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The map label for this gene is priB

Identifier: 227080579

GI number: 227080579

Start: 369296

End: 369598

Strand: Direct

Name: priB

Synonym: VCM66_0351

Alternate gene names: 227080579

Gene position: 369296-369598 (Clockwise)

Preceding gene: 227080578

Following gene: 227080580

Centisome position: 12.77

GC content: 45.54

Gene sequence:

>303_bases
ATGACCAATCGAATCGAGTTGAGCGGCGTTGTCGCAAAGGCTCTGGTTCGAAGCCAAAGTCCATCGGGTGTTAAACATTG
CCATTTTTGGCTAGAGCATCGCTCTACGATGGTAGAAGCTGACCTCCCAAGACAAGTTTTTTGTCGATTACCTGTGGTGG
TGAGTGGGGCTAGGTCAGAAGCGTTAACTCAAAACTTAGTACAAGGCAGTAGCATTTTGGTAAGTGGTTTTTTGGCTTAC
CAAACCAGCCGAAATGGCGTAGGTAAATTAGTGCTGCATGCCGACAAAATTTCTCAAATATAG

Upstream 100 bases:

>100_bases
AAAGCTGCTATTACTGAACCTTCAATCATGCTGAAAGCTCGTGAAGAGCGTGTAAAGCGTGACGAGATGAAGTTCGACGC
AGACGTTGAGTAATGGTTTG

Downstream 100 bases:

>100_bases
ATCAGGAGATAGCCCATGGCTCGTTTCTTCCGTCGTCGTAAATTCTGCCGTTTTACTGCAGAAGGCGTACAAGAGATTGA
TTACAAAGACGTAGCAACTC

Product: primosomal replication protein N

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 100; Mature: 99

Protein sequence:

>100_residues
MTNRIELSGVVAKALVRSQSPSGVKHCHFWLEHRSTMVEADLPRQVFCRLPVVVSGARSEALTQNLVQGSSILVSGFLAY
QTSRNGVGKLVLHADKISQI

Sequences:

>Translated_100_residues
MTNRIELSGVVAKALVRSQSPSGVKHCHFWLEHRSTMVEADLPRQVFCRLPVVVSGARSEALTQNLVQGSSILVSGFLAY
QTSRNGVGKLVLHADKISQI
>Mature_99_residues
TNRIELSGVVAKALVRSQSPSGVKHCHFWLEHRSTMVEADLPRQVFCRLPVVVSGARSEALTQNLVQGSSILVSGFLAYQ
TSRNGVGKLVLHADKISQI

Specific function: Binds single-stranded DNA at the primosome assembly site (PAS)

COG id: COG2965

COG function: function code L; Primosomal replication protein N

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SSB domain

Homologues:

Organism=Escherichia coli, GI1790645, Length=100, Percent_Identity=49, Blast_Score=105, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIB_VIBCH (Q9KUZ1)

Other databases:

- EMBL:   AE003852
- PIR:   A82333
- RefSeq:   NP_230021.1
- ProteinModelPortal:   Q9KUZ1
- SMR:   Q9KUZ1
- GeneID:   2615046
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0367
- TIGR:   VC_0367
- HOGENOM:   HBG632331
- OMA:   WCRMPVI
- ProtClustDB:   PRK02801
- HAMAP:   MF_00720
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000424
- Gene3D:   G3DSA:2.40.50.140

Pfam domain/function: PF00436 SSB; SSF50249 Nucleic_acid_OB

EC number: NA

Molecular weight: Translated: 10914; Mature: 10783

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: PS50935 SSB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRIELSGVVAKALVRSQSPSGVKHCHFWLEHRSTMVEADLPRQVFCRLPVVVSGARSE
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEECCCCCHHHHHCCCCEEECCCHH
ALTQNLVQGSSILVSGFLAYQTSRNGVGKLVLHADKISQI
HHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
TNRIELSGVVAKALVRSQSPSGVKHCHFWLEHRSTMVEADLPRQVFCRLPVVVSGARSE
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEECCCCCHHHHHCCCCEEECCCHH
ALTQNLVQGSSILVSGFLAYQTSRNGVGKLVLHADKISQI
HHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301