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The map label for this gene is fldI [H]

Identifier: 226950716

GI number: 226950716

Start: 3739874

End: 3740668

Strand: Reverse

Name: fldI [H]

Synonym: CLM_3724

Alternate gene names: 226950716

Gene position: 3740668-3739874 (Counterclockwise)

Preceding gene: 226950717

Following gene: 226950715

Centisome position: 90.02

GC content: 33.58

Gene sequence:

>795_bases
ATGGCAGATATTTATACTATGGGTGTAGATATAGGTTCAACTGCATCAAAAACAGTAGTATTAAAAAATGGAAAAGAAAT
TGTAAGTCAGGCAGTAATAAATGTAGGGGCCGGAACAAGCGGCCCCAAAAGAGCTATAGATTCTGTATTAAGAGAAGCAA
AATTATCTATTGAAGATTTAGATTATATTGTATCTACTGGGTATGGAAGAAATAGTTTTGAGTTTGCCAACAAACAAATT
TCTGAATTAAGTTGTCATGCAAAGGGAGTCTATTTTGATAACAATAAAGCTAGAACAGTTATTGATATAGGCGGTCAAGA
TATTAAAGTATTAAAATTAGCGGATAGTGGAAGACTTTTAAATTTCATAATGAATGATAAATGTGCTGCAGGAACAGGAC
GATTTTTAGATGTAATGTCTAGAGTAATAGAAATTCCAGTTGATGAGCTAGGAAAAAAAGCATTAGAAAGCAAAAATCCT
TGTACTATTAGTTCTACCTGTACAGTATTTGCAGAGTCAGAAGTAATTTCTCAACTTGCAAGAGGAGTTAAAACTGAAGA
TTTGATAGCAGGAATTTGTAAGTCTGTAGCATCAAGAGTGGCTAGCCTTGCAAAGAGAAGTGGTATAGAAGAATTAGTAG
TTATGAGTGGAGGAGTAGCTAAAAATATAGGTGTAGTAAAGGCAATGGAAGCAGAATTGGGAAAAGACATTTATATATCT
GAAAATTCCCAATTGAATGGAGCATTGGGAGCAAGTCTATACGCTTATGAAAGTTTTCAAAAAGAAAGGAGCTAA

Upstream 100 bases:

>100_bases
CAGAAAATACTGCAGAAGTTTTAAAAGAAATGGGATACACAGAAGAAGATATTCAAGAATTAGAAAAAAATAAAGATATT
ATGGTAAGGAAGGAAAAATA

Downstream 100 bases:

>100_bases
AAACATGAGTGATAGAAATAAGGAAGTAAAAGAAAAAAAAGCAAAGCATTATCTTAGAGAGATTACTGCAAAGCATTATA
AAGAGGCTCTCGAAGCAAAA

Product: R-phenyllactate dehydratase activator

Products: NA

Alternate protein names: 2-hydroxyglutaryl-CoA dehydratase component A [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MADIYTMGVDIGSTASKTVVLKNGKEIVSQAVINVGAGTSGPKRAIDSVLREAKLSIEDLDYIVSTGYGRNSFEFANKQI
SELSCHAKGVYFDNNKARTVIDIGGQDIKVLKLADSGRLLNFIMNDKCAAGTGRFLDVMSRVIEIPVDELGKKALESKNP
CTISSTCTVFAESEVISQLARGVKTEDLIAGICKSVASRVASLAKRSGIEELVVMSGGVAKNIGVVKAMEAELGKDIYIS
ENSQLNGALGASLYAYESFQKERS

Sequences:

>Translated_264_residues
MADIYTMGVDIGSTASKTVVLKNGKEIVSQAVINVGAGTSGPKRAIDSVLREAKLSIEDLDYIVSTGYGRNSFEFANKQI
SELSCHAKGVYFDNNKARTVIDIGGQDIKVLKLADSGRLLNFIMNDKCAAGTGRFLDVMSRVIEIPVDELGKKALESKNP
CTISSTCTVFAESEVISQLARGVKTEDLIAGICKSVASRVASLAKRSGIEELVVMSGGVAKNIGVVKAMEAELGKDIYIS
ENSQLNGALGASLYAYESFQKERS
>Mature_263_residues
ADIYTMGVDIGSTASKTVVLKNGKEIVSQAVINVGAGTSGPKRAIDSVLREAKLSIEDLDYIVSTGYGRNSFEFANKQIS
ELSCHAKGVYFDNNKARTVIDIGGQDIKVLKLADSGRLLNFIMNDKCAAGTGRFLDVMSRVIEIPVDELGKKALESKNPC
TISSTCTVFAESEVISQLARGVKTEDLIAGICKSVASRVASLAKRSGIEELVVMSGGVAKNIGVVKAMEAELGKDIYISE
NSQLNGALGASLYAYESFQKERS

Specific function: Required for the activation of (R)-2-hydroxyglutaryl-CoA dehydratase. This protein is extremely sensitive towards oxygen [H]

COG id: COG1924

COG function: function code I; Activator of 2-hydroxyglutaryl-CoA dehydratase (HSP70-class ATPase domain)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli yjiL and M.jannaschii MJ0004 and MJ0800 [H]

Homologues:

Organism=Escherichia coli, GI87082426, Length=249, Percent_Identity=39.3574297188755, Blast_Score=181, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002731
- InterPro:   IPR008275 [H]

Pfam domain/function: PF01869 BcrAD_BadFG [H]

EC number: NA

Molecular weight: Translated: 28147; Mature: 28015

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADIYTMGVDIGSTASKTVVLKNGKEIVSQAVINVGAGTSGPKRAIDSVLREAKLSIEDL
CCCEEEECCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHH
DYIVSTGYGRNSFEFANKQISELSCHAKGVYFDNNKARTVIDIGGQDIKVLKLADSGRLL
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCEEEEEEECCCCEE
NFIMNDKCAAGTGRFLDVMSRVIEIPVDELGKKALESKNPCTISSTCTVFAESEVISQLA
HHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCEEECCCEEEEEHHHHHHHHH
RGVKTEDLIAGICKSVASRVASLAKRSGIEELVVMSGGVAKNIGVVKAMEAELGKDIYIS
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHCCHHHHHHHHHCCCEEEEE
ENSQLNGALGASLYAYESFQKERS
CCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADIYTMGVDIGSTASKTVVLKNGKEIVSQAVINVGAGTSGPKRAIDSVLREAKLSIEDL
CCEEEECCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHH
DYIVSTGYGRNSFEFANKQISELSCHAKGVYFDNNKARTVIDIGGQDIKVLKLADSGRLL
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCEEEEEEECCCCEE
NFIMNDKCAAGTGRFLDVMSRVIEIPVDELGKKALESKNPCTISSTCTVFAESEVISQLA
HHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCEEECCCEEEEEHHHHHHHHH
RGVKTEDLIAGICKSVASRVASLAKRSGIEELVVMSGGVAKNIGVVKAMEAELGKDIYIS
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHCCHHHHHHHHHCCCEEEEE
ENSQLNGALGASLYAYESFQKERS
CCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8365476; 2659350; 7607244; 11106419; 11243821 [H]