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The map label for this gene is gatA [H]

Identifier: 226950692

GI number: 226950692

Start: 3712657

End: 3714114

Strand: Reverse

Name: gatA [H]

Synonym: CLM_3700

Alternate gene names: 226950692

Gene position: 3714114-3712657 (Counterclockwise)

Preceding gene: 226950693

Following gene: 226950691

Centisome position: 89.38

GC content: 32.1

Gene sequence:

>1458_bases
ATGGATTTAACTAAATTAACAGCACACGAATTAAAAGATATACTTTCAAATAAAGAAGTTAAAGCAGAAGAAATAACAAG
AGCTTTTTTAGATAGAATAAATTTAGTAGACAATAAGCTAGGAGCATACTTATATGTATCAGAAGAAGAAGCTATAAAAA
AAGCTAAAGAAATTGATGTAAAAATAGAAAAAAATGAAGAGTTAAAAGCCTTATCAGGTATACCAGTAGGTATAAAAGAT
AATATAAATGTAAAAGGAATGCAGAATACTTGTGCATCTAAAATATTACAAGGATATACTTCACCTTATGATGCTCATGT
AACAGAGAAAATAAAAAAAGAAGAGGGAATAATCCTTGGCAAATTAAACATGGATGAGTTTGCTATGGGATCATCTACAG
AAAATAGTGCATTTAAATTAGCTAAGAATCCATGGGATTTAGAAAGAGTGCCAGGAGGATCTTCAGGTGGATCTGCAGTA
GCAGTAGCAGGATGTGAAGCGACCTTATCACTAGGGACAGATACTGGTGGTTCTGTAAGACAACCAGCATCTTTTTGTGG
AATAGTTGGGTTAAAACCTACATATGGAAGAATATCAAGATCAGGGGTTGTGGCTTTTGGTTCCACATTAGATCAAGTAG
GTCCAATGGGAAAAGATGTAGAAGATTGTGCATTACTCACATCAGCTATAGCAGGATTAGATAAAAAGGATTTTACAACA
GCAGATAAGGAAGTACCAGATTATAAAAAAAGCTTAACTAAAGATATAAAAGGTAAAAAGATTGGTATTCCAAAGGAGTT
TTTTGGAGAAGGTCTAGATGAGAAAGTAAGAAAATCTGTAGAAGAAGCCATAAAGGTATTAGAGGAAAATGGAGCGGAAG
TTAAACCATGTTCTCTACCACTTATGGATTATGCTCTATCAGCTTATTATATAATATCTAGTGCAGAAGCTTCATCAAAC
TTAGCTAGATTTGATGGTATAAGATACGGGCATAGATCGAAAAACTTTAAAGATGCTCAGGATATATATTTAAAATCTAG
AAGTGAAGGGTTTGGAGATGAAGTTAAGAGAAGAATAATGCTTGGAACTTATGTATTATCAGCAGGATATTATGATGCTT
ATTATAAAAAAGCATTAAAGGTAAGAAAACTTATAAAAGACGATTTCCAAAGAGTATTTAAAGACTTTGATGCTATAGTG
TCACCAACATCTCCAACTACAGCATTTAAAGTAGGAGAAAAGAAAGACGATGTAATGTCAATGTATCTTTCAGATATATA
TACTGTTCCAATTAGTGTAGCAGGAGTACCAGCTATATCATTACCATGTGGTATGATAGATGGACTTCCAGTAGGGCTTC
AAATTATATCAGATTATTTTAAAGAAGATGTATTATTTAATTTAGCATATAATTATGAACAATCAGTAGATTTTCATAAA
ATGAGAGCGGATTTTTAA

Upstream 100 bases:

>100_bases
AGTTATAGATAATGCACCAGAAAGCTTAGAAGAATATGTAATTGTTCCTAAAGTTATAGATTAATAATTCTTTTGTTTTT
TGGAAAAAGGAGGAAAGAAA

Downstream 100 bases:

>100_bases
GTATATAGTTAAAAACAAAGGAGTGAAAAACATAGATGGATTTTGAAGCAGTTATAGGATTGGAAGTTCATGCTGAACTT
TCAACAAATACTAAAATATA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MDLTKLTAHELKDILSNKEVKAEEITRAFLDRINLVDNKLGAYLYVSEEEAIKKAKEIDVKIEKNEELKALSGIPVGIKD
NINVKGMQNTCASKILQGYTSPYDAHVTEKIKKEEGIILGKLNMDEFAMGSSTENSAFKLAKNPWDLERVPGGSSGGSAV
AVAGCEATLSLGTDTGGSVRQPASFCGIVGLKPTYGRISRSGVVAFGSTLDQVGPMGKDVEDCALLTSAIAGLDKKDFTT
ADKEVPDYKKSLTKDIKGKKIGIPKEFFGEGLDEKVRKSVEEAIKVLEENGAEVKPCSLPLMDYALSAYYIISSAEASSN
LARFDGIRYGHRSKNFKDAQDIYLKSRSEGFGDEVKRRIMLGTYVLSAGYYDAYYKKALKVRKLIKDDFQRVFKDFDAIV
SPTSPTTAFKVGEKKDDVMSMYLSDIYTVPISVAGVPAISLPCGMIDGLPVGLQIISDYFKEDVLFNLAYNYEQSVDFHK
MRADF

Sequences:

>Translated_485_residues
MDLTKLTAHELKDILSNKEVKAEEITRAFLDRINLVDNKLGAYLYVSEEEAIKKAKEIDVKIEKNEELKALSGIPVGIKD
NINVKGMQNTCASKILQGYTSPYDAHVTEKIKKEEGIILGKLNMDEFAMGSSTENSAFKLAKNPWDLERVPGGSSGGSAV
AVAGCEATLSLGTDTGGSVRQPASFCGIVGLKPTYGRISRSGVVAFGSTLDQVGPMGKDVEDCALLTSAIAGLDKKDFTT
ADKEVPDYKKSLTKDIKGKKIGIPKEFFGEGLDEKVRKSVEEAIKVLEENGAEVKPCSLPLMDYALSAYYIISSAEASSN
LARFDGIRYGHRSKNFKDAQDIYLKSRSEGFGDEVKRRIMLGTYVLSAGYYDAYYKKALKVRKLIKDDFQRVFKDFDAIV
SPTSPTTAFKVGEKKDDVMSMYLSDIYTVPISVAGVPAISLPCGMIDGLPVGLQIISDYFKEDVLFNLAYNYEQSVDFHK
MRADF
>Mature_485_residues
MDLTKLTAHELKDILSNKEVKAEEITRAFLDRINLVDNKLGAYLYVSEEEAIKKAKEIDVKIEKNEELKALSGIPVGIKD
NINVKGMQNTCASKILQGYTSPYDAHVTEKIKKEEGIILGKLNMDEFAMGSSTENSAFKLAKNPWDLERVPGGSSGGSAV
AVAGCEATLSLGTDTGGSVRQPASFCGIVGLKPTYGRISRSGVVAFGSTLDQVGPMGKDVEDCALLTSAIAGLDKKDFTT
ADKEVPDYKKSLTKDIKGKKIGIPKEFFGEGLDEKVRKSVEEAIKVLEENGAEVKPCSLPLMDYALSAYYIISSAEASSN
LARFDGIRYGHRSKNFKDAQDIYLKSRSEGFGDEVKRRIMLGTYVLSAGYYDAYYKKALKVRKLIKDDFQRVFKDFDAIV
SPTSPTTAFKVGEKKDDVMSMYLSDIYTVPISVAGVPAISLPCGMIDGLPVGLQIISDYFKEDVLFNLAYNYEQSVDFHK
MRADF

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=467, Percent_Identity=36.6167023554604, Blast_Score=305, Evalue=5e-83,
Organism=Homo sapiens, GI195972892, Length=497, Percent_Identity=24.1448692152917, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI166795287, Length=214, Percent_Identity=29.9065420560748, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=435, Percent_Identity=38.1609195402299, Blast_Score=294, Evalue=6e-80,
Organism=Caenorhabditis elegans, GI17537465, Length=499, Percent_Identity=24.4488977955912, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI71990152, Length=314, Percent_Identity=29.9363057324841, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17556264, Length=293, Percent_Identity=28.3276450511945, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17538252, Length=317, Percent_Identity=26.813880126183, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=300, Percent_Identity=27, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=300, Percent_Identity=27, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=412, Percent_Identity=36.4077669902913, Blast_Score=227, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6319685, Length=458, Percent_Identity=27.9475982532751, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320448, Length=525, Percent_Identity=24.1904761904762, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648113, Length=464, Percent_Identity=38.3620689655172, Blast_Score=310, Evalue=2e-84,
Organism=Drosophila melanogaster, GI45550774, Length=497, Percent_Identity=25.9557344064386, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24648435, Length=496, Percent_Identity=26.008064516129, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24648437, Length=496, Percent_Identity=26.008064516129, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24652985, Length=491, Percent_Identity=24.4399185336049, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI19922090, Length=491, Percent_Identity=24.4399185336049, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24652981, Length=491, Percent_Identity=24.4399185336049, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24652983, Length=491, Percent_Identity=24.4399185336049, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24644968, Length=519, Percent_Identity=25.4335260115607, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21356731, Length=490, Percent_Identity=26.1224489795918, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24648441, Length=430, Percent_Identity=26.046511627907, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24648439, Length=430, Percent_Identity=26.046511627907, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI161078093, Length=515, Percent_Identity=24.6601941747573, Blast_Score=107, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53227; Mature: 53227

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLTKLTAHELKDILSNKEVKAEEITRAFLDRINLVDNKLGAYLYVSEEEAIKKAKEIDV
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHCCE
KIEKNEELKALSGIPVGIKDNINVKGMQNTCASKILQGYTSPYDAHVTEKIKKEEGIILG
EEECCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEE
KLNMDEFAMGSSTENSAFKLAKNPWDLERVPGGSSGGSAVAVAGCEATLSLGTDTGGSVR
ECCCHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCCCCC
QPASFCGIVGLKPTYGRISRSGVVAFGSTLDQVGPMGKDVEDCALLTSAIAGLDKKDFTT
CCHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC
ADKEVPDYKKSLTKDIKGKKIGIPKEFFGEGLDEKVRKSVEEAIKVLEENGAEVKPCSLP
CCCCCCHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
LMDYALSAYYIISSAEASSNLARFDGIRYGHRSKNFKDAQDIYLKSRSEGFGDEVKRRIM
HHHHHHHHHHEEECCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHH
LGTYVLSAGYYDAYYKKALKVRKLIKDDFQRVFKDFDAIVSPTSPTTAFKVGEKKDDVMS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHH
MYLSDIYTVPISVAGVPAISLPCGMIDGLPVGLQIISDYFKEDVLFNLAYNYEQSVDFHK
HHHHHHHHCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCHHH
MRADF
HHCCC
>Mature Secondary Structure
MDLTKLTAHELKDILSNKEVKAEEITRAFLDRINLVDNKLGAYLYVSEEEAIKKAKEIDV
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHCCE
KIEKNEELKALSGIPVGIKDNINVKGMQNTCASKILQGYTSPYDAHVTEKIKKEEGIILG
EEECCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEE
KLNMDEFAMGSSTENSAFKLAKNPWDLERVPGGSSGGSAVAVAGCEATLSLGTDTGGSVR
ECCCHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCCCCC
QPASFCGIVGLKPTYGRISRSGVVAFGSTLDQVGPMGKDVEDCALLTSAIAGLDKKDFTT
CCHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC
ADKEVPDYKKSLTKDIKGKKIGIPKEFFGEGLDEKVRKSVEEAIKVLEENGAEVKPCSLP
CCCCCCHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
LMDYALSAYYIISSAEASSNLARFDGIRYGHRSKNFKDAQDIYLKSRSEGFGDEVKRRIM
HHHHHHHHHHEEECCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHH
LGTYVLSAGYYDAYYKKALKVRKLIKDDFQRVFKDFDAIVSPTSPTTAFKVGEKKDDVMS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHH
MYLSDIYTVPISVAGVPAISLPCGMIDGLPVGLQIISDYFKEDVLFNLAYNYEQSVDFHK
HHHHHHHHCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCHHH
MRADF
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA