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The map label for this gene is uspA [H]

Identifier: 227080312

GI number: 227080312

Start: 76175

End: 76600

Strand: Reverse

Name: uspA [H]

Synonym: VCM66_0076

Alternate gene names: 227080312

Gene position: 76600-76175 (Counterclockwise)

Preceding gene: 227080317

Following gene: 227080311

Centisome position: 2.65

GC content: 48.59

Gene sequence:

>426_bases
ATGAGTTATCAACATCTTTTGGTTGCGGTCGACCTGTCTGAAGACAGCAAACTGCTGGTTGAAAAAGCGGTGGCTCTGGC
CAAACCTCTCAATGCGGAAGTCTCATTTATTCATATCGACGTGAACTACGCCGAGCTGTACACGGGCCTTATCGACATTA
ATTTGGCCGAAACGCAACATCACGCGATGGAAGCTTCACAAAAGCAACTGCAGGATTTGGCGCAGCATGCCAATTACCCC
ATCAAGCATACCTTGGTCGGTAGCGGCGATTTAACCAATGAGCTGTGCGAAACCATCAGCGAGTTTAACGTGGATTTACT
GGTGTGTGGCCATCACCAAGATTTCTGGAGCAAACTGCTCTCTTCCACACGGCAACTGATCAACAGCTCGCCAGTGGATA
TGTTAGTCATTCCACTCAACGACTGA

Upstream 100 bases:

>100_bases
CATCACCTAACCCATCGCCATCAAACTGAGATCGCTGCGACAAAAAAATCGCCACTCCAGTGCTACGCTGTGTTTAGTCA
CCCGAATAGGAGTCAGTACA

Downstream 100 bases:

>100_bases
CGAACAGAAAATACTCAGGTAGACTGCTGATTTCCATTCACTCATTCAATAGCGTTATGGTTTATCTTTCAGCAGTCACC
CTGTTATGGGCATTTTCATT

Product: universal stress protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 141; Mature: 140

Protein sequence:

>141_residues
MSYQHLLVAVDLSEDSKLLVEKAVALAKPLNAEVSFIHIDVNYAELYTGLIDINLAETQHHAMEASQKQLQDLAQHANYP
IKHTLVGSGDLTNELCETISEFNVDLLVCGHHQDFWSKLLSSTRQLINSSPVDMLVIPLND

Sequences:

>Translated_141_residues
MSYQHLLVAVDLSEDSKLLVEKAVALAKPLNAEVSFIHIDVNYAELYTGLIDINLAETQHHAMEASQKQLQDLAQHANYP
IKHTLVGSGDLTNELCETISEFNVDLLVCGHHQDFWSKLLSSTRQLINSSPVDMLVIPLND
>Mature_140_residues
SYQHLLVAVDLSEDSKLLVEKAVALAKPLNAEVSFIHIDVNYAELYTGLIDINLAETQHHAMEASQKQLQDLAQHANYPI
KHTLVGSGDLTNELCETISEFNVDLLVCGHHQDFWSKLLSSTRQLINSSPVDMLVIPLND

Specific function: Required for resistance to DNA-damaging agents [H]

COG id: COG0589

COG function: function code T; Universal stress protein UspA and related nucleotide-binding proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the universal stress protein A family [H]

Homologues:

Organism=Escherichia coli, GI1789909, Length=141, Percent_Identity=58.8652482269503, Blast_Score=184, Evalue=2e-48,
Organism=Escherichia coli, GI1790358, Length=141, Percent_Identity=32.6241134751773, Blast_Score=87, Evalue=3e-19,
Organism=Escherichia coli, GI1788205, Length=141, Percent_Identity=34.0425531914894, Blast_Score=87, Evalue=6e-19,

Paralogues:

None

Copy number: 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1580 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 290 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4003 Molecules/Cell In: Growth Phase, G

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR006016 [H]

Pfam domain/function: PF00582 Usp [H]

EC number: NA

Molecular weight: Translated: 15707; Mature: 15576

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYQHLLVAVDLSEDSKLLVEKAVALAKPLNAEVSFIHIDVNYAELYTGLIDINLAETQH
CCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHEEEEHHHHH
HAMEASQKQLQDLAQHANYPIKHTLVGSGDLTNELCETISEFNVDLLVCGHHQDFWSKLL
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCEEEEEECCCHHHHHHHH
SSTRQLINSSPVDMLVIPLND
HHHHHHHCCCCCCEEEEEECC
>Mature Secondary Structure 
SYQHLLVAVDLSEDSKLLVEKAVALAKPLNAEVSFIHIDVNYAELYTGLIDINLAETQH
CCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHEEEEHHHHH
HAMEASQKQLQDLAQHANYPIKHTLVGSGDLTNELCETISEFNVDLLVCGHHQDFWSKLL
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCEEEEEECCCHHHHHHHH
SSTRQLINSSPVDMLVIPLND
HHHHHHHCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]