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The map label for this gene is clpB-1

Identifier: 227080891

GI number: 227080891

Start: 717124

End: 719697

Strand: Direct

Name: clpB-1

Synonym: VCM66_0669

Alternate gene names: 227080891

Gene position: 717124-719697 (Clockwise)

Preceding gene: 227080890

Following gene: 227080892

Centisome position: 24.79

GC content: 49.61

Gene sequence:

>2574_bases
ATGCGTCTTGACAGATTTACGAGCAAATTTCAGATCGCGATTTCCGATGCCCAGTCATTAGCGCTCGGCCGTGATCATCA
ATATATCGAACCTGTGCACCTGATGGTGGCTCTGCTGGATCAAAACGGCAGCCCGATCCGCCCATTACTCACCATGCTTA
ATGTGGATGTGATGCAACTGCGCTCCAAACTTGGCGAAATGCTCGATCGTTTACCCAAAGTCAGTGGTATTGGTGGTGAT
GTACAGCTCTCTTCGGCGCTTGGCTCGCTGTTTAATCTGTGTGATAAGGTTGCGCAAAAGCGTCAAGATGCCTACATCTC
CTCTGAAATCTATCTACTTGCTGCGATTGAGGACAAAGGGCCACTGGGTCACTTACTCAAAGAGTTTGGACTGACCGAGA
AAAAAGTCTCTGAAGCAATAGAGAAGATTCGTGGTGGACAAAAGGTTAATGACCCGAATGCGGAAGAGCTGCGTCAAGCG
TTGGAAAAATTCACCATCGATCTGACTGAACGCGCTGAGCAGGGCAAACTCGATCCTGTGATCGGTCGTGATGATGAAAT
TCGCCGCACCATTCAAGTGCTGCAACGCCGTACCAAGAACAATCCGGTGATCATCGGTGAACCCGGTGTGGGTAAAACTG
CGATTGTGGAAGGCTTGGCGCAGCGCATCATTAATAATGAAGTACCAGAAGGCCTGCGTGGACGGCGAGTGTTGTCTTTA
GATATGGGCGCGCTAGTGGCAGGGGCGAAATACCGCGGCGAATTTGAAGAGCGCTTGAAATCAGTACTCAATGAGCTAGC
GAAAGAAGAAGGCAATATCATCCTCTTCATTGATGAGCTGCACACCATGGTTGGTGCGGGGAAAGGCGAAGGCTCGATGG
ATGCCGGCAACATGCTCAAACCCGCTTTGGCGCGTGGAGAATTGCACTGTGTCGGCGCAACCACTTTAGATGAGTACCGC
CAATACATCGAAAAAGATCCAGCGTTAGAGCGCCGCTTCCAAAAAGTGTTGGTGGATGAGCCCACGGTGGAAGATACCAT
CGCCATCTTGCGTGGATTGAAAGAGCGTTATGAACTGCATCACCATGTCGAAATCACAGATCCAGCGATTGTGGCGGCAG
CGAGCCTATCGCATCGCTATATTTCCGATCGCCAGTTGCCTGATAAAGCGATCGATTTGATCGATGAAGCGGCTTCAAGC
ATCCGGATGCAGATTGACTCCAAACCAGAAGCTCTGGATAAGCTTGAGCGCAAAATCATCCAACTGAAAATCGAGCAGCA
AGCGCTCTCTAATGAGCACGATGAAGCCAGTGAAAAACGCTTGGCGATCTTAAATGAAGAGCTGCAAGAGAAAGAGCGTG
ATTACGCGGAGTTGGAAGAAGTGTGGAAAGCTGAAAAGGCGGCACTCTCCGGCACGCAACATATTAAAGCGGCACTTGAA
CAAGCGCGGATGGATCTGGAAGTCGCTCGCCGTGCGGGGGATTTGAACCGCATGTCAGAGCTGCAATATGGTCGCATTCC
AGAGCTAGAAAAGCAGCTTGATCTGGCTGCGCAAGCCGAAATGCAGGAAATGACTCTGCTACGTAATAAAGTGACGGATG
CAGAAATTGCCGAAGTGCTTTCAAAACAGACTGGCATTCCAGTATCAAAAATGCTCGAAGCTGAGAAAGAAAAGTTGCTG
CGCATGGAAGACGTGCTGCATAAACGCGTGATTGGTCAAAAAGAAGCGGTGGAAGTGGTCGCGAATGCGATCCGCCGCAG
TCGCGCAGGGCTTTCTGATCCAAATCGTCCGATTGGCTCCTTCTTATTCTTGGGGCCAACAGGGGTCGGTAAAACCGAGC
TGTGTAAAACCTTGGCTAACTTCCTGTTTGACAGTGAAGATGCCATGGTACGTGTGGATATGTCGGAATTTATGGAGAAA
CACTCGGTTGCTCGTTTAGTCGGTGCGCCTCCAGGTTATGTCGGCTATGAAGAAGGTGGCTACTTAACGGAAGCTGTACG
CCGTAAACCTTACTCGGTGATTTTGCTCGATGAAGTGGAAAAGGCGCACCCAGATGTCTTCAATATTCTGCTGCAAGTCT
TGGATGATGGCCGCTTGACGGACGGACAAGGGCGCACGGTCGATTTTCGAAATACCGTTGTGATCATGACCTCCAACTTG
GGTTCAAGCCGTATCCAAGAAAATTTTGCAAGGTTGGATTATCAAGGTATCAAAGAACAAGTGATGGACGTAGTCAGCAA
ACACTTCCGTCCTGAGTTCTTGAACCGAGTGGATGAAAGTGTGGTATTCCACCCATTAGGACAAGAGCATATCAAGTCGA
TCGCTTCTATTCAGTTGGCCCGCCTACGTCAACGTTTGGCAGAGCGAGATTACCAACTAGAGGTTGATGATGAGGCGCTG
GATCTGATTGCTCATGTCGGCTTCGATCCGGTTTATGGTGCTCGTCCACTGAAACGCGCGATTCAACAAAATGTGGAAAA
CCCACTGGCCAAATCGATTCTGGCTGGTAAGTTCCTGCCTGGTTCACCTATTTTACTATCGGTAAAAGACGGGAATATTT
TCGCAAGCCAGTAG

Upstream 100 bases:

>100_bases
CAACAAGAAAGTTTCCTCAACTTCCCTTGAAAATCTGGTTCGTGATAACCATCTTTCCTAACGTATTCAATTTATCTCAG
ATGAGGTTAGGAAGGTTGGT

Downstream 100 bases:

>100_bases
CAGATACCTTATCTATCATAAGCAAAAGAGCCACTCAAAGTGGCTCTTTTGCTTGTTAATTGAACGATGAAGTGCGTTTT
TTAGTGGTTTTGCTGTTAAT

Product: clpB protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 857; Mature: 857

Protein sequence:

>857_residues
MRLDRFTSKFQIAISDAQSLALGRDHQYIEPVHLMVALLDQNGSPIRPLLTMLNVDVMQLRSKLGEMLDRLPKVSGIGGD
VQLSSALGSLFNLCDKVAQKRQDAYISSEIYLLAAIEDKGPLGHLLKEFGLTEKKVSEAIEKIRGGQKVNDPNAEELRQA
LEKFTIDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRTKNNPVIIGEPGVGKTAIVEGLAQRIINNEVPEGLRGRRVLSL
DMGALVAGAKYRGEFEERLKSVLNELAKEEGNIILFIDELHTMVGAGKGEGSMDAGNMLKPALARGELHCVGATTLDEYR
QYIEKDPALERRFQKVLVDEPTVEDTIAILRGLKERYELHHHVEITDPAIVAAASLSHRYISDRQLPDKAIDLIDEAASS
IRMQIDSKPEALDKLERKIIQLKIEQQALSNEHDEASEKRLAILNEELQEKERDYAELEEVWKAEKAALSGTQHIKAALE
QARMDLEVARRAGDLNRMSELQYGRIPELEKQLDLAAQAEMQEMTLLRNKVTDAEIAEVLSKQTGIPVSKMLEAEKEKLL
RMEDVLHKRVIGQKEAVEVVANAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKTLANFLFDSEDAMVRVDMSEFMEK
HSVARLVGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSSRIQENFARLDYQGIKEQVMDVVSKHFRPEFLNRVDESVVFHPLGQEHIKSIASIQLARLRQRLAERDYQLEVDDEAL
DLIAHVGFDPVYGARPLKRAIQQNVENPLAKSILAGKFLPGSPILLSVKDGNIFASQ

Sequences:

>Translated_857_residues
MRLDRFTSKFQIAISDAQSLALGRDHQYIEPVHLMVALLDQNGSPIRPLLTMLNVDVMQLRSKLGEMLDRLPKVSGIGGD
VQLSSALGSLFNLCDKVAQKRQDAYISSEIYLLAAIEDKGPLGHLLKEFGLTEKKVSEAIEKIRGGQKVNDPNAEELRQA
LEKFTIDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRTKNNPVIIGEPGVGKTAIVEGLAQRIINNEVPEGLRGRRVLSL
DMGALVAGAKYRGEFEERLKSVLNELAKEEGNIILFIDELHTMVGAGKGEGSMDAGNMLKPALARGELHCVGATTLDEYR
QYIEKDPALERRFQKVLVDEPTVEDTIAILRGLKERYELHHHVEITDPAIVAAASLSHRYISDRQLPDKAIDLIDEAASS
IRMQIDSKPEALDKLERKIIQLKIEQQALSNEHDEASEKRLAILNEELQEKERDYAELEEVWKAEKAALSGTQHIKAALE
QARMDLEVARRAGDLNRMSELQYGRIPELEKQLDLAAQAEMQEMTLLRNKVTDAEIAEVLSKQTGIPVSKMLEAEKEKLL
RMEDVLHKRVIGQKEAVEVVANAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKTLANFLFDSEDAMVRVDMSEFMEK
HSVARLVGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSSRIQENFARLDYQGIKEQVMDVVSKHFRPEFLNRVDESVVFHPLGQEHIKSIASIQLARLRQRLAERDYQLEVDDEAL
DLIAHVGFDPVYGARPLKRAIQQNVENPLAKSILAGKFLPGSPILLSVKDGNIFASQ
>Mature_857_residues
MRLDRFTSKFQIAISDAQSLALGRDHQYIEPVHLMVALLDQNGSPIRPLLTMLNVDVMQLRSKLGEMLDRLPKVSGIGGD
VQLSSALGSLFNLCDKVAQKRQDAYISSEIYLLAAIEDKGPLGHLLKEFGLTEKKVSEAIEKIRGGQKVNDPNAEELRQA
LEKFTIDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRTKNNPVIIGEPGVGKTAIVEGLAQRIINNEVPEGLRGRRVLSL
DMGALVAGAKYRGEFEERLKSVLNELAKEEGNIILFIDELHTMVGAGKGEGSMDAGNMLKPALARGELHCVGATTLDEYR
QYIEKDPALERRFQKVLVDEPTVEDTIAILRGLKERYELHHHVEITDPAIVAAASLSHRYISDRQLPDKAIDLIDEAASS
IRMQIDSKPEALDKLERKIIQLKIEQQALSNEHDEASEKRLAILNEELQEKERDYAELEEVWKAEKAALSGTQHIKAALE
QARMDLEVARRAGDLNRMSELQYGRIPELEKQLDLAAQAEMQEMTLLRNKVTDAEIAEVLSKQTGIPVSKMLEAEKEKLL
RMEDVLHKRVIGQKEAVEVVANAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKTLANFLFDSEDAMVRVDMSEFMEK
HSVARLVGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSSRIQENFARLDYQGIKEQVMDVVSKHFRPEFLNRVDESVVFHPLGQEHIKSIASIQLARLRQRLAERDYQLEVDDEAL
DLIAHVGFDPVYGARPLKRAIQQNVENPLAKSILAGKFLPGSPILLSVKDGNIFASQ

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family

Homologues:

Organism=Homo sapiens, GI13540606, Length=322, Percent_Identity=38.1987577639752, Blast_Score=211, Evalue=2e-54,
Organism=Escherichia coli, GI1788943, Length=857, Percent_Identity=76.3127187864644, Blast_Score=1347, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=333, Percent_Identity=41.4414414414414, Blast_Score=273, Evalue=3e-74,
Organism=Saccharomyces cerevisiae, GI6320464, Length=709, Percent_Identity=52.1861777150917, Blast_Score=754, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=863, Percent_Identity=41.5990730011588, Blast_Score=656, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CLPB_VIBCH (Q9KU18)

Other databases:

- EMBL:   AE003852
- PIR:   A82290
- RefSeq:   NP_230360.1
- ProteinModelPortal:   Q9KU18
- GeneID:   2615715
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0711
- TIGR:   VC_0711
- HOGENOM:   HBG413133
- OMA:   WHLLMSI
- ProtClustDB:   CLSK874082
- GO:   GO:0005737
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150
- Gene3D:   G3DSA:1.10.1780.10
- PRINTS:   PR00300
- SMART:   SM00382
- TIGRFAMs:   TIGR03346

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 95829; Mature: 95829

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00870 CLPAB_1; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDRFTSKFQIAISDAQSLALGRDHQYIEPVHLMVALLDQNGSPIRPLLTMLNVDVMQL
CCCHHHCCHHEEEEHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RSKLGEMLDRLPKVSGIGGDVQLSSALGSLFNLCDKVAQKRQDAYISSEIYLLAAIEDKG
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
PLGHLLKEFGLTEKKVSEAIEKIRGGQKVNDPNAEELRQALEKFTIDLTERAEQGKLDPV
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
IGRDDEIRRTIQVLQRRTKNNPVIIGEPGVGKTAIVEGLAQRIINNEVPEGLRGRRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DMGALVAGAKYRGEFEERLKSVLNELAKEEGNIILFIDELHTMVGAGKGEGSMDAGNMLK
CHHHHHHCHHHCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCHHH
PALARGELHCVGATTLDEYRQYIEKDPALERRFQKVLVDEPTVEDTIAILRGLKERYELH
HHHHCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HHVEITDPAIVAAASLSHRYISDRQLPDKAIDLIDEAASSIRMQIDSKPEALDKLERKII
HCEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHH
QLKIEQQALSNEHDEASEKRLAILNEELQEKERDYAELEEVWKAEKAALSGTQHIKAALE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QARMDLEVARRAGDLNRMSELQYGRIPELEKQLDLAAQAEMQEMTLLRNKVTDAEIAEVL
HHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SKQTGIPVSKMLEAEKEKLLRMEDVLHKRVIGQKEAVEVVANAIRRSRAGLSDPNRPIGS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKTLANFLFDSEDAMVRVDMSEFMEKHSVARLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
HHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSSRIQENFARLDYQGIKEQVMDVVSKHFRPEFLNRVDESVVFHPLGQEHIKSIASIQLA
CHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCHHHHHHHHHHHHH
RLRQRLAERDYQLEVDDEALDLIAHVGFDPVYGARPLKRAIQQNVENPLAKSILAGKFLP
HHHHHHHCCCCEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCC
GSPILLSVKDGNIFASQ
CCCEEEEEECCCEECCC
>Mature Secondary Structure
MRLDRFTSKFQIAISDAQSLALGRDHQYIEPVHLMVALLDQNGSPIRPLLTMLNVDVMQL
CCCHHHCCHHEEEEHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RSKLGEMLDRLPKVSGIGGDVQLSSALGSLFNLCDKVAQKRQDAYISSEIYLLAAIEDKG
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
PLGHLLKEFGLTEKKVSEAIEKIRGGQKVNDPNAEELRQALEKFTIDLTERAEQGKLDPV
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
IGRDDEIRRTIQVLQRRTKNNPVIIGEPGVGKTAIVEGLAQRIINNEVPEGLRGRRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DMGALVAGAKYRGEFEERLKSVLNELAKEEGNIILFIDELHTMVGAGKGEGSMDAGNMLK
CHHHHHHCHHHCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCHHH
PALARGELHCVGATTLDEYRQYIEKDPALERRFQKVLVDEPTVEDTIAILRGLKERYELH
HHHHCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HHVEITDPAIVAAASLSHRYISDRQLPDKAIDLIDEAASSIRMQIDSKPEALDKLERKII
HCEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHH
QLKIEQQALSNEHDEASEKRLAILNEELQEKERDYAELEEVWKAEKAALSGTQHIKAALE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QARMDLEVARRAGDLNRMSELQYGRIPELEKQLDLAAQAEMQEMTLLRNKVTDAEIAEVL
HHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SKQTGIPVSKMLEAEKEKLLRMEDVLHKRVIGQKEAVEVVANAIRRSRAGLSDPNRPIGS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKTLANFLFDSEDAMVRVDMSEFMEKHSVARLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
HHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSSRIQENFARLDYQGIKEQVMDVVSKHFRPEFLNRVDESVVFHPLGQEHIKSIASIQLA
CHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCHHHHHHHHHHHHH
RLRQRLAERDYQLEVDDEALDLIAHVGFDPVYGARPLKRAIQQNVENPLAKSILAGKFLP
HHHHHHHCCCCEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCC
GSPILLSVKDGNIFASQ
CCCEEEEEECCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301