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The map label for this gene is ligA [H]

Identifier: 226950696

GI number: 226950696

Start: 3716539

End: 3718533

Strand: Reverse

Name: ligA [H]

Synonym: CLM_3704

Alternate gene names: 226950696

Gene position: 3718533-3716539 (Counterclockwise)

Preceding gene: 226950697

Following gene: 226950695

Centisome position: 89.49

GC content: 29.32

Gene sequence:

>1995_bases
ATGGATAACAAGTTAGAAAAGATGAAGGAACTTGTAGAAGAATTAAATCAGTATGCCTATGAGTATTATGTATTAGATAA
TCCCAGTATATCTGATAAAGAATATGACTTGAAATATGATGAATTAGTAATTTTGGAGAAGAAAACTGAAGTTACATTAC
CTTATTCACCAACCCAAAGGGTTGGTGATAAAATATTAGGTGAATTTAGTAAATATACTCACAAAGGAAGACTCTGGAGT
TTGGATAAAGCCCAAAATATGGAGCAGTTAATAGAGTGGCATAATAGAAATTTAAAAGTTATAGAGCAATATAATTCTAT
GAGTGAAGATAAATTACCAGAGTTGAGATATATAGTTACTAAAAAATTTGATGGATTAACGGTAAATTGTACCTATGATG
AAAATGGAATTTTAATAAAGAGTGCTACAAGAGGAACGGGAATTATAGGTGAAGATATAACAGCTCAAATAAAAACTATA
AAGACAGTTCCATTAAAAATAAAAAATAACCACGTTATAGAAGTGCATGGAGAAGCCATAATGACTAAAACAGCTTTTGA
AGAATATAATAAAGCTGCTCAAGTTCCACTAAAAAATTTAAGAAATGGAGCAGCAGGAGCTCTTAGAAATTTAGATATTA
AAGAAACTGCTAGAAGGAATTTATCCGCATTTTTTTATGATGTAGGTTATAATGAAGGTCCCGAATTTAAAAGTTATAGA
GAAATGATGAATTTTATAAAAAATATGGGATTGCCTCAGGATAAGTATATTAAAGAATGTACTAACATGGAAGAAGTGGA
AAAAGAAATTGAATATATAGAATCAATAAGAGGAGAACTAGATTATGATATAGATGGAGCAGTAATTGTTGTAGATGATA
TTAAAACAAGAGAGATATTAGGGTATACTATAAAATTCCCCAAATGGGCTATAGCTTATAAATTTGAAGCTAAGGAAATT
ACTACAAAGCTTCTAGATGTAGAATGGAATGTAGGAAGAAGCGGAAGAGTTACTCCAACAGCTTTATTAGAACCCGTAGA
ACTAGGCGGAGTCACAGTAAAAAGAGCTACACTAAATAATATGGATGATATTAAGAGAAAAAATGTAAAATTGGGTGCTA
AGGTTTTAGTTAGAAGATCTAACGATGTTATACCGGAGATAATGGGAGTAGTAGAAGAATCTTTAGAGGAAAGCGAGGAA
ATACAGGCACCAGATAGATGTCCTTATTGTAATAGCCATCTAGTACAAAATGGAGTACATTATTATTGTGAGAATACATT
ATCTTGTAAACCACAGATGGTAAAAAGTATAGTTCATTTTGCTAGTAGAGAAGCTATGAATATAGCAGGATTCAGTGAAA
AAACTGCAGAACAACTTTTTGAAAAATTAGATATAAAATCTATAGCAGATTTGTATAAGATAAAAAAAGAAGAACTATTA
ACTTTAGAAAAATTTAAGGATAAAAAGTCTCAAAATCTTATAGATGCTATACAAAATAGCAAAAATTGTGATTTGGCATC
CTTTATATACGCTTTAGGTATACCTAATGTAGGTAAAAAGACTGCAAATGATTTAGTAATGAAATTTAAAACTTTAGAAT
CTATAAAGAATACAACAATAGAACAATTAGTAGAAGTTCCAGATGTGGGAGAAATAGTAGCTAAGAGCATATATGATTTT
TTTGAAGACGAAAAAATTATAAGTAACATAGAAGAATTATTAAATTTGGGTGTAAAACCTTATTATGAAGAAGAAAGAAT
TGATGAGAATCCTTTTATGGGTAAAACTATAGTTGTAACAGGAAGTTTGAATAATTATAGCAGAGGGGAAATAAAAGATA
AATTACAGTCTTTAGGAGCTAAAGTTTCTAGTAGTGTAAGTAAAAATACAGACTATGTCTTAGTAGGGGAAAAACCAGGA
TCTAAATATGAAAAAGCAATTGAATTAGGAGTAAAAGTAATCAACGAAGAAGAATTTTCTAATAAAATTAAATAA

Upstream 100 bases:

>100_bases
TTTCGGATGACATAAAGTTAACTATTGCTTTTGACCGTAGAGGTGTTAAAATATTAATGCTTAGTATGGCGCCAATTGAA
GCGGTTTAGGAGGGATATAT

Downstream 100 bases:

>100_bases
TAACATTATAGAAGCATATATTTACAATGAATGAAAAAAGGTATATTATTTAATTGGTTTAATTTATAATAATAGCTCAA
AGAATTTGGAGAGGATAAAA

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 664; Mature: 664

Protein sequence:

>664_residues
MDNKLEKMKELVEELNQYAYEYYVLDNPSISDKEYDLKYDELVILEKKTEVTLPYSPTQRVGDKILGEFSKYTHKGRLWS
LDKAQNMEQLIEWHNRNLKVIEQYNSMSEDKLPELRYIVTKKFDGLTVNCTYDENGILIKSATRGTGIIGEDITAQIKTI
KTVPLKIKNNHVIEVHGEAIMTKTAFEEYNKAAQVPLKNLRNGAAGALRNLDIKETARRNLSAFFYDVGYNEGPEFKSYR
EMMNFIKNMGLPQDKYIKECTNMEEVEKEIEYIESIRGELDYDIDGAVIVVDDIKTREILGYTIKFPKWAIAYKFEAKEI
TTKLLDVEWNVGRSGRVTPTALLEPVELGGVTVKRATLNNMDDIKRKNVKLGAKVLVRRSNDVIPEIMGVVEESLEESEE
IQAPDRCPYCNSHLVQNGVHYYCENTLSCKPQMVKSIVHFASREAMNIAGFSEKTAEQLFEKLDIKSIADLYKIKKEELL
TLEKFKDKKSQNLIDAIQNSKNCDLASFIYALGIPNVGKKTANDLVMKFKTLESIKNTTIEQLVEVPDVGEIVAKSIYDF
FEDEKIISNIEELLNLGVKPYYEEERIDENPFMGKTIVVTGSLNNYSRGEIKDKLQSLGAKVSSSVSKNTDYVLVGEKPG
SKYEKAIELGVKVINEEEFSNKIK

Sequences:

>Translated_664_residues
MDNKLEKMKELVEELNQYAYEYYVLDNPSISDKEYDLKYDELVILEKKTEVTLPYSPTQRVGDKILGEFSKYTHKGRLWS
LDKAQNMEQLIEWHNRNLKVIEQYNSMSEDKLPELRYIVTKKFDGLTVNCTYDENGILIKSATRGTGIIGEDITAQIKTI
KTVPLKIKNNHVIEVHGEAIMTKTAFEEYNKAAQVPLKNLRNGAAGALRNLDIKETARRNLSAFFYDVGYNEGPEFKSYR
EMMNFIKNMGLPQDKYIKECTNMEEVEKEIEYIESIRGELDYDIDGAVIVVDDIKTREILGYTIKFPKWAIAYKFEAKEI
TTKLLDVEWNVGRSGRVTPTALLEPVELGGVTVKRATLNNMDDIKRKNVKLGAKVLVRRSNDVIPEIMGVVEESLEESEE
IQAPDRCPYCNSHLVQNGVHYYCENTLSCKPQMVKSIVHFASREAMNIAGFSEKTAEQLFEKLDIKSIADLYKIKKEELL
TLEKFKDKKSQNLIDAIQNSKNCDLASFIYALGIPNVGKKTANDLVMKFKTLESIKNTTIEQLVEVPDVGEIVAKSIYDF
FEDEKIISNIEELLNLGVKPYYEEERIDENPFMGKTIVVTGSLNNYSRGEIKDKLQSLGAKVSSSVSKNTDYVLVGEKPG
SKYEKAIELGVKVINEEEFSNKIK
>Mature_664_residues
MDNKLEKMKELVEELNQYAYEYYVLDNPSISDKEYDLKYDELVILEKKTEVTLPYSPTQRVGDKILGEFSKYTHKGRLWS
LDKAQNMEQLIEWHNRNLKVIEQYNSMSEDKLPELRYIVTKKFDGLTVNCTYDENGILIKSATRGTGIIGEDITAQIKTI
KTVPLKIKNNHVIEVHGEAIMTKTAFEEYNKAAQVPLKNLRNGAAGALRNLDIKETARRNLSAFFYDVGYNEGPEFKSYR
EMMNFIKNMGLPQDKYIKECTNMEEVEKEIEYIESIRGELDYDIDGAVIVVDDIKTREILGYTIKFPKWAIAYKFEAKEI
TTKLLDVEWNVGRSGRVTPTALLEPVELGGVTVKRATLNNMDDIKRKNVKLGAKVLVRRSNDVIPEIMGVVEESLEESEE
IQAPDRCPYCNSHLVQNGVHYYCENTLSCKPQMVKSIVHFASREAMNIAGFSEKTAEQLFEKLDIKSIADLYKIKKEELL
TLEKFKDKKSQNLIDAIQNSKNCDLASFIYALGIPNVGKKTANDLVMKFKTLESIKNTTIEQLVEVPDVGEIVAKSIYDF
FEDEKIISNIEELLNLGVKPYYEEERIDENPFMGKTIVVTGSLNNYSRGEIKDKLQSLGAKVSSSVSKNTDYVLVGEKPG
SKYEKAIELGVKVINEEEFSNKIK

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=679, Percent_Identity=40.6480117820324, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI87082305, Length=450, Percent_Identity=22.2222222222222, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; PF00633 HHH [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 75805; Mature: 75805

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50172 BRCT ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNKLEKMKELVEELNQYAYEYYVLDNPSISDKEYDLKYDELVILEKKTEVTLPYSPTQR
CCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCEEEEECCCCEECCCCCHHH
VGDKILGEFSKYTHKGRLWSLDKAQNMEQLIEWHNRNLKVIEQYNSMSEDKLPELRYIVT
HHHHHHHHHHHHCCCCCEEECHHHCCHHHHHHHHCCCCEEHHHHCCCCCCCCCHHHHHHE
KKFDGLTVNCTYDENGILIKSATRGTGIIGEDITAQIKTIKTVPLKIKNNHVIEVHGEAI
ECCCCEEEEEEECCCCEEEEECCCCCCCCCCCHHHHEEEEEEEEEEECCCEEEEECCCCH
MTKTAFEEYNKAAQVPLKNLRNGAAGALRNLDIKETARRNLSAFFYDVGYNEGPEFKSYR
HHHHHHHHHHHHHCCCHHHHCCCHHHHHHCCCHHHHHHCCCCEEEEECCCCCCCCHHHHH
EMMNFIKNMGLPQDKYIKECTNMEEVEKEIEYIESIRGELDYDIDGAVIVVDDIKTREIL
HHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHH
GYTIKFPKWAIAYKFEAKEITTKLLDVEWNVGRSGRVTPTALLEPVELGGVTVKRATLNN
EEEEECCCEEEEEEEEHHHHHHHHHEEEECCCCCCCCCHHHHCCCHHHCCEEEEEHHHCC
MDDIKRKNVKLGAKVLVRRSNDVIPEIMGVVEESLEESEEIQAPDRCPYCNSHLVQNGVH
HHHHHHHCHHHCHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCC
YYCENTLSCKPQMVKSIVHFASREAMNIAGFSEKTAEQLFEKLDIKSIADLYKIKKEELL
EEECCCCCCCHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHH
TLEKFKDKKSQNLIDAIQNSKNCDLASFIYALGIPNVGKKTANDLVMKFKTLESIKNTTI
HHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EQLVEVPDVGEIVAKSIYDFFEDEKIISNIEELLNLGVKPYYEEERIDENPFMGKTIVVT
HHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCEEEEE
GSLNNYSRGEIKDKLQSLGAKVSSSVSKNTDYVLVGEKPGSKYEKAIELGVKVINEEEFS
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCHHHHH
NKIK
CCCC
>Mature Secondary Structure
MDNKLEKMKELVEELNQYAYEYYVLDNPSISDKEYDLKYDELVILEKKTEVTLPYSPTQR
CCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCEEEEECCCCEECCCCCHHH
VGDKILGEFSKYTHKGRLWSLDKAQNMEQLIEWHNRNLKVIEQYNSMSEDKLPELRYIVT
HHHHHHHHHHHHCCCCCEEECHHHCCHHHHHHHHCCCCEEHHHHCCCCCCCCCHHHHHHE
KKFDGLTVNCTYDENGILIKSATRGTGIIGEDITAQIKTIKTVPLKIKNNHVIEVHGEAI
ECCCCEEEEEEECCCCEEEEECCCCCCCCCCCHHHHEEEEEEEEEEECCCEEEEECCCCH
MTKTAFEEYNKAAQVPLKNLRNGAAGALRNLDIKETARRNLSAFFYDVGYNEGPEFKSYR
HHHHHHHHHHHHHCCCHHHHCCCHHHHHHCCCHHHHHHCCCCEEEEECCCCCCCCHHHHH
EMMNFIKNMGLPQDKYIKECTNMEEVEKEIEYIESIRGELDYDIDGAVIVVDDIKTREIL
HHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHH
GYTIKFPKWAIAYKFEAKEITTKLLDVEWNVGRSGRVTPTALLEPVELGGVTVKRATLNN
EEEEECCCEEEEEEEEHHHHHHHHHEEEECCCCCCCCCHHHHCCCHHHCCEEEEEHHHCC
MDDIKRKNVKLGAKVLVRRSNDVIPEIMGVVEESLEESEEIQAPDRCPYCNSHLVQNGVH
HHHHHHHCHHHCHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCC
YYCENTLSCKPQMVKSIVHFASREAMNIAGFSEKTAEQLFEKLDIKSIADLYKIKKEELL
EEECCCCCCCHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHH
TLEKFKDKKSQNLIDAIQNSKNCDLASFIYALGIPNVGKKTANDLVMKFKTLESIKNTTI
HHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EQLVEVPDVGEIVAKSIYDFFEDEKIISNIEELLNLGVKPYYEEERIDENPFMGKTIVVT
HHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCEEEEE
GSLNNYSRGEIKDKLQSLGAKVSSSVSKNTDYVLVGEKPGSKYEKAIELGVKVINEEEFS
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCHHHHH
NKIK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA