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The map label for this gene is dacB [H]

Identifier: 227080813

GI number: 227080813

Start: 630734

End: 632197

Strand: Reverse

Name: dacB [H]

Synonym: VCM66_0590

Alternate gene names: 227080813

Gene position: 632197-630734 (Counterclockwise)

Preceding gene: 227080814

Following gene: 227080811

Centisome position: 21.86

GC content: 47.68

Gene sequence:

>1464_bases
TTGGTGGATGTTATTGCTATGCTTTTTCGCTTCATACCTGTTTGGTTACTCTCTATTAGCGGTTTTCTAATAGCCTCTCC
GATTTACGCACAAACACCATTGACTGCCGCAACGACTAAACTTCCTCAAGGGGCACGTTATAGCCTATTGATTGAAGATG
TCGCATTACAGCAGAACACTCTCGAACTCAATACTCATCTGTACTATCCCCCCGCTAGCACCCAGAAGATTTTGACGGCA
CTCGCCGCAAAATTAGAACTGGGTGATAAGTTTCGCTTTCACACTGATTTAATGCGTTCAGGGCAAGATTGGATCATTCG
CTTTTCAGGCGACCCCACCCTGACCACCGCAGATTTAACGACATTGCTCAAAGCGATGAAAGCGCAAAGTGGCGGTAGGA
TTGAGGGCGATTTGTGGCTGGATAATAGTGTATTTAGTGGATATGAGCGTGCGGTAGGCTGGCCATGGGATATTTTAGGC
GTCTGCTATAGCGCCCCAGCCAGTGCCATCAACCTCGATGCTAACTGTATCCAGGCGTCTATTTATACCGAACCACAAGG
TAAAACGCGCGTTTATGTACCAGAGCACTACCCTGTGCATGTTCAGTCGCAAGCCATCAGCGTGACACAGAGCGAACAAG
AGAGTTTACTGTGTGACTTAGAACTGACGGCAACGCCTGAGAATCACTATACGCTGGATGGCTGTTTAGCCCTACGAGAC
AAACCCTTACCACTAAAATTTGCGGTGCAAGATACTGGGATCTACACCCAGCGAGTGGTCTATCGTCTCCTCAGCCAGCT
AAACATCGAGCTCAAAGGGAAGATAAAAGTCGGTAAAGCAAATACCAAACAAGCGCAGAAAATCGCTTCTCATCACTCCC
AGCCGCTGCCTGTGTTACTGAAAACCATGTTGCAAGAGTCCGACAACCTGATCGCCGATACCTTGACCAAAGCCTTGGGA
CACCGTTTTTACTCTCAACCCGGTAGCTTTACCAACGGAACACAAGCCATTAAACAGATTTTTTACTCGCGCACGGGCAT
TTCATTAGAAGATACTCAGCTCGCCGATGGCTCTGGCCTTTCACGTAATAACCGGATGCGTCCACAAGTGATGCTGGAAA
CTCTTCGCTACCTTTATCAGCACGAAGCTGAGCTTGGTTTAATTGCTATGCTGCCTTCAGCGGGAGAATCGGGCACTTTG
CAATATCGACGCAGTATGCGTGCGCCGCAAATCAGTGGCCAAATTAAAGCGAAAAGTGGTTCACTTTATGGCACTTACAA
TATGGCGGGCTTTGTGATGGACGAAAATCAGCGCCCTAAGACTCTGTTTGTTCAATTCGTCACCGACTATTTCCCTCCGA
GATCCAATCCTGAGGTAGCGGTTGAGCCGCCGATTATCCAGTTTGAAACTCAGCTCTATCAAGAGCTTATTCAGTTTAAT
CGTTTGGCATCTAAGCCAAACTAG

Upstream 100 bases:

>100_bases
GGTCTACGTTACGACTTCTAATTGTTGACTTCAGGTCACACGCCAAACGCCTGCTCACTAGCAGGCGTTTTTCTATCTCG
TTATACTGCAGCAACGTTTC

Downstream 100 bases:

>100_bases
GCTACCTGAAAAATAAAAAGCGCCGTTTGGCGCTTTTTATTTATTTTTAGCTCAGGTTTGAAAACCATTACTTGATGGTC
ACTCGAGCGAACTTACGCTT

Product: D-alanyl-D-alanine carboxypeptidase/endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MVDVIAMLFRFIPVWLLSISGFLIASPIYAQTPLTAATTKLPQGARYSLLIEDVALQQNTLELNTHLYYPPASTQKILTA
LAAKLELGDKFRFHTDLMRSGQDWIIRFSGDPTLTTADLTTLLKAMKAQSGGRIEGDLWLDNSVFSGYERAVGWPWDILG
VCYSAPASAINLDANCIQASIYTEPQGKTRVYVPEHYPVHVQSQAISVTQSEQESLLCDLELTATPENHYTLDGCLALRD
KPLPLKFAVQDTGIYTQRVVYRLLSQLNIELKGKIKVGKANTKQAQKIASHHSQPLPVLLKTMLQESDNLIADTLTKALG
HRFYSQPGSFTNGTQAIKQIFYSRTGISLEDTQLADGSGLSRNNRMRPQVMLETLRYLYQHEAELGLIAMLPSAGESGTL
QYRRSMRAPQISGQIKAKSGSLYGTYNMAGFVMDENQRPKTLFVQFVTDYFPPRSNPEVAVEPPIIQFETQLYQELIQFN
RLASKPN

Sequences:

>Translated_487_residues
MVDVIAMLFRFIPVWLLSISGFLIASPIYAQTPLTAATTKLPQGARYSLLIEDVALQQNTLELNTHLYYPPASTQKILTA
LAAKLELGDKFRFHTDLMRSGQDWIIRFSGDPTLTTADLTTLLKAMKAQSGGRIEGDLWLDNSVFSGYERAVGWPWDILG
VCYSAPASAINLDANCIQASIYTEPQGKTRVYVPEHYPVHVQSQAISVTQSEQESLLCDLELTATPENHYTLDGCLALRD
KPLPLKFAVQDTGIYTQRVVYRLLSQLNIELKGKIKVGKANTKQAQKIASHHSQPLPVLLKTMLQESDNLIADTLTKALG
HRFYSQPGSFTNGTQAIKQIFYSRTGISLEDTQLADGSGLSRNNRMRPQVMLETLRYLYQHEAELGLIAMLPSAGESGTL
QYRRSMRAPQISGQIKAKSGSLYGTYNMAGFVMDENQRPKTLFVQFVTDYFPPRSNPEVAVEPPIIQFETQLYQELIQFN
RLASKPN
>Mature_487_residues
MVDVIAMLFRFIPVWLLSISGFLIASPIYAQTPLTAATTKLPQGARYSLLIEDVALQQNTLELNTHLYYPPASTQKILTA
LAAKLELGDKFRFHTDLMRSGQDWIIRFSGDPTLTTADLTTLLKAMKAQSGGRIEGDLWLDNSVFSGYERAVGWPWDILG
VCYSAPASAINLDANCIQASIYTEPQGKTRVYVPEHYPVHVQSQAISVTQSEQESLLCDLELTATPENHYTLDGCLALRD
KPLPLKFAVQDTGIYTQRVVYRLLSQLNIELKGKIKVGKANTKQAQKIASHHSQPLPVLLKTMLQESDNLIADTLTKALG
HRFYSQPGSFTNGTQAIKQIFYSRTGISLEDTQLADGSGLSRNNRMRPQVMLETLRYLYQHEAELGLIAMLPSAGESGTL
QYRRSMRAPQISGQIKAKSGSLYGTYNMAGFVMDENQRPKTLFVQFVTDYFPPRSNPEVAVEPPIIQFETQLYQELIQFN
RLASKPN

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=460, Percent_Identity=36.5217391304348, Blast_Score=324, Evalue=7e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 54376; Mature: 54376

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDVIAMLFRFIPVWLLSISGFLIASPIYAQTPLTAATTKLPQGARYSLLIEDVALQQNT
CHHHHHHHHHHHHHHHHHHCCHHHCCCCHHCCCCCHHHHCCCCCCCEEEEEEHHHHCCCE
LELNTHLYYPPASTQKILTALAAKLELGDKFRFHTDLMRSGQDWIIRFSGDPTLTTADLT
EEEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCCCCEEHHHHH
TLLKAMKAQSGGRIEGDLWLDNSVFSGYERAVGWPWDILGVCYSAPASAINLDANCIQAS
HHHHHHHHCCCCCEECEEEECCHHHCCHHHHCCCCHHHHHHHHCCCCCEEECCHHHEEEE
IYTEPQGKTRVYVPEHYPVHVQSQAISVTQSEQESLLCDLELTATPENHYTLDGCLALRD
EEECCCCCEEEECCCCCCEEEECCEEEEECCCCCCEEEEEEEEECCCCCEEEEEEEEECC
KPLPLKFAVQDTGIYTQRVVYRLLSQLNIELKGKIKVGKANTKQAQKIASHHSQPLPVLL
CCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEEEEEECCCCHHHHHHHHHHCCCCHHHHH
KTMLQESDNLIADTLTKALGHRFYSQPGSFTNGTQAIKQIFYSRTGISLEDTQLADGSGL
HHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
SRNNRMRPQVMLETLRYLYQHEAELGLIAMLPSAGESGTLQYRRSMRAPQISGQIKAKSG
CCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHCCCCCCCCEEEECCC
SLYGTYNMAGFVMDENQRPKTLFVQFVTDYFPPRSNPEVAVEPPIIQFETQLYQELIQFN
CEEEEECEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEECCCEEHHHHHHHHHHHHHH
RLASKPN
HHHCCCC
>Mature Secondary Structure
MVDVIAMLFRFIPVWLLSISGFLIASPIYAQTPLTAATTKLPQGARYSLLIEDVALQQNT
CHHHHHHHHHHHHHHHHHHCCHHHCCCCHHCCCCCHHHHCCCCCCCEEEEEEHHHHCCCE
LELNTHLYYPPASTQKILTALAAKLELGDKFRFHTDLMRSGQDWIIRFSGDPTLTTADLT
EEEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCCCCEEHHHHH
TLLKAMKAQSGGRIEGDLWLDNSVFSGYERAVGWPWDILGVCYSAPASAINLDANCIQAS
HHHHHHHHCCCCCEECEEEECCHHHCCHHHHCCCCHHHHHHHHCCCCCEEECCHHHEEEE
IYTEPQGKTRVYVPEHYPVHVQSQAISVTQSEQESLLCDLELTATPENHYTLDGCLALRD
EEECCCCCEEEECCCCCCEEEECCEEEEECCCCCCEEEEEEEEECCCCCEEEEEEEEECC
KPLPLKFAVQDTGIYTQRVVYRLLSQLNIELKGKIKVGKANTKQAQKIASHHSQPLPVLL
CCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEEEEEECCCCHHHHHHHHHHCCCCHHHHH
KTMLQESDNLIADTLTKALGHRFYSQPGSFTNGTQAIKQIFYSRTGISLEDTQLADGSGL
HHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
SRNNRMRPQVMLETLRYLYQHEAELGLIAMLPSAGESGTLQYRRSMRAPQISGQIKAKSG
CCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHCCCCCCCCEEEECCC
SLYGTYNMAGFVMDENQRPKTLFVQFVTDYFPPRSNPEVAVEPPIIQFETQLYQELIQFN
CEEEEECEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEECCCEEHHHHHHHHHHHHHH
RLASKPN
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]