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The map label for this gene is rffA [H]

Identifier: 226950127

GI number: 226950127

Start: 3079789

End: 3080919

Strand: Reverse

Name: rffA [H]

Synonym: CLM_3081

Alternate gene names: 226950127

Gene position: 3080919-3079789 (Counterclockwise)

Preceding gene: 226950128

Following gene: 226950126

Centisome position: 74.14

GC content: 27.14

Gene sequence:

>1131_bases
ATGATACCATTTAATAAAGTATATACAACTAACAATGAAATAGAGTATATAAAAGATGCAATAAATAGAAAACATGTATC
TGGTGATGGATATTATACTAAAAAAGTTGCTAATTTTATGGAACAAAAATTTAATACAAAGAAAGCTCTTATGACTACTT
CTTGCAGTTCCGCTCTTGATATAGCAGCAATTCTTTTAAATTTAAAAGAAGGCGATGAAGTTATTATGCCTTCATATACT
TTTGTATCTACGGCAAATTCAGTAGTACTTAGGGGGGCAAAACCAGTATTTGCAGATATAAATAAAGAAACATTAAATAT
AGATCCAGAAGATATAAAAAGAAAAATAACTAAAAATACAAAGGCTATTTTCGTTGTTCATTATGCCGGAGTAGCTTGTG
ATATGGATAAAATAATGAAAATAGCTAAAAAGAATAATTTATTAGTAGTTGAAGATGCAGCTCAAGGAGTTAATGCAAAA
TATAAGGGGAAATATTTAGGAACCATAGGAGATATAGGTTGCTATAGTTTTCATGAAACTAAAAATTATTCTTCAGGAGA
AGGTGGAGCTATACTTATAAATAAAGATGAAAAATTATGTAGAAAATCTGAAATAGTAAGAGAGAAAGGAACTAACCGAA
GTCAATTTTTTAGAGGAGAAGTGGATAAATATACTTGGATAGATAAAGGTTCAAGTTATTTGCCATCAGATATATTAGTA
GCTTTTTTATGGGCACAATTTCAGAAAATGGATGAAATTCAGCAAAAAAGAAAAAATATTTATGAAAATTATTATAAAGG
ATTAAAAAAACTACAAGACAAGGGTGTTTTGACATTACCTATTATACCAAAAGAATGTGAAAGTAATTATCATATATTTC
ATATACTTTTGAATTCAGAAAAAGAAAGAAATTCTTTAATGTATAAATTAAAAGAGAAAAACATAGGAGCAGTATTTCAT
TATGTACCATTACATACCTCATCTATGGGAGAAAAATTAGGATATAAGTTAGGTGACTTACCTAATACGGAAAATCTAAG
TTGGAGATTGTTAAGATTACCCATGTATGTGGAGTTAAGTTTAGAGGATCAAAACTACATAATTAATGAAATAAATAAAA
TATTTTTATAA

Upstream 100 bases:

>100_bases
CATTTTGTTACTGTTTTAGATTTGATATTTAATTGTGGTTCCGATGCCCCATATTATATATGGGGATGGAGAGAAGATAA
TAACAGAGAGGAAATGGAAT

Downstream 100 bases:

>100_bases
GTTTTTTGGTGTTTTGATTTAAATTTAGGAGGATTTATGTATGGAAAAAATAATATTATTTGGTGCTTCAAAAATGGGTG
AGGCTGCTTGTGTGTTATTA

Product: TDP-4-oxo-6-deoxy-D-glucose transaminase

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MIPFNKVYTTNNEIEYIKDAINRKHVSGDGYYTKKVANFMEQKFNTKKALMTTSCSSALDIAAILLNLKEGDEVIMPSYT
FVSTANSVVLRGAKPVFADINKETLNIDPEDIKRKITKNTKAIFVVHYAGVACDMDKIMKIAKKNNLLVVEDAAQGVNAK
YKGKYLGTIGDIGCYSFHETKNYSSGEGGAILINKDEKLCRKSEIVREKGTNRSQFFRGEVDKYTWIDKGSSYLPSDILV
AFLWAQFQKMDEIQQKRKNIYENYYKGLKKLQDKGVLTLPIIPKECESNYHIFHILLNSEKERNSLMYKLKEKNIGAVFH
YVPLHTSSMGEKLGYKLGDLPNTENLSWRLLRLPMYVELSLEDQNYIINEINKIFL

Sequences:

>Translated_376_residues
MIPFNKVYTTNNEIEYIKDAINRKHVSGDGYYTKKVANFMEQKFNTKKALMTTSCSSALDIAAILLNLKEGDEVIMPSYT
FVSTANSVVLRGAKPVFADINKETLNIDPEDIKRKITKNTKAIFVVHYAGVACDMDKIMKIAKKNNLLVVEDAAQGVNAK
YKGKYLGTIGDIGCYSFHETKNYSSGEGGAILINKDEKLCRKSEIVREKGTNRSQFFRGEVDKYTWIDKGSSYLPSDILV
AFLWAQFQKMDEIQQKRKNIYENYYKGLKKLQDKGVLTLPIIPKECESNYHIFHILLNSEKERNSLMYKLKEKNIGAVFH
YVPLHTSSMGEKLGYKLGDLPNTENLSWRLLRLPMYVELSLEDQNYIINEINKIFL
>Mature_376_residues
MIPFNKVYTTNNEIEYIKDAINRKHVSGDGYYTKKVANFMEQKFNTKKALMTTSCSSALDIAAILLNLKEGDEVIMPSYT
FVSTANSVVLRGAKPVFADINKETLNIDPEDIKRKITKNTKAIFVVHYAGVACDMDKIMKIAKKNNLLVVEDAAQGVNAK
YKGKYLGTIGDIGCYSFHETKNYSSGEGGAILINKDEKLCRKSEIVREKGTNRSQFFRGEVDKYTWIDKGSSYLPSDILV
AFLWAQFQKMDEIQQKRKNIYENYYKGLKKLQDKGVLTLPIIPKECESNYHIFHILLNSEKERNSLMYKLKEKNIGAVFH
YVPLHTSSMGEKLGYKLGDLPNTENLSWRLLRLPMYVELSLEDQNYIINEINKIFL

Specific function: Involved in ECA elongation [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI2367285, Length=376, Percent_Identity=52.9255319148936, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI145693159, Length=384, Percent_Identity=30.9895833333333, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR012749
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 43035; Mature: 43035

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPFNKVYTTNNEIEYIKDAINRKHVSGDGYYTKKVANFMEQKFNTKKALMTTSCSSALD
CCCCCCEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHEECCCHHHHH
IAAILLNLKEGDEVIMPSYTFVSTANSVVLRGAKPVFADINKETLNIDPEDIKRKITKNT
HHHHHHCCCCCCEEEECCCHHHHCCCCEEEECCCCCEECCCCCEECCCHHHHHHHHHCCC
KAIFVVHYAGVACDMDKIMKIAKKNNLLVVEDAAQGVNAKYKGKYLGTIGDIGCYSFHET
CEEEEEEECCCCCCHHHHHHHHHCCCEEEEECCCCCCCCEECCCEEECCCCCCCEEECCC
KNYSSGEGGAILINKDEKLCRKSEIVREKGTNRSQFFRGEVDKYTWIDKGSSYLPSDILV
CCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEECCCCCCCCHHHHH
AFLWAQFQKMDEIQQKRKNIYENYYKGLKKLQDKGVLTLPIIPKECESNYHIFHILLNSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHCCCCEEEEEEECCC
KERNSLMYKLKEKNIGAVFHYVPLHTSSMGEKLGYKLGDLPNTENLSWRLLRLPMYVELS
CHHHHHEEEEECCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCEEEEEEECEEEEEE
LEDQNYIINEINKIFL
ECCCCHHHHHHHHHCC
>Mature Secondary Structure
MIPFNKVYTTNNEIEYIKDAINRKHVSGDGYYTKKVANFMEQKFNTKKALMTTSCSSALD
CCCCCCEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHEECCCHHHHH
IAAILLNLKEGDEVIMPSYTFVSTANSVVLRGAKPVFADINKETLNIDPEDIKRKITKNT
HHHHHHCCCCCCEEEECCCHHHHCCCCEEEECCCCCEECCCCCEECCCHHHHHHHHHCCC
KAIFVVHYAGVACDMDKIMKIAKKNNLLVVEDAAQGVNAKYKGKYLGTIGDIGCYSFHET
CEEEEEEECCCCCCHHHHHHHHHCCCEEEEECCCCCCCCEECCCEEECCCCCCCEEECCC
KNYSSGEGGAILINKDEKLCRKSEIVREKGTNRSQFFRGEVDKYTWIDKGSSYLPSDILV
CCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEECCCCCCCCHHHHH
AFLWAQFQKMDEIQQKRKNIYENYYKGLKKLQDKGVLTLPIIPKECESNYHIFHILLNSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHCCCCEEEEEEECCC
KERNSLMYKLKEKNIGAVFHYVPLHTSSMGEKLGYKLGDLPNTENLSWRLLRLPMYVELS
CHHHHHEEEEECCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCEEEEEEECEEEEEE
LEDQNYIINEINKIFL
ECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 8366065 [H]