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The map label for this gene is fusA [H]

Identifier: 226950968

GI number: 226950968

Start: 4011659

End: 4013731

Strand: Reverse

Name: fusA [H]

Synonym: CLM_3985

Alternate gene names: 226950968

Gene position: 4013731-4011659 (Counterclockwise)

Preceding gene: 226950973

Following gene: 226950967

Centisome position: 96.59

GC content: 29.81

Gene sequence:

>2073_bases
ATGAATTATAGCACCACCAATCTAAGGAATGTAGGCATAATTGGACATAGTGGATCAGGAAAAACTTCTTTAACAGAAGC
TATTTTATATTTTACAAGCATGGTAGATAGATTAGGTACCATAGAAGAGGGAAATACTGTATCTGATTATGATACAGAGG
AAAAAAAACGTAAAATATCTATATCTACCACAATACAACCGTGTAAATGGAAAGACACAAAAATTAATTTGATTGATATG
CCTGGTTATTTTGATTTTGTAGGAGAAAAGATTCAAGGGTTAAAAGCTGCTGATATTGCTCTTATAGTATTGTCAGCAAC
AGGAAATATACATGTTGGAACAGAAAAATCTTGGAATTATACAACAAAAGAGAAAATACCTAAAGCATTTTATATAAACA
AATTAGATAAAGAAAATATGGATTTTGAAGGCACTTTAAATAAACTTAAAGAAAGATATGGTACATCTGTAGTACCTGTT
CAATACCCTATAAGAAAAGAAGAAAATTTTAAAGGTGTAATTAATGTTATTTCTAATAAAGCAACTATATTTAATGATAA
AACACATAAGATAGAAATAAAAGAAGTACCAAATGAATTATTAGATGAAGTTAATGAATGTAAAAATATGATTGTAGAAG
CAGTAGCTGAAACAGATGAGAAGCTTTTAGAAAAATATTTTGATGAAGGTACACTAAGTGAAAAGGATATATATGAAGGA
TTAATGGCAGGAACTGTAAAAGGGGAAATAGTTCCGGTAATGTGTGGGTCAGCTATAAAGGCTATAGGTATTGAAACTTT
TTTAGAGGATATAGTTCGATGGTTTCCATCACCAGCAGAAACAAAAAAAATGGAAGTATCAGATGGGATAAATAAAAATA
ATATAGAAATAAATTGTAATGAAAAAGAACCATTTTCAGCACTGGTTTTTAAAACTATTATAGATCCTTTTATAGGTAGA
TTATCATTATTCAAAGTAACGAGTGGAGTGTTAAATGAAGAGAGCTCTATTTATAATTCAACAAAAGATAAAAAGGAGAA
GGTAGGTAAATTATATTTCTTAAGGGGCAAGCAACAAATTCAAACAGATAGAGTAATTTCAGGAGATATAGGGGCAGTTA
GTAAATTGCAATATACCTTTACAGGAGATACTTTGTGTGATTTAAAAAGAAAAATATTATTGGAAAATATAGATTTTCCT
AAAGCTAATATGACTATGGCAGCATTACCTAAATCAAAAAATGATGAAGATAAAATGTTTTATGCCCTAGACAAATTGGT
GGAGGAAGATCCTACAATATATATAAGTAGAGATTTAGAAAATGCAGAAACATTAATATCAGGTATGGGAGATATACATT
TAGAAGTAATAGCAAATAGAGTGAAAAATAAATTTGGGACAGAAATATTATTGGATTCACCTAAAATTCCATATAGGGAG
ACTATAAAATCTATATCTAAGGTTCAAGGAAAGCATAAAAAGCAATCTGGCGGACATGGTCAATATGGAGATGTATTTAT
AAAATTCGAACCAAATAAAAATAGCGAGTTTGAATTTGTGAATAATATAGTTGGAGGGGTAGTTCCTAAACAATATATTC
CTGCAGTAGAAGAAGGAATTTTAGAATGCATGAAGAAGGGGGTCTTAGGAGAATATCCTGTTATAGGAATTAAGGCTACA
ATATACGATGGTTCATATCATTCAGTAGATTCATCTGAAATGGCTTTTAAAATTGCAGCATCTATTGCATTTAAAAAAGG
AATAAAGGAAGCAAAGCCAACCTTATTAGAACCTATAATGCGTTTAGAAGTGATATCATCAGAGGAATATATGGGGGATA
TTATAGGAGATATAAATAGGAAAAGAGGTAAAATTCTAGGTATGGAGCAGGAAGCTAATAATTTAGAAAGAATTGTAGCA
GAAGTACCTCAGGCGGAAATCCTAAATTATTCTACAGATTTAAGGAGTATAACTCAAGGTATGGGAGAATTTTCTTTAAG
CTTTGAAAGATATGATGAAATTTTAGATCCTATTGAGGTCGAAAAAGTATTATCTAGTAAAAATAAAATATAG

Upstream 100 bases:

>100_bases
GTATAGTCTGATTATTAAGAAAATTAGTAATTTTTGAAAGGATTTTATATTATCAAATAGAATATATATTTAAGCCATAA
AATAGATAGGGGGAATTAGT

Downstream 100 bases:

>100_bases
TATAAAAATCACCTAGAAACTAGGTGATTTTTATACTATATTTTATTTAGTGTTTTTTATTATTTTAGTATTTCCATTTA
GATCAAATACCTTTTGAAAT

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 690; Mature: 690

Protein sequence:

>690_residues
MNYSTTNLRNVGIIGHSGSGKTSLTEAILYFTSMVDRLGTIEEGNTVSDYDTEEKKRKISISTTIQPCKWKDTKINLIDM
PGYFDFVGEKIQGLKAADIALIVLSATGNIHVGTEKSWNYTTKEKIPKAFYINKLDKENMDFEGTLNKLKERYGTSVVPV
QYPIRKEENFKGVINVISNKATIFNDKTHKIEIKEVPNELLDEVNECKNMIVEAVAETDEKLLEKYFDEGTLSEKDIYEG
LMAGTVKGEIVPVMCGSAIKAIGIETFLEDIVRWFPSPAETKKMEVSDGINKNNIEINCNEKEPFSALVFKTIIDPFIGR
LSLFKVTSGVLNEESSIYNSTKDKKEKVGKLYFLRGKQQIQTDRVISGDIGAVSKLQYTFTGDTLCDLKRKILLENIDFP
KANMTMAALPKSKNDEDKMFYALDKLVEEDPTIYISRDLENAETLISGMGDIHLEVIANRVKNKFGTEILLDSPKIPYRE
TIKSISKVQGKHKKQSGGHGQYGDVFIKFEPNKNSEFEFVNNIVGGVVPKQYIPAVEEGILECMKKGVLGEYPVIGIKAT
IYDGSYHSVDSSEMAFKIAASIAFKKGIKEAKPTLLEPIMRLEVISSEEYMGDIIGDINRKRGKILGMEQEANNLERIVA
EVPQAEILNYSTDLRSITQGMGEFSLSFERYDEILDPIEVEKVLSSKNKI

Sequences:

>Translated_690_residues
MNYSTTNLRNVGIIGHSGSGKTSLTEAILYFTSMVDRLGTIEEGNTVSDYDTEEKKRKISISTTIQPCKWKDTKINLIDM
PGYFDFVGEKIQGLKAADIALIVLSATGNIHVGTEKSWNYTTKEKIPKAFYINKLDKENMDFEGTLNKLKERYGTSVVPV
QYPIRKEENFKGVINVISNKATIFNDKTHKIEIKEVPNELLDEVNECKNMIVEAVAETDEKLLEKYFDEGTLSEKDIYEG
LMAGTVKGEIVPVMCGSAIKAIGIETFLEDIVRWFPSPAETKKMEVSDGINKNNIEINCNEKEPFSALVFKTIIDPFIGR
LSLFKVTSGVLNEESSIYNSTKDKKEKVGKLYFLRGKQQIQTDRVISGDIGAVSKLQYTFTGDTLCDLKRKILLENIDFP
KANMTMAALPKSKNDEDKMFYALDKLVEEDPTIYISRDLENAETLISGMGDIHLEVIANRVKNKFGTEILLDSPKIPYRE
TIKSISKVQGKHKKQSGGHGQYGDVFIKFEPNKNSEFEFVNNIVGGVVPKQYIPAVEEGILECMKKGVLGEYPVIGIKAT
IYDGSYHSVDSSEMAFKIAASIAFKKGIKEAKPTLLEPIMRLEVISSEEYMGDIIGDINRKRGKILGMEQEANNLERIVA
EVPQAEILNYSTDLRSITQGMGEFSLSFERYDEILDPIEVEKVLSSKNKI
>Mature_690_residues
MNYSTTNLRNVGIIGHSGSGKTSLTEAILYFTSMVDRLGTIEEGNTVSDYDTEEKKRKISISTTIQPCKWKDTKINLIDM
PGYFDFVGEKIQGLKAADIALIVLSATGNIHVGTEKSWNYTTKEKIPKAFYINKLDKENMDFEGTLNKLKERYGTSVVPV
QYPIRKEENFKGVINVISNKATIFNDKTHKIEIKEVPNELLDEVNECKNMIVEAVAETDEKLLEKYFDEGTLSEKDIYEG
LMAGTVKGEIVPVMCGSAIKAIGIETFLEDIVRWFPSPAETKKMEVSDGINKNNIEINCNEKEPFSALVFKTIIDPFIGR
LSLFKVTSGVLNEESSIYNSTKDKKEKVGKLYFLRGKQQIQTDRVISGDIGAVSKLQYTFTGDTLCDLKRKILLENIDFP
KANMTMAALPKSKNDEDKMFYALDKLVEEDPTIYISRDLENAETLISGMGDIHLEVIANRVKNKFGTEILLDSPKIPYRE
TIKSISKVQGKHKKQSGGHGQYGDVFIKFEPNKNSEFEFVNNIVGGVVPKQYIPAVEEGILECMKKGVLGEYPVIGIKAT
IYDGSYHSVDSSEMAFKIAASIAFKKGIKEAKPTLLEPIMRLEVISSEEYMGDIIGDINRKRGKILGMEQEANNLERIVA
EVPQAEILNYSTDLRSITQGMGEFSLSFERYDEILDPIEVEKVLSSKNKI

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=682, Percent_Identity=32.9912023460411, Blast_Score=391, Evalue=1e-109,
Organism=Homo sapiens, GI19923640, Length=730, Percent_Identity=27.2602739726027, Blast_Score=302, Evalue=8e-82,
Organism=Homo sapiens, GI25306283, Length=397, Percent_Identity=28.9672544080605, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI25306287, Length=292, Percent_Identity=30.8219178082192, Blast_Score=152, Evalue=9e-37,
Organism=Escherichia coli, GI1789738, Length=680, Percent_Identity=39.5588235294118, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI1790835, Length=494, Percent_Identity=24.2914979757085, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI48994988, Length=134, Percent_Identity=32.8358208955224, Blast_Score=66, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17533571, Length=677, Percent_Identity=29.6898079763663, Blast_Score=344, Evalue=8e-95,
Organism=Caenorhabditis elegans, GI17556745, Length=708, Percent_Identity=24.0112994350282, Blast_Score=214, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6323098, Length=677, Percent_Identity=31.6100443131462, Blast_Score=345, Evalue=2e-95,
Organism=Saccharomyces cerevisiae, GI6322359, Length=773, Percent_Identity=27.0375161707633, Blast_Score=280, Evalue=4e-76,
Organism=Saccharomyces cerevisiae, GI6324707, Length=833, Percent_Identity=22.3289315726291, Blast_Score=114, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6320593, Length=833, Percent_Identity=22.3289315726291, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24582462, Length=685, Percent_Identity=33.1386861313869, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI221458488, Length=711, Percent_Identity=27.5668073136428, Blast_Score=252, Evalue=7e-67,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 77420; Mature: 77420

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYSTTNLRNVGIIGHSGSGKTSLTEAILYFTSMVDRLGTIEEGNTVSDYDTEEKKRKIS
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHEEE
ISTTIQPCKWKDTKINLIDMPGYFDFVGEKIQGLKAADIALIVLSATGNIHVGTEKSWNY
EEECCCCCCCCCCEEEEEECCCHHHHHHHHHCCCEECCEEEEEEECCCCEEECCCCCCCC
TTKEKIPKAFYINKLDKENMDFEGTLNKLKERYGTSVVPVQYPIRKEENFKGVINVISNK
CHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHCCC
ATIFNDKTHKIEIKEVPNELLDEVNECKNMIVEAVAETDEKLLEKYFDEGTLSEKDIYEG
EEEECCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LMAGTVKGEIVPVMCGSAIKAIGIETFLEDIVRWFPSPAETKKMEVSDGINKNNIEINCN
HHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHCCCCCCCEEEECC
EKEPFSALVFKTIIDPFIGRLSLFKVTSGVLNEESSIYNSTKDKKEKVGKLYFLRGKQQI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHCCEEEECCCHHH
QTDRVISGDIGAVSKLQYTFTGDTLCDLKRKILLENIDFPKANMTMAALPKSKNDEDKMF
HHCCEECCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHH
YALDKLVEEDPTIYISRDLENAETLISGMGDIHLEVIANRVKNKFGTEILLDSPKIPYRE
HHHHHHHCCCCCEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCCCCHHH
TIKSISKVQGKHKKQSGGHGQYGDVFIKFEPNKNSEFEFVNNIVGGVVPKQYIPAVEEGI
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCHHHCCHHHHHH
LECMKKGVLGEYPVIGIKATIYDGSYHSVDSSEMAFKIAASIAFKKGIKEAKPTLLEPIM
HHHHHHCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RLEVISSEEYMGDIIGDINRKRGKILGMEQEANNLERIVAEVPQAEILNYSTDLRSITQG
HHHHHCCHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHC
MGEFSLSFERYDEILDPIEVEKVLSSKNKI
HHHHEECHHHHHHHHCHHHHHHHHHCCCCC
>Mature Secondary Structure
MNYSTTNLRNVGIIGHSGSGKTSLTEAILYFTSMVDRLGTIEEGNTVSDYDTEEKKRKIS
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHEEE
ISTTIQPCKWKDTKINLIDMPGYFDFVGEKIQGLKAADIALIVLSATGNIHVGTEKSWNY
EEECCCCCCCCCCEEEEEECCCHHHHHHHHHCCCEECCEEEEEEECCCCEEECCCCCCCC
TTKEKIPKAFYINKLDKENMDFEGTLNKLKERYGTSVVPVQYPIRKEENFKGVINVISNK
CHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHCCC
ATIFNDKTHKIEIKEVPNELLDEVNECKNMIVEAVAETDEKLLEKYFDEGTLSEKDIYEG
EEEECCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LMAGTVKGEIVPVMCGSAIKAIGIETFLEDIVRWFPSPAETKKMEVSDGINKNNIEINCN
HHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHCCCCCCCEEEECC
EKEPFSALVFKTIIDPFIGRLSLFKVTSGVLNEESSIYNSTKDKKEKVGKLYFLRGKQQI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHCCEEEECCCHHH
QTDRVISGDIGAVSKLQYTFTGDTLCDLKRKILLENIDFPKANMTMAALPKSKNDEDKMF
HHCCEECCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHH
YALDKLVEEDPTIYISRDLENAETLISGMGDIHLEVIANRVKNKFGTEILLDSPKIPYRE
HHHHHHHCCCCCEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCCCCHHH
TIKSISKVQGKHKKQSGGHGQYGDVFIKFEPNKNSEFEFVNNIVGGVVPKQYIPAVEEGI
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCHHHCCHHHHHH
LECMKKGVLGEYPVIGIKATIYDGSYHSVDSSEMAFKIAASIAFKKGIKEAKPTLLEPIM
HHHHHHCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RLEVISSEEYMGDIIGDINRKRGKILGMEQEANNLERIVAEVPQAEILNYSTDLRSITQG
HHHHHCCHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHC
MGEFSLSFERYDEILDPIEVEKVLSSKNKI
HHHHEECHHHHHHHHCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA