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The map label for this gene is lonB [H]

Identifier: 226950657

GI number: 226950657

Start: 3680242

End: 3681921

Strand: Reverse

Name: lonB [H]

Synonym: CLM_3641

Alternate gene names: 226950657

Gene position: 3681921-3680242 (Counterclockwise)

Preceding gene: 226950658

Following gene: 226950656

Centisome position: 88.61

GC content: 30.3

Gene sequence:

>1680_bases
ATGACGCAAACATTAATATTAATAGAAATATTTTTTACAGTTATTATGGGACTATATTTTATGAATGCTTTAAAAAGTCA
AAAATCTAATAAAGTAGTGATTGAAAAAGAAAATAAAAAGGAAATAGACACATTAAAAAAATTGAGAAGTATAAAATTAA
CAGAACCTTTAACAGAAAAAAGTAGACCTTCATCTTTTGATGAAATAATAGGTCAAGAAAATGGAATAAAAGCACTAAAA
GCTGCTATATGCAGTCCTAATCCACAACATGTAATAATATATGGACCACCAGGAACAGGAAAAACAGCAGCAGCTAGATT
AATTTTAGAAGATGCAAAGAAAAAAGAATATTCCCCATTTAATCAACAATCTAAATTTATGGAAATAGATGCTACTACAG
TGAGATCAGATGAAAGAGGAATAGCAGATCCTTTAATAGGTTCAGTTCATGATCCTATTTATCAAGGAGCAGGAGCATTA
GGTCTGGCAGGAGTACCTCAACCAAAGCCGGGTGCAGTAACTAAGGCTCATGGAGGAATATTGTTTATAGATGAAATAGG
AGAACTTCATCCTAGTGAAATGAATAAATTATTAAAAGTTTTAGAGGACAGAAAAGTTTTTTTAGATAGTGCTTATTATA
ATCCCTCAGATAATAATATACCAAGTTATATAAAAGATATATTTGAAAATGGTTTGCCAGCGGATTTTAGATTAATAGGG
GCTACCACAAGAAGTCCAGAGGAAATAACTCCAGCTTTAAGATCTAGATGTGTAGAAGTGTTTTTTAGAGCTCTATTACC
AGAAGAAATAAAGAAAATAGCTATGAATGCTATTAAAAAATTAAATTTTACTATAGAAGAAGAGGCTTGTGAATTAATAT
CCAAATATTGTAGTAATGGTAGAGATACTATAAATTTAATACAATTATCTGCAGGTTTATCAATAAATAGTAATAGAAAT
AAGATATTAATACAGGATGTAGAATGGGTTATAGAAAATGGTCAATATTCTCCAAGAATACAAAGGAAGATACCATTAGA
ACCTAAAGTTGGATATGTAAATGGATTAGCAGTATATGGAGCTAATATGGGCATGGTCATGGAGATAGAGGCAACAGCTA
AGAAAATAAATTTTAGAAAAGGAAATTTATCTATAACAGGATTAGTGGAAGAGGAAGAAATTAATAGTTCTAATAAAAAA
GCAATTAGAAAAAGTACTGCATGGTGTTCTGTTCAAAATGTTATTACTATAATGAAACAAATGTTTGATTTAGAGTGCGA
TGAATATGATATACATATAAATTTTCCAGGTGGTATACCTGTAGATGGACCATCAGCGGGTATAAGTATAACTACAGCGG
TATATAGTGCTATTATAGGTAAACCTGTAGATAATAATGTAGCTATGACTGGAGAGATATCTATTCATGGAGAAGTAAAA
CCTGTAGGTGGGGTAAGCTCTAAAGTAGAGGCTGCTATTAAAGCTGGAGCGAAAAAAATCATAATACCTAAGGAAAATTG
GCAGGATAAATTTGAAAAATTAGATAAGGTAAAAGTGATACCTGTTTCAGATATAAAAACAGTAATAAAAGAGGCTATTT
GTGTTGGGGAAGAAGATAAATTATTTTTAAATATAGATAAGAGCGTAGAATTTTTGTCAGCAGAATCTATGACTAAATAA

Upstream 100 bases:

>100_bases
TTATTTTTTACTATTATATTAAATGAATAAAATGCATTTTTGAATATATAAAGAGGAAAAAGAACATAATAATAAAAAAA
GTATTAAAGGGGTTTTGGAT

Downstream 100 bases:

>100_bases
AATTCATATAATTAAGTATAGCTTTAAAAAAATAATAAATAACCTTATAATAACTATTTGAAAAAAAGTATTTTTATAGG
TATAATATATTAGTCTAGCT

Product: ATP-dependent protease LonB

Products: NA

Alternate protein names: ATP-dependent protease La 2 [H]

Number of amino acids: Translated: 559; Mature: 558

Protein sequence:

>559_residues
MTQTLILIEIFFTVIMGLYFMNALKSQKSNKVVIEKENKKEIDTLKKLRSIKLTEPLTEKSRPSSFDEIIGQENGIKALK
AAICSPNPQHVIIYGPPGTGKTAAARLILEDAKKKEYSPFNQQSKFMEIDATTVRSDERGIADPLIGSVHDPIYQGAGAL
GLAGVPQPKPGAVTKAHGGILFIDEIGELHPSEMNKLLKVLEDRKVFLDSAYYNPSDNNIPSYIKDIFENGLPADFRLIG
ATTRSPEEITPALRSRCVEVFFRALLPEEIKKIAMNAIKKLNFTIEEEACELISKYCSNGRDTINLIQLSAGLSINSNRN
KILIQDVEWVIENGQYSPRIQRKIPLEPKVGYVNGLAVYGANMGMVMEIEATAKKINFRKGNLSITGLVEEEEINSSNKK
AIRKSTAWCSVQNVITIMKQMFDLECDEYDIHINFPGGIPVDGPSAGISITTAVYSAIIGKPVDNNVAMTGEISIHGEVK
PVGGVSSKVEAAIKAGAKKIIIPKENWQDKFEKLDKVKVIPVSDIKTVIKEAICVGEEDKLFLNIDKSVEFLSAESMTK

Sequences:

>Translated_559_residues
MTQTLILIEIFFTVIMGLYFMNALKSQKSNKVVIEKENKKEIDTLKKLRSIKLTEPLTEKSRPSSFDEIIGQENGIKALK
AAICSPNPQHVIIYGPPGTGKTAAARLILEDAKKKEYSPFNQQSKFMEIDATTVRSDERGIADPLIGSVHDPIYQGAGAL
GLAGVPQPKPGAVTKAHGGILFIDEIGELHPSEMNKLLKVLEDRKVFLDSAYYNPSDNNIPSYIKDIFENGLPADFRLIG
ATTRSPEEITPALRSRCVEVFFRALLPEEIKKIAMNAIKKLNFTIEEEACELISKYCSNGRDTINLIQLSAGLSINSNRN
KILIQDVEWVIENGQYSPRIQRKIPLEPKVGYVNGLAVYGANMGMVMEIEATAKKINFRKGNLSITGLVEEEEINSSNKK
AIRKSTAWCSVQNVITIMKQMFDLECDEYDIHINFPGGIPVDGPSAGISITTAVYSAIIGKPVDNNVAMTGEISIHGEVK
PVGGVSSKVEAAIKAGAKKIIIPKENWQDKFEKLDKVKVIPVSDIKTVIKEAICVGEEDKLFLNIDKSVEFLSAESMTK
>Mature_558_residues
TQTLILIEIFFTVIMGLYFMNALKSQKSNKVVIEKENKKEIDTLKKLRSIKLTEPLTEKSRPSSFDEIIGQENGIKALKA
AICSPNPQHVIIYGPPGTGKTAAARLILEDAKKKEYSPFNQQSKFMEIDATTVRSDERGIADPLIGSVHDPIYQGAGALG
LAGVPQPKPGAVTKAHGGILFIDEIGELHPSEMNKLLKVLEDRKVFLDSAYYNPSDNNIPSYIKDIFENGLPADFRLIGA
TTRSPEEITPALRSRCVEVFFRALLPEEIKKIAMNAIKKLNFTIEEEACELISKYCSNGRDTINLIQLSAGLSINSNRNK
ILIQDVEWVIENGQYSPRIQRKIPLEPKVGYVNGLAVYGANMGMVMEIEATAKKINFRKGNLSITGLVEEEEINSSNKKA
IRKSTAWCSVQNVITIMKQMFDLECDEYDIHINFPGGIPVDGPSAGISITTAVYSAIIGKPVDNNVAMTGEISIHGEVKP
VGGVSSKVEAAIKAGAKKIIIPKENWQDKFEKLDKVKVIPVSDIKTVIKEAICVGEEDKLFLNIDKSVEFLSAESMTK

Specific function: ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced

COG id: COG1067

COG function: function code O; Predicted ATP-dependent protease

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S16 family [H]

Homologues:

Organism=Homo sapiens, GI31377667, Length=150, Percent_Identity=38, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI21396489, Length=472, Percent_Identity=26.271186440678, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1786643, Length=480, Percent_Identity=27.5, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17505831, Length=482, Percent_Identity=25.3112033195021, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556486, Length=454, Percent_Identity=24.8898678414097, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319449, Length=89, Percent_Identity=46.0674157303371, Blast_Score=80, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6322528, Length=104, Percent_Identity=36.5384615384615, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24666867, Length=180, Percent_Identity=37.2222222222222, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI221513036, Length=180, Percent_Identity=37.2222222222222, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR008269
- InterPro:   IPR008268
- InterPro:   IPR001984
- InterPro:   IPR020568
- InterPro:   IPR014251 [H]

Pfam domain/function: PF00004 AAA; PF05362 Lon_C [H]

EC number: =3.4.21.53 [H]

Molecular weight: Translated: 61629; Mature: 61498

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTLILIEIFFTVIMGLYFMNALKSQKSNKVVIEKENKKEIDTLKKLRSIKLTEPLTEK
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCC
SRPSSFDEIIGQENGIKALKAAICSPNPQHVIIYGPPGTGKTAAARLILEDAKKKEYSPF
CCCCCHHHHHCCCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCC
NQQSKFMEIDATTVRSDERGIADPLIGSVHDPIYQGAGALGLAGVPQPKPGAVTKAHGGI
CCCCCEEEEEHHEECCCCCCCCCHHHHHHCCHHHHCCCCCCCCCCCCCCCCCEEECCCCE
LFIDEIGELHPSEMNKLLKVLEDRKVFLDSAYYNPSDNNIPSYIKDIFENGLPADFRLIG
EEEECCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHCCCCCCEEEEE
ATTRSPEEITPALRSRCVEVFFRALLPEEIKKIAMNAIKKLNFTIEEEACELISKYCSNG
CCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHCCC
RDTINLIQLSAGLSINSNRNKILIQDVEWVIENGQYSPRIQRKIPLEPKVGYVNGLAVYG
CCEEEEEEEECCCEECCCCCEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEC
ANMGMVMEIEATAKKINFRKGNLSITGLVEEEEINSSNKKAIRKSTAWCSVQNVITIMKQ
CCCCEEEEEEHHHHHEEEEECCEEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
MFDLECDEYDIHINFPGGIPVDGPSAGISITTAVYSAIIGKPVDNNVAMTGEISIHGEVK
HHCCCCCCEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCEEEEEEEEEECCCC
PVGGVSSKVEAAIKAGAKKIIIPKENWQDKFEKLDKVKVIPVSDIKTVIKEAICVGEEDK
CCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCCC
LFLNIDKSVEFLSAESMTK
EEEEECCCHHHHHHHCCCC
>Mature Secondary Structure 
TQTLILIEIFFTVIMGLYFMNALKSQKSNKVVIEKENKKEIDTLKKLRSIKLTEPLTEK
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCC
SRPSSFDEIIGQENGIKALKAAICSPNPQHVIIYGPPGTGKTAAARLILEDAKKKEYSPF
CCCCCHHHHHCCCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCC
NQQSKFMEIDATTVRSDERGIADPLIGSVHDPIYQGAGALGLAGVPQPKPGAVTKAHGGI
CCCCCEEEEEHHEECCCCCCCCCHHHHHHCCHHHHCCCCCCCCCCCCCCCCCEEECCCCE
LFIDEIGELHPSEMNKLLKVLEDRKVFLDSAYYNPSDNNIPSYIKDIFENGLPADFRLIG
EEEECCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHCCCCCCEEEEE
ATTRSPEEITPALRSRCVEVFFRALLPEEIKKIAMNAIKKLNFTIEEEACELISKYCSNG
CCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHCCC
RDTINLIQLSAGLSINSNRNKILIQDVEWVIENGQYSPRIQRKIPLEPKVGYVNGLAVYG
CCEEEEEEEECCCEECCCCCEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEC
ANMGMVMEIEATAKKINFRKGNLSITGLVEEEEINSSNKKAIRKSTAWCSVQNVITIMKQ
CCCCEEEEEEHHHHHEEEEECCEEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
MFDLECDEYDIHINFPGGIPVDGPSAGISITTAVYSAIIGKPVDNNVAMTGEISIHGEVK
HHCCCCCCEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCEEEEEEEEEECCCC
PVGGVSSKVEAAIKAGAKKIIIPKENWQDKFEKLDKVKVIPVSDIKTVIKEAICVGEEDK
CCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCCC
LFLNIDKSVEFLSAESMTK
EEEEECCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 7961402; 11325926 [H]