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The map label for this gene is mltF

Identifier: 227081042

GI number: 227081042

Start: 887399

End: 888991

Strand: Reverse

Name: mltF

Synonym: VCM66_0823

Alternate gene names: 227081042

Gene position: 888991-887399 (Counterclockwise)

Preceding gene: 227081043

Following gene: 227081041

Centisome position: 30.73

GC content: 48.4

Gene sequence:

>1593_bases
ATGACTCCTTTTGCCTATAAACTCCCCATTCGCGCACTCTGGCTAGGCTTATTAAGCCTGCTCTTAGTCGGTTGCCAAAT
CGATTCCGAGCCCAAAAGCGAGCTAGAAAAAATTCGTGAACGTGGTGTTTTACGCGTTGGTACGCTCAACAATCCCCTCT
CCTACTACATCGGCCCTGATGGCCCAACGGGATTGGATTACGAGTTAGCCCGCGAATTTGCCAAAGAGCTTGGCGTAAAG
CTGGAAATGAAGCCAGCATACCGCCTATCTAGCCTGTTTCCTGCCTTAAAAAACGGTGAAGTGGACATCATTGCCGCAGG
CTTAAGCCAATCCGAAGAGCGACTCAAAGATTTTCGCGCAGGTCCTGCTTATTATTACGTTTCGCAGCAAGTGGTCTATA
AAAAAGGTGACTGGCGACCACGCAGCTTCAAACAGTTGGTCGAACGCCAGCAAACCTTATTAAAGGACAATCCTGAGCTC
GCCTTTTTCAGCGTGGTGGATGACTCCCATTTTGAGCACACCTTGCTAGCCAAACAGCAGAAATACCCCGATTTTCAATT
TCATGTCGACAGCAATTCCGATGTGAATGACTTACTGAAAAAAGTCTCACAAGGAGAGTTACTGTTTACGATGGCCGACT
CTGTTGAGGTGTCGCTTTCTCAACGCATCTATCCTGAACTGGCGGCGGCATTTGAATTAACCGAAGATCAGCCGATCTCA
TGGTTTATCCGCCGTTCCGACGATGAAAGTCTGTATGCCTTAATGATTGAATTCTTTGGCAATCTCAAGCAATCGGGCTA
TTTGGCGTCTTTGGAAGAAAAATACATCGGCCATATTGGTGCCTTTGATTATGTCGATACCCGCGCCTTTATTCGCGCGC
TGGATACACGATTACCTCGATGGACGCCTCTCTTTCAAAAGTACTCGGCAGAGTTTGATTGGCGTTTAGTCGCCGCACTT
GCGTATCAGGAGTCGCACTGGAATCCGTATGCCAAATCGCCCACGGGTGTCCGTGGTTTGATGATGTTGACACTACCAAC
CGCGCGCAGTGTAGGGGTAAGCGATCGCCTCGATCCAGAACAGTCGATTCGTGGTGGGGTGGAATACTTGCGACGTATGA
TGGAGCGTGTCCCTGACTCGATTTCCGAACATGAAAAAATTTGGTTTGCCTTAGCCTCTTACAACATTGGCTACGGGCAT
ATGATGGATGCGCGACGCTTAACCAAAAGCCAAGGCGCAGATCCTGACTCATGGGCGGATGTCAAAGATCGCCTACCACA
GCTACAGCAGAAAAAATATTTCACCCAGACCCGCTATGGCTATGCGCGTGGCGACGAAGCACGTAATTATGTGGAAAATA
TTCGCCGTTACTATCAAAGCATCATCGGCCATCTTGAGCAGCGTCAATTGGCGACAGGCGATGAAAGTATTGAAGATCTC
ACCGTGATTGCGCTTGATGAAGAATTTTTCAATGAAGAAGCAAACCGTGAAATCTTGGATGAAGAAGCGGAAGCACTCGA
ATCAGAGTCACTCGAAAACAGTGAATCGAGCGCCGAGCCATCTGCCAAACCATCCACTGAGAGCAAAAATTAG

Upstream 100 bases:

>100_bases
AAATCTTCGAGCAAAATGACAGCTTACTCAGTGAGGACTTTGCCAAAACCAGCGCCTTTGATATAAAGGCATCTGTCTTG
ATGAGAGAAAACCCAAATTG

Downstream 100 bases:

>100_bases
CCACTTGATTTTTGGGAAGAAAACACGTAAATCAATGTGTGAATTAAATCGCTTTCCTGTTGTCAGAAACAGGTAACATT
CCAATGCAATAATAAAGCAG

Product: putative transglycosylase

Products: NA

Alternate protein names: Murein lyase F

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MTPFAYKLPIRALWLGLLSLLLVGCQIDSEPKSELEKIRERGVLRVGTLNNPLSYYIGPDGPTGLDYELAREFAKELGVK
LEMKPAYRLSSLFPALKNGEVDIIAAGLSQSEERLKDFRAGPAYYYVSQQVVYKKGDWRPRSFKQLVERQQTLLKDNPEL
AFFSVVDDSHFEHTLLAKQQKYPDFQFHVDSNSDVNDLLKKVSQGELLFTMADSVEVSLSQRIYPELAAAFELTEDQPIS
WFIRRSDDESLYALMIEFFGNLKQSGYLASLEEKYIGHIGAFDYVDTRAFIRALDTRLPRWTPLFQKYSAEFDWRLVAAL
AYQESHWNPYAKSPTGVRGLMMLTLPTARSVGVSDRLDPEQSIRGGVEYLRRMMERVPDSISEHEKIWFALASYNIGYGH
MMDARRLTKSQGADPDSWADVKDRLPQLQQKKYFTQTRYGYARGDEARNYVENIRRYYQSIIGHLEQRQLATGDESIEDL
TVIALDEEFFNEEANREILDEEAEALESESLENSESSAEPSAKPSTESKN

Sequences:

>Translated_530_residues
MTPFAYKLPIRALWLGLLSLLLVGCQIDSEPKSELEKIRERGVLRVGTLNNPLSYYIGPDGPTGLDYELAREFAKELGVK
LEMKPAYRLSSLFPALKNGEVDIIAAGLSQSEERLKDFRAGPAYYYVSQQVVYKKGDWRPRSFKQLVERQQTLLKDNPEL
AFFSVVDDSHFEHTLLAKQQKYPDFQFHVDSNSDVNDLLKKVSQGELLFTMADSVEVSLSQRIYPELAAAFELTEDQPIS
WFIRRSDDESLYALMIEFFGNLKQSGYLASLEEKYIGHIGAFDYVDTRAFIRALDTRLPRWTPLFQKYSAEFDWRLVAAL
AYQESHWNPYAKSPTGVRGLMMLTLPTARSVGVSDRLDPEQSIRGGVEYLRRMMERVPDSISEHEKIWFALASYNIGYGH
MMDARRLTKSQGADPDSWADVKDRLPQLQQKKYFTQTRYGYARGDEARNYVENIRRYYQSIIGHLEQRQLATGDESIEDL
TVIALDEEFFNEEANREILDEEAEALESESLENSESSAEPSAKPSTESKN
>Mature_529_residues
TPFAYKLPIRALWLGLLSLLLVGCQIDSEPKSELEKIRERGVLRVGTLNNPLSYYIGPDGPTGLDYELAREFAKELGVKL
EMKPAYRLSSLFPALKNGEVDIIAAGLSQSEERLKDFRAGPAYYYVSQQVVYKKGDWRPRSFKQLVERQQTLLKDNPELA
FFSVVDDSHFEHTLLAKQQKYPDFQFHVDSNSDVNDLLKKVSQGELLFTMADSVEVSLSQRIYPELAAAFELTEDQPISW
FIRRSDDESLYALMIEFFGNLKQSGYLASLEEKYIGHIGAFDYVDTRAFIRALDTRLPRWTPLFQKYSAEFDWRLVAALA
YQESHWNPYAKSPTGVRGLMMLTLPTARSVGVSDRLDPEQSIRGGVEYLRRMMERVPDSISEHEKIWFALASYNIGYGHM
MDARRLTKSQGADPDSWADVKDRLPQLQQKKYFTQTRYGYARGDEARNYVENIRRYYQSIIGHLEQRQLATGDESIEDLT
VIALDEEFFNEEANREILDEEAEALESESLENSESSAEPSAKPSTESKN

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family

Homologues:

Organism=Escherichia coli, GI171474010, Length=468, Percent_Identity=49.5726495726496, Blast_Score=454, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MLTF_VIBC3 (A5F353)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F353
- SMR:   A5F353
- STRING:   A5F353
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0392
- eggNOG:   COG4623
- HOGENOM:   HBG644469
- OMA:   QYVENIR
- ProtClustDB:   PRK10859
- BioCyc:   VCHO345073:VC0395_A0392-MONOMER
- HAMAP:   MF_02016
- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189
- SMART:   SM00062

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT

EC number: NA

Molecular weight: Translated: 60768; Mature: 60637

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00922 TRANSGLYCOSYLASE; PS00013 PROKAR_LIPOPROTEIN

Important sites: ACT_SITE 324-324

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPFAYKLPIRALWLGLLSLLLVGCQIDSEPKSELEKIRERGVLRVGTLNNPLSYYIGPD
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEECCCCCCEEEECCC
GPTGLDYELAREFAKELGVKLEMKPAYRLSSLFPALKNGEVDIIAAGLSQSEERLKDFRA
CCCCCCHHHHHHHHHHCCCEEEECHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCC
GPAYYYVSQQVVYKKGDWRPRSFKQLVERQQTLLKDNPELAFFSVVDDSHFEHTLLAKQQ
CCEEEEECHHHEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHH
KYPDFQFHVDSNSDVNDLLKKVSQGELLFTMADSVEVSLSQRIYPELAAAFELTEDQPIS
CCCCEEEEECCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCE
WFIRRSDDESLYALMIEFFGNLKQSGYLASLEEKYIGHIGAFDYVDTRAFIRALDTRLPR
EEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
WTPLFQKYSAEFDWRLVAALAYQESHWNPYAKSPTGVRGLMMLTLPTARSVGVSDRLDPE
CCHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHH
QSIRGGVEYLRRMMERVPDSISEHEKIWFALASYNIGYGHMMDARRLTKSQGADPDSWAD
HHHHHHHHHHHHHHHHCCHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHH
VKDRLPQLQQKKYFTQTRYGYARGDEARNYVENIRRYYQSIIGHLEQRQLATGDESIEDL
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
TVIALDEEFFNEEANREILDEEAEALESESLENSESSAEPSAKPSTESKN
EEEEECHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TPFAYKLPIRALWLGLLSLLLVGCQIDSEPKSELEKIRERGVLRVGTLNNPLSYYIGPD
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEECCCCCCEEEECCC
GPTGLDYELAREFAKELGVKLEMKPAYRLSSLFPALKNGEVDIIAAGLSQSEERLKDFRA
CCCCCCHHHHHHHHHHCCCEEEECHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCC
GPAYYYVSQQVVYKKGDWRPRSFKQLVERQQTLLKDNPELAFFSVVDDSHFEHTLLAKQQ
CCEEEEECHHHEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHH
KYPDFQFHVDSNSDVNDLLKKVSQGELLFTMADSVEVSLSQRIYPELAAAFELTEDQPIS
CCCCEEEEECCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCE
WFIRRSDDESLYALMIEFFGNLKQSGYLASLEEKYIGHIGAFDYVDTRAFIRALDTRLPR
EEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
WTPLFQKYSAEFDWRLVAALAYQESHWNPYAKSPTGVRGLMMLTLPTARSVGVSDRLDPE
CCHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHH
QSIRGGVEYLRRMMERVPDSISEHEKIWFALASYNIGYGHMMDARRLTKSQGADPDSWAD
HHHHHHHHHHHHHHHHCCHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHH
VKDRLPQLQQKKYFTQTRYGYARGDEARNYVENIRRYYQSIIGHLEQRQLATGDESIEDL
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
TVIALDEEFFNEEANREILDEEAEALESESLENSESSAEPSAKPSTESKN
EEEEECHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA