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The map label for this gene is bfr [H]

Identifier: 227080577

GI number: 227080577

Start: 368232

End: 368708

Strand: Direct

Name: bfr [H]

Synonym: VCM66_0349

Alternate gene names: 227080577

Gene position: 368232-368708 (Clockwise)

Preceding gene: 227080576

Following gene: 227080578

Centisome position: 12.73

GC content: 40.25

Gene sequence:

>477_bases
ATGAAAGGTGATCCAGTCATAATTCAACATCTCAATAAAGTACTGGCTAATGAGCTTATCGCCATCAATCAATACTTCTT
ACATGCACGCATGTATAAAGATTGGGGCTTGAAGCATCTTGCCGATAAGGAATACCACGAATCTATTGATGAGATGAAAC
ACGCTGACCATATTGTGGAAAGAATCTTGTTTTTAGAGGGGATTCCCAATCTACAAGATCTTGGCAAACTGATGATTGGT
GAAGATGTGCAAGAGATGTTGGAGTGCGATCTCAAACTTGAGTTGATTGCGATTCCTGATCTCAAAGAGGCGATCGCTTA
CGCCGAAAAAGTACGTGACTACGTTTCTCGTGATCTGTTCCAAGAGATCTTAGAAGATGAAGAAGAGCATGTTGATTGGT
TAGAAACTCAACTTGGTCTGATCGATATGACGGGTATCCAAAATTATCTGCAAGCTCAGTTTGTTGATGACGAATAA

Upstream 100 bases:

>100_bases
GCTGATTGCTCAGTTTAAACTGGCAAGTTAAGCACAGCTTCTTTTTGACTCTCCTCTAATTGGTTCTACAGTTAATAGAG
CCAAAGAGGAGGGCTATGCT

Downstream 100 bases:

>100_bases
TCCCCTGTACTAACACATATCATTCCTAGGCAGAAAACGCGTTTTTCTGCCTAACCGCTTCCTGTTTCTCCCTCGATAAT
TTCCTTGCTAAAGCTGCTTC

Product: bacterioferritin

Products: NA

Alternate protein names: BFR; Cytochrome b-557.5 [H]

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MKGDPVIIQHLNKVLANELIAINQYFLHARMYKDWGLKHLADKEYHESIDEMKHADHIVERILFLEGIPNLQDLGKLMIG
EDVQEMLECDLKLELIAIPDLKEAIAYAEKVRDYVSRDLFQEILEDEEEHVDWLETQLGLIDMTGIQNYLQAQFVDDE

Sequences:

>Translated_158_residues
MKGDPVIIQHLNKVLANELIAINQYFLHARMYKDWGLKHLADKEYHESIDEMKHADHIVERILFLEGIPNLQDLGKLMIG
EDVQEMLECDLKLELIAIPDLKEAIAYAEKVRDYVSRDLFQEILEDEEEHVDWLETQLGLIDMTGIQNYLQAQFVDDE
>Mature_158_residues
MKGDPVIIQHLNKVLANELIAINQYFLHARMYKDWGLKHLADKEYHESIDEMKHADHIVERILFLEGIPNLQDLGKLMIG
EDVQEMLECDLKLELIAIPDLKEAIAYAEKVRDYVSRDLFQEILEDEEEHVDWLETQLGLIDMTGIQNYLQAQFVDDE

Specific function: May perform analogous functions in iron detoxification and storage to that of animal ferritins [H]

COG id: COG2193

COG function: function code P; Bacterioferritin (cytochrome b1)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ferritin-like diiron domain [H]

Homologues:

Organism=Escherichia coli, GI1789733, Length=154, Percent_Identity=70.7792207792208, Blast_Score=223, Evalue=6e-60,

Paralogues:

None

Copy number: 1639 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002024
- InterPro:   IPR009040
- InterPro:   IPR012347
- InterPro:   IPR009078
- InterPro:   IPR008331 [H]

Pfam domain/function: PF00210 Ferritin [H]

EC number: NA

Molecular weight: Translated: 18491; Mature: 18491

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: PS50905 FERRITIN_LIKE ; PS00549 BACTERIOFERRITIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGDPVIIQHLNKVLANELIAINQYFLHARMYKDWGLKHLADKEYHESIDEMKHADHIVE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHH
RILFLEGIPNLQDLGKLMIGEDVQEMLECDLKLELIAIPDLKEAIAYAEKVRDYVSRDLF
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
QEILEDEEEHVDWLETQLGLIDMTGIQNYLQAQFVDDE
HHHHHCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKGDPVIIQHLNKVLANELIAINQYFLHARMYKDWGLKHLADKEYHESIDEMKHADHIVE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHH
RILFLEGIPNLQDLGKLMIGEDVQEMLECDLKLELIAIPDLKEAIAYAEKVRDYVSRDLF
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
QEILEDEEEHVDWLETQLGLIDMTGIQNYLQAQFVDDE
HHHHHCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1549605; 1904771 [H]