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The map label for this gene is dinF [H]

Identifier: 227080326

GI number: 227080326

Start: 87646

End: 89010

Strand: Reverse

Name: dinF [H]

Synonym: VCM66_0090

Alternate gene names: 227080326

Gene position: 89010-87646 (Counterclockwise)

Preceding gene: 227080327

Following gene: 227080317

Centisome position: 3.08

GC content: 51.36

Gene sequence:

>1365_bases
GTGTTCTGTGTGTCACTGATTTTGCAAACGTTGAAACAACCGAATGTGCATCGCCAAGTGCTGGCGATCGCGCTGCCTAT
GGTCCTGTCTAACATTACCGTTCCACTGCTCGGCTTGGTCGATGCTGCGGTGATCGGTCATCTCGAACACGCTTGGTATT
TAGGCGGCGTGGCACTCGGCAGCACTATGATTAGCGTGACATTCTGGCTGCTCGGTTTTTTACGCATGTCGACCACAGGC
TTAACCGCCCAAGCGCACGGTGCGCAAAATTCACAGCAGCTTGCTCGAGTTTTACTGCAAGGCAGCGTCATTGCACTGGG
TTTAGCCGCACTGTTTTTACTGTTTCACCGCCCTGTCGCCGAACTGATTTTTCACTTCAGCGATGCCAGCAGCGAAGTCA
AAACCTACGCTGAAGCCTATTTCTACATTCGTGCTTGGAGTGCCCCAGCCGCCTTGCTTAACTTTGTACTGCTTGGCTGG
TTACTCGGCACCCAAAATGCCCGCGCTCCGATGTGGATGGTGATCATCACCAACCTCACCAATATTGTGCTCGATCTGCT
GTTGGTACTCGGGCTTGGGCTGAAAGTCGAAGGAGCCGCCATTGCGTCGGTTATCGCCGATTATGCTGGTCTCTTGTTTG
GTCTCCTGTGCGTGGGGCGTTACTGGCGGCAGCATCAGCTCCCAGCCCCTTTCTCTTTCATCCCCTCTCTCACCAAAGAG
CTTTCCCGTTTAGTGGCGCTCAATCGTGACATCTTCCTACGCTCACTCTGTCTGCAAGCCGTATTTAGCTTTATGACCTT
TCAAGGCGCAGCGTTGGGAGATGACATCGTCGCTGCTAATGCGGTGTTGATGAGCTTTTTGATGATGATTTCCTACGGTA
TGGATGGATTTGCCTACGCGATGGAAGCCATGGTCGGTAAAGCAATTGGTGCCAAGGATGATCGCCAACTGCGTACTGCC
ATGATTAGCAGTACCTTTTGGGCTACCATGATCTGTTTGCTGTTAAGCCTGATCTTCTTAGGTTTTGGATCGGATCTGAT
CCAAATGATCACTAACATTCCGAGCGTTCAAGCAACGGCGGAACATTATCTGCCTTGGCTGGTCGCCATGCCATTAATCG
CCGTGTGGTGTTTTCTATTGGATGGTGTGTTTATCGGCGCGACCAAAGGCAAAGAGATGCGAAATAGCATGGCTGTTTCG
GCCGTAGCGTTCTTTGCCATTTACGGGTTCATGACACACTACGGTAATCACGCTCTGTGGCTAGCCATGCTCAGCTTTAT
GGCACTGCGTGGTTTTACCTTAGGGGCTACCTTTTATACTCAATGGCGCGCGGGGACTTTTTTACCGGCTCGCTTTTCCA
TTTAA

Upstream 100 bases:

>100_bases
ACCCCTGTTGTTGTTAACCTAGATTGGTAACCCGCCATCTCGGTCATGTCATAGCCCAACTATCCCCACTCAGTTACACT
AACCGCAAGTCGTTACTGAG

Downstream 100 bases:

>100_bases
ACCACCTCCGATCGTTTAAGTAGCAGCTGTTTAGATATCGCCATCCCGAAACATAGCAAAGCCCGCTGGCAGGTAAAACA
CCGAGCCAGCAGGCGCTTAC

Product: DNA-damage-inducible protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MFCVSLILQTLKQPNVHRQVLAIALPMVLSNITVPLLGLVDAAVIGHLEHAWYLGGVALGSTMISVTFWLLGFLRMSTTG
LTAQAHGAQNSQQLARVLLQGSVIALGLAALFLLFHRPVAELIFHFSDASSEVKTYAEAYFYIRAWSAPAALLNFVLLGW
LLGTQNARAPMWMVIITNLTNIVLDLLLVLGLGLKVEGAAIASVIADYAGLLFGLLCVGRYWRQHQLPAPFSFIPSLTKE
LSRLVALNRDIFLRSLCLQAVFSFMTFQGAALGDDIVAANAVLMSFLMMISYGMDGFAYAMEAMVGKAIGAKDDRQLRTA
MISSTFWATMICLLLSLIFLGFGSDLIQMITNIPSVQATAEHYLPWLVAMPLIAVWCFLLDGVFIGATKGKEMRNSMAVS
AVAFFAIYGFMTHYGNHALWLAMLSFMALRGFTLGATFYTQWRAGTFLPARFSI

Sequences:

>Translated_454_residues
MFCVSLILQTLKQPNVHRQVLAIALPMVLSNITVPLLGLVDAAVIGHLEHAWYLGGVALGSTMISVTFWLLGFLRMSTTG
LTAQAHGAQNSQQLARVLLQGSVIALGLAALFLLFHRPVAELIFHFSDASSEVKTYAEAYFYIRAWSAPAALLNFVLLGW
LLGTQNARAPMWMVIITNLTNIVLDLLLVLGLGLKVEGAAIASVIADYAGLLFGLLCVGRYWRQHQLPAPFSFIPSLTKE
LSRLVALNRDIFLRSLCLQAVFSFMTFQGAALGDDIVAANAVLMSFLMMISYGMDGFAYAMEAMVGKAIGAKDDRQLRTA
MISSTFWATMICLLLSLIFLGFGSDLIQMITNIPSVQATAEHYLPWLVAMPLIAVWCFLLDGVFIGATKGKEMRNSMAVS
AVAFFAIYGFMTHYGNHALWLAMLSFMALRGFTLGATFYTQWRAGTFLPARFSI
>Mature_454_residues
MFCVSLILQTLKQPNVHRQVLAIALPMVLSNITVPLLGLVDAAVIGHLEHAWYLGGVALGSTMISVTFWLLGFLRMSTTG
LTAQAHGAQNSQQLARVLLQGSVIALGLAALFLLFHRPVAELIFHFSDASSEVKTYAEAYFYIRAWSAPAALLNFVLLGW
LLGTQNARAPMWMVIITNLTNIVLDLLLVLGLGLKVEGAAIASVIADYAGLLFGLLCVGRYWRQHQLPAPFSFIPSLTKE
LSRLVALNRDIFLRSLCLQAVFSFMTFQGAALGDDIVAANAVLMSFLMMISYGMDGFAYAMEAMVGKAIGAKDDRQLRTA
MISSTFWATMICLLLSLIFLGFGSDLIQMITNIPSVQATAEHYLPWLVAMPLIAVWCFLLDGVFIGATKGKEMRNSMAVS
AVAFFAIYGFMTHYGNHALWLAMLSFMALRGFTLGATFYTQWRAGTFLPARFSI

Specific function: Unknown

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790477, Length=428, Percent_Identity=50.4672897196262, Blast_Score=352, Evalue=4e-98,
Organism=Escherichia coli, GI226510954, Length=453, Percent_Identity=24.7240618101545, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49711; Mature: 49711

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFCVSLILQTLKQPNVHRQVLAIALPMVLSNITVPLLGLVDAAVIGHLEHAWYLGGVALG
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STMISVTFWLLGFLRMSTTGLTAQAHGAQNSQQLARVLLQGSVIALGLAALFLLFHRPVA
HHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
ELIFHFSDASSEVKTYAEAYFYIRAWSAPAALLNFVLLGWLLGTQNARAPMWMVIITNLT
HHHHHHCCCHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
NIVLDLLLVLGLGLKVEGAAIASVIADYAGLLFGLLCVGRYWRQHQLPAPFSFIPSLTKE
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LSRLVALNRDIFLRSLCLQAVFSFMTFQGAALGDDIVAANAVLMSFLMMISYGMDGFAYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
MEAMVGKAIGAKDDRQLRTAMISSTFWATMICLLLSLIFLGFGSDLIQMITNIPSVQATA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHH
EHYLPWLVAMPLIAVWCFLLDGVFIGATKGKEMRNSMAVSAVAFFAIYGFMTHYGNHALW
HHHHHHHHHHHHHHHHHHHHCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LAMLSFMALRGFTLGATFYTQWRAGTFLPARFSI
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503 [H]