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The map label for this gene is obgE [H]

Identifier: 227080648

GI number: 227080648

Start: 451969

End: 453156

Strand: Direct

Name: obgE [H]

Synonym: VCM66_0422

Alternate gene names: 227080648

Gene position: 451969-453156 (Clockwise)

Preceding gene: 227080647

Following gene: 227080649

Centisome position: 15.63

GC content: 48.48

Gene sequence:

>1188_bases
TTGAGTGGAGGAAAAATGAAATTCGTAGATGAAGCGGTAATTAAAGTTCAAGCTGGTGATGGCGGCAACGGTGTTGTGAG
CTTCTGGCGTGAAAAATTCGTTACCAATGGCGGCCCTGATGGTGGTGATGGTGGTGATGGCGGCGATGTGTATATGGTCG
CTGATGAAAACCTCAACACTTTGATTGACTATCGTTTCCAGCGCTTTTATGAAGCGGAGCGTGGTAAAAACGGCGGTGGC
GGTAACTGTACAGGTAAAAGCGGTAAAGACAAAGAGCTGCGCGTACCGGTAGGTACTCGTGCCGTCGATATTCACACCAA
TGAAATCATTGGTGAAGTGGCCGAACATGGCAAAAAAGTCATGATCGCCAAAGGTGGTTGGCACGGTTTGGGCAACGCCC
GTTTTAAATCCTCGGTGAACCGCTCTCCACGTCAAAAAACCTTGGGTACCAAGGGTGAGCTGCGTGATATTCGTTTAGAG
CTGCTGCTGTTGGCTGACGTTGGTATGCTCGGCATGCCAAATGCGGGTAAATCGACCTTCATTCGTGCTGTTTCTGCGGC
TAAACCTAAAGTGGCGGATTACCCGTTTACCACGCTAGTACCAAGCCTTGGTGTGGTGAGCGTGCTGCCGGAGAAAAGCT
TTGTGGTGGCTGACATCCCAGGCTTGATTGAAGGCGCTGCGGAAGGGGCTGGTTTAGGGATTCGTTTCCTTAAGCACCTT
GAACGTTGTCGCGTGCTGCTACACATGATTGATATCATGCCTGCTGATCAAAGCGATCCTGCACACAATGCGTTGACCAT
TATTGATGAGCTTGAACAGTACAGTGAAAAGCTGGCGAAAAAGCCACGTTGGTTAGTGTTCAACAAAGTTGATCTGATGT
CAGAAGAAGAAGCGGATGAGATCATTCAAAACATCATCGATGCTCTGGCATGGGAAGGCGATTACTTCAAAATCTCTGCG
GCGAATCGTCAAGGCACCAAAGAGCTGTGCATGAAGCTTGCCGAGTTCATGGATACCTTACCAAGAGAAGCGGAAGAGAA
AACAGAAGCCGAAAAAGTCGATTTCACATGGGATTATAACCATAAAGACGGATTGGCTGGTCGTGAAGTGATCACTGAAG
ATGATGACGATTGGGATGATTGGGACGATGAAGAAGATGACGGCCACGTTATCTATGTTCGTGACTGA

Upstream 100 bases:

>100_bases
CCTAGACCCTAGACCTGAATGTAGCCTGATCCAAGCAGGCGATTGTTTTCTGGGATCTACGTTCTCAGGTTTACTAGACT
GATTAAGATTTCCCACGAGA

Downstream 100 bases:

>100_bases
TTAACTTACTGGTATGCCGCAACTTAGTTGCGGCTTTTTTGTGTCTAAATCAACTTTCCTAAAGTTTGTTTCGGGCACAC
TTACTCTCTCTTCGGCATAT

Product: GTPase ObgE

Products: NA

Alternate protein names: CgtA; GTP-binding protein Obg [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MSGGKMKFVDEAVIKVQAGDGGNGVVSFWREKFVTNGGPDGGDGGDGGDVYMVADENLNTLIDYRFQRFYEAERGKNGGG
GNCTGKSGKDKELRVPVGTRAVDIHTNEIIGEVAEHGKKVMIAKGGWHGLGNARFKSSVNRSPRQKTLGTKGELRDIRLE
LLLLADVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVSVLPEKSFVVADIPGLIEGAAEGAGLGIRFLKHL
ERCRVLLHMIDIMPADQSDPAHNALTIIDELEQYSEKLAKKPRWLVFNKVDLMSEEEADEIIQNIIDALAWEGDYFKISA
ANRQGTKELCMKLAEFMDTLPREAEEKTEAEKVDFTWDYNHKDGLAGREVITEDDDDWDDWDDEEDDGHVIYVRD

Sequences:

>Translated_395_residues
MSGGKMKFVDEAVIKVQAGDGGNGVVSFWREKFVTNGGPDGGDGGDGGDVYMVADENLNTLIDYRFQRFYEAERGKNGGG
GNCTGKSGKDKELRVPVGTRAVDIHTNEIIGEVAEHGKKVMIAKGGWHGLGNARFKSSVNRSPRQKTLGTKGELRDIRLE
LLLLADVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVSVLPEKSFVVADIPGLIEGAAEGAGLGIRFLKHL
ERCRVLLHMIDIMPADQSDPAHNALTIIDELEQYSEKLAKKPRWLVFNKVDLMSEEEADEIIQNIIDALAWEGDYFKISA
ANRQGTKELCMKLAEFMDTLPREAEEKTEAEKVDFTWDYNHKDGLAGREVITEDDDDWDDWDDEEDDGHVIYVRD
>Mature_394_residues
SGGKMKFVDEAVIKVQAGDGGNGVVSFWREKFVTNGGPDGGDGGDGGDVYMVADENLNTLIDYRFQRFYEAERGKNGGGG
NCTGKSGKDKELRVPVGTRAVDIHTNEIIGEVAEHGKKVMIAKGGWHGLGNARFKSSVNRSPRQKTLGTKGELRDIRLEL
LLLADVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVSVLPEKSFVVADIPGLIEGAAEGAGLGIRFLKHLE
RCRVLLHMIDIMPADQSDPAHNALTIIDELEQYSEKLAKKPRWLVFNKVDLMSEEEADEIIQNIIDALAWEGDYFKISAA
NRQGTKELCMKLAEFMDTLPREAEEKTEAEKVDFTWDYNHKDGLAGREVITEDDDDWDDWDDEEDDGHVIYVRD

Specific function: An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. It may play a role in control of the cell cycle, stress response, ribosome biogenesis and in t

COG id: COG0536

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI24308117, Length=334, Percent_Identity=38.9221556886228, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI111955139, Length=323, Percent_Identity=35.6037151702786, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI111955063, Length=157, Percent_Identity=40.1273885350318, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1789574, Length=361, Percent_Identity=68.1440443213296, Blast_Score=450, Evalue=1e-128,
Organism=Escherichia coli, GI1787454, Length=129, Percent_Identity=29.4573643410853, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17508313, Length=342, Percent_Identity=37.4269005847953, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17552324, Length=313, Percent_Identity=32.9073482428115, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17509631, Length=131, Percent_Identity=32.0610687022901, Blast_Score=71, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6321962, Length=166, Percent_Identity=37.9518072289157, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6319281, Length=126, Percent_Identity=32.5396825396825, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24585318, Length=307, Percent_Identity=35.8306188925081, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI20129375, Length=303, Percent_Identity=37.953795379538, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24640873, Length=132, Percent_Identity=31.0606060606061, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24640877, Length=132, Percent_Identity=31.0606060606061, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24640875, Length=132, Percent_Identity=31.0606060606061, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014100
- InterPro:   IPR006074
- InterPro:   IPR006073
- InterPro:   IPR006169
- InterPro:   IPR002917 [H]

Pfam domain/function: PF01018 GTP1_OBG; PF01926 MMR_HSR1 [H]

EC number: NA

Molecular weight: Translated: 43507; Mature: 43375

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00905 GTP1_OBG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGGKMKFVDEAVIKVQAGDGGNGVVSFWREKFVTNGGPDGGDGGDGGDVYMVADENLNT
CCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHH
LIDYRFQRFYEAERGKNGGGGNCTGKSGKDKELRVPVGTRAVDIHTNEIIGEVAEHGKKV
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCEEEEECHHHHHHHHHHCCCEE
MIAKGGWHGLGNARFKSSVNRSPRQKTLGTKGELRDIRLELLLLADVGMLGMPNAGKSTF
EEECCCCCCCCCHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHEECCCCCCCCCCCCHHHH
IRAVSAAKPKVADYPFTTLVPSLGVVSVLPEKSFVVADIPGLIEGAAEGAGLGIRFLKHL
HHHHHHCCCCCCCCCHHHHHCCCCCEEECCCCCEEEECCCHHHHHCCCCCCHHHHHHHHH
ERCRVLLHMIDIMPADQSDPAHNALTIIDELEQYSEKLAKKPRWLVFNKVDLMSEEEADE
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHCCCHHHHH
IIQNIIDALAWEGDYFKISAANRQGTKELCMKLAEFMDTLPREAEEKTEAEKVDFTWDYN
HHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEEECCC
HKDGLAGREVITEDDDDWDDWDDEEDDGHVIYVRD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure 
SGGKMKFVDEAVIKVQAGDGGNGVVSFWREKFVTNGGPDGGDGGDGGDVYMVADENLNT
CCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHH
LIDYRFQRFYEAERGKNGGGGNCTGKSGKDKELRVPVGTRAVDIHTNEIIGEVAEHGKKV
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCEEEEECHHHHHHHHHHCCCEE
MIAKGGWHGLGNARFKSSVNRSPRQKTLGTKGELRDIRLELLLLADVGMLGMPNAGKSTF
EEECCCCCCCCCHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHEECCCCCCCCCCCCHHHH
IRAVSAAKPKVADYPFTTLVPSLGVVSVLPEKSFVVADIPGLIEGAAEGAGLGIRFLKHL
HHHHHHCCCCCCCCCHHHHHCCCCCEEECCCCCEEEECCCHHHHHCCCCCCHHHHHHHHH
ERCRVLLHMIDIMPADQSDPAHNALTIIDELEQYSEKLAKKPRWLVFNKVDLMSEEEADE
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHCCCHHHHH
IIQNIIDALAWEGDYFKISAANRQGTKELCMKLAEFMDTLPREAEEKTEAEKVDFTWDYN
HHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEEECCC
HKDGLAGREVITEDDDDWDDWDDEEDDGHVIYVRD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]