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The map label for this gene is proS [H]

Identifier: 226950957

GI number: 226950957

Start: 3999255

End: 4000892

Strand: Reverse

Name: proS [H]

Synonym: CLM_3974

Alternate gene names: 226950957

Gene position: 4000892-3999255 (Counterclockwise)

Preceding gene: 226950958

Following gene: 226950956

Centisome position: 96.28

GC content: 26.8

Gene sequence:

>1638_bases
ATGAAGTTATCAAAAACATTAGTACATAGTTTAAGAGAGGATCCAGCAGATGTGGAGATGGATAGTCAAAAATTATTATT
AAAAGGAGGATTAATAGCTAAAGTAGATAGTGGACTATATGCATTTACACCTTTAGGATATAGGTTCTTAGAAGATATTC
AAAAGGTAGTTGAAAATAATTCAAAAAAAATTGGCGGTTCCCAAATATCTATACCTTATGTAAACAATTTGGAAGAGGTA
AGTGAAAAAAATAATGGATTTATATTAAATTCTAATGAGGAGACCTTAAGCTTTATAAACTTTTTAAAAAGCAGTGTAAC
ATCCTATAAACAATTACCTTTGTTTTTTTATGAAAACAATACAGAGTTTAGCTATAAAGGTAAAAATAATTTAGGAATTA
TGGGTGGAAGACAAATATTAAGAGAGTATTTTTATATGGTGTTAGATGGAGAAAATGATAAATTTAATAAAAAAGAATTA
ATAAAACTATATAAAAATATCTTTGAAATCATGAATTTACAATACAATATGGTAGAGGATGAATTAGATGGAACCATAAA
ATTTATCATATATAATAATATAGGAGATTTACGTATAGTCGCGTGTAAAAGCTGTAATTATGGTAATGAAGTCAATAGAG
CTTCATCTATACCAGATTATCAGATAGAAAAGGATTTAAAAGAATTAAATAAAATAGAAACACTTGATATAAAAACTATA
GAGGAGCTAGGTGAATTTTTTAAGGTGCCTTATAGAAAGTTTACAAAAACAATAATATATAAGTGCAATAATGATTCTAT
TGTTGCAGTTATGGTAAGAGGCGATAGAGATATAGATGAATTTAAGGTTATTAAAAATTTAGGTAATATAAAGAGTTTAG
AATTAGCAGGAGAAGATATAGTTAGACAGGCTACAAATGCAGAAGTAGGTTTTGCTGGTCCAATTAATTTAAAAGTTGAT
AAGATATTGATAGATGAAGAAATAACTAAAATGAATAATTTCATGGTAGGTGCAAATGAAACAGGTTATCATTATGAAAA
TGTTAACTATCAAAGAGATTTTAAGGGAATAGTTGGGGAATTTAGAAAGATACATAAAGATAGCAAATGTACTCTATGTG
GTGATAAATTAGAGGTTAATGAAGGGTTTGTTATAGGGGAAATCAAAAAAATAGAAGAAAACTTAATAAAAAATGAATGT
GCTACATTCATTGATAATAAGGGAAAGAGTAAACCGTTCACAATTTATAAAGGTTTTATGGATATATATAAAATTATTTC
CTGGACAATAGAACAAAATAAAGATGAATTAGGGATTGTTTGGCCTATGGAGACATCAGCATTTAAAGTTATAGTAACTA
TAGCTAATGTAAAGGATGAACAACAGTTTAAAGTAGGAGAAAAAATTTATAGTAGTTTAGTTAATATTGGAATAGGTACA
ATTTTAGATGATAGAAAAGAAAGAGCAGGAGTAAAATTTAAAGATGCGGATTTATGGGGAATACCTATTAGAATAACCGT
TGGTAAAAACATAAAAGAAAATAATGTAGAAATAAAATTAAGGAATTCAAAGGAGAAGAAAGAAATCCCAATAGATCAAT
TACAAGAAAGTATTAAAAGTTTATTGGGTATAGGTTAA

Upstream 100 bases:

>100_bases
CGTCCTAATAAGGGGCGAGTTTTGTTTATATATTGAATGTTTAAAATTTCTATCGAGAATATTTTTAAATATAATGAATA
GTTTGTTAGGAGGTTAAGTT

Downstream 100 bases:

>100_bases
TAGATTGAATAAAAATATTATATGGTTTATTATAAAAATATAATTTAGAATGGGATGGGAGATAGATGATTATGGAGTTA
AACTTAGATACAATAAAAAA

Product: putative prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MKLSKTLVHSLREDPADVEMDSQKLLLKGGLIAKVDSGLYAFTPLGYRFLEDIQKVVENNSKKIGGSQISIPYVNNLEEV
SEKNNGFILNSNEETLSFINFLKSSVTSYKQLPLFFYENNTEFSYKGKNNLGIMGGRQILREYFYMVLDGENDKFNKKEL
IKLYKNIFEIMNLQYNMVEDELDGTIKFIIYNNIGDLRIVACKSCNYGNEVNRASSIPDYQIEKDLKELNKIETLDIKTI
EELGEFFKVPYRKFTKTIIYKCNNDSIVAVMVRGDRDIDEFKVIKNLGNIKSLELAGEDIVRQATNAEVGFAGPINLKVD
KILIDEEITKMNNFMVGANETGYHYENVNYQRDFKGIVGEFRKIHKDSKCTLCGDKLEVNEGFVIGEIKKIEENLIKNEC
ATFIDNKGKSKPFTIYKGFMDIYKIISWTIEQNKDELGIVWPMETSAFKVIVTIANVKDEQQFKVGEKIYSSLVNIGIGT
ILDDRKERAGVKFKDADLWGIPIRITVGKNIKENNVEIKLRNSKEKKEIPIDQLQESIKSLLGIG

Sequences:

>Translated_545_residues
MKLSKTLVHSLREDPADVEMDSQKLLLKGGLIAKVDSGLYAFTPLGYRFLEDIQKVVENNSKKIGGSQISIPYVNNLEEV
SEKNNGFILNSNEETLSFINFLKSSVTSYKQLPLFFYENNTEFSYKGKNNLGIMGGRQILREYFYMVLDGENDKFNKKEL
IKLYKNIFEIMNLQYNMVEDELDGTIKFIIYNNIGDLRIVACKSCNYGNEVNRASSIPDYQIEKDLKELNKIETLDIKTI
EELGEFFKVPYRKFTKTIIYKCNNDSIVAVMVRGDRDIDEFKVIKNLGNIKSLELAGEDIVRQATNAEVGFAGPINLKVD
KILIDEEITKMNNFMVGANETGYHYENVNYQRDFKGIVGEFRKIHKDSKCTLCGDKLEVNEGFVIGEIKKIEENLIKNEC
ATFIDNKGKSKPFTIYKGFMDIYKIISWTIEQNKDELGIVWPMETSAFKVIVTIANVKDEQQFKVGEKIYSSLVNIGIGT
ILDDRKERAGVKFKDADLWGIPIRITVGKNIKENNVEIKLRNSKEKKEIPIDQLQESIKSLLGIG
>Mature_545_residues
MKLSKTLVHSLREDPADVEMDSQKLLLKGGLIAKVDSGLYAFTPLGYRFLEDIQKVVENNSKKIGGSQISIPYVNNLEEV
SEKNNGFILNSNEETLSFINFLKSSVTSYKQLPLFFYENNTEFSYKGKNNLGIMGGRQILREYFYMVLDGENDKFNKKEL
IKLYKNIFEIMNLQYNMVEDELDGTIKFIIYNNIGDLRIVACKSCNYGNEVNRASSIPDYQIEKDLKELNKIETLDIKTI
EELGEFFKVPYRKFTKTIIYKCNNDSIVAVMVRGDRDIDEFKVIKNLGNIKSLELAGEDIVRQATNAEVGFAGPINLKVD
KILIDEEITKMNNFMVGANETGYHYENVNYQRDFKGIVGEFRKIHKDSKCTLCGDKLEVNEGFVIGEIKKIEENLIKNEC
ATFIDNKGKSKPFTIYKGFMDIYKIISWTIEQNKDELGIVWPMETSAFKVIVTIANVKDEQQFKVGEKIYSSLVNIGIGT
ILDDRKERAGVKFKDADLWGIPIRITVGKNIKENNVEIKLRNSKEKKEIPIDQLQESIKSLLGIG

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786392, Length=561, Percent_Identity=29.0552584670232, Blast_Score=242, Evalue=4e-65,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 62323; Mature: 62323

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSKTLVHSLREDPADVEMDSQKLLLKGGLIAKVDSGLYAFTPLGYRFLEDIQKVVENN
CCCHHHHHHHHHCCCCCCCCCCCCEEECCCEEEEECCCEEEECCCHHHHHHHHHHHHHCC
SKKIGGSQISIPYVNNLEEVSEKNNGFILNSNEETLSFINFLKSSVTSYKQLPLFFYENN
CCCCCCCEEECCCCCCHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCEEEEEECC
TEFSYKGKNNLGIMGGRQILREYFYMVLDGENDKFNKKELIKLYKNIFEIMNLQYNMVED
CEEEECCCCCCEECCHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
ELDGTIKFIIYNNIGDLRIVACKSCNYGNEVNRASSIPDYQIEKDLKELNKIETLDIKTI
CCCCEEEEEEEECCCCEEEEEEECCCCCCCCCHHCCCCCHHHHHHHHHHHCEEECCHHHH
EELGEFFKVPYRKFTKTIIYKCNNDSIVAVMVRGDRDIDEFKVIKNLGNIKSLELAGEDI
HHHHHHHHCCHHHHCEEEEEEECCCCEEEEEEECCCCHHHHHHHHHCCCCEEEEECCHHH
VRQATNAEVGFAGPINLKVDKILIDEEITKMNNFMVGANETGYHYENVNYQRDFKGIVGE
HHHHCCCCCCCCCCCCEEEEEEEECHHHHHHHCEEEECCCCCCEECCCCCHHHHHHHHHH
FRKIHKDSKCTLCGDKLEVNEGFVIGEIKKIEENLIKNECATFIDNKGKSKPFTIYKGFM
HHHHCCCCCCEEECCEEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHH
DIYKIISWTIEQNKDELGIVWPMETSAFKVIVTIANVKDEQQFKVGEKIYSSLVNIGIGT
HHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCHHH
ILDDRKERAGVKFKDADLWGIPIRITVGKNIKENNVEIKLRNSKEKKEIPIDQLQESIKS
HCCCHHHHCCCEEECCCEECEEEEEEECCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHH
LLGIG
HHCCC
>Mature Secondary Structure
MKLSKTLVHSLREDPADVEMDSQKLLLKGGLIAKVDSGLYAFTPLGYRFLEDIQKVVENN
CCCHHHHHHHHHCCCCCCCCCCCCEEECCCEEEEECCCEEEECCCHHHHHHHHHHHHHCC
SKKIGGSQISIPYVNNLEEVSEKNNGFILNSNEETLSFINFLKSSVTSYKQLPLFFYENN
CCCCCCCEEECCCCCCHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCEEEEEECC
TEFSYKGKNNLGIMGGRQILREYFYMVLDGENDKFNKKELIKLYKNIFEIMNLQYNMVED
CEEEECCCCCCEECCHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
ELDGTIKFIIYNNIGDLRIVACKSCNYGNEVNRASSIPDYQIEKDLKELNKIETLDIKTI
CCCCEEEEEEEECCCCEEEEEEECCCCCCCCCHHCCCCCHHHHHHHHHHHCEEECCHHHH
EELGEFFKVPYRKFTKTIIYKCNNDSIVAVMVRGDRDIDEFKVIKNLGNIKSLELAGEDI
HHHHHHHHCCHHHHCEEEEEEECCCCEEEEEEECCCCHHHHHHHHHCCCCEEEEECCHHH
VRQATNAEVGFAGPINLKVDKILIDEEITKMNNFMVGANETGYHYENVNYQRDFKGIVGE
HHHHCCCCCCCCCCCCEEEEEEEECHHHHHHHCEEEECCCCCCEECCCCCHHHHHHHHHH
FRKIHKDSKCTLCGDKLEVNEGFVIGEIKKIEENLIKNECATFIDNKGKSKPFTIYKGFM
HHHHCCCCCCEEECCEEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHH
DIYKIISWTIEQNKDELGIVWPMETSAFKVIVTIANVKDEQQFKVGEKIYSSLVNIGIGT
HHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCHHH
ILDDRKERAGVKFKDADLWGIPIRITVGKNIKENNVEIKLRNSKEKKEIPIDQLQESIKS
HCCCHHHHCCCEEECCCEECEEEEEEECCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHH
LLGIG
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]