPFTRELWIEREDFREEANK
KYKRLVLGKEVRLRGAYVIKAERIEKDEQGNITTIFCSYDPETLGKNPADGRKVKGVIHWVSAEKGVPAEFRLYERLFTV
PNPGAADNFAETINPESLVKVQGYVEPSLVEAKPEFGYQFERMGYFCADNKDSSPQALVFNRTVGLRDSFAKIDEE
>Mature_555_residues
SEAEARPSNFIRQIIDKDLADGKHTTVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYQGQCNLRFDDTNPEKENLEYVES
IKKDVTWLGFDWSGEVCYSSDYFDKLYEYAIELIQKGLAYVDELTPEQIREYRGTLTEPGKHSPYRDRSVEENLALFEKM
RAGEFAEGQACLRAKIDMASSFIVMRDPVLYRVRFAEHHQTGDKWCIYPMYDFTHCISDALEGITHSICTLEFQDNRRLY
DWVLDNITIPCHPRQYEFSRLNLEYTVMSKRKLNQLVTEKLVTGWDDPRMPTISGLRRRGFTPSAIREFCKRIGVTKQEN
MIEYSALESCIRDDLNENAPRAMAVLDPVKLVIENFAAGTVETLTLANHPNKPEMGDREVPFTRELWIEREDFREEANKK
YKRLVLGKEVRLRGAYVIKAERIEKDEQGNITTIFCSYDPETLGKNPADGRKVKGVIHWVSAEKGVPAEFRLYERLFTVP
NPGAADNFAETINPESLVKVQGYVEPSLVEAKPEFGYQFERMGYFCADNKDSSPQALVFNRTVGLRDSFAKIDEE

Specific function: Unknown

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI4826960, Length=532, Percent_Identity=47.3684210526316, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI62241042, Length=538, Percent_Identity=33.8289962825279, Blast_Score=282, Evalue=5e-76,
Organism=Escherichia coli, GI1786895, Length=555, Percent_Identity=80.9009009009009, Blast_Score=954, Evalue=0.0,
Organism=Escherichia coli, GI1788743, Length=318, Percent_Identity=22.6415094339623, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17543332, Length=535, Percent_Identity=43.5514018691589, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17511007, Length=511, Percent_Identity=34.4422700587084, Blast_Score=261, Evalue=5e-70,
Organism=Saccharomyces cerevisiae, GI6324742, Length=565, Percent_Identity=43.1858407079646, Blast_Score=414, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6321192, Length=526, Percent_Identity=35.171102661597, Blast_Score=306, Evalue=5e-84,
Organism=Drosophila melanogaster, GI17864488, Length=531, Percent_Identity=43.3145009416196, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24649466, Length=515, Percent_Identity=34.368932038835, Blast_Score=270, Evalue=2e-72,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR004514
- InterPro:   IPR022861
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C [H]

EC number: =6.1.1.18 [H]

Molecular weight: Translated: 64090; Mature: 63959

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAEARPSNFIRQIIDKDLADGKHTTVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYQG
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCHHEEHEECCCCCCCCC
QCNLRFDDTNPEKENLEYVESIKKDVTWLGFDWSGEVCYSSDYFDKLYEYAIELIQKGLA
CEEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHH
YVDELTPEQIREYRGTLTEPGKHSPYRDRSVEENLALFEKMRAGEFAEGQACLRAKIDMA
HHHHCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
SSFIVMRDPVLYRVRFAEHHQTGDKWCIYPMYDFTHCISDALEGITHSICTLEFQDNRRL
CCEEEEECCEEEEEEEHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHEEEEEECCCCCH
YDWVLDNITIPCHPRQYEFSRLNLEYTVMSKRKLNQLVTEKLVTGWDDPRMPTISGLRRR
HHHHHHCCEECCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHC
GFTPSAIREFCKRIGVTKQENMIEYSALESCIRDDLNENAPRAMAVLDPVKLVIENFAAG
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
TVETLTLANHPNKPEMGDREVPFTRELWIEREDFREEANKKYKRLVLGKEVRLRGAYVIK
CEEEEEECCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCEEECEEEEEE
AERIEKDEQGNITTIFCSYDPETLGKNPADGRKVKGVIHWVSAEKGVPAEFRLYERLFTV
HHHCCCCCCCCEEEEEECCCHHHCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHCC
PNPGAADNFAETINPESLVKVQGYVEPSLVEAKPEFGYQFERMGYFCADNKDSSPQALVF
CCCCCCCHHHHHCCCHHEEEEECCCCCHHCCCCCCCCCCHHHCCEEEECCCCCCCCEEEE
NRTVGLRDSFAKIDEE
ECCCCCCHHHHHCCCC
>Mature Secondary Structure 
SEAEARPSNFIRQIIDKDLADGKHTTVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYQG
CCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCHHEEHEECCCCCCCCC
QCNLRFDDTNPEKENLEYVESIKKDVTWLGFDWSGEVCYSSDYFDKLYEYAIELIQKGLA
CEEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHH
YVDELTPEQIREYRGTLTEPGKHSPYRDRSVEENLALFEKMRAGEFAEGQACLRAKIDMA
HHHHCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
SSFIVMRDPVLYRVRFAEHHQTGDKWCIYPMYDFTHCISDALEGITHSICTLEFQDNRRL
CCEEEEECCEEEEEEEHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHEEEEEECCCCCH
YDWVLDNITIPCHPRQYEFSRLNLEYTVMSKRKLNQLVTEKLVTGWDDPRMPTISGLRRR
HHHHHHCCEECCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHC
GFTPSAIREFCKRIGVTKQENMIEYSALESCIRDDLNENAPRAMAVLDPVKLVIENFAAG
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
TVETLTLANHPNKPEMGDREVPFTRELWIEREDFREEANKKYKRLVLGKEVRLRGAYVIK
CEEEEEECCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCEEECEEEEEE
AERIEKDEQGNITTIFCSYDPETLGKNPADGRKVKGVIHWVSAEKGVPAEFRLYERLFTV
HHHCCCCCCCCEEEEEECCCHHHCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHCC
PNPGAADNFAETINPESLVKVQGYVEPSLVEAKPEFGYQFERMGYFCADNKDSSPQALVF
CCCCCCCHHHHHCCCHHEEEEECCCCCHHCCCCCCCCCCHHHCCEEEECCCCCCCCEEEE
NRTVGLRDSFAKIDEE
ECCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA