| Definition | Clostridium botulinum A2 str. Kyoto chromosome, complete genome. |
|---|---|
| Accession | NC_012563 |
| Length | 4,155,278 |
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The map label for this gene is pflD [H]
Identifier: 226950611
GI number: 226950611
Start: 3630449
End: 3632812
Strand: Reverse
Name: pflD [H]
Synonym: CLM_3595
Alternate gene names: 226950611
Gene position: 3632812-3630449 (Counterclockwise)
Preceding gene: 226950612
Following gene: 226950610
Centisome position: 87.43
GC content: 31.35
Gene sequence:
>2364_bases ATGATGACAGACAGAGTTAAAAAATTAAGAGAAGAAAGTTTAAAGGCTGTACCACGTATTTCTATGGAGAGAACTAGAAT AGTTACTGATGTTTATAAAAAATATGAAGGAACAGTATCAATCCCAGTATTAAGAGCATTAGTTTTAAAGGAATTAATGG AAAGAAAAGAGCTTTGTATTTATGATGGAGAACTTATAGTAGGTGAAAGAGGGGAAGCTGCAGCAGCTACACCAACTTAT CCAGAATTATGCTGTCATACAGTTGAAGACTTTGATATAATGGATAAACGTGAAAAAATATCCTTTAAAACAACTGATGA AGATAAAGAAATACAAGAAGAATTAATTATTCCTTTCTGGGAAAAAAGATCTATGAGACATAAAATATTAGAAAAAATGA CTCCAGAATGGAAAGCTTGTTATGAAGCAGGTATATTCACCGAATTTATGGAACAAAGGGGACCAGGGCATACTGCAGGT GGAGATAAATATTATAAAATGGGATTTTTAGATATTAAAGAACAAATAAAAGAAGCTATAAGTAAATTAGATTATTTAAA TGATGATGAAGCATTAGATAAAAAAGAACAATTAGATGCTATGGATATAGCTTGTGATGCTATAATGATTTATGGAAAAC GTTATTCAGAATATGCAGCTAAATTAGCACAAAAAGAAGCAGATCCAGTAAGAAAAAAAGAACTTATGGAAATATCTGAA GTGTGTGGTTGGGTGCCAGCTCATGCTCCAAGAACCTTTAGAGAAGCAATTCAAATGTACTGGTTTGTTCATTTATGTGT AATTTCTGAATTAAATCCTTGGGATGCATTTAATCCTGGTAGATTAGATCAACATTTATATCCATTCTATAAAAAAGAAA TAGAAGAGGGAACATTAGATAGGGAGCAGGCTAGAGAATTGTTGCAATGTTTCTGGGTTAAATTTAATAACCAACCAGCG CCACCAAAAGTTGGTATAACTTTAAAAGAAAGTGGAACATATACTGACTTTGCAAACATAAATAGTGGTGGTATGAAAGC TGATGGATCAGATGGTGTTAATGATGTAAGCTATTTAGTACTTGAAGTAATAGATGAAATGAAGTTATTGCAACCAAGTT CTAATGTACAGATAAGTAAAAAAACCCCTCAAAGATTTTTAAAGAAGGCTTGCGAAGTTATAAGAAAAGGATGGGGCCAA CCATCAATATTTAATGCTGACTCAGTAGTTCAAGAATTAGTAAGAGCAGGAAAATCTATAGAAGATGCTAGATGCGGAGG AACTAGTGGTTGTGTTGAAGCTGGAGCTTTTGGTAAAGAAGCTTATATATTAACTGGATACTTTAATTTACCAAAAATAT TAGAAATAACATTATCAAATGGTGTGGATTCTCAAACTGGTAAACAATTAGGTATAAAAACAGGAGATATAAGTACATTA AAAACTTATGAAGATTTACTAGATGCATTTAAAAAACAACTTAAATATTTTGTTGATATAAAAGTAAATGGTAACAGAGT TATAGAAAGGTTATATGCAACATTGATGCCTGCACCATTCTTATCAGTTGTTACAGATGATTGTATAGCAAAAGGAAAAG ATTATAATGCAGGAGGAGCAAGATATAATACTAGTTATATTCAAGGTGTTGGTATAGGGACAATTACAGACAGTTTATCA GCAATCAAATACCAAGTATTTGATGAAAAGAATATAACAATGGAAGAATTAATGGAAGCATTAAGATCTAATTTTGAAGG ACATGAAGATATATATAACTTAGTTAAGAATAAAACTCCTAAATATGGTAATGATGATGACTATGCAGATGAAATAATGA AAGAAGTATTTGATGCTTATTATAATGAAGTAAATGGAAGACCTAATGGTAGAGGAGGATGCTATAGAATAGATATGCTA CCAACAACATGCCATGTTTACTTTGGATCAGTTATAAATGCTACTCCAGATGGAAGAAAAGCTCATATTCCAGTATCAGA AGGTATTTCTCCATCAAAGGGTGCAGATGTAAATGGACCAACAGGCGTTATAAAGTCAGCAGCTAAAATGGATCATTTAA GAACTGGTGGTACTTTATTGAATCAAAAATTTGTTCCTTCAGTTGTTCAAGGTGAAGAAGGAATAGATAATATGGCTAAT TTAGTAAGAGCTTATTTTACAATGGACGGGCATCACATTCAATTTAATATTGTAAGTAAGGAAACATTATTAAAAGCTCA ACAAAATCCGGATGAATATAAAGATTTAATAGTTCGTGTTGCTGGATATAGCGACTACTTTAATAACTTAGATAAAGTTT TACAAAATGAGATAATAGAAAGAACAGAACAAGAGTTTAATTAA
Upstream 100 bases:
>100_bases AATTAACTGGTGAGGAAAAACCATCAGATGAGAAAATGAATGAGATAGCAGAAAAATTTAAGCAGGCTGGAATTAAAGTA AAAATAGGGGGTAATTAATT
Downstream 100 bases:
>100_bases AAACTAGTTTAATATATTATTGTAAAGAAAAATAAAAGCTAGGAGAAGGAACTAAGATTTATAATTTAAGTCTTTCTCCT AGTAACAAATTTTTATTAGG
Product: formate acetyltransferase
Products: NA
Alternate protein names: Pyruvate formate-lyase 2 [H]
Number of amino acids: Translated: 787; Mature: 787
Protein sequence:
>787_residues MMTDRVKKLREESLKAVPRISMERTRIVTDVYKKYEGTVSIPVLRALVLKELMERKELCIYDGELIVGERGEAAAATPTY PELCCHTVEDFDIMDKREKISFKTTDEDKEIQEELIIPFWEKRSMRHKILEKMTPEWKACYEAGIFTEFMEQRGPGHTAG GDKYYKMGFLDIKEQIKEAISKLDYLNDDEALDKKEQLDAMDIACDAIMIYGKRYSEYAAKLAQKEADPVRKKELMEISE VCGWVPAHAPRTFREAIQMYWFVHLCVISELNPWDAFNPGRLDQHLYPFYKKEIEEGTLDREQARELLQCFWVKFNNQPA PPKVGITLKESGTYTDFANINSGGMKADGSDGVNDVSYLVLEVIDEMKLLQPSSNVQISKKTPQRFLKKACEVIRKGWGQ PSIFNADSVVQELVRAGKSIEDARCGGTSGCVEAGAFGKEAYILTGYFNLPKILEITLSNGVDSQTGKQLGIKTGDISTL KTYEDLLDAFKKQLKYFVDIKVNGNRVIERLYATLMPAPFLSVVTDDCIAKGKDYNAGGARYNTSYIQGVGIGTITDSLS AIKYQVFDEKNITMEELMEALRSNFEGHEDIYNLVKNKTPKYGNDDDYADEIMKEVFDAYYNEVNGRPNGRGGCYRIDML PTTCHVYFGSVINATPDGRKAHIPVSEGISPSKGADVNGPTGVIKSAAKMDHLRTGGTLLNQKFVPSVVQGEEGIDNMAN LVRAYFTMDGHHIQFNIVSKETLLKAQQNPDEYKDLIVRVAGYSDYFNNLDKVLQNEIIERTEQEFN
Sequences:
>Translated_787_residues MMTDRVKKLREESLKAVPRISMERTRIVTDVYKKYEGTVSIPVLRALVLKELMERKELCIYDGELIVGERGEAAAATPTY PELCCHTVEDFDIMDKREKISFKTTDEDKEIQEELIIPFWEKRSMRHKILEKMTPEWKACYEAGIFTEFMEQRGPGHTAG GDKYYKMGFLDIKEQIKEAISKLDYLNDDEALDKKEQLDAMDIACDAIMIYGKRYSEYAAKLAQKEADPVRKKELMEISE VCGWVPAHAPRTFREAIQMYWFVHLCVISELNPWDAFNPGRLDQHLYPFYKKEIEEGTLDREQARELLQCFWVKFNNQPA PPKVGITLKESGTYTDFANINSGGMKADGSDGVNDVSYLVLEVIDEMKLLQPSSNVQISKKTPQRFLKKACEVIRKGWGQ PSIFNADSVVQELVRAGKSIEDARCGGTSGCVEAGAFGKEAYILTGYFNLPKILEITLSNGVDSQTGKQLGIKTGDISTL KTYEDLLDAFKKQLKYFVDIKVNGNRVIERLYATLMPAPFLSVVTDDCIAKGKDYNAGGARYNTSYIQGVGIGTITDSLS AIKYQVFDEKNITMEELMEALRSNFEGHEDIYNLVKNKTPKYGNDDDYADEIMKEVFDAYYNEVNGRPNGRGGCYRIDML PTTCHVYFGSVINATPDGRKAHIPVSEGISPSKGADVNGPTGVIKSAAKMDHLRTGGTLLNQKFVPSVVQGEEGIDNMAN LVRAYFTMDGHHIQFNIVSKETLLKAQQNPDEYKDLIVRVAGYSDYFNNLDKVLQNEIIERTEQEFN >Mature_787_residues MMTDRVKKLREESLKAVPRISMERTRIVTDVYKKYEGTVSIPVLRALVLKELMERKELCIYDGELIVGERGEAAAATPTY PELCCHTVEDFDIMDKREKISFKTTDEDKEIQEELIIPFWEKRSMRHKILEKMTPEWKACYEAGIFTEFMEQRGPGHTAG GDKYYKMGFLDIKEQIKEAISKLDYLNDDEALDKKEQLDAMDIACDAIMIYGKRYSEYAAKLAQKEADPVRKKELMEISE VCGWVPAHAPRTFREAIQMYWFVHLCVISELNPWDAFNPGRLDQHLYPFYKKEIEEGTLDREQARELLQCFWVKFNNQPA PPKVGITLKESGTYTDFANINSGGMKADGSDGVNDVSYLVLEVIDEMKLLQPSSNVQISKKTPQRFLKKACEVIRKGWGQ PSIFNADSVVQELVRAGKSIEDARCGGTSGCVEAGAFGKEAYILTGYFNLPKILEITLSNGVDSQTGKQLGIKTGDISTL KTYEDLLDAFKKQLKYFVDIKVNGNRVIERLYATLMPAPFLSVVTDDCIAKGKDYNAGGARYNTSYIQGVGIGTITDSLS AIKYQVFDEKNITMEELMEALRSNFEGHEDIYNLVKNKTPKYGNDDDYADEIMKEVFDAYYNEVNGRPNGRGGCYRIDML PTTCHVYFGSVINATPDGRKAHIPVSEGISPSKGADVNGPTGVIKSAAKMDHLRTGGTLLNQKFVPSVVQGEEGIDNMAN LVRAYFTMDGHHIQFNIVSKETLLKAQQNPDEYKDLIVRVAGYSDYFNNLDKVLQNEIIERTEQEFN
Specific function: Glucose metabolism (nonoxidative conversion). [C]
COG id: COG1882
COG function: function code C; Pyruvate-formate lyase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 pyruvate formate lyase domain [H]
Homologues:
Organism=Escherichia coli, GI1790388, Length=801, Percent_Identity=33.832709113608, Blast_Score=428, Evalue=1e-121, Organism=Escherichia coli, GI1787044, Length=811, Percent_Identity=32.059186189889, Blast_Score=411, Evalue=1e-116, Organism=Escherichia coli, GI48994926, Length=699, Percent_Identity=23.3190271816881, Blast_Score=154, Evalue=2e-38, Organism=Escherichia coli, GI1787131, Length=680, Percent_Identity=22.5, Blast_Score=130, Evalue=4e-31,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001150 - InterPro: IPR019777 - InterPro: IPR004184 - InterPro: IPR010098 [H]
Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]
EC number: =2.3.1.54 [H]
Molecular weight: Translated: 88985; Mature: 88985
Theoretical pI: Translated: 5.10; Mature: 5.10
Prosite motif: PS51149 GLY_RADICAL_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.8 %Cys (Translated Protein) 2.9 %Met (Translated Protein) 4.7 %Cys+Met (Translated Protein) 1.8 %Cys (Mature Protein) 2.9 %Met (Mature Protein) 4.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MMTDRVKKLREESLKAVPRISMERTRIVTDVYKKYEGTVSIPVLRALVLKELMERKELCI CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEE YDGELIVGERGEAAAATPTYPELCCHTVEDFDIMDKREKISFKTTDEDKEIQEELIIPFW ECCEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHCCHHHCCCCCCCCHHHHHHHHCCCHH EKRSMRHKILEKMTPEWKACYEAGIFTEFMEQRGPGHTAGGDKYYKMGFLDIKEQIKEAI HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHH SKLDYLNDDEALDKKEQLDAMDIACDAIMIYGKRYSEYAAKLAQKEADPVRKKELMEISE HHHCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH VCGWVPAHAPRTFREAIQMYWFVHLCVISELNPWDAFNPGRLDQHLYPFYKKEIEEGTLD HHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC REQARELLQCFWVKFNNQPAPPKVGITLKESGTYTDFANINSGGMKADGSDGVNDVSYLV HHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH LEVIDEMKLLQPSSNVQISKKTPQRFLKKACEVIRKGWGQPSIFNADSVVQELVRAGKSI HHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCC EDARCGGTSGCVEAGAFGKEAYILTGYFNLPKILEITLSNGVDSQTGKQLGIKTGDISTL CHHCCCCCCCHHHCCCCCCCEEEEEECCCCCHHEEEEECCCCCCCCCCCCCCCCCCCHHH KTYEDLLDAFKKQLKYFVDIKVNGNRVIERLYATLMPAPFLSVVTDDCIAKGKDYNAGGA HHHHHHHHHHHHHHHEEEEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC RYNTSYIQGVGIGTITDSLSAIKYQVFDEKNITMEELMEALRSNFEGHEDIYNLVKNKTP EECCHHHCCCCCHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCC KYGNDDDYADEIMKEVFDAYYNEVNGRPNGRGGCYRIDMLPTTCHVYFGSVINATPDGRK CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCE AHIPVSEGISPSKGADVNGPTGVIKSAAKMDHLRTGGTLLNQKFVPSVVQGEEGIDNMAN EECCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCHHHCCCCCHHHHHH LVRAYFTMDGHHIQFNIVSKETLLKAQQNPDEYKDLIVRVAGYSDYFNNLDKVLQNEIIE HHHHHHCCCCCEEEEEEECHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH RTEQEFN HHHHHCC >Mature Secondary Structure MMTDRVKKLREESLKAVPRISMERTRIVTDVYKKYEGTVSIPVLRALVLKELMERKELCI CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEE YDGELIVGERGEAAAATPTYPELCCHTVEDFDIMDKREKISFKTTDEDKEIQEELIIPFW ECCEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHCCHHHCCCCCCCCHHHHHHHHCCCHH EKRSMRHKILEKMTPEWKACYEAGIFTEFMEQRGPGHTAGGDKYYKMGFLDIKEQIKEAI HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHH SKLDYLNDDEALDKKEQLDAMDIACDAIMIYGKRYSEYAAKLAQKEADPVRKKELMEISE HHHCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH VCGWVPAHAPRTFREAIQMYWFVHLCVISELNPWDAFNPGRLDQHLYPFYKKEIEEGTLD HHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC REQARELLQCFWVKFNNQPAPPKVGITLKESGTYTDFANINSGGMKADGSDGVNDVSYLV HHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH LEVIDEMKLLQPSSNVQISKKTPQRFLKKACEVIRKGWGQPSIFNADSVVQELVRAGKSI HHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCC EDARCGGTSGCVEAGAFGKEAYILTGYFNLPKILEITLSNGVDSQTGKQLGIKTGDISTL CHHCCCCCCCHHHCCCCCCCEEEEEECCCCCHHEEEEECCCCCCCCCCCCCCCCCCCHHH KTYEDLLDAFKKQLKYFVDIKVNGNRVIERLYATLMPAPFLSVVTDDCIAKGKDYNAGGA HHHHHHHHHHHHHHHEEEEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC RYNTSYIQGVGIGTITDSLSAIKYQVFDEKNITMEELMEALRSNFEGHEDIYNLVKNKTP EECCHHHCCCCCHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCC KYGNDDDYADEIMKEVFDAYYNEVNGRPNGRGGCYRIDMLPTTCHVYFGSVINATPDGRK CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCE AHIPVSEGISPSKGADVNGPTGVIKSAAKMDHLRTGGTLLNQKFVPSVVQGEEGIDNMAN EECCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCHHHCCCCCHHHHHH LVRAYFTMDGHHIQFNIVSKETLLKAQQNPDEYKDLIVRVAGYSDYFNNLDKVLQNEIIE HHHHHHCCCCCEEEEEEECHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH RTEQEFN HHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8265357; 9278503; 7773398 [H]