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The map label for this gene is cheB [H]

Identifier: 226950162

GI number: 226950162

Start: 3114227

End: 3115291

Strand: Reverse

Name: cheB [H]

Synonym: CLM_3116

Alternate gene names: 226950162

Gene position: 3115291-3114227 (Counterclockwise)

Preceding gene: 226950163

Following gene: 226950161

Centisome position: 74.97

GC content: 30.89

Gene sequence:

>1065_bases
TTGAATAAAATTAAAGTTTTAGTAGTAGATGACTCGGCTTTAATGAGAAAAATAGTATCAGATATGATAAATGAAGAAAA
TGATTTAGAAGTAATAGATACAGCTAGAAATGGAGAAGATCTTTTAGAAAAATTAAAGAGGATAAAGCCAAATATAATAA
CATTAGATGTGGAAATGCCTAAAATGGATGGAATAGAAGCTTTAATAAATATTAAAAGGATTTATAAAGATATACCGGTT
ATTATGCTTAGTAGTTTATCTAAAAAAGGTGCAGGATTAACTATGGATTGTCTTCAAAAGGGAGCCTTTGATTTTGTACC
TAAGCCATCAGGGGCTATATCTTTAGATATAAATAAAGTAAAAGAAGAATTAGTAGAAAAAATAAGAGCTGCTACTAATG
CTATAAAAAATAATTATACTTCAGATATTTTAAAAACAGACGAAAAAATTAATGTTAAAAAAGTGGAAGAAAAAAGATAC
CCTATAAAAAATACTGTTTCTTCAAAAAAAGTAGAAGCTGTAGTTATAGGAGCTTCAACAGGAGGCCCTAAAGCATTATA
TTCAGTAATAACAGCTTTTCCAGAAAAAATAGATGTGCCAGTATTTGTAGTTCAACATATGCCAAAAGGATTTACCAAAG
CTTTTTCAGAAAGACTAAATGCTAATAGTAAATTAAAAGTTGTAGAAGGGCAAGATGGACAAACTATAGAGAAAGATACT
GTTTATGTAGCCCCGGGGGGCTTTCATATGGAAGTAGGAAAGGATAGAAAAATACATCTAAACATAGAACCAACCCTATG
GGGAGTGAGACCAGCTGTAGATAAGTTATTTAAATCAGCATCTGAAGTTTATGGCAGCAACTTAATAAGTGTTGTATTAA
CTGGTATGGGAAGAGATGGCGCAGATGGTACGGCTTATATAAAAGATAGAGGAGGCATAACCATATCAGAAGATAAATCT
ACCTGTACTATATACGGTATGCCAAAAGCAACTTACGAAACAGGAAAGGTGGATTTTGTAGTACCACTTTATAATGTTAA
AGATACAATACTGAAAAATATTTAA

Upstream 100 bases:

>100_bases
GGAAGAACTATGATATTTGATACCTCCACAGGAAAAGTATATATAAAGACTGTAGGCTTAGGAACTAAAGAAATATAGAT
AAGTTATTGGAGTGATTCAT

Downstream 100 bases:

>100_bases
TTTTGGAGGTAATTATGGATTTAGAATATTTCGAAGAGTGGGTATTAAAGGATTTTGGAATAAATTTAAAAGCATATAAA
CAAAATCAGCTTCAAAGAAG

Product: protein-glutamate methylesterase CheB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MNKIKVLVVDDSALMRKIVSDMINEENDLEVIDTARNGEDLLEKLKRIKPNIITLDVEMPKMDGIEALINIKRIYKDIPV
IMLSSLSKKGAGLTMDCLQKGAFDFVPKPSGAISLDINKVKEELVEKIRAATNAIKNNYTSDILKTDEKINVKKVEEKRY
PIKNTVSSKKVEAVVIGASTGGPKALYSVITAFPEKIDVPVFVVQHMPKGFTKAFSERLNANSKLKVVEGQDGQTIEKDT
VYVAPGGFHMEVGKDRKIHLNIEPTLWGVRPAVDKLFKSASEVYGSNLISVVLTGMGRDGADGTAYIKDRGGITISEDKS
TCTIYGMPKATYETGKVDFVVPLYNVKDTILKNI

Sequences:

>Translated_354_residues
MNKIKVLVVDDSALMRKIVSDMINEENDLEVIDTARNGEDLLEKLKRIKPNIITLDVEMPKMDGIEALINIKRIYKDIPV
IMLSSLSKKGAGLTMDCLQKGAFDFVPKPSGAISLDINKVKEELVEKIRAATNAIKNNYTSDILKTDEKINVKKVEEKRY
PIKNTVSSKKVEAVVIGASTGGPKALYSVITAFPEKIDVPVFVVQHMPKGFTKAFSERLNANSKLKVVEGQDGQTIEKDT
VYVAPGGFHMEVGKDRKIHLNIEPTLWGVRPAVDKLFKSASEVYGSNLISVVLTGMGRDGADGTAYIKDRGGITISEDKS
TCTIYGMPKATYETGKVDFVVPLYNVKDTILKNI
>Mature_354_residues
MNKIKVLVVDDSALMRKIVSDMINEENDLEVIDTARNGEDLLEKLKRIKPNIITLDVEMPKMDGIEALINIKRIYKDIPV
IMLSSLSKKGAGLTMDCLQKGAFDFVPKPSGAISLDINKVKEELVEKIRAATNAIKNNYTSDILKTDEKINVKKVEEKRY
PIKNTVSSKKVEAVVIGASTGGPKALYSVITAFPEKIDVPVFVVQHMPKGFTKAFSERLNANSKLKVVEGQDGQTIEKDT
VYVAPGGFHMEVGKDRKIHLNIEPTLWGVRPAVDKLFKSASEVYGSNLISVVLTGMGRDGADGTAYIKDRGGITISEDKS
TCTIYGMPKATYETGKVDFVVPLYNVKDTILKNI

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR [H]

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=358, Percent_Identity=37.1508379888268, Blast_Score=234, Evalue=8e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789 [H]

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg [H]

EC number: =3.1.1.61 [H]

Molecular weight: Translated: 39010; Mature: 39010

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50122 CHEB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIKVLVVDDSALMRKIVSDMINEENDLEVIDTARNGEDLLEKLKRIKPNIITLDVEMP
CCCEEEEEECCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEEEECC
KMDGIEALINIKRIYKDIPVIMLSSLSKKGAGLTMDCLQKGAFDFVPKPSGAISLDINKV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCEEEEEHHHH
KEELVEKIRAATNAIKNNYTSDILKTDEKINVKKVEEKRYPIKNTVSSKKVEAVVIGAST
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEHHHHCCCCHHCCCCCCEEEEEEECCC
GGPKALYSVITAFPEKIDVPVFVVQHMPKGFTKAFSERLNANSKLKVVEGQDGQTIEKDT
CCHHHHHHHHHHCHHHCCCCEEEEHHCCCHHHHHHHHHHCCCCEEEEEECCCCCEEECCE
VYVAPGGFHMEVGKDRKIHLNIEPTLWGVRPAVDKLFKSASEVYGSNLISVVLTGMGRDG
EEECCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHEEHHHCCCCCCC
ADGTAYIKDRGGITISEDKSTCTIYGMPKATYETGKVDFVVPLYNVKDTILKNI
CCCEEEEECCCCEEEECCCCEEEEEECCCCCCCCCCEEEEEEECCCHHHHHHCC
>Mature Secondary Structure
MNKIKVLVVDDSALMRKIVSDMINEENDLEVIDTARNGEDLLEKLKRIKPNIITLDVEMP
CCCEEEEEECCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEEEECC
KMDGIEALINIKRIYKDIPVIMLSSLSKKGAGLTMDCLQKGAFDFVPKPSGAISLDINKV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCEEEEEHHHH
KEELVEKIRAATNAIKNNYTSDILKTDEKINVKKVEEKRYPIKNTVSSKKVEAVVIGAST
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEHHHHCCCCHHCCCCCCEEEEEEECCC
GGPKALYSVITAFPEKIDVPVFVVQHMPKGFTKAFSERLNANSKLKVVEGQDGQTIEKDT
CCHHHHHHHHHHCHHHCCCCEEEEHHCCCHHHHHHHHHHCCCCEEEEEECCCCCEEECCE
VYVAPGGFHMEVGKDRKIHLNIEPTLWGVRPAVDKLFKSASEVYGSNLISVVLTGMGRDG
EEECCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHEEHHHCCCCCCC
ADGTAYIKDRGGITISEDKSTCTIYGMPKATYETGKVDFVVPLYNVKDTILKNI
CCCEEEEECCCCEEEECCCCEEEEEECCCCCCCCCCEEEEEEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]