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The map label for this gene is phoR [H]

Identifier: 227080899

GI number: 227080899

Start: 730428

End: 731729

Strand: Direct

Name: phoR [H]

Synonym: VCM66_0678

Alternate gene names: 227080899

Gene position: 730428-731729 (Clockwise)

Preceding gene: 227080898

Following gene: 227080900

Centisome position: 25.25

GC content: 51.31

Gene sequence:

>1302_bases
ATGGTTGAACGTTTAACATGGAAAAAGCTGGCTTGGGAGCTGGCTTTTTTTTACTTGCCATGGGTTGTGGTGGGATGGAT
TTTCGGAAGTATGCCGTGGCTGCTGTTGGCAGCGACGGCTTTGCAGCTTTTCTGGCATTTGAACCATCAAATTCGCCTTT
CCGCTTGGTTGTGGGATGAAAAACGTCTCACCCCACCTTCTGGAACGGGGAATTGGGAGTCGCTGTTCAATGGCATTTAT
CGCTTGCAGCAGCGCCATCGTAAAAAACGCAAAGAACTGACTAATCTTATTCGCCGTTTCCGTAATGGTGCGGAATCGCT
CCCCGATGCGGTGGTGGTGTTTCGCAGCGAAGGCAATATCGTGTGGTGTAACCGCCTTGCGCAGCATTTGCTGGGTTTTC
GTTGGCCTGACGATGCTGGGCAGCCTATCTCGAACCTGCTGCGTACGCCGGACTTTATCAAATACCTCAAAAAAGATGAT
TTTACTGATCCGCTCGAAATGCGTTCGCCGCTCAATAGTGATCGCATGCTGGAACTGCGGATAGTGCCGTACACCGAAGG
TGAACACCTGATGGTGGTGCGTGATGTCACTCAGCTTAAACAGCTCGAAGGCATGCGCCGTAATTTCTTTGCTAACGTTT
CCCATGAACTGCGTACACCGATGACGGTGCTGCAAGGTTATCTGGAAATGACAGAAGATCCGGATGTGCTCGCGGGCCCG
ATGTGGAGTAAAGCCCATGGTGTGATGACCGAGCAGCTCAACCGGATGAATGGCTTGGTGAACCAACTGCTGACGCTCTC
GAAAATAGAAGCATCACCGATGGTCGAACTCGATCAAGTGGTCGATGTACCAGCCATGTTGGAAGTGTTAGAAAAAGAAG
CAGTGAGCCTTAGTGGTGAGGCGCAGCATAAGCTGCATTTTGAGGTGGATAAGAGCCTTAAGGTGCTGGCGGATGAAGAT
CAGCTGCGCAGCGCGATTTCGAATCTGGTGTATAACGCGGTTAAATATACCCCGCCGGGCGCGCAAATTGATGTGCGTTG
GTATCGCACTGGCAAAGGCGCTTGCCTCGAAGTGGAAGATAAAGGCGAGGGGATCGAGCCGCAACATTTACACCGTTTAA
CGGAGCGTTTCTATCGCGTCGATAAAGCCCGCTCTCGTGATACGGGCGGTAGTGGTTTGGGACTCGCGATCGTGAAACAC
GCGCTTGCACACCATGACACGCATTTGGATATCTACAGTAAAGTCGGTGTCGGCAGTAAGTTTTCGTTTGTTTTACCTAA
GCGGTTGGTGGCGAAAGGATGA

Upstream 100 bases:

>100_bases
AGCCTTAGAAGACGCTGGACATGATAAGCTGATCCAGACGGTGCGCGGCGCGGGGTATCGTTTTTCAACCAAAGCCTAAG
AGGGTGTAGAACAAATCAGT

Downstream 100 bases:

>100_bases
GCCGCATGCTAAGTCGCGTTGGTTGGTTGAGTTCATTGGTTTTGGTTTCATCCACCGCTTGGGCGTTGGATGAAGCGCTG
CCACACTATGAAAAAGTCAG

Product: phosphate regulon sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MVERLTWKKLAWELAFFYLPWVVVGWIFGSMPWLLLAATALQLFWHLNHQIRLSAWLWDEKRLTPPSGTGNWESLFNGIY
RLQQRHRKKRKELTNLIRRFRNGAESLPDAVVVFRSEGNIVWCNRLAQHLLGFRWPDDAGQPISNLLRTPDFIKYLKKDD
FTDPLEMRSPLNSDRMLELRIVPYTEGEHLMVVRDVTQLKQLEGMRRNFFANVSHELRTPMTVLQGYLEMTEDPDVLAGP
MWSKAHGVMTEQLNRMNGLVNQLLTLSKIEASPMVELDQVVDVPAMLEVLEKEAVSLSGEAQHKLHFEVDKSLKVLADED
QLRSAISNLVYNAVKYTPPGAQIDVRWYRTGKGACLEVEDKGEGIEPQHLHRLTERFYRVDKARSRDTGGSGLGLAIVKH
ALAHHDTHLDIYSKVGVGSKFSFVLPKRLVAKG

Sequences:

>Translated_433_residues
MVERLTWKKLAWELAFFYLPWVVVGWIFGSMPWLLLAATALQLFWHLNHQIRLSAWLWDEKRLTPPSGTGNWESLFNGIY
RLQQRHRKKRKELTNLIRRFRNGAESLPDAVVVFRSEGNIVWCNRLAQHLLGFRWPDDAGQPISNLLRTPDFIKYLKKDD
FTDPLEMRSPLNSDRMLELRIVPYTEGEHLMVVRDVTQLKQLEGMRRNFFANVSHELRTPMTVLQGYLEMTEDPDVLAGP
MWSKAHGVMTEQLNRMNGLVNQLLTLSKIEASPMVELDQVVDVPAMLEVLEKEAVSLSGEAQHKLHFEVDKSLKVLADED
QLRSAISNLVYNAVKYTPPGAQIDVRWYRTGKGACLEVEDKGEGIEPQHLHRLTERFYRVDKARSRDTGGSGLGLAIVKH
ALAHHDTHLDIYSKVGVGSKFSFVLPKRLVAKG
>Mature_433_residues
MVERLTWKKLAWELAFFYLPWVVVGWIFGSMPWLLLAATALQLFWHLNHQIRLSAWLWDEKRLTPPSGTGNWESLFNGIY
RLQQRHRKKRKELTNLIRRFRNGAESLPDAVVVFRSEGNIVWCNRLAQHLLGFRWPDDAGQPISNLLRTPDFIKYLKKDD
FTDPLEMRSPLNSDRMLELRIVPYTEGEHLMVVRDVTQLKQLEGMRRNFFANVSHELRTPMTVLQGYLEMTEDPDVLAGP
MWSKAHGVMTEQLNRMNGLVNQLLTLSKIEASPMVELDQVVDVPAMLEVLEKEAVSLSGEAQHKLHFEVDKSLKVLADED
QLRSAISNLVYNAVKYTPPGAQIDVRWYRTGKGACLEVEDKGEGIEPQHLHRLTERFYRVDKARSRDTGGSGLGLAIVKH
ALAHHDTHLDIYSKVGVGSKFSFVLPKRLVAKG

Specific function: Member of the two-component regulatory system phoR/phoB involved in the phosphate regulon genes expression. PhoR may function as a membrane-associated protein kinase that phosphorylates phoB in response to environmental signals [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=433, Percent_Identity=60.0461893764434, Blast_Score=501, Evalue=1e-143,
Organism=Escherichia coli, GI1788393, Length=240, Percent_Identity=30.8333333333333, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1786783, Length=263, Percent_Identity=31.5589353612167, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788549, Length=387, Percent_Identity=24.5478036175711, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788713, Length=407, Percent_Identity=23.5872235872236, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790346, Length=225, Percent_Identity=28.8888888888889, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1789149, Length=346, Percent_Identity=25.1445086705202, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI145693157, Length=220, Percent_Identity=27.2727272727273, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787894, Length=231, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI48994928, Length=276, Percent_Identity=29.7101449275362, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790436, Length=226, Percent_Identity=26.5486725663717, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789403, Length=272, Percent_Identity=29.0441176470588, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI87082128, Length=229, Percent_Identity=28.82096069869, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1789808, Length=238, Percent_Identity=29.8319327731092, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786912, Length=234, Percent_Identity=28.2051282051282, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790551, Length=220, Percent_Identity=31.3636363636364, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788279, Length=246, Percent_Identity=28.8617886178862, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790861, Length=215, Percent_Identity=30.6976744186047, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081816, Length=228, Percent_Identity=28.9473684210526, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1790300, Length=238, Percent_Identity=28.1512605042017, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR021766
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR014310 [H]

Pfam domain/function: PF11808 DUF3329; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49708; Mature: 49708

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVERLTWKKLAWELAFFYLPWVVVGWIFGSMPWLLLAATALQLFWHLNHQIRLSAWLWDE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECC
KRLTPPSGTGNWESLFNGIYRLQQRHRKKRKELTNLIRRFRNGAESLPDAVVVFRSEGNI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCEEEEEECCCCE
VWCNRLAQHLLGFRWPDDAGQPISNLLRTPDFIKYLKKDDFTDPLEMRSPLNSDRMLELR
EEHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEE
IVPYTEGEHLMVVRDVTQLKQLEGMRRNFFANVSHELRTPMTVLQGYLEMTEDPDVLAGP
EEEECCCCEEEEEHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHCCCCCCCEECC
MWSKAHGVMTEQLNRMNGLVNQLLTLSKIEASPMVELDQVVDVPAMLEVLEKEAVSLSGE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCHHHHHHHHHHHHHCCCCC
AQHKLHFEVDKSLKVLADEDQLRSAISNLVYNAVKYTPPGAQIDVRWYRTGKGACLEVED
CCCEEEEEECCCHHHCCCHHHHHHHHHHHHHHHHEECCCCCEEEEEEEECCCCCEEEECC
KGEGIEPQHLHRLTERFYRVDKARSRDTGGSGLGLAIVKHALAHHDTHLDIYSKVGVGSK
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCC
FSFVLPKRLVAKG
CHHHCCHHHHCCC
>Mature Secondary Structure
MVERLTWKKLAWELAFFYLPWVVVGWIFGSMPWLLLAATALQLFWHLNHQIRLSAWLWDE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECC
KRLTPPSGTGNWESLFNGIYRLQQRHRKKRKELTNLIRRFRNGAESLPDAVVVFRSEGNI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCEEEEEECCCCE
VWCNRLAQHLLGFRWPDDAGQPISNLLRTPDFIKYLKKDDFTDPLEMRSPLNSDRMLELR
EEHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEE
IVPYTEGEHLMVVRDVTQLKQLEGMRRNFFANVSHELRTPMTVLQGYLEMTEDPDVLAGP
EEEECCCCEEEEEHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHCCCCCCCEECC
MWSKAHGVMTEQLNRMNGLVNQLLTLSKIEASPMVELDQVVDVPAMLEVLEKEAVSLSGE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCHHHHHHHHHHHHHCCCCC
AQHKLHFEVDKSLKVLADEDQLRSAISNLVYNAVKYTPPGAQIDVRWYRTGKGACLEVED
CCCEEEEEECCCHHHCCCHHHHHHHHHHHHHHHHEECCCCCEEEEEEEECCCCCEEEECC
KGEGIEPQHLHRLTERFYRVDKARSRDTGGSGLGLAIVKHALAHHDTHLDIYSKVGVGSK
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCC
FSFVLPKRLVAKG
CHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2556368 [H]