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The map label for this gene is folC [H]

Identifier: 226950592

GI number: 226950592

Start: 3605470

End: 3606759

Strand: Reverse

Name: folC [H]

Synonym: CLM_3575

Alternate gene names: 226950592

Gene position: 3606759-3605470 (Counterclockwise)

Preceding gene: 226950593

Following gene: 226950590

Centisome position: 86.8

GC content: 27.6

Gene sequence:

>1290_bases
ATGGATTATAAAGAAGCTAGAGAGTATATACAATCAAAGGCTAAGTTTGGTAGTAATTTAGGTCTAGAAAGAACAGAAAA
GCTTTTAGAGTTATTGGGAAACCCTCATAAGAGGTTAAGATGCATACATATAGCAGGTACAAATGGAAAAGGATCTACTA
CAGCTATGATTTCTACAGTATTAAAAGAGTCTGGCTATAAAGTTGGAATGTATACATCCCCTTATATAGAGGAGTTTGAA
GAAAGAATACAAATAAATGGCCATAATATAACTAAGGAAGATTTAGGTTATATTATAACTAAGGTAGCTAATATAGTGGA
AAAGGTAGAAAATATGGGATATGGAAATCCTACAGAATTTGAAATTATAACTGTTGCTATGTTTTATTATTTTTGTTTAA
AAGAAGTAGATTTTGCGGTAATAGAAGTAGGTTTAGGGGGACGGTTAGACTCTACTAATGTTTTAGAACCTATTTTAAGT
ATTATAACTTCTATAAGTTATGATCATATGAATATTTTAGGTGAAACTCTAGAAGAAATAACTTATGAAAAAGCAGGAAT
AATAAAAAAAGCTCCTGTTATAATGTATCCACAAAAGAAAGAAGTAGAAAAAAATATAGAAAAGGTGTGTAAGGAAAAAA
ATTGTGAGCTTATAAAAGTAGAAGATAATTTAATAAATATAGAAATAGAAATCATAGGAAAAAATATGGGGCAGCAAAGT
TTTAAATTAAAAACTAAAGAGAATACTTATAATATATGTTTATCCTTATTAGGAGAACATCAAATAAAAAACTGTATTAC
AGTTATATTAGCCTTAGAAAAGTTAATGAAATTAGGTATAAAAATAGAAAAAATACACATAATATCAGCCCTTAAAAAAG
TGAAATGGCCAGCTAGACTAGAAATAGTAAATAAAAATCCTCTAACAGTAATAGATGGAGCCCATAATATAGATGGCATA
GAAAGCTTAAAAAATAATGTGAGTAAATATTTTAAATATAATAAACTTATTTTAATATTAGGCATATTAAAAGATAAACA
AGTAGAAGACATGATAAAAACATTAGTACCACTAGCAGATAGAGTATTAACAGTAGCTCCTCATAATGATAGAGGGGAAA
GTTCAAAAGAATTAATGCACATTGTATTAAAACATAATGAAAGTTGTGAACATCTAGAGGACTATAAAGAATGTTATGAT
AAGGCAAAGTTTTACTGTGAAGATGGAGATATGATTTTAATTTGTGGGTCATTATACATGGTAGGCGATATGAGAAAATT
AATAAGGTAA

Upstream 100 bases:

>100_bases
TCTAGTCATTAGGTAATTTATTAAGGCTAAATAGAAATAATATAAAAGTAACAGAAGGGTTATGATTTACGTAAAAGATA
TACATAGGAAGTGATTATAT

Downstream 100 bases:

>100_bases
GGAAAGTGTTAGATGCTTTCCTTATTTTGTTTTTTTGCTTCTTGTAGAGCTTTAGACAATTGATCTGGACAGGAAGTATT
TTTAGAACCACAGGTTATAC

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MDYKEAREYIQSKAKFGSNLGLERTEKLLELLGNPHKRLRCIHIAGTNGKGSTTAMISTVLKESGYKVGMYTSPYIEEFE
ERIQINGHNITKEDLGYIITKVANIVEKVENMGYGNPTEFEIITVAMFYYFCLKEVDFAVIEVGLGGRLDSTNVLEPILS
IITSISYDHMNILGETLEEITYEKAGIIKKAPVIMYPQKKEVEKNIEKVCKEKNCELIKVEDNLINIEIEIIGKNMGQQS
FKLKTKENTYNICLSLLGEHQIKNCITVILALEKLMKLGIKIEKIHIISALKKVKWPARLEIVNKNPLTVIDGAHNIDGI
ESLKNNVSKYFKYNKLILILGILKDKQVEDMIKTLVPLADRVLTVAPHNDRGESSKELMHIVLKHNESCEHLEDYKECYD
KAKFYCEDGDMILICGSLYMVGDMRKLIR

Sequences:

>Translated_429_residues
MDYKEAREYIQSKAKFGSNLGLERTEKLLELLGNPHKRLRCIHIAGTNGKGSTTAMISTVLKESGYKVGMYTSPYIEEFE
ERIQINGHNITKEDLGYIITKVANIVEKVENMGYGNPTEFEIITVAMFYYFCLKEVDFAVIEVGLGGRLDSTNVLEPILS
IITSISYDHMNILGETLEEITYEKAGIIKKAPVIMYPQKKEVEKNIEKVCKEKNCELIKVEDNLINIEIEIIGKNMGQQS
FKLKTKENTYNICLSLLGEHQIKNCITVILALEKLMKLGIKIEKIHIISALKKVKWPARLEIVNKNPLTVIDGAHNIDGI
ESLKNNVSKYFKYNKLILILGILKDKQVEDMIKTLVPLADRVLTVAPHNDRGESSKELMHIVLKHNESCEHLEDYKECYD
KAKFYCEDGDMILICGSLYMVGDMRKLIR
>Mature_429_residues
MDYKEAREYIQSKAKFGSNLGLERTEKLLELLGNPHKRLRCIHIAGTNGKGSTTAMISTVLKESGYKVGMYTSPYIEEFE
ERIQINGHNITKEDLGYIITKVANIVEKVENMGYGNPTEFEIITVAMFYYFCLKEVDFAVIEVGLGGRLDSTNVLEPILS
IITSISYDHMNILGETLEEITYEKAGIIKKAPVIMYPQKKEVEKNIEKVCKEKNCELIKVEDNLINIEIEIIGKNMGQQS
FKLKTKENTYNICLSLLGEHQIKNCITVILALEKLMKLGIKIEKIHIISALKKVKWPARLEIVNKNPLTVIDGAHNIDGI
ESLKNNVSKYFKYNKLILILGILKDKQVEDMIKTLVPLADRVLTVAPHNDRGESSKELMHIVLKHNESCEHLEDYKECYD
KAKFYCEDGDMILICGSLYMVGDMRKLIR

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=303, Percent_Identity=27.0627062706271, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI66932984, Length=303, Percent_Identity=27.0627062706271, Blast_Score=133, Evalue=4e-31,
Organism=Escherichia coli, GI1788654, Length=414, Percent_Identity=26.8115942028986, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17553148, Length=443, Percent_Identity=26.1851015801354, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71984923, Length=443, Percent_Identity=26.1851015801354, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17553150, Length=443, Percent_Identity=26.1851015801354, Blast_Score=140, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323760, Length=436, Percent_Identity=32.1100917431193, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6324815, Length=324, Percent_Identity=31.4814814814815, Blast_Score=123, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6322718, Length=440, Percent_Identity=22.7272727272727, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24641571, Length=347, Percent_Identity=27.3775216138329, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24581568, Length=172, Percent_Identity=30.8139534883721, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 48876; Mature: 48876

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYKEAREYIQSKAKFGSNLGLERTEKLLELLGNPHKRLRCIHIAGTNGKGSTTAMISTV
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHEEEEEEECCCCCCCHHHHHHHH
LKESGYKVGMYTSPYIEEFEERIQINGHNITKEDLGYIITKVANIVEKVENMGYGNPTEF
HHHCCCEEECCCCHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
EIITVAMFYYFCLKEVDFAVIEVGLGGRLDSTNVLEPILSIITSISYDHMNILGETLEEI
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
TYEKAGIIKKAPVIMYPQKKEVEKNIEKVCKEKNCELIKVEDNLINIEIEIIGKNMGQQS
HHHHCCCEECCCEEECCCHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEEEEECCCCCCC
FKLKTKENTYNICLSLLGEHQIKNCITVILALEKLMKLGIKIEKIHIISALKKVKWPARL
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHCCCCCEE
EIVNKNPLTVIDGAHNIDGIESLKNNVSKYFKYNKLILILGILKDKQVEDMIKTLVPLAD
EEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEHHCCCHHHHHHHHHHHHHH
RVLTVAPHNDRGESSKELMHIVLKHNESCEHLEDYKECYDKAKFYCEDGDMILICGSLYM
HEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEECCCCEEEEECCHHH
VGDMRKLIR
HHHHHHHCC
>Mature Secondary Structure
MDYKEAREYIQSKAKFGSNLGLERTEKLLELLGNPHKRLRCIHIAGTNGKGSTTAMISTV
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHEEEEEEECCCCCCCHHHHHHHH
LKESGYKVGMYTSPYIEEFEERIQINGHNITKEDLGYIITKVANIVEKVENMGYGNPTEF
HHHCCCEEECCCCHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
EIITVAMFYYFCLKEVDFAVIEVGLGGRLDSTNVLEPILSIITSISYDHMNILGETLEEI
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
TYEKAGIIKKAPVIMYPQKKEVEKNIEKVCKEKNCELIKVEDNLINIEIEIIGKNMGQQS
HHHHCCCEECCCEEECCCHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEEEEECCCCCCC
FKLKTKENTYNICLSLLGEHQIKNCITVILALEKLMKLGIKIEKIHIISALKKVKWPARL
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHCCCCCEE
EIVNKNPLTVIDGAHNIDGIESLKNNVSKYFKYNKLILILGILKDKQVEDMIKTLVPLAD
EEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEHHCCCHHHHHHHHHHHHHH
RVLTVAPHNDRGESSKELMHIVLKHNESCEHLEDYKECYDKAKFYCEDGDMILICGSLYM
HEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEECCCCEEEEECCHHH
VGDMRKLIR
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]