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The map label for this gene is dnaB [H]

Identifier: 226951072

GI number: 226951072

Start: 4131157

End: 4132494

Strand: Reverse

Name: dnaB [H]

Synonym: CLM_4125

Alternate gene names: 226951072

Gene position: 4132494-4131157 (Counterclockwise)

Preceding gene: 226951073

Following gene: 226951071

Centisome position: 99.45

GC content: 33.48

Gene sequence:

>1338_bases
ATGGATGCACCCATAAAAAGTATGCCGCAGAGTATAGATGCAGAGCAAAATGTATTAGGGGCAATGATTATAGATAAAAC
TTCCATAGCAGAGGCTGTGGAAGTTTTAAAAAGTGAAGATTTTTATAAAGATTCTCATAAAATTATATTTAGTGGAATTA
TAGAGCTTTATCAAAAGGATATAGCAGTAGATATGCTAACTCTTACGGAAAATCTTAAGTCACGAGATAAACTAGATGCA
GTAGGTGGGGTTACTTATATAACGGAGCTTTGTAACTCTATAGTTTCTACAGCTAATATTCAGTCTTATATAGATATAAT
AAAGGATAAATCTACATTAAGAAGGCTTATAAAGTCATCTACTGAAATAATAGAGAACTGTTATAACAGACAGGATAATG
TAGAAGAAATAATAGATTCTGCAGAGAAAAAAATATTTCACATATCCAACCAAAATACTACAACTGATTTTGAACCATTA
AGTAATGTTTTGGAAAGAGGATTTATTCAAATTGAAAACTTATTTAAAAATAAAGGTGAAACTACAGGGGTTGCATCTGG
TTTTAGAGAACTAGACGCTAAAACCTCAGGTTTTCAAAAGTCTGATATGATATTAATAGCAGCAAGACCATCAATGGGTA
AAACTACATTTGCTCTAAACATAGCAGAATATGCAGCTCTTAGAGAGGGAAAAAGTGTAGCAATCTTTTCCCTGGAAATG
TCAAAAGAGCAACTTTCTTATAAATTGCTTTGTTCCCAAGCTAATATTGATATGTTGAGGCTTAGAACTGGTAACTTAGA
GGATAAAGACTGGGAGAATATAGCTAGGGTTTCAGGGCCTTTAGCAGCAGCAAAGATATTTATAGATGATACTGCAGGTA
TGTCTGTAATGGAAATGCGTTCTAAATGTAGGAGATTAAAAATAGAACATGGAATAGATATGGTTTTAATAGATTATCTT
CAGCTTATGAGTGGAGGTAAAGGGTCTACTGAAAGTAGACAACAAGAAGTCTCAGAAATATCTAGATCTATAAAAGCCTT
AGCTAAAGAAATGGATTGTCCAGTTATAGCCTTATCTCAATTATCTCGTGCTCCGGAGGCTAGATCGGATCATAGACCAA
TGCTTTCAGATCTTAGAGAATCTGGATCTATAGAACAGGATGCTGACGTAGTTATGTTTTTATATAGAGATGAATACTAT
GACAAAGAAACAGAAGATAAAAACATGGCAGAATGCATTATAGCCAAGCAAAGAAATGGTCCTACGGGTACAGTTAAAAT
GGCATGGCTTGGACAATACAGTAAATTTGGTAATTTAGATGTGATACATCAAGAATAA

Upstream 100 bases:

>100_bases
TTCAGATATAAAAGATATAGAAATAATATGTATAAATACTATAGAAGAGCTTATAAATATAGTATTTTAAATTGAGGAGT
ACTTAAGGAGAGATGTTTAT

Downstream 100 bases:

>100_bases
AACATACAAAGTCCTATATTTATTTTATAGGGCTTTTATTTTTTCGCATTCTTTATAATTCATTTATTTTAAGATTATAT
TTATATTAATTTATAATAGG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MDAPIKSMPQSIDAEQNVLGAMIIDKTSIAEAVEVLKSEDFYKDSHKIIFSGIIELYQKDIAVDMLTLTENLKSRDKLDA
VGGVTYITELCNSIVSTANIQSYIDIIKDKSTLRRLIKSSTEIIENCYNRQDNVEEIIDSAEKKIFHISNQNTTTDFEPL
SNVLERGFIQIENLFKNKGETTGVASGFRELDAKTSGFQKSDMILIAARPSMGKTTFALNIAEYAALREGKSVAIFSLEM
SKEQLSYKLLCSQANIDMLRLRTGNLEDKDWENIARVSGPLAAAKIFIDDTAGMSVMEMRSKCRRLKIEHGIDMVLIDYL
QLMSGGKGSTESRQQEVSEISRSIKALAKEMDCPVIALSQLSRAPEARSDHRPMLSDLRESGSIEQDADVVMFLYRDEYY
DKETEDKNMAECIIAKQRNGPTGTVKMAWLGQYSKFGNLDVIHQE

Sequences:

>Translated_445_residues
MDAPIKSMPQSIDAEQNVLGAMIIDKTSIAEAVEVLKSEDFYKDSHKIIFSGIIELYQKDIAVDMLTLTENLKSRDKLDA
VGGVTYITELCNSIVSTANIQSYIDIIKDKSTLRRLIKSSTEIIENCYNRQDNVEEIIDSAEKKIFHISNQNTTTDFEPL
SNVLERGFIQIENLFKNKGETTGVASGFRELDAKTSGFQKSDMILIAARPSMGKTTFALNIAEYAALREGKSVAIFSLEM
SKEQLSYKLLCSQANIDMLRLRTGNLEDKDWENIARVSGPLAAAKIFIDDTAGMSVMEMRSKCRRLKIEHGIDMVLIDYL
QLMSGGKGSTESRQQEVSEISRSIKALAKEMDCPVIALSQLSRAPEARSDHRPMLSDLRESGSIEQDADVVMFLYRDEYY
DKETEDKNMAECIIAKQRNGPTGTVKMAWLGQYSKFGNLDVIHQE
>Mature_445_residues
MDAPIKSMPQSIDAEQNVLGAMIIDKTSIAEAVEVLKSEDFYKDSHKIIFSGIIELYQKDIAVDMLTLTENLKSRDKLDA
VGGVTYITELCNSIVSTANIQSYIDIIKDKSTLRRLIKSSTEIIENCYNRQDNVEEIIDSAEKKIFHISNQNTTTDFEPL
SNVLERGFIQIENLFKNKGETTGVASGFRELDAKTSGFQKSDMILIAARPSMGKTTFALNIAEYAALREGKSVAIFSLEM
SKEQLSYKLLCSQANIDMLRLRTGNLEDKDWENIARVSGPLAAAKIFIDDTAGMSVMEMRSKCRRLKIEHGIDMVLIDYL
QLMSGGKGSTESRQQEVSEISRSIKALAKEMDCPVIALSQLSRAPEARSDHRPMLSDLRESGSIEQDADVVMFLYRDEYY
DKETEDKNMAECIIAKQRNGPTGTVKMAWLGQYSKFGNLDVIHQE

Specific function: Participates In Initiation And Elongation During Chromosome Replication; It Exhibits DNA-Dependent ATPase Activity And Contains Distinct Active Sites For ATP Binding, DNA Binding, And Interaction With DNAc Protein, Primase, And Other Prepriming Proteins.

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=445, Percent_Identity=44.9438202247191, Blast_Score=393, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 49891; Mature: 49891

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAPIKSMPQSIDAEQNVLGAMIIDKTSIAEAVEVLKSEDFYKDSHKIIFSGIIELYQKD
CCCCHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
IAVDMLTLTENLKSRDKLDAVGGVTYITELCNSIVSTANIQSYIDIIKDKSTLRRLIKSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
TEIIENCYNRQDNVEEIIDSAEKKIFHISNQNTTTDFEPLSNVLERGFIQIENLFKNKGE
HHHHHHHHCCCCCHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
TTGVASGFRELDAKTSGFQKSDMILIAARPSMGKTTFALNIAEYAALREGKSVAIFSLEM
CCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEEEEEHHHHHHHHCCCEEEEEEEEC
SKEQLSYKLLCSQANIDMLRLRTGNLEDKDWENIARVSGPLAAAKIFIDDTAGMSVMEMR
CHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHCCCHHEEEEEEECCCCHHHHHHH
SKCRRLKIEHGIDMVLIDYLQLMSGGKGSTESRQQEVSEISRSIKALAKEMDCPVIALSQ
HHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LSRAPEARSDHRPMLSDLRESGSIEQDADVVMFLYRDEYYDKETEDKNMAECIIAKQRNG
HHCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEHHHHCCCCCCCCHHHHHHHHHCCCC
PTGTVKMAWLGQYSKFGNLDVIHQE
CCCEEEEEEECCCCCCCCEEEEECC
>Mature Secondary Structure
MDAPIKSMPQSIDAEQNVLGAMIIDKTSIAEAVEVLKSEDFYKDSHKIIFSGIIELYQKD
CCCCHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
IAVDMLTLTENLKSRDKLDAVGGVTYITELCNSIVSTANIQSYIDIIKDKSTLRRLIKSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
TEIIENCYNRQDNVEEIIDSAEKKIFHISNQNTTTDFEPLSNVLERGFIQIENLFKNKGE
HHHHHHHHCCCCCHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
TTGVASGFRELDAKTSGFQKSDMILIAARPSMGKTTFALNIAEYAALREGKSVAIFSLEM
CCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEEEEEHHHHHHHHCCCEEEEEEEEC
SKEQLSYKLLCSQANIDMLRLRTGNLEDKDWENIARVSGPLAAAKIFIDDTAGMSVMEMR
CHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHCCCHHEEEEEEECCCCHHHHHHH
SKCRRLKIEHGIDMVLIDYLQLMSGGKGSTESRQQEVSEISRSIKALAKEMDCPVIALSQ
HHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LSRAPEARSDHRPMLSDLRESGSIEQDADVVMFLYRDEYYDKETEDKNMAECIIAKQRNG
HHCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEHHHHCCCCCCCCHHHHHHHHHCCCC
PTGTVKMAWLGQYSKFGNLDVIHQE
CCCEEEEEEECCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1312531 [H]