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The map label for this gene is glyS

Identifier: 227080257

GI number: 227080257

Start: 16869

End: 18935

Strand: Reverse

Name: glyS

Synonym: VCM66_0020

Alternate gene names: 227080257

Gene position: 18935-16869 (Counterclockwise)

Preceding gene: 227080258

Following gene: 227080256

Centisome position: 0.65

GC content: 52.06

Gene sequence:

>2067_bases
ATGGCAAAAGAATTTTTAATTGAGCTAGGCACAGAAGAGCTGCCACCAAAGCAACTGCGCACACTGGCTGAAGCCTTTGC
GGCGAATTTTGCGGCAGAACTGGCAACAGCTGACATCGCCCATGAAGGCGTAACTTGGTTTGCCACGCCACGCCGTTTGG
CACTGAAAGTGGCCAATTTGGCAGAAAGCCAACCGGATCGCGTCGTTGAAAAACGTGGTCCTGCCGTCAACGTTGCGTTT
GATGCCGATGGCAAGCCAACCAAAGCCGCTGAAGGCTGGGCGCGTGGCAATGGCATCACGGTAGAGCAAGCAGAACGTCT
CGTCACAGACAAAGGCGAATGGCTGCTCTTCAAAGAACAAGTGCAAGGCCAACAAACCGCTTCTGTGGTTGTGGAGATGG
CGGCCAAAGCGCTGGCTAACCTGCCGATTGCGAAACCGATGCGTTGGGGCGACAAAGAAACCCAATTTATCCGTCCAGTA
AAAACTCTGACCATTCTGTTTGGTAGCGAACTGATCCAAGGCGAAATCTTGGGTGTTGCTTCTGCACGCACCCTCCGTGG
TCACCGCTTTATGGGTGAAGCGGAATTCACCATCGAATCGGCTGAGCAGTACCCTGCGATCCTTGAAGAGCGCGGCAAAG
TCATCGCTGACTACGCGACTCGTAAAGCGATGATCATCGAAGGCTCACAACAAGCGGCTCAGCAGCTCGGCGGTATCGCG
GATCTGGAAGATGCGCTGGTAGAAGAAGTGACCTCGCTGGTGGAATGGCCAGTGGTGATGACGGCGAAATTTGAAGAAAA
ATTCCTCAAAGTTCCAGCAGAAGCTTTGGTGTACACCATGAAAGGTGACCAAAAATATTTCCCAGTCTATGACGCAAGCA
AAAAACTGCTGCCTAACTTCATCTTCGTTTCGAACATCGAATCGAAAGAGCCTCGCCACATTGTGGAAGGTAACGAGAAG
GTAGTTCGTCCACGTCTGGCAGATGCAGAGTTCTTCTTTAATACTGACCGCAAGCGCCCATTGGTGGATCGCTTACCTCT
GCTCGAAAACGCGATTTTCCAACAGCAACTCGGCACGATTAAAGACAAAACCGACCGCATTACTGAACTGGCTGGCTACA
TCGCAGAGCAGATCGGTGCTGACGTGGAGAAATCTAAGCGTGCAGGCCTACTGGCAAAATGTGACCTAATGACCTCGATG
GTGTTTGAATTTACCGACACCCAAGGCGTGATGGGTATGCATTATGCGCGTCACGATGGCGAAGCAGAAGAAGTTGCAGT
TGCGCTGAATGAGCAGTACATGCCACGTTTCGCGGGTGATGAACTGCCAAGCCGTGGTGTTTCAGCTGCGGTCGCGATGG
CTGACAAACTCGATACTATCGTCGGTATATTTGGTATTGGTCAGGCGCCGAAAGGCAGTGACCCATTTGCGCTGCGTCGT
GCTTCACTGGGTGTACTGCGTATTCTGGTTGAGTACGGCTACCAGTTGGATCTGGTGGATTTGATCGCCAAAGCCAAATC
ACTGTTTGGTGACCGCCTGACTAACGCCAACGTTGAGCAGGAAGTGATCGAGTTTATGCTTGGCCGTTTCCCAACCTGGT
ACCAAGATGCCGGTTTTAGCATCGACATCATCCAAGCGGTACTGGCGCGTAACCCAACCAAACCAGCCGACTTTGATCAG
CGTGTGAAAGCCGTATCGCACTTCCGCGCTCTTGAAGAAGCCGAAGCACTGGCTGCTGCCAACAAGCGTGTAGGCAACAT
TCTGGCTAAGTACGATGGTGAATTGGGTGAAGAAATCGATCTCGCTCTGCTGCAAGAAGATGCGGAAAAAGCCTTAGCCG
AAGCCGTCGAAATCATGGCAGAAGCGTTAGAACCTGCGTTTGCAACCGGCAACTACCAAGAAGCTCTGAGCAAACTGGCT
GGCCTGCGCGCGCCTGTCGATGCCTTCTTCGACAACGTGATGGTGATGGCTGACGATGAAGCACTGAAGAAGAACCGTCT
CACGCTACTCAACAAGCTGCGTAACCTGTTCCTGCAAATTGCCGACATTTCGCTGCTGCAAAAATAA

Upstream 100 bases:

>100_bases
ACCTAACCAAATCGGTCGCCGAAGCTTATTACGCATCCCGTGAAGCTCTGGGCTTCCCTATGTGTAAGAAGTCAGAACAG
AAGTAAGGGGAAGACGTCAC

Downstream 100 bases:

>100_bases
TCTGCAGCACGACAATTTCGTCAACAAACTTTAAGCCCAACTCAAAAGAGTTGGGCTTTGTTTTTGCTCGCGCTCCCCAA
ACGACTTGGAGTTGCAGGTA

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MAKEFLIELGTEELPPKQLRTLAEAFAANFAAELATADIAHEGVTWFATPRRLALKVANLAESQPDRVVEKRGPAVNVAF
DADGKPTKAAEGWARGNGITVEQAERLVTDKGEWLLFKEQVQGQQTASVVVEMAAKALANLPIAKPMRWGDKETQFIRPV
KTLTILFGSELIQGEILGVASARTLRGHRFMGEAEFTIESAEQYPAILEERGKVIADYATRKAMIIEGSQQAAQQLGGIA
DLEDALVEEVTSLVEWPVVMTAKFEEKFLKVPAEALVYTMKGDQKYFPVYDASKKLLPNFIFVSNIESKEPRHIVEGNEK
VVRPRLADAEFFFNTDRKRPLVDRLPLLENAIFQQQLGTIKDKTDRITELAGYIAEQIGADVEKSKRAGLLAKCDLMTSM
VFEFTDTQGVMGMHYARHDGEAEEVAVALNEQYMPRFAGDELPSRGVSAAVAMADKLDTIVGIFGIGQAPKGSDPFALRR
ASLGVLRILVEYGYQLDLVDLIAKAKSLFGDRLTNANVEQEVIEFMLGRFPTWYQDAGFSIDIIQAVLARNPTKPADFDQ
RVKAVSHFRALEEAEALAAANKRVGNILAKYDGELGEEIDLALLQEDAEKALAEAVEIMAEALEPAFATGNYQEALSKLA
GLRAPVDAFFDNVMVMADDEALKKNRLTLLNKLRNLFLQIADISLLQK

Sequences:

>Translated_688_residues
MAKEFLIELGTEELPPKQLRTLAEAFAANFAAELATADIAHEGVTWFATPRRLALKVANLAESQPDRVVEKRGPAVNVAF
DADGKPTKAAEGWARGNGITVEQAERLVTDKGEWLLFKEQVQGQQTASVVVEMAAKALANLPIAKPMRWGDKETQFIRPV
KTLTILFGSELIQGEILGVASARTLRGHRFMGEAEFTIESAEQYPAILEERGKVIADYATRKAMIIEGSQQAAQQLGGIA
DLEDALVEEVTSLVEWPVVMTAKFEEKFLKVPAEALVYTMKGDQKYFPVYDASKKLLPNFIFVSNIESKEPRHIVEGNEK
VVRPRLADAEFFFNTDRKRPLVDRLPLLENAIFQQQLGTIKDKTDRITELAGYIAEQIGADVEKSKRAGLLAKCDLMTSM
VFEFTDTQGVMGMHYARHDGEAEEVAVALNEQYMPRFAGDELPSRGVSAAVAMADKLDTIVGIFGIGQAPKGSDPFALRR
ASLGVLRILVEYGYQLDLVDLIAKAKSLFGDRLTNANVEQEVIEFMLGRFPTWYQDAGFSIDIIQAVLARNPTKPADFDQ
RVKAVSHFRALEEAEALAAANKRVGNILAKYDGELGEEIDLALLQEDAEKALAEAVEIMAEALEPAFATGNYQEALSKLA
GLRAPVDAFFDNVMVMADDEALKKNRLTLLNKLRNLFLQIADISLLQK
>Mature_687_residues
AKEFLIELGTEELPPKQLRTLAEAFAANFAAELATADIAHEGVTWFATPRRLALKVANLAESQPDRVVEKRGPAVNVAFD
ADGKPTKAAEGWARGNGITVEQAERLVTDKGEWLLFKEQVQGQQTASVVVEMAAKALANLPIAKPMRWGDKETQFIRPVK
TLTILFGSELIQGEILGVASARTLRGHRFMGEAEFTIESAEQYPAILEERGKVIADYATRKAMIIEGSQQAAQQLGGIAD
LEDALVEEVTSLVEWPVVMTAKFEEKFLKVPAEALVYTMKGDQKYFPVYDASKKLLPNFIFVSNIESKEPRHIVEGNEKV
VRPRLADAEFFFNTDRKRPLVDRLPLLENAIFQQQLGTIKDKTDRITELAGYIAEQIGADVEKSKRAGLLAKCDLMTSMV
FEFTDTQGVMGMHYARHDGEAEEVAVALNEQYMPRFAGDELPSRGVSAAVAMADKLDTIVGIFGIGQAPKGSDPFALRRA
SLGVLRILVEYGYQLDLVDLIAKAKSLFGDRLTNANVEQEVIEFMLGRFPTWYQDAGFSIDIIQAVLARNPTKPADFDQR
VKAVSHFRALEEAEALAAANKRVGNILAKYDGELGEEIDLALLQEDAEKALAEAVEIMAEALEPAFATGNYQEALSKLAG
LRAPVDAFFDNVMVMADDEALKKNRLTLLNKLRNLFLQIADISLLQK

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=686, Percent_Identity=68.5131195335277, Blast_Score=957, Evalue=0.0,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_VIBC3 (A5F487)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- STRING:   A5F487
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2499
- eggNOG:   COG0751
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- ProtClustDB:   PRK01233
- BioCyc:   VCHO345073:VC0395_A2499-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- SMART:   SM00836
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 75926; Mature: 75795

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEFLIELGTEELPPKQLRTLAEAFAANFAAELATADIAHEGVTWFATPRRLALKVANL
CCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHH
AESQPDRVVEKRGPAVNVAFDADGKPTKAAEGWARGNGITVEQAERLVTDKGEWLLFKEQ
HCCCCHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEHHH
VQGQQTASVVVEMAAKALANLPIAKPMRWGDKETQFIRPVKTLTILFGSELIQGEILGVA
HCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SARTLRGHRFMGEAEFTIESAEQYPAILEERGKVIADYATRKAMIIEGSQQAAQQLGGIA
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEHHHHCEEEEEECCHHHHHHHCCCC
DLEDALVEEVTSLVEWPVVMTAKFEEKFLKVPAEALVYTMKGDQKYFPVYDASKKLLPNF
HHHHHHHHHHHHHHHCCHHEEHHHHHHHHHCCHHHEEEEECCCCEECCCCCCCHHHCCCE
IFVSNIESKEPRHIVEGNEKVVRPRLADAEFFFNTDRKRPLVDRLPLLENAIFQQQLGTI
EEEECCCCCCCCHHHCCCCCEECCCCCCCHHEECCCCCCCHHHHHHHHHHHHHHHHHCCH
KDKTDRITELAGYIAEQIGADVEKSKRAGLLAKCDLMTSMVFEFTDTQGVMGMHYARHDG
HHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCC
EAEEVAVALNEQYMPRFAGDELPSRGVSAAVAMADKLDTIVGIFGIGQAPKGSDPFALRR
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
ASLGVLRILVEYGYQLDLVDLIAKAKSLFGDRLTNANVEQEVIEFMLGRFPTWYQDAGFS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHCCCCC
IDIIQAVLARNPTKPADFDQRVKAVSHFRALEEAEALAAANKRVGNILAKYDGELGEEID
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
LALLQEDAEKALAEAVEIMAEALEPAFATGNYQEALSKLAGLRAPVDAFFDNVMVMADDE
HHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHCCCCCHHHHHCCEEEEECCH
ALKKNRLTLLNKLRNLFLQIADISLLQK
HHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKEFLIELGTEELPPKQLRTLAEAFAANFAAELATADIAHEGVTWFATPRRLALKVANL
CHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHH
AESQPDRVVEKRGPAVNVAFDADGKPTKAAEGWARGNGITVEQAERLVTDKGEWLLFKEQ
HCCCCHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEHHH
VQGQQTASVVVEMAAKALANLPIAKPMRWGDKETQFIRPVKTLTILFGSELIQGEILGVA
HCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SARTLRGHRFMGEAEFTIESAEQYPAILEERGKVIADYATRKAMIIEGSQQAAQQLGGIA
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEHHHHCEEEEEECCHHHHHHHCCCC
DLEDALVEEVTSLVEWPVVMTAKFEEKFLKVPAEALVYTMKGDQKYFPVYDASKKLLPNF
HHHHHHHHHHHHHHHCCHHEEHHHHHHHHHCCHHHEEEEECCCCEECCCCCCCHHHCCCE
IFVSNIESKEPRHIVEGNEKVVRPRLADAEFFFNTDRKRPLVDRLPLLENAIFQQQLGTI
EEEECCCCCCCCHHHCCCCCEECCCCCCCHHEECCCCCCCHHHHHHHHHHHHHHHHHCCH
KDKTDRITELAGYIAEQIGADVEKSKRAGLLAKCDLMTSMVFEFTDTQGVMGMHYARHDG
HHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCC
EAEEVAVALNEQYMPRFAGDELPSRGVSAAVAMADKLDTIVGIFGIGQAPKGSDPFALRR
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
ASLGVLRILVEYGYQLDLVDLIAKAKSLFGDRLTNANVEQEVIEFMLGRFPTWYQDAGFS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHCCCCC
IDIIQAVLARNPTKPADFDQRVKAVSHFRALEEAEALAAANKRVGNILAKYDGELGEEID
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
LALLQEDAEKALAEAVEIMAEALEPAFATGNYQEALSKLAGLRAPVDAFFDNVMVMADDE
HHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHCCCCCHHHHHCCEEEEECCH
ALKKNRLTLLNKLRNLFLQIADISLLQK
HHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA