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The map label for this gene is nfsA [C]

Identifier: 226951031

GI number: 226951031

Start: 4088794

End: 4089333

Strand: Reverse

Name: nfsA [C]

Synonym: CLM_4070

Alternate gene names: 226951031

Gene position: 4089333-4088794 (Counterclockwise)

Preceding gene: 226951040

Following gene: 226951027

Centisome position: 98.41

GC content: 33.89

Gene sequence:

>540_bases
ATGTTTCAAGAAATTAGATCAAGAAGAAGTATACGAAAATATATTGATAAGCCAATTGAAGACGAAAAAATAATGGAGAT
TATTGAGAGCGCTAGACTCGCCCCATCTGGTTCTAACACACAGCCTTGGCATTTTATTGTGGTAAAATCTGATATCACTA
GGGAAAAGTTAGCTAAGGTATCCCATAATCAAGGATGGATGATGGATGCGCCAGTTTTTATTGCATGTGTTGCAGATATA
CGATCTAGGATTAAAGAAGATATAGAACTATCATTGAATGAAAATAGCCCGCAACAAGAACTTAAGCAAATAATAAGAGA
CACATCAATAGCTGTAGAACATTTAGTACTTTCCGCCGAAAACTTAGGTTTAGGCACTTGTTGGATTGCATGGTTTACTC
AAGAAGAAGTTAGGCCAATTTTAAATATACCTTCTGATAAATATGTAGTTAGTATTATTACACTTGGTTACCCAAATGAA
TCACCTAAAACTAGGCCAAGAAAAAAATTACAAGATATAATACATTATGAACAGTGGTAA

Upstream 100 bases:

>100_bases
TGATACAAATAAGTGCGTACTGGTAATAGTAATACATATATTCTAGAATAATGTTATATATTAAAAATAAAATTAATAGA
ACAAGGAGGATTCTAATAAT

Downstream 100 bases:

>100_bases
AGAATATATTTATATTAAATTTAATGCTGCAATTAAAAATTATAGTGATTAATATTTTTAATTGCAGCATTAAAATTTAT
ATAATGAAGTTAGTATGCCA

Product: nitroreductase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MFQEIRSRRSIRKYIDKPIEDEKIMEIIESARLAPSGSNTQPWHFIVVKSDITREKLAKVSHNQGWMMDAPVFIACVADI
RSRIKEDIELSLNENSPQQELKQIIRDTSIAVEHLVLSAENLGLGTCWIAWFTQEEVRPILNIPSDKYVVSIITLGYPNE
SPKTRPRKKLQDIIHYEQW

Sequences:

>Translated_179_residues
MFQEIRSRRSIRKYIDKPIEDEKIMEIIESARLAPSGSNTQPWHFIVVKSDITREKLAKVSHNQGWMMDAPVFIACVADI
RSRIKEDIELSLNENSPQQELKQIIRDTSIAVEHLVLSAENLGLGTCWIAWFTQEEVRPILNIPSDKYVVSIITLGYPNE
SPKTRPRKKLQDIIHYEQW
>Mature_179_residues
MFQEIRSRRSIRKYIDKPIEDEKIMEIIESARLAPSGSNTQPWHFIVVKSDITREKLAKVSHNQGWMMDAPVFIACVADI
RSRIKEDIELSLNENSPQQELKQIIRDTSIAVEHLVLSAENLGLGTCWIAWFTQEEVRPILNIPSDKYVVSIITLGYPNE
SPKTRPRKKLQDIIHYEQW

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=175, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 20798; Mature: 20798

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQEIRSRRSIRKYIDKPIEDEKIMEIIESARLAPSGSNTQPWHFIVVKSDITREKLAKV
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHH
SHNQGWMMDAPVFIACVADIRSRIKEDIELSLNENSPQQELKQIIRDTSIAVEHLVLSAE
CCCCCCEECCHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHC
NLGLGTCWIAWFTQEEVRPILNIPSDKYVVSIITLGYPNESPKTRPRKKLQDIIHYEQW
CCCCHHHHHEECCHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MFQEIRSRRSIRKYIDKPIEDEKIMEIIESARLAPSGSNTQPWHFIVVKSDITREKLAKV
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHH
SHNQGWMMDAPVFIACVADIRSRIKEDIELSLNENSPQQELKQIIRDTSIAVEHLVLSAE
CCCCCCEECCHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHC
NLGLGTCWIAWFTQEEVRPILNIPSDKYVVSIITLGYPNESPKTRPRKKLQDIIHYEQW
CCCCHHHHHEECCHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]