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The map label for this gene is pepB [H]

Identifier: 227080934

GI number: 227080934

Start: 763877

End: 765211

Strand: Direct

Name: pepB [H]

Synonym: VCM66_0713

Alternate gene names: 227080934

Gene position: 763877-765211 (Clockwise)

Preceding gene: 227080933

Following gene: 227080935

Centisome position: 26.41

GC content: 51.09

Gene sequence:

>1335_bases
ATGAGATGGCACAGGCAAAGCCAATATTTAAGACAAGGAGAAACCATGTCTACACAGATGTCTGTATTTTTGAGTACTCA
AGCTGCCCAGCCTCAGTGGGGAGAGAAAGCGCTCATTTCTTTTGCAGAGCAAGGTGCAACCATTCACCTGCAACAAACAC
AAGATTTCAGCGCGATCCAACGCGCAGCTCGTAAGTTAGATAATCAAGGTATCCGCACGGCATTCCTGACTGGAGAAGGT
TGGGATCTGGAGAGCATTTGGGCTTTCTATCAAGGTTACCGTGATGCGAAAAAGCGCAATACCGTCGAGTGGAAAGCGTT
AGCGGCTGCTGAACAAGCAGAGCTAGAAGCGCGCATTAAAGCGACGGATTGGACACGCGATATCATCAACAAAAGCGCGG
AAGAAGTTGCACCACGCCAATTGGCGACCATGGCAGCAGAGTTCATCAAATCGCTTGCGCCTGACCATGTTTCTTACCGT
ATCGTCAAAGACAAAGATCTGCTCACTGAAGGGTGGGAGGGGATTTACGCTGTAGGCCGTGGCTCTGAGCGTACGTCGGC
GATGCTGCAACTCGACTACAACCCAACGGGCGATGAAAATGCGCCGGTATTCGCGTGTTTAGTCGGTAAAGGCATCACTT
TTGACTCGGGTGGTTACAGCTTAAAACCTTCCAACATGATGTCAGCGATGAAAGCGGACATGGGCGGCTCAGGCATGATC
ACTGGTGCGCTCGGTTTGGCTATCATGCGTGGCTTTAACAAGCGCGTGAAACTCATCCTGTGCTGCGCGGAAAACATGGT
CTCCGGCCGTGCGTTGAAGCTTGGTGACATCATCACCTACAAAAATGGCAAAACCGTTGAAATCATGAACACCGATGCGG
AAGGTCGTTTGGTGCTGGCCGATGGTCTTATCTACGCCAGTGAGCAGAAACCGCAATTGATTATCGACTGTGCAACCTTA
ACCGGAGCGGCGAAAAACGCGCTGGGTAATGATTACCACGCACTGCTTTCTTATGATGAGTCGCTGAGCCAACAAGCATT
AGCTGCGGCAAAAGAAGAGAATGAAGCGCTGTGGGCTCTGCCTTTAGCTGAGTTCCACCGTGAAATGCTGCCTTCTAACT
TTGCGGATCTGTCAAACATCAGTAACGGCGATTACACGCCGGGAGCTAGCACCGCAGCGGCCTTCCTTTCCTATTTCGTG
GAAGGCTACCAAAAAGGTTGGCTACACTTTGACTGTTCAGCCACGTATCGCAAGTCAGCCAGCGATAAATGGGCCGCCGG
AGCGACGGGCATGGGCGTGAAAATGCTCGCACGTATTTTGATGCAGCAAGCATAA

Upstream 100 bases:

>100_bases
CAGAGTTAACACTTCTTAGCAAAAAATGCGGACCACTTGGTCCGTTTTTTTATTCGGTTGACATTTTGCCTCGACATAAT
GCTAACATCTTGGCGATTTA

Downstream 100 bases:

>100_bases
ATTTAAAGGCAGCTTGTATAGCTGCCTTGTTTTTATTAATAAAAGTATACCTTTCCTATTTGACGCTGCAGCGGTGTTGG
CTCTGTTCGTTCATCCTAAT

Product: aminopeptidase B

Products: NA

Alternate protein names: Aminopeptidase B [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MRWHRQSQYLRQGETMSTQMSVFLSTQAAQPQWGEKALISFAEQGATIHLQQTQDFSAIQRAARKLDNQGIRTAFLTGEG
WDLESIWAFYQGYRDAKKRNTVEWKALAAAEQAELEARIKATDWTRDIINKSAEEVAPRQLATMAAEFIKSLAPDHVSYR
IVKDKDLLTEGWEGIYAVGRGSERTSAMLQLDYNPTGDENAPVFACLVGKGITFDSGGYSLKPSNMMSAMKADMGGSGMI
TGALGLAIMRGFNKRVKLILCCAENMVSGRALKLGDIITYKNGKTVEIMNTDAEGRLVLADGLIYASEQKPQLIIDCATL
TGAAKNALGNDYHALLSYDESLSQQALAAAKEENEALWALPLAEFHREMLPSNFADLSNISNGDYTPGASTAAAFLSYFV
EGYQKGWLHFDCSATYRKSASDKWAAGATGMGVKMLARILMQQA

Sequences:

>Translated_444_residues
MRWHRQSQYLRQGETMSTQMSVFLSTQAAQPQWGEKALISFAEQGATIHLQQTQDFSAIQRAARKLDNQGIRTAFLTGEG
WDLESIWAFYQGYRDAKKRNTVEWKALAAAEQAELEARIKATDWTRDIINKSAEEVAPRQLATMAAEFIKSLAPDHVSYR
IVKDKDLLTEGWEGIYAVGRGSERTSAMLQLDYNPTGDENAPVFACLVGKGITFDSGGYSLKPSNMMSAMKADMGGSGMI
TGALGLAIMRGFNKRVKLILCCAENMVSGRALKLGDIITYKNGKTVEIMNTDAEGRLVLADGLIYASEQKPQLIIDCATL
TGAAKNALGNDYHALLSYDESLSQQALAAAKEENEALWALPLAEFHREMLPSNFADLSNISNGDYTPGASTAAAFLSYFV
EGYQKGWLHFDCSATYRKSASDKWAAGATGMGVKMLARILMQQA
>Mature_444_residues
MRWHRQSQYLRQGETMSTQMSVFLSTQAAQPQWGEKALISFAEQGATIHLQQTQDFSAIQRAARKLDNQGIRTAFLTGEG
WDLESIWAFYQGYRDAKKRNTVEWKALAAAEQAELEARIKATDWTRDIINKSAEEVAPRQLATMAAEFIKSLAPDHVSYR
IVKDKDLLTEGWEGIYAVGRGSERTSAMLQLDYNPTGDENAPVFACLVGKGITFDSGGYSLKPSNMMSAMKADMGGSGMI
TGALGLAIMRGFNKRVKLILCCAENMVSGRALKLGDIITYKNGKTVEIMNTDAEGRLVLADGLIYASEQKPQLIIDCATL
TGAAKNALGNDYHALLSYDESLSQQALAAAKEENEALWALPLAEFHREMLPSNFADLSNISNGDYTPGASTAAAFLSYFV
EGYQKGWLHFDCSATYRKSASDKWAAGATGMGVKMLARILMQQA

Specific function: Probably plays an important role in intracellular peptide degradation [H]

COG id: COG0260

COG function: function code E; Leucyl aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M17 family [H]

Homologues:

Organism=Homo sapiens, GI41393561, Length=321, Percent_Identity=35.202492211838, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI47155554, Length=353, Percent_Identity=32.2946175637394, Blast_Score=150, Evalue=3e-36,
Organism=Escherichia coli, GI87082123, Length=425, Percent_Identity=60.2352941176471, Blast_Score=543, Evalue=1e-156,
Organism=Escherichia coli, GI1790710, Length=305, Percent_Identity=39.672131147541, Blast_Score=200, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17556903, Length=259, Percent_Identity=36.6795366795367, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17565172, Length=241, Percent_Identity=29.8755186721992, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21357381, Length=343, Percent_Identity=31.7784256559767, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI221379063, Length=343, Percent_Identity=31.7784256559767, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI221379062, Length=343, Percent_Identity=31.7784256559767, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI21355725, Length=345, Percent_Identity=31.5942028985507, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24661038, Length=345, Percent_Identity=30.7246376811594, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI20129969, Length=372, Percent_Identity=27.9569892473118, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24662227, Length=368, Percent_Identity=26.3586956521739, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI161077148, Length=339, Percent_Identity=27.1386430678466, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI20130057, Length=339, Percent_Identity=27.1386430678466, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19922386, Length=405, Percent_Identity=25.679012345679, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI20129963, Length=368, Percent_Identity=26.9021739130435, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21355645, Length=313, Percent_Identity=25.2396166134185, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24662223, Length=313, Percent_Identity=25.2396166134185, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24646701, Length=211, Percent_Identity=28.9099526066351, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24646703, Length=211, Percent_Identity=28.9099526066351, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21358201, Length=211, Percent_Identity=28.9099526066351, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008330
- InterPro:   IPR011356
- InterPro:   IPR000819 [H]

Pfam domain/function: PF00883 Peptidase_M17 [H]

EC number: =3.4.11.23 [H]

Molecular weight: Translated: 48720; Mature: 48720

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00631 CYTOSOL_AP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWHRQSQYLRQGETMSTQMSVFLSTQAAQPQWGEKALISFAEQGATIHLQQTQDFSAIQ
CCCCHHHHHHHCCCCHHHHEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHH
RAARKLDNQGIRTAFLTGEGWDLESIWAFYQGYRDAKKRNTVEWKALAAAEQAELEARIK
HHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEE
ATDWTRDIINKSAEEVAPRQLATMAAEFIKSLAPDHVSYRIVKDKDLLTEGWEGIYAVGR
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHCCCCEEEECC
GSERTSAMLQLDYNPTGDENAPVFACLVGKGITFDSGGYSLKPSNMMSAMKADMGGSGMI
CCCCCEEEEEEECCCCCCCCCCEEEEEECCCEEECCCCCEECHHHHHHHHHHCCCCCCCH
TGALGLAIMRGFNKRVKLILCCAENMVSGRALKLGDIITYKNGKTVEIMNTDAEGRLVLA
HHHHHHHHHHCCCCCEEEEEEEHHHHCCCCEEEECCEEEECCCCEEEEEECCCCCEEEEE
DGLIYASEQKPQLIIDCATLTGAAKNALGNDYHALLSYDESLSQQALAAAKEENEALWAL
CCEEEECCCCCEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
PLAEFHREMLPSNFADLSNISNGDYTPGASTAAAFLSYFVEGYQKGWLHFDCSATYRKSA
CHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCC
SDKWAAGATGMGVKMLARILMQQA
CCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MRWHRQSQYLRQGETMSTQMSVFLSTQAAQPQWGEKALISFAEQGATIHLQQTQDFSAIQ
CCCCHHHHHHHCCCCHHHHEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHH
RAARKLDNQGIRTAFLTGEGWDLESIWAFYQGYRDAKKRNTVEWKALAAAEQAELEARIK
HHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEE
ATDWTRDIINKSAEEVAPRQLATMAAEFIKSLAPDHVSYRIVKDKDLLTEGWEGIYAVGR
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHCCCCEEEECC
GSERTSAMLQLDYNPTGDENAPVFACLVGKGITFDSGGYSLKPSNMMSAMKADMGGSGMI
CCCCCEEEEEEECCCCCCCCCCEEEEEECCCEEECCCCCEECHHHHHHHHHHCCCCCCCH
TGALGLAIMRGFNKRVKLILCCAENMVSGRALKLGDIITYKNGKTVEIMNTDAEGRLVLA
HHHHHHHHHHCCCCCEEEEEEEHHHHCCCCEEEECCEEEECCCCEEEEEECCCCCEEEEE
DGLIYASEQKPQLIIDCATLTGAAKNALGNDYHALLSYDESLSQQALAAAKEENEALWAL
CCEEEECCCCCEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
PLAEFHREMLPSNFADLSNISNGDYTPGASTAAAFLSYFVEGYQKGWLHFDCSATYRKSA
CHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCC
SDKWAAGATGMGVKMLARILMQQA
CCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]