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The map label for this gene is nagA

Identifier: 227081168

GI number: 227081168

Start: 1017154

End: 1018290

Strand: Reverse

Name: nagA

Synonym: VCM66_0950

Alternate gene names: 227081168

Gene position: 1018290-1017154 (Counterclockwise)

Preceding gene: 227081179

Following gene: 227081167

Centisome position: 35.2

GC content: 45.82

Gene sequence:

>1137_bases
ATGTATGCGCTAACGAACTGCAAAATCTACACAGGCAATGATGTGCTTGTGAAACATGCGGTAATCATCAACGGTGACAA
AATTGAAGCCGTTTGCCCTATCGAATCTCTTCCATCTGAGATGAATGTGGTCGACCTAAACGGTGCAAACCTCAGCCCTG
GTTTTATCGATTTACAGCTGAATGGCTGTGGTGGTGTGATGTTCAATGATGAAATCACAGCGGAAACCATCGATACCATG
CACAAAGCTAACCTTAAATCGGGCTGTACCAGCTTCCTTCCAACGCTGATCACTTCTTCCGATGAGAACATGCGCCAAGC
AATTGCCGCCGCTCGCGAATATCAGGCTAAATACCCAAATCAATCGCTTGGTCTTCATTTAGAAGGGCCTTATCTGAATG
TCATGAAAAAAGGGATCCACAGCGTTGACTTCATCCGCCCCTCTGACGATACGATGATTGATACGATTTGTGCCAATAGT
GATGTGATCGCCAAAGTCACTCTCGCGCCAGAAAATAATAAGCCTGAACATATTGAGAAGCTGGTTAAAGCGGGAATTGT
TGTTTCCATTGGACACACAAACGCGACCTATAGCGAAGCACGTAAAAGCTTTGAATCCGGCATTACCTTTGCGACCCACC
TCTTTAACGCAATGACCCCGATGGTAGGTCGTGAACCAGGAGTGGTCGGGGCGATTTATGATACACCTGAGGTGTATGCG
GGCATCATCGCCGATGGCTTCCATGTTGATTACGCAAACATCCGAATTGCCCACAAAATTAAGGGTGAAAAGCTCGTATT
AGTGACCGATGCCACAGCTCCTGCAGGCGCTGAAATGGATTACTTTATTTTTGTCGGTAAGAAAGTATATTACCGAGATG
GCAAATGTGTTGATGAAAATGGCACGCTTGGCGGTTCAGCCCTAACAATGATTGAAGCAGTTCAAAATACGGTTGAGCAT
GTCGGTATCGCTTTAGATGAAGCTCTGCGAATGGCTACTCTGTATCCAGCGAAAGCAATCGGTGTTGATGAGAAACTCGG
TCGTATCAAGAAAGGTATGATCGCCAACCTGACTGTTTTTGATCGTGACTTCAACGTAAAAGCGACGGTAGTTAACGGAC
AATACGAGCAGAATTGA

Upstream 100 bases:

>100_bases
ATTCGCGACCTGATTGAAGTGAAGCAGCTTGCGCTTTGGCTGTAACCTCGGTGAGTCGCTAAAATGAATCTAAAAATATC
GACAAGAGGATTAGCTGATT

Downstream 100 bases:

>100_bases
TAATGAATGGCGGACAGATTGGTAACGTAGATTTAGTTAAACAGCTAAACAGTGCGGCGGTTTACCGCTTGATTGACCAA
CAAGGCCCAATCAGTCGGAT

Product: N-acetylglucosamine-6-phosphate deacetylase

Products: NA

Alternate protein names: GlcNAc 6-P deacetylase

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MYALTNCKIYTGNDVLVKHAVIINGDKIEAVCPIESLPSEMNVVDLNGANLSPGFIDLQLNGCGGVMFNDEITAETIDTM
HKANLKSGCTSFLPTLITSSDENMRQAIAAAREYQAKYPNQSLGLHLEGPYLNVMKKGIHSVDFIRPSDDTMIDTICANS
DVIAKVTLAPENNKPEHIEKLVKAGIVVSIGHTNATYSEARKSFESGITFATHLFNAMTPMVGREPGVVGAIYDTPEVYA
GIIADGFHVDYANIRIAHKIKGEKLVLVTDATAPAGAEMDYFIFVGKKVYYRDGKCVDENGTLGGSALTMIEAVQNTVEH
VGIALDEALRMATLYPAKAIGVDEKLGRIKKGMIANLTVFDRDFNVKATVVNGQYEQN

Sequences:

>Translated_378_residues
MYALTNCKIYTGNDVLVKHAVIINGDKIEAVCPIESLPSEMNVVDLNGANLSPGFIDLQLNGCGGVMFNDEITAETIDTM
HKANLKSGCTSFLPTLITSSDENMRQAIAAAREYQAKYPNQSLGLHLEGPYLNVMKKGIHSVDFIRPSDDTMIDTICANS
DVIAKVTLAPENNKPEHIEKLVKAGIVVSIGHTNATYSEARKSFESGITFATHLFNAMTPMVGREPGVVGAIYDTPEVYA
GIIADGFHVDYANIRIAHKIKGEKLVLVTDATAPAGAEMDYFIFVGKKVYYRDGKCVDENGTLGGSALTMIEAVQNTVEH
VGIALDEALRMATLYPAKAIGVDEKLGRIKKGMIANLTVFDRDFNVKATVVNGQYEQN
>Mature_378_residues
MYALTNCKIYTGNDVLVKHAVIINGDKIEAVCPIESLPSEMNVVDLNGANLSPGFIDLQLNGCGGVMFNDEITAETIDTM
HKANLKSGCTSFLPTLITSSDENMRQAIAAAREYQAKYPNQSLGLHLEGPYLNVMKKGIHSVDFIRPSDDTMIDTICANS
DVIAKVTLAPENNKPEHIEKLVKAGIVVSIGHTNATYSEARKSFESGITFATHLFNAMTPMVGREPGVVGAIYDTPEVYA
GIIADGFHVDYANIRIAHKIKGEKLVLVTDATAPAGAEMDYFIFVGKKVYYRDGKCVDENGTLGGSALTMIEAVQNTVEH
VGIALDEALRMATLYPAKAIGVDEKLGRIKKGMIANLTVFDRDFNVKATVVNGQYEQN

Specific function: N-acetylglucosamine utilization. [C]

COG id: COG1820

COG function: function code G; N-acetylglucosamine-6-phosphate deacetylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nagA family

Homologues:

Organism=Homo sapiens, GI21361513, Length=420, Percent_Identity=31.9047619047619, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI224922791, Length=419, Percent_Identity=31.980906921241, Blast_Score=170, Evalue=2e-42,
Organism=Escherichia coli, GI1786892, Length=376, Percent_Identity=61.968085106383, Blast_Score=487, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI17553768, Length=388, Percent_Identity=32.7319587628866, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI19920392, Length=391, Percent_Identity=32.2250639386189, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI281361140, Length=405, Percent_Identity=31.1111111111111, Blast_Score=175, Evalue=6e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAGA_VIBCH (O32445)

Other databases:

- EMBL:   D87820
- EMBL:   AE003852
- PIR:   E82254
- PIR:   JC5649
- RefSeq:   NP_230640.1
- PDB:   3EGJ
- PDB:   3IV8
- PDBsum:   3EGJ
- PDBsum:   3IV8
- ProteinModelPortal:   O32445
- SMR:   O32445
- GeneID:   2614247
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0994
- TIGR:   VC_0994
- HOGENOM:   HBG464950
- OMA:   TSSDEDM
- ProtClustDB:   PRK11170
- BRENDA:   3.5.1.25
- InterPro:   IPR006680
- InterPro:   IPR003764
- InterPro:   IPR011059
- TIGRFAMs:   TIGR00221

Pfam domain/function: PF01979 Amidohydro_1; SSF51338 Metalo_hydrolase

EC number: =3.5.1.25

Molecular weight: Translated: 40956; Mature: 40956

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYALTNCKIYTGNDVLVKHAVIINGDKIEAVCPIESLPSEMNVVDLNGANLSPGFIDLQL
CEEEEEEEEEECCCEEEEEEEEEECCEEEEECCHHHCCCCCCEEEECCCCCCCCEEEEEE
NGCGGVMFNDEITAETIDTMHKANLKSGCTSFLPTLITSSDENMRQAIAAAREYQAKYPN
CCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
QSLGLHLEGPYLNVMKKGIHSVDFIRPSDDTMIDTICANSDVIAKVTLAPENNKPEHIEK
CCCCEEECCCHHHHHHHCCCCEEEECCCCCCEEEEEECCCCEEEEEEECCCCCCHHHHHH
LVKAGIVVSIGHTNATYSEARKSFESGITFATHLFNAMTPMVGREPGVVGAIYDTPEVYA
HHHCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHH
GIIADGFHVDYANIRIAHKIKGEKLVLVTDATAPAGAEMDYFIFVGKKVYYRDGKCVDEN
HHEECCEEEEEEEEEEEEEECCCEEEEEECCCCCCCCCEEEEEEECCEEEEECCEEECCC
GTLGGSALTMIEAVQNTVEHVGIALDEALRMATLYPAKAIGVDEKLGRIKKGMIANLTVF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHCCCEEEEEEE
DRDFNVKATVVNGQYEQN
ECCCCEEEEEECCCCCCC
>Mature Secondary Structure
MYALTNCKIYTGNDVLVKHAVIINGDKIEAVCPIESLPSEMNVVDLNGANLSPGFIDLQL
CEEEEEEEEEECCCEEEEEEEEEECCEEEEECCHHHCCCCCCEEEECCCCCCCCEEEEEE
NGCGGVMFNDEITAETIDTMHKANLKSGCTSFLPTLITSSDENMRQAIAAAREYQAKYPN
CCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
QSLGLHLEGPYLNVMKKGIHSVDFIRPSDDTMIDTICANSDVIAKVTLAPENNKPEHIEK
CCCCEEECCCHHHHHHHCCCCEEEECCCCCCEEEEEECCCCEEEEEEECCCCCCHHHHHH
LVKAGIVVSIGHTNATYSEARKSFESGITFATHLFNAMTPMVGREPGVVGAIYDTPEVYA
HHHCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHH
GIIADGFHVDYANIRIAHKIKGEKLVLVTDATAPAGAEMDYFIFVGKKVYYRDGKCVDEN
HHEECCEEEEEEEEEEEEEECCCEEEEEECCCCCCCCCEEEEEEECCEEEEECCEEECCC
GTLGGSALTMIEAVQNTVEHVGIALDEALRMATLYPAKAIGVDEKLGRIKKGMIANLTVF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHCCCEEEEEEE
DRDFNVKATVVNGQYEQN
ECCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9301118; 10952301