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The map label for this gene is holA [H]

Identifier: 227081127

GI number: 227081127

Start: 974418

End: 975446

Strand: Reverse

Name: holA [H]

Synonym: VCM66_0909

Alternate gene names: 227081127

Gene position: 975446-974418 (Counterclockwise)

Preceding gene: 227081128

Following gene: 227081126

Centisome position: 33.72

GC content: 49.47

Gene sequence:

>1029_bases
ATGCGCATTTACGCTGAAAAACTGGCTGAATCACTGCACAAAACGCTCTATCCGATTTACCTCGTATTCGGTAATGAGCC
ACTGCTGCTCCAAGAAGCCAAAACCGCCATCGAAAAAACGGCACAAGCGCAAGGTTTTTTGGAAAAACACCGATTTAGCG
CGGATGCCGGGCTCGATTGGAACGCGGTGTATGACTGTTGCCAAGCACTGAGTCTGTTTTCATCTCGCCAACTTATCGAA
ATTGAGATCCCAGAATCTGGGGTCAATGCTCAAACGGCAAAGGAACTTAGCGCACTGGTAGGCCAACTTCACCAAGACAT
TCTCTTGCTGGTGATTGGCCCCAAACTGACTAAGGCGCAAGAGAACGCAGCTTGGTTCAAAACGCTTGCGCAGCAAGCCT
GTTGGGTTAACTGCCTCACGCCAGAATTGAGCCGTTTACCTCAGTTTGTTCAGCAGCGCTGTTTTGCCCTCGGTTTAAAA
CCGGATGCCGAAGCGGTACAAATGTTGGCGCAGTGGCATGAAGGTAATCTCTTTGCATTAGCCCAGAGTCTCGAAAAATT
AGCGTTACTCTACCCCGATGGTCTACTGACTTTAGTGCGCTTGGAAGAGTCGCTGAGCCGCCATAACCATTTCACGCCTT
ATCACTGGATGGATGCGCTATTAGAAGGCAAAGCCAACCGTGCTCAGCGTATTTTGCGCCAGCTTATGCTCGAAGAGAGT
GAGCCGATTATTCTGATCCGCACCGCGCAAAAAGAGCTGACCCAACTGCTTAAATGGCAACAAGAGCGCCAACAGCTGGG
TAATTTGGGGAGTCTTTTTGATCGCTATCGTGTCTGGCAGAATCGAAGACCACTCTACTCTGCCGCTTTACAGCGCTTAC
CAAGTCGCGCCCTACTTCGGTTGGTTGGAATATTAACTCAGGCTGAATTACTGGCAAAAACGCAATACGAACAGCCAGTA
TGGCCAATCTTGCAGCAACTAAGCCTTGAATGCTGCAATCCTCAGGCAAACCTACCTTTGCCTCACTAA

Upstream 100 bases:

>100_bases
ATGATGAAAGTATTGAAGGCAAAGGGATCACTGACTATCGCGTTGATACGCAAGAAGTAGCCAACCCAGTGTCAACAGAA
GGCAGCAGTGAGTAGTTTCG

Downstream 100 bases:

>100_bases
AAGCGTGATATACTGCGCCCTTTTAAGCGAAAGCTGTATTTTATTGATGATTTTCAGAGGACATTCTCTTGCAAGGTGAA
GCACTTAAAGACTTTCTTTT

Product: DNA polymerase III subunit delta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MRIYAEKLAESLHKTLYPIYLVFGNEPLLLQEAKTAIEKTAQAQGFLEKHRFSADAGLDWNAVYDCCQALSLFSSRQLIE
IEIPESGVNAQTAKELSALVGQLHQDILLLVIGPKLTKAQENAAWFKTLAQQACWVNCLTPELSRLPQFVQQRCFALGLK
PDAEAVQMLAQWHEGNLFALAQSLEKLALLYPDGLLTLVRLEESLSRHNHFTPYHWMDALLEGKANRAQRILRQLMLEES
EPIILIRTAQKELTQLLKWQQERQQLGNLGSLFDRYRVWQNRRPLYSAALQRLPSRALLRLVGILTQAELLAKTQYEQPV
WPILQQLSLECCNPQANLPLPH

Sequences:

>Translated_342_residues
MRIYAEKLAESLHKTLYPIYLVFGNEPLLLQEAKTAIEKTAQAQGFLEKHRFSADAGLDWNAVYDCCQALSLFSSRQLIE
IEIPESGVNAQTAKELSALVGQLHQDILLLVIGPKLTKAQENAAWFKTLAQQACWVNCLTPELSRLPQFVQQRCFALGLK
PDAEAVQMLAQWHEGNLFALAQSLEKLALLYPDGLLTLVRLEESLSRHNHFTPYHWMDALLEGKANRAQRILRQLMLEES
EPIILIRTAQKELTQLLKWQQERQQLGNLGSLFDRYRVWQNRRPLYSAALQRLPSRALLRLVGILTQAELLAKTQYEQPV
WPILQQLSLECCNPQANLPLPH
>Mature_342_residues
MRIYAEKLAESLHKTLYPIYLVFGNEPLLLQEAKTAIEKTAQAQGFLEKHRFSADAGLDWNAVYDCCQALSLFSSRQLIE
IEIPESGVNAQTAKELSALVGQLHQDILLLVIGPKLTKAQENAAWFKTLAQQACWVNCLTPELSRLPQFVQQRCFALGLK
PDAEAVQMLAQWHEGNLFALAQSLEKLALLYPDGLLTLVRLEESLSRHNHFTPYHWMDALLEGKANRAQRILRQLMLEES
EPIILIRTAQKELTQLLKWQQERQQLGNLGSLFDRYRVWQNRRPLYSAALQRLPSRALLRLVGILTQAELLAKTQYEQPV
WPILQQLSLECCNPQANLPLPH

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The delta subunit seems to interact with the gamma subunit to transfer the b

COG id: COG1466

COG function: function code L; DNA polymerase III, delta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786859, Length=333, Percent_Identity=49.8498498498498, Blast_Score=291, Evalue=4e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR010372
- InterPro:   IPR005790 [H]

Pfam domain/function: PF06144 DNA_pol3_delta [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 39049; Mature: 39049

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIYAEKLAESLHKTLYPIYLVFGNEPLLLQEAKTAIEKTAQAQGFLEKHRFSADAGLDW
CCCHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
NAVYDCCQALSLFSSRQLIEIEIPESGVNAQTAKELSALVGQLHQDILLLVIGPKLTKAQ
HHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCHHHHH
ENAAWFKTLAQQACWVNCLTPELSRLPQFVQQRCFALGLKPDAEAVQMLAQWHEGNLFAL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHH
AQSLEKLALLYPDGLLTLVRLEESLSRHNHFTPYHWMDALLEGKANRAQRILRQLMLEES
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCC
EPIILIRTAQKELTQLLKWQQERQQLGNLGSLFDRYRVWQNRRPLYSAALQRLPSRALLR
CCEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHH
LVGILTQAELLAKTQYEQPVWPILQQLSLECCNPQANLPLPH
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRIYAEKLAESLHKTLYPIYLVFGNEPLLLQEAKTAIEKTAQAQGFLEKHRFSADAGLDW
CCCHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
NAVYDCCQALSLFSSRQLIEIEIPESGVNAQTAKELSALVGQLHQDILLLVIGPKLTKAQ
HHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCHHHHH
ENAAWFKTLAQQACWVNCLTPELSRLPQFVQQRCFALGLKPDAEAVQMLAQWHEGNLFAL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHH
AQSLEKLALLYPDGLLTLVRLEESLSRHNHFTPYHWMDALLEGKANRAQRILRQLMLEES
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCC
EPIILIRTAQKELTQLLKWQQERQQLGNLGSLFDRYRVWQNRRPLYSAALQRLPSRALLR
CCEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHH
LVGILTQAELLAKTQYEQPVWPILQQLSLECCNPQANLPLPH
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8505303; 1400251; 8905232; 9278503; 3316191; 8505304; 1575709 [H]