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The map label for this gene is truB [H]

Identifier: 227080826

GI number: 227080826

Start: 645981

End: 646919

Strand: Direct

Name: truB [H]

Synonym: VCM66_0603

Alternate gene names: 227080826

Gene position: 645981-646919 (Clockwise)

Preceding gene: 227080825

Following gene: 227080827

Centisome position: 22.33

GC content: 51.86

Gene sequence:

>939_bases
ATGGCTCGTCGACGTAAAGGTCGGGTTATCCACGGTGTGATTCTGTTGGATAAGCCGACAGGTATCTCTTCCAACGACGC
TTTGCAAAAGGTTAAACGCCTCTATGGCGCTGAAAAAGCGGGACATACTGGCGCGCTTGATCCTCTGGCGACGGGCATGT
TGCCAATCTGCCTCGGTGAAGCCACTAAGTTTTCCCAATTTCTGCTCGATTCTGATAAACGTTATCGTGTGATCGCCAAA
TTGGGTGAACGTACCGACACCTCAGATTCTGATGGTCAAGTGGTACAAACGCGTCCTGTTCACGTGGATTACGATACGCT
GCTGGCCTGTATCGCCAAGTTTCGCGGTGAAACGGATCAAGTTCCATCCATGTTTTCAGCGTTGAAATACCAAGGTCGTC
CACTCTACGAATACGCCCGTCAGGGTATTGAAGTGCCGCGTGAAGCGCGCAAGATCACTGTGTATGAGATTGAGCTGCAT
CGCTTTGAAGGTGATGAAGTGGAGATGGAAGTACACTGCTCGAAAGGCACTTATATTCGTACCATAGTCGATGATCTGGG
TGAAATGCTGGGCTGTGGAGCGCATGTGACCATGCTGCGCCGTGTGGGTGTCGCTAACTATCCGTATGAGCGAATGGTGA
CGCTAGAGCAACTCAACGCTTTGGTTGAACAAGCCCATCGTGACGAAAAAGCGGTGGCGGACGTTCTCGATCCGCTATTA
CTGCCGATGGATACTGCCGTGGAAGCTTTACCCGAAGTCAACGTGATCCCAGAACTGATGACACTTATTCAGCATGGGCA
AGCAGTACAGGTTTCGGGGGCACCATCCGACGGTATGGTGCGCATTACTGGCGGCGAGCAGAAGCTATTCCTTGGCGTTG
GCGAGATTGACGATAACGGTAAGGTCGCGCCGAAACGCTTGGTGGTGTACGGCGAATAA

Upstream 100 bases:

>100_bases
CCTTGGTTGAAGGTATGCGTATGTCAAACCTCGTTACCGAAGTGATCAATAGTGACAAGCGCCGCAAGCATGAAGCGGGT
CGTGATGAGGACGAAGCGTA

Downstream 100 bases:

>100_bases
ATTCGTGGAAGGGCTCGCTGTATGGCGAGACATTCCGCTTGTGCGGATTAGGAATATTCCCTATAATCCGCGCTTCTCGT
GTCGGCTGAATCAGAGATTG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MARRRKGRVIHGVILLDKPTGISSNDALQKVKRLYGAEKAGHTGALDPLATGMLPICLGEATKFSQFLLDSDKRYRVIAK
LGERTDTSDSDGQVVQTRPVHVDYDTLLACIAKFRGETDQVPSMFSALKYQGRPLYEYARQGIEVPREARKITVYEIELH
RFEGDEVEMEVHCSKGTYIRTIVDDLGEMLGCGAHVTMLRRVGVANYPYERMVTLEQLNALVEQAHRDEKAVADVLDPLL
LPMDTAVEALPEVNVIPELMTLIQHGQAVQVSGAPSDGMVRITGGEQKLFLGVGEIDDNGKVAPKRLVVYGE

Sequences:

>Translated_312_residues
MARRRKGRVIHGVILLDKPTGISSNDALQKVKRLYGAEKAGHTGALDPLATGMLPICLGEATKFSQFLLDSDKRYRVIAK
LGERTDTSDSDGQVVQTRPVHVDYDTLLACIAKFRGETDQVPSMFSALKYQGRPLYEYARQGIEVPREARKITVYEIELH
RFEGDEVEMEVHCSKGTYIRTIVDDLGEMLGCGAHVTMLRRVGVANYPYERMVTLEQLNALVEQAHRDEKAVADVLDPLL
LPMDTAVEALPEVNVIPELMTLIQHGQAVQVSGAPSDGMVRITGGEQKLFLGVGEIDDNGKVAPKRLVVYGE
>Mature_311_residues
ARRRKGRVIHGVILLDKPTGISSNDALQKVKRLYGAEKAGHTGALDPLATGMLPICLGEATKFSQFLLDSDKRYRVIAKL
GERTDTSDSDGQVVQTRPVHVDYDTLLACIAKFRGETDQVPSMFSALKYQGRPLYEYARQGIEVPREARKITVYEIELHR
FEGDEVEMEVHCSKGTYIRTIVDDLGEMLGCGAHVTMLRRVGVANYPYERMVTLEQLNALVEQAHRDEKAVADVLDPLLL
PMDTAVEALPEVNVIPELMTLIQHGQAVQVSGAPSDGMVRITGGEQKLFLGVGEIDDNGKVAPKRLVVYGE

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=269, Percent_Identity=32.7137546468402, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI215599015, Length=252, Percent_Identity=29.7619047619048, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI4503337, Length=252, Percent_Identity=29.7619047619048, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI2367200, Length=310, Percent_Identity=66.4516129032258, Blast_Score=427, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17553978, Length=243, Percent_Identity=29.6296296296296, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324037, Length=191, Percent_Identity=30.3664921465969, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323204, Length=244, Percent_Identity=29.5081967213115, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI281364189, Length=236, Percent_Identity=31.7796610169492, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364187, Length=236, Percent_Identity=31.7796610169492, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364185, Length=236, Percent_Identity=31.7796610169492, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364183, Length=236, Percent_Identity=31.7796610169492, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI62471759, Length=236, Percent_Identity=31.7796610169492, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17975520, Length=236, Percent_Identity=31.7796610169492, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 34488; Mature: 34357

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRRKGRVIHGVILLDKPTGISSNDALQKVKRLYGAEKAGHTGALDPLATGMLPICLGE
CCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHCC
ATKFSQFLLDSDKRYRVIAKLGERTDTSDSDGQVVQTRPVHVDYDTLLACIAKFRGETDQ
HHHHHHHHHCCCCCEEHHHHHCCCCCCCCCCCCEEEECCCEECHHHHHHHHHHHCCCCCH
VPSMFSALKYQGRPLYEYARQGIEVPREARKITVYEIELHRFEGDEVEMEVHCSKGTYIR
HHHHHHHHHCCCCCHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCEEEEEEEECCCCCHH
TIVDDLGEMLGCGAHVTMLRRVGVANYPYERMVTLEQLNALVEQAHRDEKAVADVLDPLL
HHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
LPMDTAVEALPEVNVIPELMTLIQHGQAVQVSGAPSDGMVRITGGEQKLFLGVGEIDDNG
CCHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCEEEEEEECEECCCC
KVAPKRLVVYGE
CCCCEEEEEECC
>Mature Secondary Structure 
ARRRKGRVIHGVILLDKPTGISSNDALQKVKRLYGAEKAGHTGALDPLATGMLPICLGE
CCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHCC
ATKFSQFLLDSDKRYRVIAKLGERTDTSDSDGQVVQTRPVHVDYDTLLACIAKFRGETDQ
HHHHHHHHHCCCCCEEHHHHHCCCCCCCCCCCCEEEECCCEECHHHHHHHHHHHCCCCCH
VPSMFSALKYQGRPLYEYARQGIEVPREARKITVYEIELHRFEGDEVEMEVHCSKGTYIR
HHHHHHHHHCCCCCHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCEEEEEEEECCCCCHH
TIVDDLGEMLGCGAHVTMLRRVGVANYPYERMVTLEQLNALVEQAHRDEKAVADVLDPLL
HHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
LPMDTAVEALPEVNVIPELMTLIQHGQAVQVSGAPSDGMVRITGGEQKLFLGVGEIDDNG
CCHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCEEEEEEECEECCCC
KVAPKRLVVYGE
CCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA