BacMap

Definition	Clostridium botulinum A2 str. Kyoto chromosome, complete genome.
Accession	NC_012563
Length	4,155,278

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The map label for this gene is ppiA [C]

Identifier: 226951017

GI number: 226951017

Start: 4075110

End: 4075628

Strand: Reverse

Name: ppiA [C]

Synonym: CLM_4054

Alternate gene names: 226951017

Gene position: 4075628-4075110 (Counterclockwise)

Preceding gene: 226951018

Following gene: 226951016

Centisome position: 98.08

GC content: 31.41

Gene sequence:

>519_bases
ATGAATCCTATAGTATCTATAAAAATGAGTAATGGAAAAGAAATGAAAATAGAATTATATCCAGAAATAGCACCTAATAC
AGTTGACAATTTTATATCATTAGTAAATAAAGGTTTTTATAATGGTATTATATTCCACAGAGTAATTCCAGGATTTATGA
TTCAGGGAGGTTGTCCTAACGGAAACGGAATGGGAGGCCCAGGATATTCAATAAAAGGTGAATTTAAATCTAATGGATTT
AACAATGAGTTAAAACATACAGAAGGTGTTATATCAATGGCCAGAACAATGCAACCAAATTCTGCAGGAAGTCAATTCTT
TATAATGGTTGCTGATGCACCACATTTAGATAACCAATATGCAGCCTTTGGAAAAGTTATAGAAGGACTAGATGTTGCCA
AAGAAATAGTAAGTGTAGATAGAGATTTTAAAGATAAACCTTTTGAAGATCAAGTTATGAAGACTGTAACAGTAGATACC
TTTGGAGAAGAATTTCATCAGCCAGAAAAATTAAGTTAA

Upstream 100 bases:

>100_bases
CATAAAAAACAGAGTATAATAAAGATATTAAAATATGATATAATTTATGCTAATATGAAATATATGGAACAAAATTATTG
TTTTGAGAGGGGAAATAGAA

Downstream 100 bases:

>100_bases
AAAGGAGGCAGTTTGTTTTGATTAATAATAAGTTGATGCTTGTTTATGGATTAAAAGAAGCAGAGTTAAACTTGTTAAAA
GATATGTGCGCTAATAATAA

Product: putative cyclophilin-type peptidyl-prolyl cis-trans isomerase

Products: NA

Alternate protein names: Phosphatase; Peptidyl-prolyl cis-trans isomerase; PPIase; Rotamase [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MNPIVSIKMSNGKEMKIELYPEIAPNTVDNFISLVNKGFYNGIIFHRVIPGFMIQGGCPNGNGMGGPGYSIKGEFKSNGF
NNELKHTEGVISMARTMQPNSAGSQFFIMVADAPHLDNQYAAFGKVIEGLDVAKEIVSVDRDFKDKPFEDQVMKTVTVDT
FGEEFHQPEKLS

Sequences:

>Translated_172_residues
MNPIVSIKMSNGKEMKIELYPEIAPNTVDNFISLVNKGFYNGIIFHRVIPGFMIQGGCPNGNGMGGPGYSIKGEFKSNGF
NNELKHTEGVISMARTMQPNSAGSQFFIMVADAPHLDNQYAAFGKVIEGLDVAKEIVSVDRDFKDKPFEDQVMKTVTVDT
FGEEFHQPEKLS
>Mature_172_residues
MNPIVSIKMSNGKEMKIELYPEIAPNTVDNFISLVNKGFYNGIIFHRVIPGFMIQGGCPNGNGMGGPGYSIKGEFKSNGF
NNELKHTEGVISMARTMQPNSAGSQFFIMVADAPHLDNQYAAFGKVIEGLDVAKEIVSVDRDFKDKPFEDQVMKTVTVDT
FGEEFHQPEKLS

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI19557636, Length=147, Percent_Identity=43.5374149659864, Blast_Score=114, Evalue=4e-26,
Organism=Homo sapiens, GI24308049, Length=147, Percent_Identity=42.8571428571429, Blast_Score=108, Evalue=3e-24,
Organism=Homo sapiens, GI7706339, Length=135, Percent_Identity=40, Blast_Score=101, Evalue=3e-22,
Organism=Homo sapiens, GI4758950, Length=141, Percent_Identity=43.2624113475177, Blast_Score=91, Evalue=3e-19,
Organism=Homo sapiens, GI22547212, Length=154, Percent_Identity=40.9090909090909, Blast_Score=90, Evalue=8e-19,
Organism=Homo sapiens, GI7657473, Length=154, Percent_Identity=40.9090909090909, Blast_Score=90, Evalue=8e-19,
Organism=Homo sapiens, GI64276486, Length=127, Percent_Identity=39.3700787401575, Blast_Score=88, Evalue=5e-18,
Organism=Homo sapiens, GI5031987, Length=147, Percent_Identity=44.8979591836735, Blast_Score=87, Evalue=7e-18,
Organism=Homo sapiens, GI22547215, Length=154, Percent_Identity=40.9090909090909, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI5454154, Length=161, Percent_Identity=37.2670807453416, Blast_Score=84, Evalue=4e-17,
Organism=Homo sapiens, GI10863927, Length=128, Percent_Identity=45.3125, Blast_Score=84, Evalue=5e-17,
Organism=Homo sapiens, GI42560244, Length=147, Percent_Identity=39.4557823129252, Blast_Score=84, Evalue=8e-17,
Organism=Homo sapiens, GI6631100, Length=145, Percent_Identity=37.2413793103448, Blast_Score=83, Evalue=9e-17,
Organism=Homo sapiens, GI4505991, Length=143, Percent_Identity=41.958041958042, Blast_Score=83, Evalue=1e-16,
Organism=Homo sapiens, GI4826932, Length=138, Percent_Identity=42.0289855072464, Blast_Score=83, Evalue=1e-16,
Organism=Homo sapiens, GI45439316, Length=144, Percent_Identity=43.75, Blast_Score=83, Evalue=1e-16,
Organism=Homo sapiens, GI303305009, Length=144, Percent_Identity=43.75, Blast_Score=83, Evalue=1e-16,
Organism=Homo sapiens, GI5174637, Length=144, Percent_Identity=43.75, Blast_Score=82, Evalue=2e-16,
Organism=Homo sapiens, GI256017253, Length=126, Percent_Identity=45.2380952380952, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI221139811, Length=126, Percent_Identity=45.2380952380952, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI256017251, Length=126, Percent_Identity=45.2380952380952, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI310127646, Length=147, Percent_Identity=40.1360544217687, Blast_Score=80, Evalue=8e-16,
Organism=Homo sapiens, GI310114371, Length=147, Percent_Identity=40.1360544217687, Blast_Score=80, Evalue=8e-16,
Organism=Homo sapiens, GI310119221, Length=147, Percent_Identity=40.1360544217687, Blast_Score=80, Evalue=8e-16,
Organism=Homo sapiens, GI178057341, Length=130, Percent_Identity=43.0769230769231, Blast_Score=79, Evalue=2e-15,
Organism=Homo sapiens, GI310110104, Length=144, Percent_Identity=40.9722222222222, Blast_Score=78, Evalue=3e-15,
Organism=Homo sapiens, GI150418007, Length=146, Percent_Identity=40.4109589041096, Blast_Score=78, Evalue=4e-15,
Organism=Homo sapiens, GI310129267, Length=144, Percent_Identity=40.2777777777778, Blast_Score=77, Evalue=8e-15,
Organism=Homo sapiens, GI310120620, Length=144, Percent_Identity=40.2777777777778, Blast_Score=77, Evalue=8e-15,
Organism=Homo sapiens, GI209417936, Length=128, Percent_Identity=42.96875, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI30102944, Length=128, Percent_Identity=42.96875, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI219842199, Length=128, Percent_Identity=42.96875, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI310132444, Length=130, Percent_Identity=40.7692307692308, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI310113152, Length=130, Percent_Identity=40.7692307692308, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI20911035, Length=145, Percent_Identity=35.8620689655172, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI169163387, Length=145, Percent_Identity=38.6206896551724, Blast_Score=69, Evalue=2e-12,
Organism=Homo sapiens, GI169164185, Length=145, Percent_Identity=38.6206896551724, Blast_Score=69, Evalue=2e-12,
Organism=Homo sapiens, GI169163623, Length=145, Percent_Identity=38.6206896551724, Blast_Score=69, Evalue=2e-12,
Organism=Homo sapiens, GI27735045, Length=144, Percent_Identity=35.4166666666667, Blast_Score=66, Evalue=1e-11,
Organism=Homo sapiens, GI14277126, Length=153, Percent_Identity=33.9869281045752, Blast_Score=66, Evalue=1e-11,
Organism=Homo sapiens, GI289803023, Length=167, Percent_Identity=30.5389221556886, Blast_Score=65, Evalue=3e-11,
Organism=Homo sapiens, GI310114540, Length=167, Percent_Identity=34.1317365269461, Blast_Score=64, Evalue=5e-11,
Organism=Homo sapiens, GI310127745, Length=129, Percent_Identity=39.5348837209302, Blast_Score=64, Evalue=7e-11,
Organism=Homo sapiens, GI310119290, Length=129, Percent_Identity=39.5348837209302, Blast_Score=64, Evalue=7e-11,
Organism=Escherichia coli, GI1789763, Length=144, Percent_Identity=46.5277777777778, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1786736, Length=163, Percent_Identity=40.4907975460123, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17539496, Length=154, Percent_Identity=44.1558441558442, Blast_Score=116, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71980594, Length=155, Percent_Identity=42.5806451612903, Blast_Score=113, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI71980590, Length=148, Percent_Identity=43.2432432432432, Blast_Score=108, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17506311, Length=127, Percent_Identity=42.5196850393701, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17551282, Length=143, Percent_Identity=41.2587412587413, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17551284, Length=143, Percent_Identity=41.2587412587413, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17552780, Length=143, Percent_Identity=42.6573426573427, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17552782, Length=146, Percent_Identity=42.4657534246575, Blast_Score=87, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17506307, Length=152, Percent_Identity=40.7894736842105, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17565860, Length=145, Percent_Identity=42.0689655172414, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17532641, Length=129, Percent_Identity=37.2093023255814, Blast_Score=83, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17559074, Length=150, Percent_Identity=40.6666666666667, Blast_Score=83, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17566266, Length=133, Percent_Identity=43.609022556391, Blast_Score=83, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17539498, Length=125, Percent_Identity=42.4, Blast_Score=82, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71997782, Length=126, Percent_Identity=36.5079365079365, Blast_Score=81, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17552784, Length=150, Percent_Identity=38.6666666666667, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17532643, Length=162, Percent_Identity=36.4197530864198, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI32563659, Length=151, Percent_Identity=30.4635761589404, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6321848, Length=142, Percent_Identity=43.6619718309859, Blast_Score=86, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323562, Length=131, Percent_Identity=43.5114503816794, Blast_Score=85, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6320359, Length=128, Percent_Identity=43.75, Blast_Score=85, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6320510, Length=143, Percent_Identity=40.5594405594406, Blast_Score=80, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323246, Length=170, Percent_Identity=35.8823529411765, Blast_Score=75, Evalue=5e-15,
Organism=Drosophila melanogaster, GI17986117, Length=146, Percent_Identity=43.1506849315069, Blast_Score=116, Evalue=7e-27,
Organism=Drosophila melanogaster, GI19922376, Length=159, Percent_Identity=40.251572327044, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI45549139, Length=128, Percent_Identity=50, Blast_Score=97, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24652460, Length=147, Percent_Identity=37.4149659863946, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24664125, Length=128, Percent_Identity=46.875, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21357783, Length=128, Percent_Identity=46.875, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24650807, Length=148, Percent_Identity=36.4864864864865, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21357249, Length=115, Percent_Identity=38.2608695652174, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19922912, Length=139, Percent_Identity=43.1654676258993, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI20130249, Length=146, Percent_Identity=39.041095890411, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17647301, Length=123, Percent_Identity=43.9024390243902, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24661775, Length=151, Percent_Identity=36.4238410596026, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21355237, Length=151, Percent_Identity=36.4238410596026, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24661771, Length=151, Percent_Identity=36.4238410596026, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17136360, Length=146, Percent_Identity=35.6164383561644, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24586038, Length=163, Percent_Identity=34.9693251533742, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR023214
- InterPro:   IPR013200
- InterPro:   IPR006379
- InterPro:   IPR000150
- InterPro:   IPR002130 [H]

Pfam domain/function: PF08282 Hydrolase_3; PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 18985; Mature: 18985

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIVSIKMSNGKEMKIELYPEIAPNTVDNFISLVNKGFYNGIIFHRVIPGFMIQGGCPN
CCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHCHHHCCCHHHHHCCCEEEECCCCC
GNGMGGPGYSIKGEFKSNGFNNELKHTEGVISMARTMQPNSAGSQFFIMVADAPHLDNQY
CCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHH
AAFGKVIEGLDVAKEIVSVDRDFKDKPFEDQVMKTVTVDTFGEEFHQPEKLS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNPIVSIKMSNGKEMKIELYPEIAPNTVDNFISLVNKGFYNGIIFHRVIPGFMIQGGCPN
CCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHCHHHCCCHHHHHCCCEEEECCCCC
GNGMGGPGYSIKGEFKSNGFNNELKHTEGVISMARTMQPNSAGSQFFIMVADAPHLDNQY
CCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHH
AAFGKVIEGLDVAKEIVSVDRDFKDKPFEDQVMKTVTVDTFGEEFHQPEKLS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA