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The map label for this gene is pflA [H]

Identifier: 226950642

GI number: 226950642

Start: 3665092

End: 3665790

Strand: Reverse

Name: pflA [H]

Synonym: CLM_3626

Alternate gene names: 226950642

Gene position: 3665790-3665092 (Counterclockwise)

Preceding gene: 226950643

Following gene: 226950641

Centisome position: 88.22

GC content: 29.04

Gene sequence:

>699_bases
ATGGGTAAAATTCATTCAATAGAAACTATGGGACTTGTAGATGGTCCAGGGATTAGAGTAGTAGTATTTTTTCAAGGTTG
CCAATTAAGATGTGTCTATTGTCATAATCCGGATACATGGGATTTTAATGCTGGTATAGAGATTAGTAGTGATGAAGTAT
TAAAAAAAGTATTAAGATATAAGCCATATTTTAAACAGGTTGGAGGAATAACGTGTTCAGGAGGAGAGCCTTTAATGCAA
CCTGAATTTCTTTTAGAAATTTTAAAAAAATGTGAAAATCAAAGCATCCATACAGCATTAGATACATCAGGAGTAGGAAT
CGGAAATTATGAAGAGATACTTCAATATGTAGATTTAGTTATATTAGATATTAAGCACATAGAGGAAGAAAAATATATTG
GTATTTGCGGGAAAAATATGGAGGAATTCAACAAATTTAAACGTGTTGTAAATAAACTTAATAAAAAATTATGGATAAGG
CATGTGGTTGTTCCAGGGATAAATGATACTGGAGAGCATATATATAAATTTAAAGATTATATAAATACTTTTAACAACGT
AGAGAAGGTTGAATTATTGCCTTATCATACATTAGGGGTTAGTAAATATGAAAATATGGGAATAGAGTATAAACTTAAAA
ATACAAGTCCCTTGAGTAAAGATAAGCTTGAAGAATTAAAGAAAATTATTTCAATATAA

Upstream 100 bases:

>100_bases
TATAAGGGGTGTCATAGCCCTAGATAAGTTTTTCTAATACTCAGGTGGAGAGTAATATTCTTCATCTGAGTATTATAATT
ACTTGATAAGGAGATTTCAT

Downstream 100 bases:

>100_bases
AAATCATATTTAAATAATTTATTAAAACATAGTGAATGCTTATAAGTTTATAAAAGATTTAGAGCTTCTTATTTGTTTTT
TAATATATTATATATAAAAT

Product: pyruvate formate-lyase activating enzyme

Products: NA

Alternate protein names: PFL-activating enzyme; Formate-C-acetyltransferase-activating enzyme [H]

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MGKIHSIETMGLVDGPGIRVVVFFQGCQLRCVYCHNPDTWDFNAGIEISSDEVLKKVLRYKPYFKQVGGITCSGGEPLMQ
PEFLLEILKKCENQSIHTALDTSGVGIGNYEEILQYVDLVILDIKHIEEEKYIGICGKNMEEFNKFKRVVNKLNKKLWIR
HVVVPGINDTGEHIYKFKDYINTFNNVEKVELLPYHTLGVSKYENMGIEYKLKNTSPLSKDKLEELKKIISI

Sequences:

>Translated_232_residues
MGKIHSIETMGLVDGPGIRVVVFFQGCQLRCVYCHNPDTWDFNAGIEISSDEVLKKVLRYKPYFKQVGGITCSGGEPLMQ
PEFLLEILKKCENQSIHTALDTSGVGIGNYEEILQYVDLVILDIKHIEEEKYIGICGKNMEEFNKFKRVVNKLNKKLWIR
HVVVPGINDTGEHIYKFKDYINTFNNVEKVELLPYHTLGVSKYENMGIEYKLKNTSPLSKDKLEELKKIISI
>Mature_231_residues
GKIHSIETMGLVDGPGIRVVVFFQGCQLRCVYCHNPDTWDFNAGIEISSDEVLKKVLRYKPYFKQVGGITCSGGEPLMQP
EFLLEILKKCENQSIHTALDTSGVGIGNYEEILQYVDLVILDIKHIEEEKYIGICGKNMEEFNKFKRVVNKLNKKLWIRH
VVVPGINDTGEHIYKFKDYINTFNNVEKVELLPYHTLGVSKYENMGIEYKLKNTSPLSKDKLEELKKIISI

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=235, Percent_Identity=41.7021276595745, Blast_Score=206, Evalue=8e-55,
Organism=Escherichia coli, GI1790389, Length=254, Percent_Identity=25.1968503937008, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI226510931, Length=182, Percent_Identity=30.2197802197802, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 26605; Mature: 26474

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKIHSIETMGLVDGPGIRVVVFFQGCQLRCVYCHNPDTWDFNAGIEISSDEVLKKVLRY
CCCCCCEEEECCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCEECHHHHHHHHHHC
KPYFKQVGGITCSGGEPLMQPEFLLEILKKCENQSIHTALDTSGVGIGNYEEILQYVDLV
CHHHHHHCCEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHH
ILDIKHIEEEKYIGICGKNMEEFNKFKRVVNKLNKKLWIRHVVVPGINDTGEHIYKFKDY
HHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHH
INTFNNVEKVELLPYHTLGVSKYENMGIEYKLKNTSPLSKDKLEELKKIISI
HHHHCCCCEEEEECCHHCCCCHHHCCCEEEEECCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
GKIHSIETMGLVDGPGIRVVVFFQGCQLRCVYCHNPDTWDFNAGIEISSDEVLKKVLRY
CCCCCEEEECCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCEECHHHHHHHHHHC
KPYFKQVGGITCSGGEPLMQPEFLLEILKKCENQSIHTALDTSGVGIGNYEEILQYVDLV
CHHHHHHCCEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHH
ILDIKHIEEEKYIGICGKNMEEFNKFKRVVNKLNKKLWIRHVVVPGINDTGEHIYKFKDY
HHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHH
INTFNNVEKVELLPYHTLGVSKYENMGIEYKLKNTSPLSKDKLEELKKIISI
HHHHCCCCEEEEECCHHCCCCHHHCCCEEEEECCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8636053 [H]