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The map label for this gene is ldhA [C]

Identifier: 226950709

GI number: 226950709

Start: 3730996

End: 3731991

Strand: Reverse

Name: ldhA [C]

Synonym: CLM_3717

Alternate gene names: 226950709

Gene position: 3731991-3730996 (Counterclockwise)

Preceding gene: 226950710

Following gene: 226950706

Centisome position: 89.81

GC content: 30.92

Gene sequence:

>996_bases
ATGAAAATCTTAGCTTATTGTGTAAGACCTGATGAAGTTGATTCATTCAAAAAATTTTCTGAAAAATATGGACATACTGT
TGATTTAATACCAGATTCTTTTGGTCCCAATGTGGCTCATTTAGCAAAAGGATATGATGGTATCTCAATTTTAGGAAATG
ATACTTGTAACAGAGAGGCACTAGAAAAGATTAAAGATTGTGGTATAAAATACCTAGCAACTAGAACTGCTGGAGTAAAT
AATATAGATTTTGATGCTGCAAAAGAATTTGGCATAAATGTAGCTAATGTTCCAGCTTATTCTCCTAATTCAGTTTCAGA
GTTTACTATAGGACTTGCATTATCCCTAACAAGAAAAATACCTTTTGCATTAAAAAGAGTAGAACTTAATAATTTTGCAC
TAGGTGGATTAATAGGGGTAGAATTAAGAAACTTAACTCTAGGAGTTATAGGAACTGGAAGAATAGGTTTAAAAGTAATT
GAAGGCTTTTCAGGATTTGGTATGAAAAAGATGATAGGTTACGATCTTTTTGAAAATGAAGAAGCTAAAAAATATATTGA
ATATAAATCCTTAGATGAAGTATTCAAAGAAGCAGATATAATAACACTACATGCACCTTTGACAGACGATAATTACCATA
TGATAGGAAAAGAAAGTATTGCTAAAATGAAAGATGGTGTATTCATAATAAATGCAGCACGTGGAGCATTAATTGATAGT
GAAGCTTTAATAGAAGGGCTAAAATCTGGTAAAATAGCTGGAGCAGCCTTAGATTCTTATGAATATGAACAAGGAGTCTT
CCATAATAATAAAATGAACGAAATCATGCAAGACGATACATTAGAAAGACTTAAGAGTTTCCCAAATGTAGTTATTACAC
CTCATTTAGGATTCTATACAGATGAAGCCGTATCAAATATGGTTGAAATTACTCTTATGAATTTGCAAGAATTTGAATTA
AAAGGAACTTGTAAAAATCAGAGGGTATGTAAGTAA

Upstream 100 bases:

>100_bases
ATGTAATATATCATTTTTATTGACAAATATTAAACAATATGATAGGCTGAATTTGATAGAAAATTATGAAAAAGAAAACT
TACAATGGGGGGTATAAATT

Downstream 100 bases:

>100_bases
TTTTAAGTGTTTTTAGTATGGAAATGAATTGTTAAAAATAAATTTGTAAAGAAAATATATTTAGATTCAATTTTAAAATT
TAAGAAAATAATAAAATGGT

Product: D-lactate dehydrogenase

Products: NA

Alternate protein names: D-LDH; D-specific D-2-hydroxyacid dehydrogenase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKILAYCVRPDEVDSFKKFSEKYGHTVDLIPDSFGPNVAHLAKGYDGISILGNDTCNREALEKIKDCGIKYLATRTAGVN
NIDFDAAKEFGINVANVPAYSPNSVSEFTIGLALSLTRKIPFALKRVELNNFALGGLIGVELRNLTLGVIGTGRIGLKVI
EGFSGFGMKKMIGYDLFENEEAKKYIEYKSLDEVFKEADIITLHAPLTDDNYHMIGKESIAKMKDGVFIINAARGALIDS
EALIEGLKSGKIAGAALDSYEYEQGVFHNNKMNEIMQDDTLERLKSFPNVVITPHLGFYTDEAVSNMVEITLMNLQEFEL
KGTCKNQRVCK

Sequences:

>Translated_331_residues
MKILAYCVRPDEVDSFKKFSEKYGHTVDLIPDSFGPNVAHLAKGYDGISILGNDTCNREALEKIKDCGIKYLATRTAGVN
NIDFDAAKEFGINVANVPAYSPNSVSEFTIGLALSLTRKIPFALKRVELNNFALGGLIGVELRNLTLGVIGTGRIGLKVI
EGFSGFGMKKMIGYDLFENEEAKKYIEYKSLDEVFKEADIITLHAPLTDDNYHMIGKESIAKMKDGVFIINAARGALIDS
EALIEGLKSGKIAGAALDSYEYEQGVFHNNKMNEIMQDDTLERLKSFPNVVITPHLGFYTDEAVSNMVEITLMNLQEFEL
KGTCKNQRVCK
>Mature_331_residues
MKILAYCVRPDEVDSFKKFSEKYGHTVDLIPDSFGPNVAHLAKGYDGISILGNDTCNREALEKIKDCGIKYLATRTAGVN
NIDFDAAKEFGINVANVPAYSPNSVSEFTIGLALSLTRKIPFALKRVELNNFALGGLIGVELRNLTLGVIGTGRIGLKVI
EGFSGFGMKKMIGYDLFENEEAKKYIEYKSLDEVFKEADIITLHAPLTDDNYHMIGKESIAKMKDGVFIINAARGALIDS
EALIEGLKSGKIAGAALDSYEYEQGVFHNNKMNEIMQDDTLERLKSFPNVVITPHLGFYTDEAVSNMVEITLMNLQEFEL
KGTCKNQRVCK

Specific function: Fermentative Lactate Dehydrogenase. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=234, Percent_Identity=35.4700854700855, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI61743967, Length=268, Percent_Identity=32.4626865671642, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI4557497, Length=268, Percent_Identity=32.4626865671642, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI145580578, Length=268, Percent_Identity=32.8358208955224, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI4557499, Length=268, Percent_Identity=32.8358208955224, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI145580575, Length=276, Percent_Identity=31.8840579710145, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI6912396, Length=277, Percent_Identity=27.4368231046931, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1787645, Length=330, Percent_Identity=39.6969696969697, Blast_Score=258, Evalue=3e-70,
Organism=Escherichia coli, GI87082289, Length=322, Percent_Identity=29.5031055900621, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1789279, Length=233, Percent_Identity=33.4763948497854, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788660, Length=269, Percent_Identity=28.2527881040892, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=237, Percent_Identity=37.9746835443038, Blast_Score=144, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI25147481, Length=260, Percent_Identity=29.6153846153846, Blast_Score=103, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6320925, Length=232, Percent_Identity=34.9137931034483, Blast_Score=134, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6322116, Length=232, Percent_Identity=34.9137931034483, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6324055, Length=252, Percent_Identity=32.5396825396825, Blast_Score=132, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6324964, Length=211, Percent_Identity=28.9099526066351, Blast_Score=93, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324980, Length=137, Percent_Identity=31.3868613138686, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325144, Length=128, Percent_Identity=34.375, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19921140, Length=237, Percent_Identity=38.8185654008439, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24646446, Length=275, Percent_Identity=31.2727272727273, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24646448, Length=275, Percent_Identity=31.2727272727273, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24646452, Length=275, Percent_Identity=31.2727272727273, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24646450, Length=275, Percent_Identity=31.2727272727273, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI62472511, Length=275, Percent_Identity=31.2727272727273, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI28574286, Length=280, Percent_Identity=26.7857142857143, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45552429, Length=282, Percent_Identity=25.886524822695, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24585514, Length=282, Percent_Identity=25.886524822695, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI28574282, Length=282, Percent_Identity=25.886524822695, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI28574284, Length=282, Percent_Identity=25.886524822695, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI45551003, Length=282, Percent_Identity=25.886524822695, Blast_Score=104, Evalue=8e-23,
Organism=Drosophila melanogaster, GI28571528, Length=320, Percent_Identity=27.5, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24585516, Length=236, Percent_Identity=23.3050847457627, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.28 [H]

Molecular weight: Translated: 36590; Mature: 36590

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILAYCVRPDEVDSFKKFSEKYGHTVDLIPDSFGPNVAHLAKGYDGISILGNDTCNREA
CCEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCCCCHHHHHCCCCCEEEECCCCCCHHH
LEKIKDCGIKYLATRTAGVNNIDFDAAKEFGINVANVPAYSPNSVSEFTIGLALSLTRKI
HHHHHHCCEEEEEECCCCCCCCCCHHHHHHCCEEECCCCCCCCCCHHEEHHHHHHHHHCC
PFALKRVELNNFALGGLIGVELRNLTLGVIGTGRIGLKVIEGFSGFGMKKMIGYDLFENE
CHHEEEEECCCEEECCEEEEEEEEEEEEEEECCCEEEEEECCCCCCCHHHHHCCHHHCCC
EAKKYIEYKSLDEVFKEADIITLHAPLTDDNYHMIGKESIAKMKDGVFIINAARGALIDS
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEECHHHHHHHHCCEEEEEECCCCEECH
EALIEGLKSGKIAGAALDSYEYEQGVFHNNKMNEIMQDDTLERLKSFPNVVITPHLGFYT
HHHHHHHHCCCEEEEECCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
DEAVSNMVEITLMNLQEFELKGTCKNQRVCK
HHHHHHEEEEEEECCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKILAYCVRPDEVDSFKKFSEKYGHTVDLIPDSFGPNVAHLAKGYDGISILGNDTCNREA
CCEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCCCCHHHHHCCCCCEEEECCCCCCHHH
LEKIKDCGIKYLATRTAGVNNIDFDAAKEFGINVANVPAYSPNSVSEFTIGLALSLTRKI
HHHHHHCCEEEEEECCCCCCCCCCHHHHHHCCEEECCCCCCCCCCHHEEHHHHHHHHHCC
PFALKRVELNNFALGGLIGVELRNLTLGVIGTGRIGLKVIEGFSGFGMKKMIGYDLFENE
CHHEEEEECCCEEECCEEEEEEEEEEEEEEECCCEEEEEECCCCCCCHHHHHCCHHHCCC
EAKKYIEYKSLDEVFKEADIITLHAPLTDDNYHMIGKESIAKMKDGVFIINAARGALIDS
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEECHHHHHHHHCCEEEEEECCCCEECH
EALIEGLKSGKIAGAALDSYEYEQGVFHNNKMNEIMQDDTLERLKSFPNVVITPHLGFYT
HHHHHHHHCCCEEEEECCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
DEAVSNMVEITLMNLQEFELKGTCKNQRVCK
HHHHHHEEEEEEECCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1840590; 8740366 [H]