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The map label for this gene is asnB [H]

Identifier: 227081165

GI number: 227081165

Start: 1012396

End: 1014060

Strand: Reverse

Name: asnB [H]

Synonym: VCM66_0947

Alternate gene names: 227081165

Gene position: 1014060-1012396 (Counterclockwise)

Preceding gene: 227081166

Following gene: 227081162

Centisome position: 35.06

GC content: 46.49

Gene sequence:

>1665_bases
ATGTGCTCTATCTTTGGCATCTTAGACATTAAAACTGATGCAGCAACGCTAAGACCTATCGCCCTCGAAATGTCGAAAAA
ACTGCGCCATCGCGGTCCAGACTGGTCAGGTATCTATGCTTCTGATAAAGCGATTCTGGCCCATGAGCGTCTGGCCATCG
TGGGTTTAAATAGTGGCGCACAGCCTCTTTATAGCCCAGATCGTAAGCTCATCCTTGCCGTGAATGGTGAAATCTATAAC
CACAAAGAAATTCGTGAACGTTACAAGGGTCAATATGAATTCCAAACCGATTCTGATTGTGAAGTTATCTTGGCACTATA
CCGCGATAAAGGAGCTGATCTCCTCGAAGAACTCAATGGTATTTTTGCTTTTGTTCTCTACGATGAAGAGAAAGATGAAT
ACCTGATTGGACGTGACCATATCGGCATCATTCCTCTTTATCAGGGTCATGATGAACACGGTAACTTCTATGTAGCGTCA
GAGATGAAAGCACTGGTTCCTGTGTGTAAAACATTGAGTGAGTTTCCTCCTGGCAGCTACTTGAGTTCAAAAGATCAAGC
GGTGACACGCTACTATGTGCGTGATTGGAATAGTTTTGATGAAGTCAAAGATAACGTCAGCAGCAAAGAAGAGTTAACTC
AAGCCTTAGAAGCTGCCGTGAAACGCCAACTGATGACCGATGTTCCTTATGGCGTACTGCTATCTGGTGGCCTGGATTCA
TCGATTACCTCTGCGATTGCGAAGCGTTTTGCCGCAATGCGTATTGAAGATGATGAGAAAAGTGCGGCATGGTGGCCACA
ACTTCACTCTTTTGCGATCGGCTTAGAGGGGGCTCCAGATCTTAAAGCCGCACGTGAGGTTGCAGAGAAAATTGGTACCG
TACACCACGAAATGACTTACACCATTCAAGAGGGACTTGATGCGATTCGTGATGTGATTTACCACATCGAAACTTATGAT
GTCACTACGATTCGTGCTTCTACTCCCATGTTCTTGATGGGGCGTAAAATCAAAGCCATGGGCATCAAAATGGTGCTTTC
CGGTGAGGGAGCAGACGAAATTTTCGGTGGCTATCTCTACTTCCATAAAGCGCCAAACGCTAAAGAGTTTCACGAGGAAA
CCGTACGCAAACTGCTGGCGTTGAACTTATTTGACTGTGCGCGTGCCAACAAATCGCTGGCAGCATGGGGGGTAGAAGGT
CGTGTGCCTTTCCTAGATAAAGAATTTATTGATGTTGCGATGCGCCTCAACCCAGCCGATAAAATGTGTGGTAATGGCAA
AATGGAAAAACACATCCTGCGTGAGTGCTTTGAGCACTATCTGCCCGAGTCGATTGCATGGCGTCAAAAGGAGCAGTTTT
CTGATGGCGTAGGTTATGGCTGGATCGATACGCTAAAAGCTACCGCAGAAGCGAAGATCAGTGATCAACAAATGGAAACT
GCGAAATTCCGTTTCCCATACAATACTCCAACCACCAAAGAAGGTTATGTGTACCGGGAAATTTTTGAGGAACTATTCCC
ACTTGAATCTGCGGCACGCTGCGTACCGGGAGGCCCTTCCGTCGCTTGCTCTTCAGCTAAAGCGATTGAGTGGGATGAGT
CATTCAAAAACTGTATTGACCCATCAGGCCGCGCGGTGAAAGCCGTCCACAAACAAGCCTACTAA

Upstream 100 bases:

>100_bases
AATCTTTTTATTTAAAATAAAACTCAACATGGGGTTGAAGTTTTTAATCAGAATGGCAAATTAAGTCCAACAGATTAAAA
AACATTTAAAAGGAATTCAT

Downstream 100 bases:

>100_bases
GACTTGAAGTTCTAGCCAGCAGCGCTGCTGCTTCAAGTAGGGCGAGTATAGTGATTAAAAAAGCCAGAGTGATGACTCAT
TCTGGCTTTTAATTCTTTCA

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MCSIFGILDIKTDAATLRPIALEMSKKLRHRGPDWSGIYASDKAILAHERLAIVGLNSGAQPLYSPDRKLILAVNGEIYN
HKEIRERYKGQYEFQTDSDCEVILALYRDKGADLLEELNGIFAFVLYDEEKDEYLIGRDHIGIIPLYQGHDEHGNFYVAS
EMKALVPVCKTLSEFPPGSYLSSKDQAVTRYYVRDWNSFDEVKDNVSSKEELTQALEAAVKRQLMTDVPYGVLLSGGLDS
SITSAIAKRFAAMRIEDDEKSAAWWPQLHSFAIGLEGAPDLKAAREVAEKIGTVHHEMTYTIQEGLDAIRDVIYHIETYD
VTTIRASTPMFLMGRKIKAMGIKMVLSGEGADEIFGGYLYFHKAPNAKEFHEETVRKLLALNLFDCARANKSLAAWGVEG
RVPFLDKEFIDVAMRLNPADKMCGNGKMEKHILRECFEHYLPESIAWRQKEQFSDGVGYGWIDTLKATAEAKISDQQMET
AKFRFPYNTPTTKEGYVYREIFEELFPLESAARCVPGGPSVACSSAKAIEWDESFKNCIDPSGRAVKAVHKQAY

Sequences:

>Translated_554_residues
MCSIFGILDIKTDAATLRPIALEMSKKLRHRGPDWSGIYASDKAILAHERLAIVGLNSGAQPLYSPDRKLILAVNGEIYN
HKEIRERYKGQYEFQTDSDCEVILALYRDKGADLLEELNGIFAFVLYDEEKDEYLIGRDHIGIIPLYQGHDEHGNFYVAS
EMKALVPVCKTLSEFPPGSYLSSKDQAVTRYYVRDWNSFDEVKDNVSSKEELTQALEAAVKRQLMTDVPYGVLLSGGLDS
SITSAIAKRFAAMRIEDDEKSAAWWPQLHSFAIGLEGAPDLKAAREVAEKIGTVHHEMTYTIQEGLDAIRDVIYHIETYD
VTTIRASTPMFLMGRKIKAMGIKMVLSGEGADEIFGGYLYFHKAPNAKEFHEETVRKLLALNLFDCARANKSLAAWGVEG
RVPFLDKEFIDVAMRLNPADKMCGNGKMEKHILRECFEHYLPESIAWRQKEQFSDGVGYGWIDTLKATAEAKISDQQMET
AKFRFPYNTPTTKEGYVYREIFEELFPLESAARCVPGGPSVACSSAKAIEWDESFKNCIDPSGRAVKAVHKQAY
>Mature_554_residues
MCSIFGILDIKTDAATLRPIALEMSKKLRHRGPDWSGIYASDKAILAHERLAIVGLNSGAQPLYSPDRKLILAVNGEIYN
HKEIRERYKGQYEFQTDSDCEVILALYRDKGADLLEELNGIFAFVLYDEEKDEYLIGRDHIGIIPLYQGHDEHGNFYVAS
EMKALVPVCKTLSEFPPGSYLSSKDQAVTRYYVRDWNSFDEVKDNVSSKEELTQALEAAVKRQLMTDVPYGVLLSGGLDS
SITSAIAKRFAAMRIEDDEKSAAWWPQLHSFAIGLEGAPDLKAAREVAEKIGTVHHEMTYTIQEGLDAIRDVIYHIETYD
VTTIRASTPMFLMGRKIKAMGIKMVLSGEGADEIFGGYLYFHKAPNAKEFHEETVRKLLALNLFDCARANKSLAAWGVEG
RVPFLDKEFIDVAMRLNPADKMCGNGKMEKHILRECFEHYLPESIAWRQKEQFSDGVGYGWIDTLKATAEAKISDQQMET
AKFRFPYNTPTTKEGYVYREIFEELFPLESAARCVPGGPSVACSSAKAIEWDESFKNCIDPSGRAVKAVHKQAY

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=545, Percent_Identity=38.8990825688073, Blast_Score=317, Evalue=2e-86,
Organism=Homo sapiens, GI168229252, Length=545, Percent_Identity=38.8990825688073, Blast_Score=317, Evalue=2e-86,
Organism=Homo sapiens, GI168229250, Length=545, Percent_Identity=38.8990825688073, Blast_Score=317, Evalue=2e-86,
Organism=Homo sapiens, GI296010848, Length=519, Percent_Identity=40.0770712909441, Blast_Score=316, Evalue=3e-86,
Organism=Homo sapiens, GI296010852, Length=490, Percent_Identity=39.3877551020408, Blast_Score=285, Evalue=5e-77,
Organism=Homo sapiens, GI296010850, Length=490, Percent_Identity=39.3877551020408, Blast_Score=285, Evalue=5e-77,
Organism=Escherichia coli, GI1786889, Length=554, Percent_Identity=75.6317689530686, Blast_Score=905, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147557, Length=563, Percent_Identity=43.6944937833037, Blast_Score=423, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI71993933, Length=561, Percent_Identity=43.49376114082, Blast_Score=419, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17560178, Length=519, Percent_Identity=35.645472061657, Blast_Score=288, Evalue=7e-78,
Organism=Caenorhabditis elegans, GI25147560, Length=313, Percent_Identity=39.297124600639, Blast_Score=189, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6321563, Length=545, Percent_Identity=51.9266055045872, Blast_Score=541, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6325403, Length=546, Percent_Identity=51.2820512820513, Blast_Score=536, Evalue=1e-153,
Organism=Drosophila melanogaster, GI45553209, Length=577, Percent_Identity=46.6204506065858, Blast_Score=482, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 62363; Mature: 62363

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSIFGILDIKTDAATLRPIALEMSKKLRHRGPDWSGIYASDKAILAHERLAIVGLNSGA
CCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEECCEEEEECCCCC
QPLYSPDRKLILAVNGEIYNHKEIRERYKGQYEFQTDSDCEVILALYRDKGADLLEELNG
CCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEECCCCCHHHHHEEHHCCCHHHHHHCCC
IFAFVLYDEEKDEYLIGRDHIGIIPLYQGHDEHGNFYVASEMKALVPVCKTLSEFPPGSY
EEEEEEEECCCCCEEECCCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCC
LSSKDQAVTRYYVRDWNSFDEVKDNVSSKEELTQALEAAVKRQLMTDVPYGVLLSGGLDS
CCCCHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SITSAIAKRFAAMRIEDDEKSAAWWPQLHSFAIGLEGAPDLKAAREVAEKIGTVHHEMTY
HHHHHHHHHHHHHEECCCCCCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHH
TIQEGLDAIRDVIYHIETYDVTTIRASTPMFLMGRKIKAMGIKMVLSGEGADEIFGGYLY
HHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHCCEEHCCCEEEEEECCCCHHHHCCEEE
FHKAPNAKEFHEETVRKLLALNLFDCARANKSLAAWGVEGRVPFLDKEFIDVAMRLNPAD
EECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHCCCHH
KMCGNGKMEKHILRECFEHYLPESIAWRQKEQFSDGVGYGWIDTLKATAEAKISDQQMET
HHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHH
AKFRFPYNTPTTKEGYVYREIFEELFPLESAARCVPGGPSVACSSAKAIEWDESFKNCID
HEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCC
PSGRAVKAVHKQAY
CCCHHHHHHHHHCC
>Mature Secondary Structure
MCSIFGILDIKTDAATLRPIALEMSKKLRHRGPDWSGIYASDKAILAHERLAIVGLNSGA
CCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEECCEEEEECCCCC
QPLYSPDRKLILAVNGEIYNHKEIRERYKGQYEFQTDSDCEVILALYRDKGADLLEELNG
CCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEECCCCCHHHHHEEHHCCCHHHHHHCCC
IFAFVLYDEEKDEYLIGRDHIGIIPLYQGHDEHGNFYVASEMKALVPVCKTLSEFPPGSY
EEEEEEEECCCCCEEECCCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCC
LSSKDQAVTRYYVRDWNSFDEVKDNVSSKEELTQALEAAVKRQLMTDVPYGVLLSGGLDS
CCCCHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SITSAIAKRFAAMRIEDDEKSAAWWPQLHSFAIGLEGAPDLKAAREVAEKIGTVHHEMTY
HHHHHHHHHHHHHEECCCCCCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHH
TIQEGLDAIRDVIYHIETYDVTTIRASTPMFLMGRKIKAMGIKMVLSGEGADEIFGGYLY
HHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHCCEEHCCCEEEEEECCCCHHHHCCEEE
FHKAPNAKEFHEETVRKLLALNLFDCARANKSLAAWGVEGRVPFLDKEFIDVAMRLNPAD
EECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHCCCHH
KMCGNGKMEKHILRECFEHYLPESIAWRQKEQFSDGVGYGWIDTLKATAEAKISDQQMET
HHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHH
AKFRFPYNTPTTKEGYVYREIFEELFPLESAARCVPGGPSVACSSAKAIEWDESFKNCID
HEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCC
PSGRAVKAVHKQAY
CCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]