| Definition | Clostridium botulinum A2 str. Kyoto chromosome, complete genome. |
|---|---|
| Accession | NC_012563 |
| Length | 4,155,278 |
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The map label for this gene is pps [H]
Identifier: 226949937
GI number: 226949937
Start: 2899211
End: 2901688
Strand: Reverse
Name: pps [H]
Synonym: CLM_2889
Alternate gene names: 226949937
Gene position: 2901688-2899211 (Counterclockwise)
Preceding gene: 226949938
Following gene: 226949936
Centisome position: 69.83
GC content: 28.17
Gene sequence:
>2478_bases ATGGACACTAAAAATATTATTGGTATAAAAGATGCACGGGAAAATCAAATTGATATTGTAGGAGGAAAAGGGGCTAATTT AGGACTAATGATTTCATGTGGTATTCCTGTTCCAGATGGCTTTATTGTAACGGCTAATGCATATAAAAATTTTTTAAAAA GTAATGGTATATTAGAAATAATAGAACAAAAAATTAGTAATATAGATAAAGAAACTTTATCTATAAAGGATAAAACTGAA GAGATAAGAAATTTAATTTTAAAAGCTGAGTTCCCAAAGGACTTAAAAGAAGATATATTGAGTAGATTTAATAAATTTAA GAAACCTATTCACTTAGCAGTAAGATCTTCGGCAACAGCCGAAGATTTACCGGAAGCAAGTTTTGCAGGGCAACAAGAGA CATATTTGAATATTATGAACAAGGAAGATTTATTTGTATCAATAAAAAAGTGCTTTTCTTCTCTTTGGAGCATTAGGGCA TTTTCATATAGAACTAATAATGGATATGATCATTTGAATGTTGGTATAGCAGTTGTTATTCAAGAAATGATAGAATCTGA TATTTCTGGAGTTATGTTTACATCTAACCCAATAACTGCTGAAAAGGAAATAATGATAGATGCATCCTATAATTTGGGAG AAGCCATAGTGTCAGGAAAAGTTACGCCAGATAATTATGTTCTTGATAAAAATGGAGAAGAAATAGCTTTCACTCTTGGA AGTAAGGAAATTTCAGTTGTATATTCAGATAAAGGAACTGTGGTTGTAAATAATTCTTCTGATATAAGAGAAAGAAGATG TCTTCATAATGAAAATTTAAGAGAATTATTTGATATGGCTTTAAAGATAGAAGGTTTATATAAAAAACCTATGGATATAG AATGGGCAATTAAAAATAAAAAAGTATATATTCTTCAGGCACGCTCAATTACAACTATAAAGAAAAAACAGAATAAAAAT ACCAGAAAGCAGTACTCACAAAAAGAAAAAAAGTATTTAAATAATATGATAGAACATTTTCCAGTTGCATGTTATCCACT AGATTATGAAATTGCAATGATACTTCAAAATATGAAATTTGAACTTTTTAAGGATGTAGGACTGAAAATAGAAAGTCCTA TATATATGGATAATATGGGTATAATCCATATGAATAAAATTAGGTGGAAAGTAAATCCAAAAATTTTACGCTTTTTTAAA GTTTATTATGAATATAAAAATAATGAGAAGAATGTTGAATATGGAGAAAAGATTTTAGATTCTTGTAAAAAAGAACTCAA AGTAATAGAACAAAATATAAATGAAAATAGTTATAAATTTTCTCTTCGTGATATAGATAAATATATGAGTGATATTATTT CAATTCATGAAAGAATTTCTTTTGCAAGGTTTAGATATTTTCTTTTCCCATCTGTTATTATTGGAGAAAAACTTGATAAA GAACTTAAATCTATAAATCCAAATTATACAGAGTATGATCTCTTATCAGATTTAGATTATGTAACAGTAGATATGAATAA GGATATTGATATTTTATCAAAAACTTTATCAAGAAATGAAGAACTAATAAAGGATTTAATAAAAGGGGCAAGCTATGCAT TTATAAAAGAAAAATATGATTATGATTCAATAGAAATAAAAGAGTTTTTTAATAAATATGGTTGGAAGTCTAATTATTGT TGTATTCCATTCTCTTCATCTTCTTGGAATGAGGAACCAAATAGATTTATAAAACTTCTTAAAATAACCATTTCAGAGAA TAAAGAATATGAAGAAAATAAATCTGGAAAGTACATGGGTATATTGAAAGAGTTGGAGGAAAGTAAAGGTAAAAAAAGAT TTAAGAAAACAATGGGAGATATTACTTATTATAGACTAGCTCATGTTAGACGTGAGGAAAGTCAATATCTTTGGGAAACA GGATTTGGATTATTGAGGTTGCTTTTAAAACAAATAGAAGATTTGTTAAATACAAAGAAAGAAGATTTGCTATATTTGTC TTTTCAAGAGTTAAGAAAAGTAATAGCTATTGGTAAGTTAGAGCATGAATATAGAGAATTAATTTCATATAGGAAAAATC ACCGTAAGGAGGCTGAAGAACTTTGGGAGCAGATGGCATTAGATGCCTTTTATGATAATGAGAAGAATATTTTCAAGGGA ATAAGCGGAAGTTCAGGTGTAGTGAGAGGTAGAGTTTGTATTATAAAAAATCATACTGAATTTGATAAACTGAAAGAAGA TAATATTCTTATATGTCCATATACTGATCCTGAGTGGACTCCTCTTTTTAAAATAGCAAAAGGAGTTGTTTCAGATACAG GTGGAGCTTTATCGCACGCAGCTATTGTTGCACGTGAATATAATATCCCAGCGGTATTGGGTGTTGGAAATGCCACAGTA AATTTATCGGATGGGGAATATATATTATTAGACGGAAATAATGGTATTGTAAAGCGATTATCAGAGGAAATAATATGA
Upstream 100 bases:
>100_bases GATGCTACAGTAGATCTTTATAAATCAATATCTCTGAATGAAATAGTTGAGAATATTGAAAAGACTAAAATTGATTTAGA AGATATAGTGGGGTGAAAAA
Downstream 100 bases:
>100_bases CAAAGAATATAGAAGACAAACAGGGAAAATTTGAAGAACTTACTAAGACGCCAATTCCCAAATTAATATTGAAAATGGCT ATACCGTCTACAATGGCAAT
Product: putative phosphoenolpyruvate synthase
Products: AMP; phosphoenolpyruvate; phosphate
Alternate protein names: Putative PEP synthase; Pyruvate, water dikinase [H]
Number of amino acids: Translated: 825; Mature: 825
Protein sequence:
>825_residues MDTKNIIGIKDARENQIDIVGGKGANLGLMISCGIPVPDGFIVTANAYKNFLKSNGILEIIEQKISNIDKETLSIKDKTE EIRNLILKAEFPKDLKEDILSRFNKFKKPIHLAVRSSATAEDLPEASFAGQQETYLNIMNKEDLFVSIKKCFSSLWSIRA FSYRTNNGYDHLNVGIAVVIQEMIESDISGVMFTSNPITAEKEIMIDASYNLGEAIVSGKVTPDNYVLDKNGEEIAFTLG SKEISVVYSDKGTVVVNNSSDIRERRCLHNENLRELFDMALKIEGLYKKPMDIEWAIKNKKVYILQARSITTIKKKQNKN TRKQYSQKEKKYLNNMIEHFPVACYPLDYEIAMILQNMKFELFKDVGLKIESPIYMDNMGIIHMNKIRWKVNPKILRFFK VYYEYKNNEKNVEYGEKILDSCKKELKVIEQNINENSYKFSLRDIDKYMSDIISIHERISFARFRYFLFPSVIIGEKLDK ELKSINPNYTEYDLLSDLDYVTVDMNKDIDILSKTLSRNEELIKDLIKGASYAFIKEKYDYDSIEIKEFFNKYGWKSNYC CIPFSSSSWNEEPNRFIKLLKITISENKEYEENKSGKYMGILKELEESKGKKRFKKTMGDITYYRLAHVRREESQYLWET GFGLLRLLLKQIEDLLNTKKEDLLYLSFQELRKVIAIGKLEHEYRELISYRKNHRKEAEELWEQMALDAFYDNEKNIFKG ISGSSGVVRGRVCIIKNHTEFDKLKEDNILICPYTDPEWTPLFKIAKGVVSDTGGALSHAAIVAREYNIPAVLGVGNATV NLSDGEYILLDGNNGIVKRLSEEII
Sequences:
>Translated_825_residues MDTKNIIGIKDARENQIDIVGGKGANLGLMISCGIPVPDGFIVTANAYKNFLKSNGILEIIEQKISNIDKETLSIKDKTE EIRNLILKAEFPKDLKEDILSRFNKFKKPIHLAVRSSATAEDLPEASFAGQQETYLNIMNKEDLFVSIKKCFSSLWSIRA FSYRTNNGYDHLNVGIAVVIQEMIESDISGVMFTSNPITAEKEIMIDASYNLGEAIVSGKVTPDNYVLDKNGEEIAFTLG SKEISVVYSDKGTVVVNNSSDIRERRCLHNENLRELFDMALKIEGLYKKPMDIEWAIKNKKVYILQARSITTIKKKQNKN TRKQYSQKEKKYLNNMIEHFPVACYPLDYEIAMILQNMKFELFKDVGLKIESPIYMDNMGIIHMNKIRWKVNPKILRFFK VYYEYKNNEKNVEYGEKILDSCKKELKVIEQNINENSYKFSLRDIDKYMSDIISIHERISFARFRYFLFPSVIIGEKLDK ELKSINPNYTEYDLLSDLDYVTVDMNKDIDILSKTLSRNEELIKDLIKGASYAFIKEKYDYDSIEIKEFFNKYGWKSNYC CIPFSSSSWNEEPNRFIKLLKITISENKEYEENKSGKYMGILKELEESKGKKRFKKTMGDITYYRLAHVRREESQYLWET GFGLLRLLLKQIEDLLNTKKEDLLYLSFQELRKVIAIGKLEHEYRELISYRKNHRKEAEELWEQMALDAFYDNEKNIFKG ISGSSGVVRGRVCIIKNHTEFDKLKEDNILICPYTDPEWTPLFKIAKGVVSDTGGALSHAAIVAREYNIPAVLGVGNATV NLSDGEYILLDGNNGIVKRLSEEII >Mature_825_residues MDTKNIIGIKDARENQIDIVGGKGANLGLMISCGIPVPDGFIVTANAYKNFLKSNGILEIIEQKISNIDKETLSIKDKTE EIRNLILKAEFPKDLKEDILSRFNKFKKPIHLAVRSSATAEDLPEASFAGQQETYLNIMNKEDLFVSIKKCFSSLWSIRA FSYRTNNGYDHLNVGIAVVIQEMIESDISGVMFTSNPITAEKEIMIDASYNLGEAIVSGKVTPDNYVLDKNGEEIAFTLG SKEISVVYSDKGTVVVNNSSDIRERRCLHNENLRELFDMALKIEGLYKKPMDIEWAIKNKKVYILQARSITTIKKKQNKN TRKQYSQKEKKYLNNMIEHFPVACYPLDYEIAMILQNMKFELFKDVGLKIESPIYMDNMGIIHMNKIRWKVNPKILRFFK VYYEYKNNEKNVEYGEKILDSCKKELKVIEQNINENSYKFSLRDIDKYMSDIISIHERISFARFRYFLFPSVIIGEKLDK ELKSINPNYTEYDLLSDLDYVTVDMNKDIDILSKTLSRNEELIKDLIKGASYAFIKEKYDYDSIEIKEFFNKYGWKSNYC CIPFSSSSWNEEPNRFIKLLKITISENKEYEENKSGKYMGILKELEESKGKKRFKKTMGDITYYRLAHVRREESQYLWET GFGLLRLLLKQIEDLLNTKKEDLLYLSFQELRKVIAIGKLEHEYRELISYRKNHRKEAEELWEQMALDAFYDNEKNIFKG ISGSSGVVRGRVCIIKNHTEFDKLKEDNILICPYTDPEWTPLFKIAKGVVSDTGGALSHAAIVAREYNIPAVLGVGNATV NLSDGEYILLDGNNGIVKRLSEEII
Specific function: Might catalyze the phosphorylation of pyruvate to phosphoenolpyruvate (Potential) [H]
COG id: COG0574
COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the PEP-utilizing enzyme family [H]
Homologues:
Organism=Escherichia coli, GI1787994, Length=356, Percent_Identity=35.3932584269663, Blast_Score=196, Evalue=7e-51, Organism=Caenorhabditis elegans, GI17564524, Length=896, Percent_Identity=26.3392857142857, Blast_Score=197, Evalue=2e-50,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013815 - InterPro: IPR013816 - InterPro: IPR008279 - InterPro: IPR002192 [H]
Pfam domain/function: PF00391 PEP-utilizers; PF01326 PPDK_N [H]
EC number: 2.7.9.2
Molecular weight: Translated: 95418; Mature: 95418
Theoretical pI: Translated: 7.50; Mature: 7.50
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 3.4 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 2.3 %Met (Mature Protein) 3.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MDTKNIIGIKDARENQIDIVGGKGANLGLMISCGIPVPDGFIVTANAYKNFLKSNGILEI CCCCCEEECCCCCCCCEEEEECCCCCEEEEEECCCCCCCCEEEECHHHHHHHHCCCHHHH IEQKISNIDKETLSIKDKTEEIRNLILKAEFPKDLKEDILSRFNKFKKPIHLAVRSSATA HHHHHHCCCHHHEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCH EDLPEASFAGQQETYLNIMNKEDLFVSIKKCFSSLWSIRAFSYRTNNGYDHLNVGIAVVI HHCCCCCCCCCCHHHHEECCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHH QEMIESDISGVMFTSNPITAEKEIMIDASYNLGEAIVSGKVTPDNYVLDKNGEEIAFTLG HHHHHHCCCCEEEECCCCCCCCEEEEECCCCCCHHHHCCCCCCCCEEECCCCCEEEEEEC SKEISVVYSDKGTVVVNNSSDIRERRCLHNENLRELFDMALKIEGLYKKPMDIEWAIKNK CCEEEEEEECCCEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECC KVYILQARSITTIKKKQNKNTRKQYSQKEKKYLNNMIEHFPVACYPLDYEIAMILQNMKF EEEEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHH ELFKDVGLKIESPIYMDNMGIIHMNKIRWKVNPKILRFFKVYYEYKNNEKNVEYGEKILD HHHHHCCCEECCCEEECCCCEEEEEEEEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHH SCKKELKVIEQNINENSYKFSLRDIDKYMSDIISIHERISFARFRYFLFPSVIIGEKLDK HHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH ELKSINPNYTEYDLLSDLDYVTVDMNKDIDILSKTLSRNEELIKDLIKGASYAFIKEKYD HHHHCCCCCCHHHHHCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHHCCCCEEEEHHCCC YDSIEIKEFFNKYGWKSNYCCIPFSSSSWNEEPNRFIKLLKITISENKEYEENKSGKYMG CCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHEEEECCCCCCCCCCCCCHHH ILKELEESKGKKRFKKTMGDITYYRLAHVRREESQYLWETGFGLLRLLLKQIEDLLNTKK HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH EDLLYLSFQELRKVIAIGKLEHEYRELISYRKNHRKEAEELWEQMALDAFYDNEKNIFKG HHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC ISGSSGVVRGRVCIIKNHTEFDKLKEDNILICPYTDPEWTPLFKIAKGVVSDTGGALSHA CCCCCCEEEEEEEEEECCCHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHH AIVAREYNIPAVLGVGNATVNLSDGEYILLDGNNGIVKRLSEEII HHEEEECCCCEEEECCCEEEEECCCCEEEEECCCHHHHHHHHHCC >Mature Secondary Structure MDTKNIIGIKDARENQIDIVGGKGANLGLMISCGIPVPDGFIVTANAYKNFLKSNGILEI CCCCCEEECCCCCCCCEEEEECCCCCEEEEEECCCCCCCCEEEECHHHHHHHHCCCHHHH IEQKISNIDKETLSIKDKTEEIRNLILKAEFPKDLKEDILSRFNKFKKPIHLAVRSSATA HHHHHHCCCHHHEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCH EDLPEASFAGQQETYLNIMNKEDLFVSIKKCFSSLWSIRAFSYRTNNGYDHLNVGIAVVI HHCCCCCCCCCCHHHHEECCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHH QEMIESDISGVMFTSNPITAEKEIMIDASYNLGEAIVSGKVTPDNYVLDKNGEEIAFTLG HHHHHHCCCCEEEECCCCCCCCEEEEECCCCCCHHHHCCCCCCCCEEECCCCCEEEEEEC SKEISVVYSDKGTVVVNNSSDIRERRCLHNENLRELFDMALKIEGLYKKPMDIEWAIKNK CCEEEEEEECCCEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECC KVYILQARSITTIKKKQNKNTRKQYSQKEKKYLNNMIEHFPVACYPLDYEIAMILQNMKF EEEEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHH ELFKDVGLKIESPIYMDNMGIIHMNKIRWKVNPKILRFFKVYYEYKNNEKNVEYGEKILD HHHHHCCCEECCCEEECCCCEEEEEEEEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHH SCKKELKVIEQNINENSYKFSLRDIDKYMSDIISIHERISFARFRYFLFPSVIIGEKLDK HHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH ELKSINPNYTEYDLLSDLDYVTVDMNKDIDILSKTLSRNEELIKDLIKGASYAFIKEKYD HHHHCCCCCCHHHHHCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHHCCCCEEEEHHCCC YDSIEIKEFFNKYGWKSNYCCIPFSSSSWNEEPNRFIKLLKITISENKEYEENKSGKYMG CCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHEEEECCCCCCCCCCCCCHHH ILKELEESKGKKRFKKTMGDITYYRLAHVRREESQYLWETGFGLLRLLLKQIEDLLNTKK HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH EDLLYLSFQELRKVIAIGKLEHEYRELISYRKNHRKEAEELWEQMALDAFYDNEKNIFKG HHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC ISGSSGVVRGRVCIIKNHTEFDKLKEDNILICPYTDPEWTPLFKIAKGVVSDTGGALSHA CCCCCCEEEEEEEEEECCCHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHH AIVAREYNIPAVLGVGNATVNLSDGEYILLDGNNGIVKRLSEEII HHEEEECCCCEEEECCCEEEEECCCCEEEEECCCHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: Mg2+; Mn2+ [C]
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): 10.5 {phosphate}} 0.083 {pyruvate}} 0.028 {ATP}} [C]
Substrates: ATP; pyruvate; H2O
Specific reaction: ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate
General reaction: Phospho group transfer [C]
Inhibitor: 2-Oxoglutarate; 3-Phosphoglyceraldehyde; 5'-Adenylyl methylen ediphosphonate; ADP; ADP glucose; AMP; ATP; Ca2+; F-; Iodoacetate; Malate; Mg2+; Mn2+; Oxalacetate; PCMB; Phosphoenolpyruvate; Sulfhydryl reagents [C]
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9384377; 7704256 [H]