Click here to switch to the map view.

The map label for this gene is queF [H]

Identifier: 227081077

GI number: 227081077

Start: 920207

End: 921070

Strand: Reverse

Name: queF [H]

Synonym: VCM66_0859

Alternate gene names: 227081077

Gene position: 921070-920207 (Counterclockwise)

Preceding gene: 227081080

Following gene: 227081076

Centisome position: 31.84

GC content: 49.07

Gene sequence:

>864_bases
TTGAATCGACTGAAAAACATGAGCAAATATTCCGATGCCAAAGAGCTGGCAAGCTTAACTCTGGGTAAAAAAACCGAGTA
TGCCAACCAGTATGACCCAAGCTTACTGCAACCTGTGCCACGCAGCTTGAACCGCAATGATCTGCATCTTAGCGCGACTC
TGCCTTTTCAAGGCTGCGATATTTGGACGCTGTATGAGCTCTCTTGGCTCAATCAGAAAGGCCTGCCCCAAGTGGCGATC
GGAGAAGTGTCGATTCCAGCGACAAGTGCCAATTTGATCGAATCCAAATCGTTCAAGCTCTATCTCAACAGCTACAATCA
GACTCGTTTTGCGTCTTGGGATGAAGTGCAAACGCGCTTAGTGCACGATCTTTCGGCCTGCGCCGGTGAAACCGTCACCG
TTAACGTAAAGTCTCTGAACGAATACACCGCAGAGCCGATAGTGACCATGCAAGGCGAGTGCATTGATGATCAAGATATT
GAAATCGCTAACTACGAGTTTGACGATGCCTTGCTGCAAGGTGCAGCACAGGGTGAAGAAGTCAGCGAAGTACTGCACAG
CCATTTGCTAAAATCCAACTGCCTAATCACCAATCAACCCGACTGGGGGAGCGTCGAAATTGCGTATCACGGCGCTAAGA
TGAATCGTGAAGCCTTACTGCGTTATCTTGTCTCTTTCCGTGAGCATAATGAGTTTCATGAGCAGTGTGTTGAGCGTATT
TTCACCGACATTATGCGCTACTGCCAGCCACAGAGTTTGACCGTGTATGCGCGTTATACTCGACGCGGTGGCTTAGACAT
TAACCCATTCCGCTCCAGCCACCAGTCAGCACCGAACCATAATCAACGCATGGCGCGACAATAA

Upstream 100 bases:

>100_bases
CTTGCACTACGCTTTGCGTCATAATCATCTCCATGTTGAGTTTGCGCCGCCTCGTTAATGACACTGGCACTGGGATTGCG
TAAAATTCTGCACATTCTAA

Downstream 100 bases:

>100_bases
GGATAAGACTTAGGATGAGATTTCTGCGCTGGATAACCATAGCGACCTTACTTCTGGCTTCAAGTTGGGCTCAGGCCACG
ATTTACTCTTCACCTATGCT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MNRLKNMSKYSDAKELASLTLGKKTEYANQYDPSLLQPVPRSLNRNDLHLSATLPFQGCDIWTLYELSWLNQKGLPQVAI
GEVSIPATSANLIESKSFKLYLNSYNQTRFASWDEVQTRLVHDLSACAGETVTVNVKSLNEYTAEPIVTMQGECIDDQDI
EIANYEFDDALLQGAAQGEEVSEVLHSHLLKSNCLITNQPDWGSVEIAYHGAKMNREALLRYLVSFREHNEFHEQCVERI
FTDIMRYCQPQSLTVYARYTRRGGLDINPFRSSHQSAPNHNQRMARQ

Sequences:

>Translated_287_residues
MNRLKNMSKYSDAKELASLTLGKKTEYANQYDPSLLQPVPRSLNRNDLHLSATLPFQGCDIWTLYELSWLNQKGLPQVAI
GEVSIPATSANLIESKSFKLYLNSYNQTRFASWDEVQTRLVHDLSACAGETVTVNVKSLNEYTAEPIVTMQGECIDDQDI
EIANYEFDDALLQGAAQGEEVSEVLHSHLLKSNCLITNQPDWGSVEIAYHGAKMNREALLRYLVSFREHNEFHEQCVERI
FTDIMRYCQPQSLTVYARYTRRGGLDINPFRSSHQSAPNHNQRMARQ
>Mature_287_residues
MNRLKNMSKYSDAKELASLTLGKKTEYANQYDPSLLQPVPRSLNRNDLHLSATLPFQGCDIWTLYELSWLNQKGLPQVAI
GEVSIPATSANLIESKSFKLYLNSYNQTRFASWDEVQTRLVHDLSACAGETVTVNVKSLNEYTAEPIVTMQGECIDDQDI
EIANYEFDDALLQGAAQGEEVSEVLHSHLLKSNCLITNQPDWGSVEIAYHGAKMNREALLRYLVSFREHNEFHEQCVERI
FTDIMRYCQPQSLTVYARYTRRGGLDINPFRSSHQSAPNHNQRMARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=283, Percent_Identity=63.6042402826855, Blast_Score=365, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 32726; Mature: 32726

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRLKNMSKYSDAKELASLTLGKKTEYANQYDPSLLQPVPRSLNRNDLHLSATLPFQGCD
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCC
IWTLYELSWLNQKGLPQVAIGEVSIPATSANLIESKSFKLYLNSYNQTRFASWDEVQTRL
EEEEEEEHHCCCCCCCCEEECCEECCCCCHHHHHCCCEEEEEECCCCCCCCCHHHHHHHH
VHDLSACAGETVTVNVKSLNEYTAEPIVTMQGECIDDQDIEIANYEFDDALLQGAAQGEE
HHHHHHHCCCEEEEEEECCHHHCCCCEEEECCCCCCCCCCEEECCCHHHHHHHCCCCCHH
VSEVLHSHLLKSNCLITNQPDWGSVEIAYHGAKMNREALLRYLVSFREHNEFHEQCVERI
HHHHHHHHHHHCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHH
FTDIMRYCQPQSLTVYARYTRRGGLDINPFRSSHQSAPNHNQRMARQ
HHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCC
>Mature Secondary Structure
MNRLKNMSKYSDAKELASLTLGKKTEYANQYDPSLLQPVPRSLNRNDLHLSATLPFQGCD
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCC
IWTLYELSWLNQKGLPQVAIGEVSIPATSANLIESKSFKLYLNSYNQTRFASWDEVQTRL
EEEEEEEHHCCCCCCCCEEECCEECCCCCHHHHHCCCEEEEEECCCCCCCCCHHHHHHHH
VHDLSACAGETVTVNVKSLNEYTAEPIVTMQGECIDDQDIEIANYEFDDALLQGAAQGEE
HHHHHHHCCCEEEEEEECCHHHCCCCEEEECCCCCCCCCCEEECCCHHHHHHHCCCCCHH
VSEVLHSHLLKSNCLITNQPDWGSVEIAYHGAKMNREALLRYLVSFREHNEFHEQCVERI
HHHHHHHHHHHCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHH
FTDIMRYCQPQSLTVYARYTRRGGLDINPFRSSHQSAPNHNQRMARQ
HHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]