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The map label for this gene is iscS [H]

Identifier: 227080927

GI number: 227080927

Start: 758651

End: 759865

Strand: Direct

Name: iscS [H]

Synonym: VCM66_0706

Alternate gene names: 227080927

Gene position: 758651-759865 (Clockwise)

Preceding gene: 227080926

Following gene: 227080928

Centisome position: 26.23

GC content: 49.22

Gene sequence:

>1215_bases
ATGAAACTGCCTATTTATCTAGATTATTCAGCGACATGCCCGGTTGATCCGCGCGTAGCTGAAAAAATGGTTCAGTACAT
GACGATGGATGGTACCTTCGGTAACCCAGCTTCGCGCTCGCACCGTTATGGCTGGCAGGCAGAAGAAGCGGTAGACACTG
CGCGTGAGCAAATCGCAGCCCTGCTGAATGCAGACCCACGTGAAATTGTGTTCACTTCGGGTGCTACTGAATCTGACAAC
CTCGCGATCAAAGGTGTTGCTCACTTCTATAACAAGCAAGGTAAGCACATCATCACCAGTAAAACCGAACACAAAGCGGT
ACTGGATACCATGCGCCAATTGGAGCGTGAAGGCTTTGAAGTGACTTATCTTGATCCAGAAAGCAATGGCTTGATTGATC
TGGCTAAGCTTGAAGCCGCGATGCGTGATGACACTATTTTGGTTTCTATCATGCACGTGAACAATGAAATCGGCGTGGTA
CAAGATATCGCAGCGATCGGCGAACTGTGCCGTTCACGTAAAGTGGTGTTCCATGTTGATGCAGCGCAATCCGCGGGTAA
AGTAGCGATTGATGTCCAAGAGATGAAGGTTGATCTGATTTCTCTGTCTGCGCACAAAGCTTACGGCCCGAAAGGTATTG
GTGCTTTGTATGTACGCCGTAAACCACGTATTCGTCTTGAAGCACAAATGCACGGTGGTGGTCATGAACGTGGTTTCCGT
TCTGGTACTCTGCCGACTCACCAAATTGTGGGTATGGGTGAAGCGTTCCGTATCGCGAAGGAAGAGCTGCAGCAAGATTA
CGATCACGCACTGAAGCTGCGTAATCGTCTGCTTGATGGCATTAAAGATATGGAAGCCGTGACCATTAACGGTGATCTGG
ATCAGCGTGTACCACACAATCTCAACGTCAGTTTTGCGTTTGTAGAAGGGGAATCTCTGCTGATGGCACTGAAAGATCTG
GCCGTATCGTCAGGCAGTGCATGTACTTCAGCTAGCCTTGAGCCTTCTTACGTATTGCGCGCTCTGGGTCTTAACGATGA
ACTGGCACACAGCTCAATTCGCTTCTCTTTTGGTCGTTTCACGACCGAGGCAGAGATTGATTATGCGATCGAACTGATCC
GCGTAGCAGTGGATAAACTGCGTGCGATGTCTCCACTTTGGGACATGTACAAAGACGGCGTAGATCTGAACACGGTTGAG
TGGGCACACCACTAA

Upstream 100 bases:

>100_bases
AAAGGGTTTCGCAAAACAACTCAAACACAGTCACCATTGGTGCTGCGCCTTTTGGTATTAATGTTCGCTCATAGCGGCAA
GGTTTACACTGGAGTAGAAA

Downstream 100 bases:

>100_bases
TCATTCCAAGGTTATAAAGGATTCGAGGTAACGAACATGGCATACAGCGAAAAGGTCATTGACCATTACGAGAACCCACG
TAACGTTGGTTCATTTGATA

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKLPIYLDYSATCPVDPRVAEKMVQYMTMDGTFGNPASRSHRYGWQAEEAVDTAREQIAALLNADPREIVFTSGATESDN
LAIKGVAHFYNKQGKHIITSKTEHKAVLDTMRQLEREGFEVTYLDPESNGLIDLAKLEAAMRDDTILVSIMHVNNEIGVV
QDIAAIGELCRSRKVVFHVDAAQSAGKVAIDVQEMKVDLISLSAHKAYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFR
SGTLPTHQIVGMGEAFRIAKEELQQDYDHALKLRNRLLDGIKDMEAVTINGDLDQRVPHNLNVSFAFVEGESLLMALKDL
AVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFGRFTTEAEIDYAIELIRVAVDKLRAMSPLWDMYKDGVDLNTVE
WAHH

Sequences:

>Translated_404_residues
MKLPIYLDYSATCPVDPRVAEKMVQYMTMDGTFGNPASRSHRYGWQAEEAVDTAREQIAALLNADPREIVFTSGATESDN
LAIKGVAHFYNKQGKHIITSKTEHKAVLDTMRQLEREGFEVTYLDPESNGLIDLAKLEAAMRDDTILVSIMHVNNEIGVV
QDIAAIGELCRSRKVVFHVDAAQSAGKVAIDVQEMKVDLISLSAHKAYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFR
SGTLPTHQIVGMGEAFRIAKEELQQDYDHALKLRNRLLDGIKDMEAVTINGDLDQRVPHNLNVSFAFVEGESLLMALKDL
AVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFGRFTTEAEIDYAIELIRVAVDKLRAMSPLWDMYKDGVDLNTVE
WAHH
>Mature_404_residues
MKLPIYLDYSATCPVDPRVAEKMVQYMTMDGTFGNPASRSHRYGWQAEEAVDTAREQIAALLNADPREIVFTSGATESDN
LAIKGVAHFYNKQGKHIITSKTEHKAVLDTMRQLEREGFEVTYLDPESNGLIDLAKLEAAMRDDTILVSIMHVNNEIGVV
QDIAAIGELCRSRKVVFHVDAAQSAGKVAIDVQEMKVDLISLSAHKAYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFR
SGTLPTHQIVGMGEAFRIAKEELQQDYDHALKLRNRLLDGIKDMEAVTINGDLDQRVPHNLNVSFAFVEGESLLMALKDL
AVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFGRFTTEAEIDYAIELIRVAVDKLRAMSPLWDMYKDGVDLNTVE
WAHH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=402, Percent_Identity=58.7064676616915, Blast_Score=512, Evalue=1e-145,
Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=32.3671497584541, Blast_Score=196, Evalue=3e-50,
Organism=Escherichia coli, GI48994898, Length=404, Percent_Identity=79.4554455445545, Blast_Score=685, Evalue=0.0,
Organism=Escherichia coli, GI1789175, Length=397, Percent_Identity=26.1964735516373, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787970, Length=348, Percent_Identity=25.8620689655172, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25143064, Length=403, Percent_Identity=59.3052109181141, Blast_Score=499, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17533177, Length=335, Percent_Identity=34.0298507462687, Blast_Score=165, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=57.6059850374065, Blast_Score=501, Evalue=1e-142,
Organism=Drosophila melanogaster, GI20129463, Length=402, Percent_Identity=58.4577114427861, Blast_Score=489, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 44824; Mature: 44824

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPIYLDYSATCPVDPRVAEKMVQYMTMDGTFGNPASRSHRYGWQAEEAVDTAREQIAA
CCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LLNADPREIVFTSGATESDNLAIKGVAHFYNKQGKHIITSKTEHKAVLDTMRQLEREGFE
HHCCCCCEEEEECCCCCCCCEEEEEHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCCE
VTYLDPESNGLIDLAKLEAAMRDDTILVSIMHVNNEIGVVQDIAAIGELCRSRKVVFHVD
EEEECCCCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEE
AAQSAGKVAIDVQEMKVDLISLSAHKAYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFR
CCCCCCCEEEEHHHHEEEEEEEECCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCC
SGTLPTHQIVGMGEAFRIAKEELQQDYDHALKLRNRLLDGIKDMEAVTINGDLDQRVPHN
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHCCCCC
LNVSFAFVEGESLLMALKDLAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFGRF
CCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHEEECCCC
TTEAEIDYAIELIRVAVDKLRAMSPLWDMYKDGVDLNTVEWAHH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKLPIYLDYSATCPVDPRVAEKMVQYMTMDGTFGNPASRSHRYGWQAEEAVDTAREQIAA
CCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LLNADPREIVFTSGATESDNLAIKGVAHFYNKQGKHIITSKTEHKAVLDTMRQLEREGFE
HHCCCCCEEEEECCCCCCCCEEEEEHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCCE
VTYLDPESNGLIDLAKLEAAMRDDTILVSIMHVNNEIGVVQDIAAIGELCRSRKVVFHVD
EEEECCCCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEE
AAQSAGKVAIDVQEMKVDLISLSAHKAYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFR
CCCCCCCEEEEHHHHEEEEEEEECCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCC
SGTLPTHQIVGMGEAFRIAKEELQQDYDHALKLRNRLLDGIKDMEAVTINGDLDQRVPHN
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHCCCCC
LNVSFAFVEGESLLMALKDLAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFGRF
CCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHEEECCCC
TTEAEIDYAIELIRVAVDKLRAMSPLWDMYKDGVDLNTVEWAHH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA