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The map label for this gene is ispD

Identifier: 227080711

GI number: 227080711

Start: 517511

End: 518209

Strand: Direct

Name: ispD

Synonym: VCM66_0486

Alternate gene names: 227080711

Gene position: 517511-518209 (Clockwise)

Preceding gene: 227080710

Following gene: 227080712

Centisome position: 17.89

GC content: 52.5

Gene sequence:

>699_bases
ATGAACAACATGACAGCGATTGTCCCCGCCGCGGGTGTGGGTAGCCGCATGCAAGCGGATCGCCCTAAGCAGTATTTAAC
CTTGCTCGATAAAACGGTGCTCGAACACACCGTTGAACATCTTTTAGAACATCCTCTCATCGAACACGTGGTAGTGGCGG
TGAGTGCTGATGACCCCTATTTTGCGAATTTGCCTCTGGCGCATCATCCGCGTGTGATTCGTGTCGATGGCGGCAAAGAG
CGGGCGGATTCGGTATTGTCGGCACTGGAGTATGTCTGCCAGCATCGCCTCAGTGAATGGGTATTGGTGCATGATGCGGC
GCGTCCGTGTGTGACTCACGCCGATATCACTCAGCTCATCACCACGGCTTTAGCTCATCCGATTGGTGCGATTTTGGCCT
CGCCCGTGCGGGATACCATGAAACGTGGCGATCACTTACAGCAGATTGTGCATACGGTCGATCGCACTGCGCTCTGGCAT
GCGCTGACACCGCAAATGTTTCGTGCCCAATCACTGCGTGAGAGGTTATTCGCCGCTTTACAGCAGCAAGTCACGATTAC
CGATGAAGCCTCAGCCTTTGAATGGCGTGGTGAAAAGCCTGCTTTAGTGGCAGGACGTGCCGATAATCTCAAAATTACTC
AACCTGAAGATCTCGCGTTGGCCGAGTTCTATTTAAGTCGTAATAAGGAAAAATCATGA

Upstream 100 bases:

>100_bases
GCCAAGGGCTAGATGCGATTGAAGAGCGTGCCCGTAACGAATTGGGTATGGTCAAAGATGGAGAAACCTTCTATCGCATC
ATCGGTGAGGAGTCGCGCCA

Downstream 100 bases:

>100_bases
TCAGAATTGGCCACGGCTTTGATGTACACCGTTTTGGCGGCGAAGGTCCCATCATTATTGGTGGAGTGAAGATCCCTTAT
GAACAAGGATTGATCGCCCA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MNNMTAIVPAAGVGSRMQADRPKQYLTLLDKTVLEHTVEHLLEHPLIEHVVVAVSADDPYFANLPLAHHPRVIRVDGGKE
RADSVLSALEYVCQHRLSEWVLVHDAARPCVTHADITQLITTALAHPIGAILASPVRDTMKRGDHLQQIVHTVDRTALWH
ALTPQMFRAQSLRERLFAALQQQVTITDEASAFEWRGEKPALVAGRADNLKITQPEDLALAEFYLSRNKEKS

Sequences:

>Translated_232_residues
MNNMTAIVPAAGVGSRMQADRPKQYLTLLDKTVLEHTVEHLLEHPLIEHVVVAVSADDPYFANLPLAHHPRVIRVDGGKE
RADSVLSALEYVCQHRLSEWVLVHDAARPCVTHADITQLITTALAHPIGAILASPVRDTMKRGDHLQQIVHTVDRTALWH
ALTPQMFRAQSLRERLFAALQQQVTITDEASAFEWRGEKPALVAGRADNLKITQPEDLALAEFYLSRNKEKS
>Mature_232_residues
MNNMTAIVPAAGVGSRMQADRPKQYLTLLDKTVLEHTVEHLLEHPLIEHVVVAVSADDPYFANLPLAHHPRVIRVDGGKE
RADSVLSALEYVCQHRLSEWVLVHDAARPCVTHADITQLITTALAHPIGAILASPVRDTMKRGDHLQQIVHTVDRTALWH
ALTPQMFRAQSLRERLFAALQQQVTITDEASAFEWRGEKPALVAGRADNLKITQPEDLALAEFYLSRNKEKS

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=240, Percent_Identity=25.4166666666667, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1789104, Length=226, Percent_Identity=55.3097345132743, Blast_Score=247, Evalue=5e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_VIBCH (Q9KUJ2)

Other databases:

- EMBL:   AE003852
- PIR:   C82311
- RefSeq:   NP_230179.1
- ProteinModelPortal:   Q9KUJ2
- SMR:   Q9KUJ2
- GeneID:   2615819
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0528
- TIGR:   VC_0528
- HOGENOM:   HBG672839
- OMA:   FPQGAIL
- ProtClustDB:   PRK00155
- BRENDA:   2.7.7.60
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25902; Mature: 25902

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS01295 ISPD; PS00485 A_DEAMINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNMTAIVPAAGVGSRMQADRPKQYLTLLDKTVLEHTVEHLLEHPLIEHVVVAVSADDPY
CCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHEEEEECCCCC
FANLPLAHHPRVIRVDGGKERADSVLSALEYVCQHRLSEWVLVHDAARPCVTHADITQLI
EECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHEEEHCCCCCCHHHHHHHHHHH
TTALAHPIGAILASPVRDTMKRGDHLQQIVHTVDRTALWHALTPQMFRAQSLRERLFAAL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
QQQVTITDEASAFEWRGEKPALVAGRADNLKITQPEDLALAEFYLSRNKEKS
HHHCCCCCCCCHHHCCCCCCCEEECCCCCCEECCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNNMTAIVPAAGVGSRMQADRPKQYLTLLDKTVLEHTVEHLLEHPLIEHVVVAVSADDPY
CCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHEEEEECCCCC
FANLPLAHHPRVIRVDGGKERADSVLSALEYVCQHRLSEWVLVHDAARPCVTHADITQLI
EECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHEEEHCCCCCCHHHHHHHHHHH
TTALAHPIGAILASPVRDTMKRGDHLQQIVHTVDRTALWHALTPQMFRAQSLRERLFAAL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
QQQVTITDEASAFEWRGEKPALVAGRADNLKITQPEDLALAEFYLSRNKEKS
HHHCCCCCCCCHHHCCCCCCCEEECCCCCCEECCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301